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The map label for this gene is def [H]

Identifier: 28572612

GI number: 28572612

Start: 499172

End: 499777

Strand: Direct

Name: def [H]

Synonym: TW462

Alternate gene names: 28572612

Gene position: 499172-499777 (Clockwise)

Preceding gene: 28572605

Following gene: 28572614

Centisome position: 53.91

GC content: 46.53

Gene sequence:

>606_bases
GTGCCTAAAATCAGCGGTGGCAAAATACTGCCAATATATATTACGGGTCACGCGGTTCTTCACGCCCCGGCAAAGCCTGT
AACCGACTTTTCTGGGATACAGGAAATTGTACGAGATATGTTTGCAACAATGTTTGCAGCGCCCGGAGTAGGGCTTGCTG
GGCCGCAGATCGGTCTTGGTTTAAGGATTTTTGTCTATTCCTACACCGAGGGTGATACCCTGCACCAGGGTGTTGCGATA
AATCCGGACTTGCTGATTCCAAAAGGTGTTCCTAAGCGACAGACACATAAGCAACAGGCAAATAACTCTACTTCTTGCGA
TGAGCCTGATAGAGAAGGTTGTCTTTCATTCCCGGGATATCAATTTCCCCTAGAAAGAGCTCCTCAAGTAACTCTCAGTG
CATTTGATGAAAATAAAAAGCCCTTTACTGTACATGCTACAGGGTGGCTTGCGCGCATTTTTCAACATGAATTCGATCAC
TTGCAAGGGACTTTATACGTGGATCGTCTTGCTCAAAAATATTCCGGAGAGGTTCGCCAAGCAGTTCTAAATAACAAGTG
GGGTATTCCGGGCAAATATTGGGTTCCGCAAGAGCCCAAGGAGTGA

Upstream 100 bases:

>100_bases
TTTTACTGTGAGTCAAGACGGCAAATACACCGGTTGCAATACACACAGCAATACACACAGCAATACACACAGCGCGCAAG
ATAGAGAAAAAGAAGGGGCC

Downstream 100 bases:

>100_bases
GAGCTTTGGACACTAATCCCCGGAGTTCCTGAGTTTTCCGCTCGCAATAGTGTGTCTGCAAGCAATCAAAACACCCACAA
TAATCATCGCAGCCGCACCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MPKISGGKILPIYITGHAVLHAPAKPVTDFSGIQEIVRDMFATMFAAPGVGLAGPQIGLGLRIFVYSYTEGDTLHQGVAI
NPDLLIPKGVPKRQTHKQQANNSTSCDEPDREGCLSFPGYQFPLERAPQVTLSAFDENKKPFTVHATGWLARIFQHEFDH
LQGTLYVDRLAQKYSGEVRQAVLNNKWGIPGKYWVPQEPKE

Sequences:

>Translated_201_residues
MPKISGGKILPIYITGHAVLHAPAKPVTDFSGIQEIVRDMFATMFAAPGVGLAGPQIGLGLRIFVYSYTEGDTLHQGVAI
NPDLLIPKGVPKRQTHKQQANNSTSCDEPDREGCLSFPGYQFPLERAPQVTLSAFDENKKPFTVHATGWLARIFQHEFDH
LQGTLYVDRLAQKYSGEVRQAVLNNKWGIPGKYWVPQEPKE
>Mature_200_residues
PKISGGKILPIYITGHAVLHAPAKPVTDFSGIQEIVRDMFATMFAAPGVGLAGPQIGLGLRIFVYSYTEGDTLHQGVAIN
PDLLIPKGVPKRQTHKQQANNSTSCDEPDREGCLSFPGYQFPLERAPQVTLSAFDENKKPFTVHATGWLARIFQHEFDHL
QGTLYVDRLAQKYSGEVRQAVLNNKWGIPGKYWVPQEPKE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=172, Percent_Identity=38.3720930232558, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645728, Length=174, Percent_Identity=32.183908045977, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24645726, Length=171, Percent_Identity=30.4093567251462, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22283; Mature: 22152

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKISGGKILPIYITGHAVLHAPAKPVTDFSGIQEIVRDMFATMFAAPGVGLAGPQIGLG
CCCCCCCEEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LRIFVYSYTEGDTLHQGVAINPDLLIPKGVPKRQTHKQQANNSTSCDEPDREGCLSFPGY
EEEEEEEECCCCCCCCCEECCCCEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QFPLERAPQVTLSAFDENKKPFTVHATGWLARIFQHEFDHLQGTLYVDRLAQKYSGEVRQ
CCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVLNNKWGIPGKYWVPQEPKE
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKISGGKILPIYITGHAVLHAPAKPVTDFSGIQEIVRDMFATMFAAPGVGLAGPQIGLG
CCCCCCEEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LRIFVYSYTEGDTLHQGVAINPDLLIPKGVPKRQTHKQQANNSTSCDEPDREGCLSFPGY
EEEEEEEECCCCCCCCCEECCCCEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QFPLERAPQVTLSAFDENKKPFTVHATGWLARIFQHEFDHLQGTLYVDRLAQKYSGEVRQ
CCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVLNNKWGIPGKYWVPQEPKE
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]