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The map label for this gene is mraW

Identifier: 28572698

GI number: 28572698

Start: 587070

End: 588023

Strand: Reverse

Name: mraW

Synonym: TW550

Alternate gene names: 28572698

Gene position: 588023-587070 (Counterclockwise)

Preceding gene: 28572699

Following gene: 28572697

Centisome position: 63.51

GC content: 45.6

Gene sequence:

>954_bases
ATGAATGGTCATTTGCCGGTTATGCTGGGCGAGGTCTGTCAGCTTATCGAGCCGGTTTTGGGAATTGGTGATGTGTTTAT
TGATGCAACGCTGGGAGCAGGAGGTCACAGTGAGGCAATTCTTATGTCCTCTCAAGGGGCGTTGCTCTATGGAATTGATC
GCGATGCTTACGCCCTGGCCCTAGCGCGCAAGCGCCTTTCCGGGTTTGCGGATCGCTGCAAATTTGTACATGACACCTTT
GATAAATTCGATAAATATTTATCTAATCTGCACCCAAAGGTGTTTTTGTTTGACCTTGGAATGTCATCTATGCAGATTGA
TAATCCAGACCGCGGTTTTTCTTATATGAAGTCTGGCCCCCTCGATATGCGTATGAACGAAAGTGACAAAATCACCGCAA
AGGAAATCCTTAATGGGTATTCGGAGACTGCTTTGATAAGAATTTTTAGGGATTACGGTCAGGAGCGCTACGCCAAGAGG
ATTGCTCGACAAATCTGTAAAGCAAGATCGGTAAGTGAGCTTGTTACAACATGTCAAGTGTCGCAGCTTATACGGGATGT
CTGCCCACCACATATCCGAAAGGGGCATCCAGCTAAGAGGGTATTTCAGGCGCTGCGCATTGAGGTGAATAGTGAGCTGT
TATTTCTGCGAACGGCACTTGAAAAAGCCTTGGATCTTTTGCAAGTTGGTGGGAGGATTGTTGTTTTGTCTTATCATTCG
CTTGAGGATCGCATTGTAAAACACTTGTTCAGAAGTGTGAGTGTTAGTCAATTGCCTAAGGGTTTTTATATTAACAAGGA
CCCAGAGTACAAATTAATTGGTAAGGATCTGAAAAATCCTAAAGAAACTGAGATTGCAAATAATCCGCGTGCAAGCAGCG
CTCATCTAAGGGCGGTGGAACGGGTTCATTCTCATTCACAAGCTGAAGCCCAGATAGTCGAGCCCCGCGCATGA

Upstream 100 bases:

>100_bases
GAAGCTGCTTTTTCTGAAATAGCCGAGGAGGTGATTCCGGGTCTTATTTGATGTCTGTGGCGATTCCCCGGCTACAGAAC
CTTATAAGGCCCGGTTTTAT

Downstream 100 bases:

>100_bases
TGTACAGGGTATCTGATATACGCTATAAGCGGCTGGTTATGTTTGCAGCAACTCTTGTTGTTGTCTTTGTTATTTCCCAG
TTGGCTTTGAGTTCTGTAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNGHLPVMLGEVCQLIEPVLGIGDVFIDATLGAGGHSEAILMSSQGALLYGIDRDAYALALARKRLSGFADRCKFVHDTF
DKFDKYLSNLHPKVFLFDLGMSSMQIDNPDRGFSYMKSGPLDMRMNESDKITAKEILNGYSETALIRIFRDYGQERYAKR
IARQICKARSVSELVTTCQVSQLIRDVCPPHIRKGHPAKRVFQALRIEVNSELLFLRTALEKALDLLQVGGRIVVLSYHS
LEDRIVKHLFRSVSVSQLPKGFYINKDPEYKLIGKDLKNPKETEIANNPRASSAHLRAVERVHSHSQAEAQIVEPRA

Sequences:

