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The map label for this gene is pnp [H]

Identifier: 28572501

GI number: 28572501

Start: 377107

End: 379314

Strand: Direct

Name: pnp [H]

Synonym: TW347

Alternate gene names: 28572501

Gene position: 377107-379314 (Clockwise)

Preceding gene: 28572500

Following gene: 28572502

Centisome position: 40.73

GC content: 49.55

Gene sequence:

>2208_bases
GTGCAGTTTTCTGAGGGAGTGATAGATAACGGACGGTTTGGCACTCGCACGATTAGGTTCGAGACTGGCCGTCTCGCGAG
GCAGGCTCAGGGCTCCGTCGTTGCTTATCTGGATGGCGAGACGATGCTTTTGTCTTCTACCAGCGTCGGCAAGCAGCCGA
AGGAAGATTGTGATTTTTTCCCGCTCACGGTTGATGTCGAGGAGCGTTCCTATGCTGCAGGAAAAATACCGGGTTCATAC
TTTCGTCGTGAGGGAAGGCCCTCCACAGAGGCTATTCTTGCATGCAGGCTGATTGACAGGCCCCTGCGGCCCAGTTTTAA
TGCCGGGCTGCGGAATGAGGTTCAGGTAATAGTTACTGTTCTTAGCATTGCTCCAGGCGAATTCTATGAAGCTTTGGCAA
TAAATGCGGCGAGTGCATCAACCCTTGTATCTGGCCTGCCTTTCTCTGGCCCAATTGGCGGTGTGCGTCTCGCTCTTATA
GATGGTCAGTGGGTTGCCTTTCCGAGATATGAAGACCTGTCCGGGGCCGTGTTTGACCTAACTGTTGCAGGACGGGTGTT
TGTCAATGAGTCTGGCAAGGAAGATATTGCAATCATGATGGTTGAGGCAGAGGCCACAGAACCTGCCTGGGATCTTATTC
ATTCTCATGGCGCTAAAAAGCCCAATGAGGAGGTTATTGAGGAAGGAATCGAGTCAGCAAAGGTCTTTATACGTACCCTG
TGTGACATACAGCGTGATTTGGCAAGCAAGTTGTCATTGAGCCATACGGAGCCGGATTTGTATCCGGATTATTCCGATAG
TGTACATTCCTTTGTGGAGGGTCTGGTTAAATCTGACCTCGAGAAAGTCTATCGGGGCAATCATGATTCGGGATACGCTC
CGAATGCTTTATCTGCGTCCGATGAGATAAAGCAAAAAGCGTATGATGCTTTTCATGACGCAGTGCTCTCGGGTCGATTC
GATCAAGATAGCCTTTCTCAGTTTCCGCATGCGTACAAGGCGGTCCTAAAAGATGTTGTTAGAACTTGCGTTCTTGAGGG
TTTTGCCCGAATGGATGGTCGTGGGCTTTCCGATATACGCCCCTTGGATGCGGAGGTCCAAGTTGTCCCTCGCGTTCATG
GATCGGCTGTATTTCAGAGGGGCGAGACACAGGTCTTGGGCGTTACAACCCTTAATATGCTCAAAATGGAGCAGCAGATA
GATTCTTTGGCGCCGATTGTATCAAAGCGGTATATTCATCACTACAATTTCCCCCCGTATTCAACGGGCGAAGTCGGTCG
TGTTGGTAGTCCGAAGCGCAGGGAGATAGGTCACGGCTTCCTTGCCGAGCGCGCACTTGTTCCGGTTCTGCCGAGCCGCG
AAGATTTTCCGTATGCAATCCGACAGGTTTCAGAGGCATTGGGCTCCAACGGCTCCACATCCATGGGTTCTGTTTGTGCC
TCGACATTATCGCTTCTGAATGCCGGTGTTCCACTTCGGGCGCCAGTTGCTGGAATAGCTATGGGTCTTATCAGCGGTCG
TGTTGATGGCGAAATGCGATACGTTACGCTAACGGATATTTCCGGCTCGGAAGATGCTTTGGGGGATATGGATTTTAAAG
TTGCTGGGACATCTGATTTTATAACTGCGCTTCAGCTTGATACGAAGCTCGATGGGATTCCAGCACATGTTTTGAGTGAG
GCTCTGGCACATGCCCGGTCTGCGCGTTTGGCAATTCTAGATGTTTTAACTCGCGTAATAGATTCTCCCGATCAGATGTC
GGAATATGCCCCCCGTGTTGTAAGAGTAAAGATTCCTGTTCAAAAGATTGGCGAGCTGATTGGTCCAAAGGGTAAGGTGA
TAAATTCCATTCAGGATGAGACAGGTGCGGAAATTTCTATCGAGGATGATGGAACGGTGTATATAGGATCATCTCAGGCC
GATTCTTCAGAGAAAGCCGTCGCAATGGTGAATTCCATTGTAAACCCAGTAGAGCCTTGCGTTGGTAGCCAGTTTCTCGG
GACTGTTGTAAAAAATATGCCCTTCGGTTCTTTTATAAGTCTTGTTCCTGGTAAGGATGGCTTGCTTCATATATCTGAGA
TACGTAAGATGGTAGACGGGAGACATCTTGAAAGTGTCGATGAGGTCCTGAGCGTTGGTCAGAAGGTGCTTGTTGAGGTA
TCAAAGATCGACGATAGGGGTAAATTGTGCCTTGTCGCTGTCAAGTAG

Upstream 100 bases:

>100_bases
GGGTTGATGTTAGCCGGTATCGCGCGATTCTCAGCAACCTCGGTTTGCGCCGCTAGGTTTGGCTTTGTACTTCGTGGATT
GGATTTTCTAGTTGGATGAC

Downstream 100 bases:

