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The map label for this gene is trmI [H]

Identifier: 28572604

GI number: 28572604

Start: 491355

End: 492323

Strand: Reverse

Name: trmI [H]

Synonym: TW454

Alternate gene names: 28572604

Gene position: 492323-491355 (Counterclockwise)

Preceding gene: 28572606

Following gene: 28572603

Centisome position: 53.17

GC content: 41.38

Gene sequence:

>969_bases
ATGTCCTTTAATAATTCGTATTTATTCGAAAATTTTGACTGGTCAGAGTTCGTATATCCGAAAAATAAAAAAAAGCTTGT
TCAATTAGTTCCGGGCAAAAGGATCGACTCATCATTTGGGGCAATAAATTGTGACCAGATACTTGGCCATCCGGAAGGTG
TTGTTGTAACAACCTGTAAAAACCATGAAGTTATTGCATTTCGTGCAACACTTGATAGTTACACAAGTCTTGCAAAGAGA
AAAACTGCCATTATTTTTCCTCAAGTTTGCGCCTTTATAATTGCCGTTGCGGACATAAGGCCAGGATTAAATGTCCTCGA
AGCTGGTGTTGGTTCGGGAGCTCTGACGATATGGCTTTTAAATGCTCTGGCAGGTTTTGGCACTCTTTATTCATTTGAGG
AGCGTAGAGAATTCTTAGCACTTGCTAAAAGAAATGTGAGTGACTTTCTTGGCTTTAAATCGCGAGGTAATCCGTTGAGT
AAAACATCTACACAGAGTGATCCGACCTGCGATCCGAGCTGTAACAATATCCCGGATAATTGGATTTGTGTTGAAGGTAA
TCTAAGGGAGAATATTTTTTTGAATCTTGAAAGTGGTAGCATCGACAGGGTTGTTCTGGATATGCTCAAGCCCTGGGAGT
GCGTTGAATCGGTGACAGTCGCACTGCGTCCGGGAGGTATTTTTGTCTGTTATGCAACTGGACTTATTCAAGTTTCTAGA
CTGGTTGAAACGCTGCGAAATACAGGATTTTATACCGAACCCATTGTTTATGAAACCCTCCAAAGAAAATGGCATATTGA
AGGCCTCTCGATACGGCCAAATCATTCTGGCATGAAGCCTGGCGGATTCTTGATAATGGTTAGGCGACTGTTCAAAAAAA
ATAGTTTACGGATAAGGCAGAAACCTATCCATAAACATAAGTTCGACAAAGATGACATTCCCGCCTGGCTTCCGGAGCAA
ATAGGCTAG

Upstream 100 bases:

>100_bases
CAAATGCGGCGATAAGAACCCGACAATCTAAAAAACTAGCCACACCACCTCTATTACAGACACCTATAGTTCCCAAATAC
CAATAATTTAAGATACTATT

Downstream 100 bases:

>100_bases
ACTGTTTATATTAGCTAATGGTTTCTTTGAAATTTGTGGTTCTGACCTTCGGGCTTGTTGCGCTCTTGTCGGGGTGCGCC
GATCAGGGTATCAAGGTTGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSFNNSYLFENFDWSEFVYPKNKKKLVQLVPGKRIDSSFGAINCDQILGHPEGVVVTTCKNHEVIAFRATLDSYTSLAKR
KTAIIFPQVCAFIIAVADIRPGLNVLEAGVGSGALTIWLLNALAGFGTLYSFEERREFLALAKRNVSDFLGFKSRGNPLS
KTSTQSDPTCDPSCNNIPDNWICVEGNLRENIFLNLESGSIDRVVLDMLKPWECVESVTVALRPGGIFVCYATGLIQVSR
LVETLRNTGFYTEPIVYETLQRKWHIEGLSIRPNHSGMKPGGFLIMVRRLFKKNSLRIRQKPIHKHKFDKDDIPAWLPEQ
IG

Sequences:

>Translated_322_residues
MSFNNSYLFENFDWSEFVYPKNKKKLVQLVPGKRIDSSFGAINCDQILGHPEGVVVTTCKNHEVIAFRATLDSYTSLAKR
KTAIIFPQVCAFIIAVADIRPGLNVLEAGVGSGALTIWLLNALAGFGTLYSFEERREFLALAKRNVSDFLGFKSRGNPLS
KTSTQSDPTCDPSCNNIPDNWICVEGNLRENIFLNLESGSIDRVVLDMLKPWECVESVTVALRPGGIFVCYATGLIQVSR
LVETLRNTGFYTEPIVYETLQRKWHIEGLSIRPNHSGMKPGGFLIMVRRLFKKNSLRIRQKPIHKHKFDKDDIPAWLPEQ
IG
>Mature_321_residues
SFNNSYLFENFDWSEFVYPKNKKKLVQLVPGKRIDSSFGAINCDQILGHPEGVVVTTCKNHEVIAFRATLDSYTSLAKRK
TAIIFPQVCAFIIAVADIRPGLNVLEAGVGSGALTIWLLNALAGFGTLYSFEERREFLALAKRNVSDFLGFKSRGNPLSK
TSTQSDPTCDPSCNNIPDNWICVEGNLRENIFLNLESGSIDRVVLDMLKPWECVESVTVALRPGGIFVCYATGLIQVSRL
VETLRNTGFYTEPIVYETLQRKWHIEGLSIRPNHSGMKPGGFLIMVRRLFKKNSLRIRQKPIHKHKFDKDDIPAWLPEQI
G

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI222831587, Length=242, Percent_Identity=28.099173553719, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 36228; Mature: 36097

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNNSYLFENFDWSEFVYPKNKKKLVQLVPGKRIDSSFGAINCDQILGHPEGVVVTTCK
CCCCCCEEEECCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEC
NHEVIAFRATLDSYTSLAKRKTAIIFPQVCAFIIAVADIRPGLNVLEAGVGSGALTIWLL
CCEEEEEEECHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHH
NALAGFGTLYSFEERREFLALAKRNVSDFLGFKSRGNPLSKTSTQSDPTCDPSCNNIPDN
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
WICVEGNLRENIFLNLESGSIDRVVLDMLKPWECVESVTVALRPGGIFVCYATGLIQVSR
EEEEECCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHEEEEECCCCEEEEEHHHHHHHHH
LVETLRNTGFYTEPIVYETLQRKWHIEGLSIRPNHSGMKPGGFLIMVRRLFKKNSLRIRQ
HHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEC
KPIHKHKFDKDDIPAWLPEQIG
CCCHHHCCCCCCCCCCCHHHCC
>Mature Secondary Structure 
SFNNSYLFENFDWSEFVYPKNKKKLVQLVPGKRIDSSFGAINCDQILGHPEGVVVTTCK
CCCCCEEEECCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEC
NHEVIAFRATLDSYTSLAKRKTAIIFPQVCAFIIAVADIRPGLNVLEAGVGSGALTIWLL
CCEEEEEEECHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHH
NALAGFGTLYSFEERREFLALAKRNVSDFLGFKSRGNPLSKTSTQSDPTCDPSCNNIPDN
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
WICVEGNLRENIFLNLESGSIDRVVLDMLKPWECVESVTVALRPGGIFVCYATGLIQVSR
EEEEECCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHEEEEECCCCEEEEEHHHHHHHHH
LVETLRNTGFYTEPIVYETLQRKWHIEGLSIRPNHSGMKPGGFLIMVRRLFKKNSLRIRQ
HHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEC
KPIHKHKFDKDDIPAWLPEQIG
CCCHHHCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA