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The map label for this gene is cysS2

Identifier: 28572606

GI number: 28572606

Start: 493213

End: 494565

Strand: Reverse

Name: cysS2

Synonym: TW456

Alternate gene names: 28572606

Gene position: 494565-493213 (Counterclockwise)

Preceding gene: 28572607

Following gene: 28572604

Centisome position: 53.41

GC content: 44.35

Gene sequence:

>1353_bases
TTGTACACATTTGAGGTGTTTGATTCACGGACCAGGTCTATTGTAAAACTTGAAGATTGCTTACTGCGCTTATACGTCTG
TGGAATAACCCCGTACAAATCAACTCATCTCGGACACGCGTTTACGTATGTGGGCTTTGATACACTGTTTCGTTTGGCGT
TGGACTCTGGTAGAGATGTTCTGTACATTCAAAATATAAGTGATATCGATGAGCCTCTATTTCAATACGCAGAGAAAGTT
GGTATTCATTACAAAGACCTGGCCAGAACCCAGACCAAGCGTTTTTTTGAAGATATGCATACTCTTGAGTGCCTTCCGCC
AGGTTATGTTATTCCCGTTTCGAAGGTTCTAGACGGAATAAAAACAGGCATAGAGGGTCTTATCAGTCGTAATATGGCAT
ACAAACTGCCTAATGGAGATGTATATTTTGATTCAACCTTAACTGATCCGGGAAAAATGTTTTGTTTTGACAGAAAAACA
GCCATGAGTTTGATGCTTGAAACAGATACAGGTAAAAACCCATTTGACCCGCTTTTGTGGAGGGGGCGCGGCGCAGAGCC
ACAATGGGAGGCATCTTTTGGTGCGGGGAGACCCGCATGGCATATTTCATGCGCTGTCCTATCAAATTTACAGACACAAT
ACGAAAATGTGCTTCATATCTATGGTGGGGGCCGTGATCTTGCTTTCCCGCATCATGAATTTACAAATGTATTATCCAAA
TTGATTCGTGCGCCAAAGGATAATAAGCAACAGGATACTGTGCAAGATGTATTTATGCATACCGGACTTGTGTCGTATAT
GGGGGACAAGATGTCGAAGTCAAAGGGCAATCTTGTATTTATCTCACAATTACGCGAGCAATGCGAAAAAATTGGCCTGC
ATCACTCAGTTATCCGGTTAGCGCTGTTGCAAAGACACTACAGAGAGGATTGGGAGTGGCAGGATGAGTGTCTTGATCGG
GCGGCTTCCAGGTTCAGACTCTGGAAGAGTGCGTTACAAGAATATATTGGGGCGAAGGGTATTAGGTCTACCGCAGATCA
AAATAAAGGCACGCAAGGAGCCTGGGAGCGTATTCACGGTGGCGTATTTGACAGCAATTTTGACCACAGGCAGCCCATTC
ATCCTAAACATTCACCTCAAATGAGGGATTATTCTGAGCATGGTAGTGCGGGGCAGAACGGTACAGACTTAGATCTAAGT
CTGTATCAGGCAATACGATTTCATCTCTGCAATGATCTTGATACTCCAAAGGCCCTTGATGCTGTTGATAGCTACGCACG
AAAAGGTACAATAACTATTCCGGAAGCTCGCGCAGTCGAGAAATTGTTGGGAATTCCTCTGACTCGCGTCTGA

Upstream 100 bases:

>100_bases
ATAGGGTTTTGTTCGGGCTGTTTCTGGTAATTGTTTCCTTCTTTTAGTTGTTTTTCCGGCGCATTCCATGGAAGCCAATT
GGCCGTATACGGGGTAGCAT

Downstream 100 bases:

>100_bases
TACTTGATAGGTATCTTTCAAGACTCTTTACAAGATGGTCATTTGCAGTTGATGACAGGGCAACGCGGTCCTGCAGCTCT
TCTAGCTGTTTTATATATTC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase 2; CysRS 2

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MYTFEVFDSRTRSIVKLEDCLLRLYVCGITPYKSTHLGHAFTYVGFDTLFRLALDSGRDVLYIQNISDIDEPLFQYAEKV
GIHYKDLARTQTKRFFEDMHTLECLPPGYVIPVSKVLDGIKTGIEGLISRNMAYKLPNGDVYFDSTLTDPGKMFCFDRKT
AMSLMLETDTGKNPFDPLLWRGRGAEPQWEASFGAGRPAWHISCAVLSNLQTQYENVLHIYGGGRDLAFPHHEFTNVLSK
LIRAPKDNKQQDTVQDVFMHTGLVSYMGDKMSKSKGNLVFISQLREQCEKIGLHHSVIRLALLQRHYREDWEWQDECLDR
AASRFRLWKSALQEYIGAKGIRSTADQNKGTQGAWERIHGGVFDSNFDHRQPIHPKHSPQMRDYSEHGSAGQNGTDLDLS
LYQAIRFHLCNDLDTPKALDAVDSYARKGTITIPEARAVEKLLGIPLTRV

Sequences:

