Click here to switch to the map view.

The map label for this gene is sseA [H]

Identifier: 28572533

GI number: 28572533

Start: 410908

End: 411816

Strand: Reverse

Name: sseA [H]

Synonym: TW380

Alternate gene names: 28572533

Gene position: 411816-410908 (Counterclockwise)

Preceding gene: 28572534

Following gene: 28572532

Centisome position: 44.48

GC content: 48.18

Gene sequence:

>909_bases
TTGACGCGGTGTTTGTCGTGGACTGGGGGTTTTGTGAAAGAATTTCTTAGTGATGCAGAGAAGATATCTTCTTATGCAGA
TCCGAACCGATTGGTTACGACAGATTGGCTTGCCAAGAATCTTGATCGCGTGACTGTTATTGAGTCCGATGAGGATGTTC
TGCTGTACGAGAGCGGGCATATTCCTGGGTCTATAAAGGTTGACTGGCATACCGACTTGAACGACACGGTTCTGAGGGAT
TATCTGTCCCCGGAAAAGTTTCGCGATATGTGTGTATCAAAAGGTATTTCAGACAATGACATAATAGTTTTTTACGGAGA
CAAGTCAAACTGGTGGGCGGCGTACGCGGCGTGGGTATTTTGCTTGTACGGTAACGATAACTGGAAGCTACTTGATGGTG
GACGTGATAAATGGATATCGGAGGGTCGCCCGATGACAAAAGAAGTTGCCAATCGCCCGAGGGGTACGTATGCGCTGCCT
GTTCGCAACGATGATGAGATAAGGGCATACAGGGATGATGTCGCGAAACATATTGGCAAGCAAATGATTGATGTGCGTTC
AACAAGGGAATACACGGGCGAACTGACGCATATGCCAGAGTACCCGCAGGAAGGTGCTCTGCGAGGGGGACATGTCCCAA
GTGCTGTCTCAATCCCCTGGTCAAGCGCAGTTGGGAGCGATGGGACCTTTCTGCCTAGAGAAAAACTGGAAGAAATCTAT
TTCGGACAAACTGGTCACAGTGCTGATAAAGATACGATTGTCTATTGCCGGATCGGCGAGAGATCAAGTCACACCTGGTT
TGTTATGAAATACTTACTTGGTGTGAAGCATATAAGGAATTACGATGGCTCATGGACTGAGTGGGGTAATCTGGTGCGTG
CTCCTATCGTAACCGGCCCTAATCCCTAG

Upstream 100 bases:

>100_bases
ATATATTGACGGACTCTGGCCATGCTTGTAAAGGAGAATTGCCGGGAATATACTTGGAACGGTTGTGCTTGAATTGTTAC
CCTTGTGGTGACAAAGTGAT

Downstream 100 bases:

>100_bases
CTTTTGGTGACACGGCAGTTCCTTTGTTTCCATGGATCACGGGCGGTGTGCATTAGGGCGATTTTCTGTGTTTCGTCAGA
GAGTAAGGCTGATCTAAAAT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTRCLSWTGGFVKEFLSDAEKISSYADPNRLVTTDWLAKNLDRVTVIESDEDVLLYESGHIPGSIKVDWHTDLNDTVLRD
YLSPEKFRDMCVSKGISDNDIIVFYGDKSNWWAAYAAWVFCLYGNDNWKLLDGGRDKWISEGRPMTKEVANRPRGTYALP
VRNDDEIRAYRDDVAKHIGKQMIDVRSTREYTGELTHMPEYPQEGALRGGHVPSAVSIPWSSAVGSDGTFLPREKLEEIY
FGQTGHSADKDTIVYCRIGERSSHTWFVMKYLLGVKHIRNYDGSWTEWGNLVRAPIVTGPNP

Sequences:

>Translated_302_residues
MTRCLSWTGGFVKEFLSDAEKISSYADPNRLVTTDWLAKNLDRVTVIESDEDVLLYESGHIPGSIKVDWHTDLNDTVLRD
YLSPEKFRDMCVSKGISDNDIIVFYGDKSNWWAAYAAWVFCLYGNDNWKLLDGGRDKWISEGRPMTKEVANRPRGTYALP
VRNDDEIRAYRDDVAKHIGKQMIDVRSTREYTGELTHMPEYPQEGALRGGHVPSAVSIPWSSAVGSDGTFLPREKLEEIY
FGQTGHSADKDTIVYCRIGERSSHTWFVMKYLLGVKHIRNYDGSWTEWGNLVRAPIVTGPNP
>Mature_301_residues
TRCLSWTGGFVKEFLSDAEKISSYADPNRLVTTDWLAKNLDRVTVIESDEDVLLYESGHIPGSIKVDWHTDLNDTVLRDY
LSPEKFRDMCVSKGISDNDIIVFYGDKSNWWAAYAAWVFCLYGNDNWKLLDGGRDKWISEGRPMTKEVANRPRGTYALPV
RNDDEIRAYRDDVAKHIGKQMIDVRSTREYTGELTHMPEYPQEGALRGGHVPSAVSIPWSSAVGSDGTFLPREKLEEIYF
GQTGHSADKDTIVYCRIGERSSHTWFVMKYLLGVKHIRNYDGSWTEWGNLVRAPIVTGPNP

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=284, Percent_Identity=26.056338028169, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI61835204, Length=282, Percent_Identity=24.113475177305, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI194473681, Length=282, Percent_Identity=24.113475177305, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI194473668, Length=282, Percent_Identity=24.113475177305, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=26.0714285714286, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081967, Length=259, Percent_Identity=24.7104247104247, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17561888, Length=303, Percent_Identity=24.0924092409241, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71997283, Length=301, Percent_Identity=24.5847176079734, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 34396; Mature: 34265

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRCLSWTGGFVKEFLSDAEKISSYADPNRLVTTDWLAKNLDRVTVIESDEDVLLYESGH
CCCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCEEEEEECCCCEEEEECCC
IPGSIKVDWHTDLNDTVLRDYLSPEKFRDMCVSKGISDNDIIVFYGDKSNWWAAYAAWVF
CCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEEEEEE
CLYGNDNWKLLDGGRDKWISEGRPMTKEVANRPRGTYALPVRNDDEIRAYRDDVAKHIGK
EEEECCCEEEEECCCCHHHHCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
QMIDVRSTREYTGELTHMPEYPQEGALRGGHVPSAVSIPWSSAVGSDGTFLPREKLEEIY
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
FGQTGHSADKDTIVYCRIGERSSHTWFVMKYLLGVKHIRNYDGSWTEWGNLVRAPIVTGP
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCEEECCEECCC
NP
CC
>Mature Secondary Structure 
TRCLSWTGGFVKEFLSDAEKISSYADPNRLVTTDWLAKNLDRVTVIESDEDVLLYESGH
CCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCEEEEEECCCCEEEEECCC
IPGSIKVDWHTDLNDTVLRDYLSPEKFRDMCVSKGISDNDIIVFYGDKSNWWAAYAAWVF
CCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEEEEEE
CLYGNDNWKLLDGGRDKWISEGRPMTKEVANRPRGTYALPVRNDDEIRAYRDDVAKHIGK
EEEECCCEEEEECCCCHHHHCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
QMIDVRSTREYTGELTHMPEYPQEGALRGGHVPSAVSIPWSSAVGSDGTFLPREKLEEIY
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
FGQTGHSADKDTIVYCRIGERSSHTWFVMKYLLGVKHIRNYDGSWTEWGNLVRAPIVTGP
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCEEECCEECCC
NP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]