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The map label for this gene is carA [H]

Identifier: 28572556

GI number: 28572556

Start: 438740

End: 439885

Strand: Direct

Name: carA [H]

Synonym: TW403

Alternate gene names: 28572556

Gene position: 438740-439885 (Clockwise)

Preceding gene: 28572555

Following gene: 28572557

Centisome position: 47.38

GC content: 46.25

Gene sequence:

>1146_bases
GTGCTTAGCTTTCGGTCTGCGGTATTCGTAATGGAAGATGGTTCTTGTTTTGAAGGTGTTCCATTTGGTGCTGTTGGTAC
TGCATTTGGCGAGGCCGTTTTTTCGACATGCATGACCGGGTATCAGGAAGTTTTTACAGACCCGAGTTACACAGCGCAAA
TTCTTGTTATGACAGCTGTGCATGTGGGTAACACAGGTATTAATGATTTTGATAACGAATCGGACAGAATGTGGCCAAGT
GGGGTTGTTATAAGGGATCCATCCCCAATCGCATCCAGTTTTCTTGCCAAAATGGATTTGGCAAAAGCCCTTGCTAGTGA
GGGAGTTATCGGAATTTCTGAGGTGGATACGCGCGCTATTGTTAGAAAATTACGCACATCAGGCGCCATGCGTGCCGGTA
TATTCTCGCAACCGGACCGACCTTTGCCCGATTTGGTGGATCAGATAAGGGGAACGCAGTCAATGCATATGCGTTCTCTA
ACATCCGAGGTGTCCACAACATCGCGCAGGTTTATCAGTTCTGAGTCCCATTTTGCAACTCTTCTTGTCTTAGATCTCGG
AGTGAAAAAAAGTACCATCAATTTGCTTCTAGAGCAGGGGTTCGATATTTGGCTTATGCCTCATAATATTTCCTTTGATG
AGGCTATGCAGATCAAACCTGATGCGATATTTTACTCAAATGGACCCGGTGATCCCGCGGCGAGTGGTACCCAGGTCGAT
CTCTTGCGCAGTCTGTTGAGAGCGGGTCTGCCCTACTTTGGTATTTGCTTTGGGAATCAATTGCTGGCCAGAGCCTTGGG
GTTTGAAACCAAGAAGCTTCTCTTTGGGCACCGGGGGATCAACCAACCCGTTTTCAACAGGGCAACAAAACAGGTTGAGA
TAACATCTCAAAATCATGGATTCTCGGTTGATATGCCAATCGATCGTATTTCTGTTTCACCTCTGAATTTTGGACGGGTC
GAAGTATCTCATTATTGTCTTAATGATTATGAAGTTGAAGGTATAAGATGCCTTGATATTCCCGCCATGTCAGTCCAGTA
TCATCCGGAATCTTGTGCCGGCCCGCATGACTCGCGCTATCTTTTTCGTGAATTTAGAAGAATGGTCGAAGGACACCTAG
AGCGAAGATACAATGCCGAACCCTAG

Upstream 100 bases:

>100_bases
ACTTACCGGGGAAGGTTATGTACACCTTTTATGAGGGTAGACTTGTTTTTGAAAACTAGGGCAAGATTGTCTTCTGCTTT
TGTGAGGTTTAAAAATAGGC

Downstream 100 bases:

>100_bases
AGATATAGGATCTGTTCTCGTAATCGGCTCTGGTCCTATAACTATAGGTCAAGCATGTGAGTTTGACTATTCAGGAACCC
AAGCTTGCAGGGTGTTGAAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLSFRSAVFVMEDGSCFEGVPFGAVGTAFGEAVFSTCMTGYQEVFTDPSYTAQILVMTAVHVGNTGINDFDNESDRMWPS
GVVIRDPSPIASSFLAKMDLAKALASEGVIGISEVDTRAIVRKLRTSGAMRAGIFSQPDRPLPDLVDQIRGTQSMHMRSL
TSEVSTTSRRFISSESHFATLLVLDLGVKKSTINLLLEQGFDIWLMPHNISFDEAMQIKPDAIFYSNGPGDPAASGTQVD
LLRSLLRAGLPYFGICFGNQLLARALGFETKKLLFGHRGINQPVFNRATKQVEITSQNHGFSVDMPIDRISVSPLNFGRV
EVSHYCLNDYEVEGIRCLDIPAMSVQYHPESCAGPHDSRYLFREFRRMVEGHLERRYNAEP

Sequences:

