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The map label for this gene is gatA [H]

Identifier: 28572510

GI number: 28572510

Start: 389299

End: 390780

Strand: Direct

Name: gatA [H]

Synonym: TW356

Alternate gene names: 28572510

Gene position: 389299-390780 (Clockwise)

Preceding gene: 28572509

Following gene: 28572511

Centisome position: 42.04

GC content: 46.83

Gene sequence:

>1482_bases
GTGCTTTCTGAAATGCCGGCTCACAGGCTGGCCGCCCTGATTCGCTCGAAAGAAGTAAGTGCACTTGAGGTCGCTGAGAG
TTTTATAGACAATATTGAAGCCTCGGACTCGCGCATATGCGCATTTCTATATACAGATTTTTCATATACCCGTGACGTTG
CAAGAAGGGTAGATGAAGAGCTAAAGTCTGCAACAAAACTTTCGCCATTAGCCGGCGTGCCAATTGCCGTGAAAGACATG
ATTCTAACAAGGGATATGCCCACAACCGCCGGATCAAAGATTCTGGAAGGGTGGATTCCACCGTACAACGCTACTGTAAT
AGAGCGTATTTCTCGTGCCCGTATGCCAATTCTAGGAAAAACGAATCAGGATGAATTTGGGATGGGCTCATCTACCGAAT
ATTCTGCCTACAAAACGACAAGAAATCCGTGGGATTTGAGCAGGACTGCGGGCGGCTCAGGGGGTGGTTCCTCTGCGGCT
GTGTCAGCTTCCCAGGCACCTTTGGCTCTAGGTACTGATACCGGTGGATCTATTAGATTACCCGCTCACTGTACCGGAAC
TGTCGGGATAAGGCCAACGTATGGGAGTGTCAGTCGCTATGGAGTAATTGCCCTTGCAAGTTCCTTTGATCAGGTTGGTC
CTTGCTCTAGCAATATTCTTGATGCCGCCCTCTTGCACGAGGTTATTGCTGGGTATGATCCTGCGGATGCTGTCTCTATA
AAAGATCAGGATCTAAACTTTTCGCAAGCTGCCTATGAAGGTGCTAACAGGGGTATTTCTGGGGTTAGGCTAGGTTTCGT
AAACCCAAGCAATTGGTGTAATTCAAAAATCACAGACCTTTTTGGAAGAACTTTGAAGAGCTTGGAGTCTGAGGGTGCAG
TTCTGCATGAGGTACAGTTTCCGAATTTTGACCACGCGGTTCAAGCTTATTATTTGATAATGCAGGCCGAAGCATCCTCG
AACCTGTCTCGTTATGATTCTATTCGGTTTGGTCCACAGGAGATGGCAGCTTCTGCAAGTGGGACTGTATCAAAAACTCG
GTCTATTCGGTTTGGTCCCGAGGTCAAGAGGAGAATTCTCCTTGGCACTCATATTCTTTCTGCCGGTTATTATGATGACT
TTTATATGTCAGCTCAAAAAATTCGTTCATTGGTAAAGCGTGACTTTGCCAAGATATTTTCCTTAGTTGATGTTCTTCTT
TTGCCAACGGCACCCACGCCCGCCTTTAAACTCGGCGAAAAGATTGACCATCATACTTCCATGTATAAGTCCGACACAGC
GACCACTCCGGCCAGTTTAGCTGGTTTACCAGCCGGAAGTATTCCTATGGGAGTGATTGATGGTTTACCTGTTGGGTTAC
AGATAATTGCACCCGGTCAATTTGACAGTAGGGTATATTCTACGGGCGCTGCAATCGAACAGATAATCGGGGACATACAT
GCAATGAAAAACACAAAGCATAATACGGGGCAAACAGCGTGA

Upstream 100 bases:

>100_bases
TTTCGCCATGATACGCCGGGCCCCGTTCTTGATATTGCTCCGGTTCTGTCGAATGGGGAAGATGGCATGTTCCGGATATC
CGCCATTTCAGGGGAGGATA

Downstream 100 bases:

>100_bases
GCGAGATTGTTACTTATGAGGATGCACTCAAGGATTTTGAGCCGATTATTGGCCTTGAGGTGCATGTTGAACTTTCGACG
CAAACCAAATTGTTTTCTTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MLSEMPAHRLAALIRSKEVSALEVAESFIDNIEASDSRICAFLYTDFSYTRDVARRVDEELKSATKLSPLAGVPIAVKDM
ILTRDMPTTAGSKILEGWIPPYNATVIERISRARMPILGKTNQDEFGMGSSTEYSAYKTTRNPWDLSRTAGGSGGGSSAA
VSASQAPLALGTDTGGSIRLPAHCTGTVGIRPTYGSVSRYGVIALASSFDQVGPCSSNILDAALLHEVIAGYDPADAVSI
KDQDLNFSQAAYEGANRGISGVRLGFVNPSNWCNSKITDLFGRTLKSLESEGAVLHEVQFPNFDHAVQAYYLIMQAEASS
NLSRYDSIRFGPQEMAASASGTVSKTRSIRFGPEVKRRILLGTHILSAGYYDDFYMSAQKIRSLVKRDFAKIFSLVDVLL
LPTAPTPAFKLGEKIDHHTSMYKSDTATTPASLAGLPAGSIPMGVIDGLPVGLQIIAPGQFDSRVYSTGAAIEQIIGDIH
AMKNTKHNTGQTA

Sequences:

>Translated_493_residues
MLSEMPAHRLAALIRSKEVSALEVAESFIDNIEASDSRICAFLYTDFSYTRDVARRVDEELKSATKLSPLAGVPIAVKDM
ILTRDMPTTAGSKILEGWIPPYNATVIERISRARMPILGKTNQDEFGMGSSTEYSAYKTTRNPWDLSRTAGGSGGGSSAA
VSASQAPLALGTDTGGSIRLPAHCTGTVGIRPTYGSVSRYGVIALASSFDQVGPCSSNILDAALLHEVIAGYDPADAVSI
KDQDLNFSQAAYEGANRGISGVRLGFVNPSNWCNSKITDLFGRTLKSLESEGAVLHEVQFPNFDHAVQAYYLIMQAEASS
NLSRYDSIRFGPQEMAASASGTVSKTRSIRFGPEVKRRILLGTHILSAGYYDDFYMSAQKIRSLVKRDFAKIFSLVDVLL
LPTAPTPAFKLGEKIDHHTSMYKSDTATTPASLAGLPAGSIPMGVIDGLPVGLQIIAPGQFDSRVYSTGAAIEQIIGDIH
AMKNTKHNTGQTA
>Mature_493_residues
MLSEMPAHRLAALIRSKEVSALEVAESFIDNIEASDSRICAFLYTDFSYTRDVARRVDEELKSATKLSPLAGVPIAVKDM
ILTRDMPTTAGSKILEGWIPPYNATVIERISRARMPILGKTNQDEFGMGSSTEYSAYKTTRNPWDLSRTAGGSGGGSSAA
VSASQAPLALGTDTGGSIRLPAHCTGTVGIRPTYGSVSRYGVIALASSFDQVGPCSSNILDAALLHEVIAGYDPADAVSI
KDQDLNFSQAAYEGANRGISGVRLGFVNPSNWCNSKITDLFGRTLKSLESEGAVLHEVQFPNFDHAVQAYYLIMQAEASS
NLSRYDSIRFGPQEMAASASGTVSKTRSIRFGPEVKRRILLGTHILSAGYYDDFYMSAQKIRSLVKRDFAKIFSLVDVLL
LPTAPTPAFKLGEKIDHHTSMYKSDTATTPASLAGLPAGSIPMGVIDGLPVGLQIIAPGQFDSRVYSTGAAIEQIIGDIH
AMKNTKHNTGQTA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=31, Blast_Score=230, Evalue=3e-60,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=24.1869918699187, Blast_Score=120, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=445, Percent_Identity=33.2584269662921, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17537465, Length=506, Percent_Identity=23.3201581027668, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=146, Percent_Identity=33.5616438356164, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=140, Percent_Identity=32.1428571428571, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=193, Percent_Identity=26.9430051813472, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=425, Percent_Identity=35.0588235294118, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319685, Length=393, Percent_Identity=30.2798982188295, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648113, Length=441, Percent_Identity=35.1473922902494, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI45550774, Length=207, Percent_Identity=34.7826086956522, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=207, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648437, Length=207, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=25.4582484725051, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=25.4582484725051, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=25.4582484725051, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=25.4582484725051, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=23.4939759036145, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=24.3137254901961, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356731, Length=493, Percent_Identity=21.501014198783, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=141, Percent_Identity=37.5886524822695, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=141, Percent_Identity=37.5886524822695, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52849; Mature: 52849