>Translated_317_residues
MNGHLPVMLGEVCQLIEPVLGIGDVFIDATLGAGGHSEAILMSSQGALLYGIDRDAYALALARKRLSGFADRCKFVHDTF
DKFDKYLSNLHPKVFLFDLGMSSMQIDNPDRGFSYMKSGPLDMRMNESDKITAKEILNGYSETALIRIFRDYGQERYAKR
IARQICKARSVSELVTTCQVSQLIRDVCPPHIRKGHPAKRVFQALRIEVNSELLFLRTALEKALDLLQVGGRIVVLSYHS
LEDRIVKHLFRSVSVSQLPKGFYINKDPEYKLIGKDLKNPKETEIANNPRASSAHLRAVERVHSHSQAEAQIVEPRA
>Mature_317_residues
MNGHLPVMLGEVCQLIEPVLGIGDVFIDATLGAGGHSEAILMSSQGALLYGIDRDAYALALARKRLSGFADRCKFVHDTF
DKFDKYLSNLHPKVFLFDLGMSSMQIDNPDRGFSYMKSGPLDMRMNESDKITAKEILNGYSETALIRIFRDYGQERYAKR
IARQICKARSVSELVTTCQVSQLIRDVCPPHIRKGHPAKRVFQALRIEVNSELLFLRTALEKALDLLQVGGRIVVLSYHS
LEDRIVKHLFRSVSVSQLPKGFYINKDPEYKLIGKDLKNPKETEIANNPRASSAHLRAVERVHSHSQAEAQIVEPRA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=37.6119402985075, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=39.1534391534392, Blast_Score=134, Evalue=9e-32,
Organism=Escherichia coli, GI1786270, Length=293, Percent_Identity=39.9317406143345, Blast_Score=198, Evalue=4e-52,
Organism=Drosophila melanogaster, GI62472493, Length=338, Percent_Identity=34.3195266272189, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI28571637, Length=338, Percent_Identity=34.3195266272189, Blast_Score=158, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_TROW8 (Q83HJ6)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789478.1
- ProteinModelPortal:   Q83HJ6
- SMR:   Q83HJ6
- STRING:   Q83HJ6
- GeneID:   1064753
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW550
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q83HJ6
- ProtClustDB:   PRK00050
- BioCyc:   TWHI218496:TW0523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35637; Mature: 35637

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: BINDING 53-53 BINDING 80-80 BINDING 98-98 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGHLPVMLGEVCQLIEPVLGIGDVFIDATLGAGGHSEAILMSSQGALLYGIDRDAYALA
CCCCCCHHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCCEEEEECCCCEEEECCCHHHHHH
LARKRLSGFADRCKFVHDTFDKFDKYLSNLHPKVFLFDLGMSSMQIDNPDRGFSYMKSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCHHHHCCC
LDMRMNESDKITAKEILNGYSETALIRIFRDYGQERYAKRIARQICKARSVSELVTTCQV
CCEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SQLIRDVCPPHIRKGHPAKRVFQALRIEVNSELLFLRTALEKALDLLQVGGRIVVLSYHS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
LEDRIVKHLFRSVSVSQLPKGFYINKDPEYKLIGKDLKNPKETEIANNPRASSAHLRAVE
HHHHHHHHHHHHCCHHHCCCCCEECCCCCCEECCCCCCCCCHHHCCCCCCCCHHHHHHHH
RVHSHSQAEAQIVEPRA
HHHCCCCCHHHCCCCCC
>Mature Secondary Structure
MNGHLPVMLGEVCQLIEPVLGIGDVFIDATLGAGGHSEAILMSSQGALLYGIDRDAYALA
CCCCCCHHHHHHHHHHHHHHHHHHHHEEHCCCCCCCCCEEEEECCCCEEEECCCHHHHHH
LARKRLSGFADRCKFVHDTFDKFDKYLSNLHPKVFLFDLGMSSMQIDNPDRGFSYMKSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCHHHHCCC
LDMRMNESDKITAKEILNGYSETALIRIFRDYGQERYAKRIARQICKARSVSELVTTCQV
CCEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SQLIRDVCPPHIRKGHPAKRVFQALRIEVNSELLFLRTALEKALDLLQVGGRIVVLSYHS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
LEDRIVKHLFRSVSVSQLPKGFYINKDPEYKLIGKDLKNPKETEIANNPRASSAHLRAVE
HHHHHHHHHHHHCCHHHCCCCCEECCCCCCEECCCCCCCCCHHHCCCCCCCCHHHHHHHH
RVHSHSQAEAQIVEPRA
HHHCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174