>Translated_381_residues
MLSFRSAVFVMEDGSCFEGVPFGAVGTAFGEAVFSTCMTGYQEVFTDPSYTAQILVMTAVHVGNTGINDFDNESDRMWPS
GVVIRDPSPIASSFLAKMDLAKALASEGVIGISEVDTRAIVRKLRTSGAMRAGIFSQPDRPLPDLVDQIRGTQSMHMRSL
TSEVSTTSRRFISSESHFATLLVLDLGVKKSTINLLLEQGFDIWLMPHNISFDEAMQIKPDAIFYSNGPGDPAASGTQVD
LLRSLLRAGLPYFGICFGNQLLARALGFETKKLLFGHRGINQPVFNRATKQVEITSQNHGFSVDMPIDRISVSPLNFGRV
EVSHYCLNDYEVEGIRCLDIPAMSVQYHPESCAGPHDSRYLFREFRRMVEGHLERRYNAEP
>Mature_381_residues
MLSFRSAVFVMEDGSCFEGVPFGAVGTAFGEAVFSTCMTGYQEVFTDPSYTAQILVMTAVHVGNTGINDFDNESDRMWPS
GVVIRDPSPIASSFLAKMDLAKALASEGVIGISEVDTRAIVRKLRTSGAMRAGIFSQPDRPLPDLVDQIRGTQSMHMRSL
TSEVSTTSRRFISSESHFATLLVLDLGVKKSTINLLLEQGFDIWLMPHNISFDEAMQIKPDAIFYSNGPGDPAASGTQVD
LLRSLLRAGLPYFGICFGNQLLARALGFETKKLLFGHRGINQPVFNRATKQVEITSQNHGFSVDMPIDRISVSPLNFGRV
EVSHYCLNDYEVEGIRCLDIPAMSVQYHPESCAGPHDSRYLFREFRRMVEGHLERRYNAEP

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=377, Percent_Identity=36.0742705570292, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI169790915, Length=378, Percent_Identity=33.0687830687831, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI21361331, Length=378, Percent_Identity=33.0687830687831, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=38.28125, Blast_Score=264, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI193204318, Length=376, Percent_Identity=35.9042553191489, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=34.1025641025641, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322331, Length=407, Percent_Identity=33.9066339066339, Blast_Score=196, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=33.9378238341969, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=33.9378238341969, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42183; Mature: 42183

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFRSAVFVMEDGSCFEGVPFGAVGTAFGEAVFSTCMTGYQEVFTDPSYTAQILVMTAV
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHEEEHH
HVGNTGINDFDNESDRMWPSGVVIRDPSPIASSFLAKMDLAKALASEGVIGISEVDTRAI
HHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
VRKLRTSGAMRAGIFSQPDRPLPDLVDQIRGTQSMHMRSLTSEVSTTSRRFISSESHFAT
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLVLDLGVKKSTINLLLEQGFDIWLMPHNISFDEAMQIKPDAIFYSNGPGDPAASGTQVD
EEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCEEEECCCCCCCCCCCCHHH
LLRSLLRAGLPYFGICFGNQLLARALGFETKKLLFGHRGINQPVFNRATKQVEITSQNHG
HHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHCCCEEEEEECCCC
FSVDMPIDRISVSPLNFGRVEVSHYCLNDYEVEGIRCLDIPAMSVQYHPESCAGPHDSRY
EEECCCHHHEEECCCCCCEEEEEEEECCCEEECCEEEECCCCCEEEECCHHCCCCCCHHH
LFREFRRMVEGHLERRYNAEP
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSFRSAVFVMEDGSCFEGVPFGAVGTAFGEAVFSTCMTGYQEVFTDPSYTAQILVMTAV
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHEEEHH
HVGNTGINDFDNESDRMWPSGVVIRDPSPIASSFLAKMDLAKALASEGVIGISEVDTRAI
HHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
VRKLRTSGAMRAGIFSQPDRPLPDLVDQIRGTQSMHMRSLTSEVSTTSRRFISSESHFAT
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLVLDLGVKKSTINLLLEQGFDIWLMPHNISFDEAMQIKPDAIFYSNGPGDPAASGTQVD
EEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCEEEECCCCCCCCCCCCHHH
LLRSLLRAGLPYFGICFGNQLLARALGFETKKLLFGHRGINQPVFNRATKQVEITSQNHG
HHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHCCCEEEEEECCCC
FSVDMPIDRISVSPLNFGRVEVSHYCLNDYEVEGIRCLDIPAMSVQYHPESCAGPHDSRY
EEECCCHHHEEECCCCCCEEEEEEEECCCEEECCEEEECCCCCEEEECCHHCCCCCCHHH
LFREFRRMVEGHLERRYNAEP
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]