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The map label for this gene is gatB

Identifier: 28572511

GI number: 28572511

Start: 390777

End: 392291

Strand: Direct

Name: gatB

Synonym: TW357

Alternate gene names: 28572511

Gene position: 390777-392291 (Clockwise)

Preceding gene: 28572510

Following gene: 28572513

Centisome position: 42.2

GC content: 46.6

Gene sequence:

>1515_bases
GTGAGCGAGATTGTTACTTATGAGGATGCACTCAAGGATTTTGAGCCGATTATTGGCCTTGAGGTGCATGTTGAACTTTC
GACGCAAACCAAATTGTTTTCTTCAGCCCCCAATATTGCCGGACCCCTTGCCTCTTGCGCATCGCAAGGGCCCAACACTC
TTGTAACTCCTGTTTGCTTGGGGTTGCCTGGCTCCTTGCCGACCGTAAACGAACGAGCTGTCGATTATGGCATAGCCCTG
GGTCTCGCTCTTGGCTGTTCTATTGCAGATGAATTAATATTCGCGAGAAAGAATTATTTTTATCCCGATCTGCCAAAAAA
TTATCAGATATCACAATTTGATTCTCCACTGGCATATGACGGAAAACTTGAGGTTGAAACGGAAAGCGGTGATGTGTTTT
TTGTTGAAATCGAGAGGGCACATCTTGAGGAAGATGCCGGGAAACTTGCCCATAAGAGTTCAACCGGCAGAATTCAGGGT
GCACAGTATTCTCTAATTGATTACAACCGTTCCGGTGTGCCTCTAGTTGAAATTGTTTCTAGACCTGTTTTTGCCGATAG
GTGCGCCCCCCAGGTGGCGAGGGCGTTTGTTGAACTGATTAGGGAAATTGTCCTTTCCCTTGGCGTGTCTAATGCTCGGT
TAGAGCGGGGAAATATTCGGTGCGATGCAAATGTTTCTCTTCGCCCACGTGGGCTGGGTGCAGGCATTCTACCTAATAGA
ACCGAAACTAAGAACCTAAATTCCCTGCGTTCTATTGAGCGGGCTATTAGATACGAAATTCAGCGACAAGCAACACTTAT
TTCTTCTGGCGAGCTTGTACGGCAAGAAACACGGCATTGGCGCGAGGATCGGGGCATAACCCTATCAGGTCGTGTAAAAG
CTGATTCTCATGAGTATCGCTATTTTCCTGAACCTGATCTTTTGCCCATTCCTATTCCTTCGGACAAAGTAGAGGCAATC
AGGTTATCGATGCCAGAGCACCCCTTGGCACTTCGTCGTCGCCTTAAGGCTGAATGGAAATTTAGTGATCTTCAGTTTCG
TGATGTACTAAATTCCTCGGTTCTAAAACAAGTTGATGAAACTGTCAGAGCAGGCGCAACGCCAGATGGCGCAAAAAAAT
GGTGGACAGGTGAGATTACGAGAATTGCTTCCCAACGACGCTGCAACGCTTCAGATTTGATCTCCCCGGAGGCAGTTGCA
GAGATTGAGTTACTAATAAGTAAGGGAATCCTGAATGACAGCCTTGCAAGAAAGGTACTTGCGGCTGTTATTGATGAGTC
ATTGAGCGTACAGCAAGCGATCGAAAAATACGATTTATCTCTCACAGAGAGCGCTTCTGTAGAGAGAGTTCTTGAGAAAG
TTCTTAGTGAAAATCAGGAAGTTGTACAAAAGGTAATTTCTGGCAAAACCCAAGCTGTCGGCGTATTGGTCGGCAGCTGT
ATGAAGACCCTGGGTGGTAAGGCAGATGCCTCAAAGCTTCGACAGACTATCCTGAATAGACTCCTTCCACGGTAA

Upstream 100 bases:

>100_bases
AATTTGACAGTAGGGTATATTCTACGGGCGCTGCAATCGAACAGATAATCGGGGACATACATGCAATGAAAAACACAAAG
CATAATACGGGGCAAACAGC

Downstream 100 bases:

>100_bases
GCTCTTGCGGTAAAGGCTTGTATCACTTTGTGCTGTTTTCGACAGTGATCAGGTGTGCTTGAGTCCCATGAGAATCCCCT
TGTGATCACTGCCCTCTAGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSEIVTYEDALKDFEPIIGLEVHVELSTQTKLFSSAPNIAGPLASCASQGPNTLVTPVCLGLPGSLPTVNERAVDYGIAL
GLALGCSIADELIFARKNYFYPDLPKNYQISQFDSPLAYDGKLEVETESGDVFFVEIERAHLEEDAGKLAHKSSTGRIQG
AQYSLIDYNRSGVPLVEIVSRPVFADRCAPQVARAFVELIREIVLSLGVSNARLERGNIRCDANVSLRPRGLGAGILPNR
TETKNLNSLRSIERAIRYEIQRQATLISSGELVRQETRHWREDRGITLSGRVKADSHEYRYFPEPDLLPIPIPSDKVEAI
RLSMPEHPLALRRRLKAEWKFSDLQFRDVLNSSVLKQVDETVRAGATPDGAKKWWTGEITRIASQRRCNASDLISPEAVA
EIELLISKGILNDSLARKVLAAVIDESLSVQQAIEKYDLSLTESASVERVLEKVLSENQEVVQKVISGKTQAVGVLVGSC
MKTLGGKADASKLRQTILNRLLPR

Sequences:

>Translated_504_residues
MSEIVTYEDALKDFEPIIGLEVHVELSTQTKLFSSAPNIAGPLASCASQGPNTLVTPVCLGLPGSLPTVNERAVDYGIAL
GLALGCSIADELIFARKNYFYPDLPKNYQISQFDSPLAYDGKLEVETESGDVFFVEIERAHLEEDAGKLAHKSSTGRIQG
AQYSLIDYNRSGVPLVEIVSRPVFADRCAPQVARAFVELIREIVLSLGVSNARLERGNIRCDANVSLRPRGLGAGILPNR
TETKNLNSLRSIERAIRYEIQRQATLISSGELVRQETRHWREDRGITLSGRVKADSHEYRYFPEPDLLPIPIPSDKVEAI
RLSMPEHPLALRRRLKAEWKFSDLQFRDVLNSSVLKQVDETVRAGATPDGAKKWWTGEITRIASQRRCNASDLISPEAVA
EIELLISKGILNDSLARKVLAAVIDESLSVQQAIEKYDLSLTESASVERVLEKVLSENQEVVQKVISGKTQAVGVLVGSC
MKTLGGKADASKLRQTILNRLLPR
>Mature_503_residues
SEIVTYEDALKDFEPIIGLEVHVELSTQTKLFSSAPNIAGPLASCASQGPNTLVTPVCLGLPGSLPTVNERAVDYGIALG
LALGCSIADELIFARKNYFYPDLPKNYQISQFDSPLAYDGKLEVETESGDVFFVEIERAHLEEDAGKLAHKSSTGRIQGA
QYSLIDYNRSGVPLVEIVSRPVFADRCAPQVARAFVELIREIVLSLGVSNARLERGNIRCDANVSLRPRGLGAGILPNRT
ETKNLNSLRSIERAIRYEIQRQATLISSGELVRQETRHWREDRGITLSGRVKADSHEYRYFPEPDLLPIPIPSDKVEAIR
LSMPEHPLALRRRLKAEWKFSDLQFRDVLNSSVLKQVDETVRAGATPDGAKKWWTGEITRIASQRRCNASDLISPEAVAE
IELLISKGILNDSLARKVLAAVIDESLSVQQAIEKYDLSLTESASVERVLEKVLSENQEVVQKVISGKTQAVGVLVGSCM
KTLGGKADASKLRQTILNRLLPR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=510, Percent_Identity=31.1764705882353, Blast_Score=208, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115534094, Length=305, Percent_Identity=39.0163934426229, Blast_Score=188, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6319391, Length=522, Percent_Identity=28.3524904214559, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI45550808, Length=342, Percent_Identity=34.7953216374269, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_TROW8 (Q83HX1)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789291.1
- ProteinModelPortal:   Q83HX1
- SMR:   Q83HX1
- STRING:   Q83HX1
- GeneID:   1064644
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW357
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   RIHGATH
- ProtClustDB:   PRK05477
- BioCyc:   TWHI218496:TW0336-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55623; Mature: 55492

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIVTYEDALKDFEPIIGLEVHVELSTQTKLFSSAPNIAGPLASCASQGPNTLVTPVCL
CCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCCHHHHHHHHCCCCCCEECHHHH
GLPGSLPTVNERAVDYGIALGLALGCSIADELIFARKNYFYPDLPKNYQISQFDSPLAYD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEC
GKLEVETESGDVFFVEIERAHLEEDAGKLAHKSSTGRIQGAQYSLIDYNRSGVPLVEIVS
CEEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHC
RPVFADRCAPQVARAFVELIREIVLSLGVSNARLERGNIRCDANVSLRPRGLGAGILPNR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCEECCCCCCCCCCCCC
TETKNLNSLRSIERAIRYEIQRQATLISSGELVRQETRHWREDRGITLSGRVKADSHEYR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECEEECCCCCCC
YFPEPDLLPIPIPSDKVEAIRLSMPEHPLALRRRLKAEWKFSDLQFRDVLNSSVLKQVDE
CCCCCCCEECCCCCCCHHEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TVRAGATPDGAKKWWTGEITRIASQRRCNASDLISPEAVAEIELLISKGILNDSLARKVL
HHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
AAVIDESLSVQQAIEKYDLSLTESASVERVLEKVLSENQEVVQKVISGKTQAVGVLVGSC
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
MKTLGGKADASKLRQTILNRLLPR
HHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIVTYEDALKDFEPIIGLEVHVELSTQTKLFSSAPNIAGPLASCASQGPNTLVTPVCL
CCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCCHHHHHHHHCCCCCCEECHHHH
GLPGSLPTVNERAVDYGIALGLALGCSIADELIFARKNYFYPDLPKNYQISQFDSPLAYD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEC
GKLEVETESGDVFFVEIERAHLEEDAGKLAHKSSTGRIQGAQYSLIDYNRSGVPLVEIVS
CEEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHC
RPVFADRCAPQVARAFVELIREIVLSLGVSNARLERGNIRCDANVSLRPRGLGAGILPNR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCEECCCCCCCCCCCCC
TETKNLNSLRSIERAIRYEIQRQATLISSGELVRQETRHWREDRGITLSGRVKADSHEYR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECEEECCCCCCC
YFPEPDLLPIPIPSDKVEAIRLSMPEHPLALRRRLKAEWKFSDLQFRDVLNSSVLKQVDE
CCCCCCCEECCCCCCCHHEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TVRAGATPDGAKKWWTGEITRIASQRRCNASDLISPEAVAEIELLISKGILNDSLARKVL
HHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
AAVIDESLSVQQAIEKYDLSLTESASVERVLEKVLSENQEVVQKVISGKTQAVGVLVGSC
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
MKTLGGKADASKLRQTILNRLLPR
HHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174