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEMPAHRLAALIRSKEVSALEVAESFIDNIEASDSRICAFLYTDFSYTRDVARRVDEE
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
LKSATKLSPLAGVPIAVKDMILTRDMPTTAGSKILEGWIPPYNATVIERISRARMPILGK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
TNQDEFGMGSSTEYSAYKTTRNPWDLSRTAGGSGGGSSAAVSASQAPLALGTDTGGSIRL
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEEEE
PAHCTGTVGIRPTYGSVSRYGVIALASSFDQVGPCSSNILDAALLHEVIAGYDPADAVSI
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
KDQDLNFSQAAYEGANRGISGVRLGFVNPSNWCNSKITDLFGRTLKSLESEGAVLHEVQF
ECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PNFDHAVQAYYLIMQAEASSNLSRYDSIRFGPQEMAASASGTVSKTRSIRFGPEVKRRIL
CCHHHHHHHHHHEEECCCCCCHHHHHCCCCCHHHHHHCCCCCHHHCCCEECCHHHHHHHH
LGTHILSAGYYDDFYMSAQKIRSLVKRDFAKIFSLVDVLLLPTAPTPAFKLGEKIDHHTS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
MYKSDTATTPASLAGLPAGSIPMGVIDGLPVGLQIIAPGQFDSRVYSTGAAIEQIIGDIH
HHCCCCCCCCHHHCCCCCCCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AMKNTKHNTGQTA
HHHCCCCCCCCCC
>Mature Secondary Structure
MLSEMPAHRLAALIRSKEVSALEVAESFIDNIEASDSRICAFLYTDFSYTRDVARRVDEE
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
LKSATKLSPLAGVPIAVKDMILTRDMPTTAGSKILEGWIPPYNATVIERISRARMPILGK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
TNQDEFGMGSSTEYSAYKTTRNPWDLSRTAGGSGGGSSAAVSASQAPLALGTDTGGSIRL
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEEEE
PAHCTGTVGIRPTYGSVSRYGVIALASSFDQVGPCSSNILDAALLHEVIAGYDPADAVSI
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
KDQDLNFSQAAYEGANRGISGVRLGFVNPSNWCNSKITDLFGRTLKSLESEGAVLHEVQF
ECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PNFDHAVQAYYLIMQAEASSNLSRYDSIRFGPQEMAASASGTVSKTRSIRFGPEVKRRIL
CCHHHHHHHHHHEEECCCCCCHHHHHCCCCCHHHHHHCCCCCHHHCCCEECCHHHHHHHH
LGTHILSAGYYDDFYMSAQKIRSLVKRDFAKIFSLVDVLLLPTAPTPAFKLGEKIDHHTS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
MYKSDTATTPASLAGLPAGSIPMGVIDGLPVGLQIIAPGQFDSRVYSTGAAIEQIIGDIH
HHCCCCCCCCHHHCCCCCCCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AMKNTKHNTGQTA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174 [H]