>Translated_450_residues
MYTFEVFDSRTRSIVKLEDCLLRLYVCGITPYKSTHLGHAFTYVGFDTLFRLALDSGRDVLYIQNISDIDEPLFQYAEKV
GIHYKDLARTQTKRFFEDMHTLECLPPGYVIPVSKVLDGIKTGIEGLISRNMAYKLPNGDVYFDSTLTDPGKMFCFDRKT
AMSLMLETDTGKNPFDPLLWRGRGAEPQWEASFGAGRPAWHISCAVLSNLQTQYENVLHIYGGGRDLAFPHHEFTNVLSK
LIRAPKDNKQQDTVQDVFMHTGLVSYMGDKMSKSKGNLVFISQLREQCEKIGLHHSVIRLALLQRHYREDWEWQDECLDR
AASRFRLWKSALQEYIGAKGIRSTADQNKGTQGAWERIHGGVFDSNFDHRQPIHPKHSPQMRDYSEHGSAGQNGTDLDLS
LYQAIRFHLCNDLDTPKALDAVDSYARKGTITIPEARAVEKLLGIPLTRV
>Mature_450_residues
MYTFEVFDSRTRSIVKLEDCLLRLYVCGITPYKSTHLGHAFTYVGFDTLFRLALDSGRDVLYIQNISDIDEPLFQYAEKV
GIHYKDLARTQTKRFFEDMHTLECLPPGYVIPVSKVLDGIKTGIEGLISRNMAYKLPNGDVYFDSTLTDPGKMFCFDRKT
AMSLMLETDTGKNPFDPLLWRGRGAEPQWEASFGAGRPAWHISCAVLSNLQTQYENVLHIYGGGRDLAFPHHEFTNVLSK
LIRAPKDNKQQDTVQDVFMHTGLVSYMGDKMSKSKGNLVFISQLREQCEKIGLHHSVIRLALLQRHYREDWEWQDECLDR
AASRFRLWKSALQEYIGAKGIRSTADQNKGTQGAWERIHGGVFDSNFDHRQPIHPKHSPQMRDYSEHGSAGQNGTDLDLS
LYQAIRFHLCNDLDTPKALDAVDSYARKGTITIPEARAVEKLLGIPLTRV

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=307, Percent_Identity=30.2931596091205, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI10835051, Length=300, Percent_Identity=26, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI62240992, Length=300, Percent_Identity=26, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI303304987, Length=300, Percent_Identity=26, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI21269877, Length=300, Percent_Identity=26, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786737, Length=315, Percent_Identity=31.4285714285714, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17509797, Length=260, Percent_Identity=28.4615384615385, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509795, Length=260, Percent_Identity=28.4615384615385, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324082, Length=215, Percent_Identity=32.093023255814, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922218, Length=329, Percent_Identity=32.8267477203647, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19922364, Length=256, Percent_Identity=28.125, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC2_TROW8 (Q83HQ8)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789386.1
- ProteinModelPortal:   Q83HQ8
- SMR:   Q83HQ8
- STRING:   Q83HQ8
- GeneID:   1064297
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW456
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   CLSK229740
- BioCyc:   TWHI218496:TW0431-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983

Pfam domain/function: PF01406 tRNA-synt_1e

EC number: =6.1.1.16

Molecular weight: Translated: 51275; Mature: 51275

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTFEVFDSRTRSIVKLEDCLLRLYVCGITPYKSTHLGHAFTYVGFDTLFRLALDSGRDV
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
LYIQNISDIDEPLFQYAEKVGIHYKDLARTQTKRFFEDMHTLECLPPGYVIPVSKVLDGI
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
KTGIEGLISRNMAYKLPNGDVYFDSTLTDPGKMFCFDRKTAMSLMLETDTGKNPFDPLLW
HHHHHHHHHCCCEEECCCCCEEECCCCCCCCCEEEECCHHHHEEEEECCCCCCCCCHHHC
RGRGAEPQWEASFGAGRPAWHISCAVLSNLQTQYENVLHIYGGGRDLAFPHHEFTNVLSK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHH
LIRAPKDNKQQDTVQDVFMHTGLVSYMGDKMSKSKGNLVFISQLREQCEKIGLHHSVIRL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCHHHHHHHH
ALLQRHYREDWEWQDECLDRAASRFRLWKSALQEYIGAKGIRSTADQNKGTQGAWERIHG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCC
GVFDSNFDHRQPIHPKHSPQMRDYSEHGSAGQNGTDLDLSLYQAIRFHLCNDLDTPKALD
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
AVDSYARKGTITIPEARAVEKLLGIPLTRV
HHHHHHHCCCEECCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYTFEVFDSRTRSIVKLEDCLLRLYVCGITPYKSTHLGHAFTYVGFDTLFRLALDSGRDV
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
LYIQNISDIDEPLFQYAEKVGIHYKDLARTQTKRFFEDMHTLECLPPGYVIPVSKVLDGI
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
KTGIEGLISRNMAYKLPNGDVYFDSTLTDPGKMFCFDRKTAMSLMLETDTGKNPFDPLLW
HHHHHHHHHCCCEEECCCCCEEECCCCCCCCCEEEECCHHHHEEEEECCCCCCCCCHHHC
RGRGAEPQWEASFGAGRPAWHISCAVLSNLQTQYENVLHIYGGGRDLAFPHHEFTNVLSK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHH
LIRAPKDNKQQDTVQDVFMHTGLVSYMGDKMSKSKGNLVFISQLREQCEKIGLHHSVIRL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCHHHHHHHH
ALLQRHYREDWEWQDECLDRAASRFRLWKSALQEYIGAKGIRSTADQNKGTQGAWERIHG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCC
GVFDSNFDHRQPIHPKHSPQMRDYSEHGSAGQNGTDLDLSLYQAIRFHLCNDLDTPKALD
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
AVDSYARKGTITIPEARAVEKLLGIPLTRV
HHHHHHHCCCEECCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174