>100_bases
CCGCGCTCCTGCTTTGGCGCTAGTTTCTTGTTTGGGGTCCTTTTGCATGGTCATACGCATTCTTCTTTTGATGTACATAT
AAACGTATCTATTTATAAAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MQFSEGVIDNGRFGTRTIRFETGRLARQAQGSVVAYLDGETMLLSSTSVGKQPKEDCDFFPLTVDVEERSYAAGKIPGSY
FRREGRPSTEAILACRLIDRPLRPSFNAGLRNEVQVIVTVLSIAPGEFYEALAINAASASTLVSGLPFSGPIGGVRLALI
DGQWVAFPRYEDLSGAVFDLTVAGRVFVNESGKEDIAIMMVEAEATEPAWDLIHSHGAKKPNEEVIEEGIESAKVFIRTL
CDIQRDLASKLSLSHTEPDLYPDYSDSVHSFVEGLVKSDLEKVYRGNHDSGYAPNALSASDEIKQKAYDAFHDAVLSGRF
DQDSLSQFPHAYKAVLKDVVRTCVLEGFARMDGRGLSDIRPLDAEVQVVPRVHGSAVFQRGETQVLGVTTLNMLKMEQQI
DSLAPIVSKRYIHHYNFPPYSTGEVGRVGSPKRREIGHGFLAERALVPVLPSREDFPYAIRQVSEALGSNGSTSMGSVCA
STLSLLNAGVPLRAPVAGIAMGLISGRVDGEMRYVTLTDISGSEDALGDMDFKVAGTSDFITALQLDTKLDGIPAHVLSE
ALAHARSARLAILDVLTRVIDSPDQMSEYAPRVVRVKIPVQKIGELIGPKGKVINSIQDETGAEISIEDDGTVYIGSSQA
DSSEKAVAMVNSIVNPVEPCVGSQFLGTVVKNMPFGSFISLVPGKDGLLHISEIRKMVDGRHLESVDEVLSVGQKVLVEV
SKIDDRGKLCLVAVK

Sequences:

>Translated_735_residues
MQFSEGVIDNGRFGTRTIRFETGRLARQAQGSVVAYLDGETMLLSSTSVGKQPKEDCDFFPLTVDVEERSYAAGKIPGSY
FRREGRPSTEAILACRLIDRPLRPSFNAGLRNEVQVIVTVLSIAPGEFYEALAINAASASTLVSGLPFSGPIGGVRLALI
DGQWVAFPRYEDLSGAVFDLTVAGRVFVNESGKEDIAIMMVEAEATEPAWDLIHSHGAKKPNEEVIEEGIESAKVFIRTL
CDIQRDLASKLSLSHTEPDLYPDYSDSVHSFVEGLVKSDLEKVYRGNHDSGYAPNALSASDEIKQKAYDAFHDAVLSGRF
DQDSLSQFPHAYKAVLKDVVRTCVLEGFARMDGRGLSDIRPLDAEVQVVPRVHGSAVFQRGETQVLGVTTLNMLKMEQQI
DSLAPIVSKRYIHHYNFPPYSTGEVGRVGSPKRREIGHGFLAERALVPVLPSREDFPYAIRQVSEALGSNGSTSMGSVCA
STLSLLNAGVPLRAPVAGIAMGLISGRVDGEMRYVTLTDISGSEDALGDMDFKVAGTSDFITALQLDTKLDGIPAHVLSE
ALAHARSARLAILDVLTRVIDSPDQMSEYAPRVVRVKIPVQKIGELIGPKGKVINSIQDETGAEISIEDDGTVYIGSSQA
DSSEKAVAMVNSIVNPVEPCVGSQFLGTVVKNMPFGSFISLVPGKDGLLHISEIRKMVDGRHLESVDEVLSVGQKVLVEV
SKIDDRGKLCLVAVK
>Mature_735_residues
MQFSEGVIDNGRFGTRTIRFETGRLARQAQGSVVAYLDGETMLLSSTSVGKQPKEDCDFFPLTVDVEERSYAAGKIPGSY
FRREGRPSTEAILACRLIDRPLRPSFNAGLRNEVQVIVTVLSIAPGEFYEALAINAASASTLVSGLPFSGPIGGVRLALI
DGQWVAFPRYEDLSGAVFDLTVAGRVFVNESGKEDIAIMMVEAEATEPAWDLIHSHGAKKPNEEVIEEGIESAKVFIRTL
CDIQRDLASKLSLSHTEPDLYPDYSDSVHSFVEGLVKSDLEKVYRGNHDSGYAPNALSASDEIKQKAYDAFHDAVLSGRF
DQDSLSQFPHAYKAVLKDVVRTCVLEGFARMDGRGLSDIRPLDAEVQVVPRVHGSAVFQRGETQVLGVTTLNMLKMEQQI
DSLAPIVSKRYIHHYNFPPYSTGEVGRVGSPKRREIGHGFLAERALVPVLPSREDFPYAIRQVSEALGSNGSTSMGSVCA
STLSLLNAGVPLRAPVAGIAMGLISGRVDGEMRYVTLTDISGSEDALGDMDFKVAGTSDFITALQLDTKLDGIPAHVLSE
ALAHARSARLAILDVLTRVIDSPDQMSEYAPRVVRVKIPVQKIGELIGPKGKVINSIQDETGAEISIEDDGTVYIGSSQA
DSSEKAVAMVNSIVNPVEPCVGSQFLGTVVKNMPFGSFISLVPGKDGLLHISEIRKMVDGRHLESVDEVLSVGQKVLVEV
SKIDDRGKLCLVAVK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=722, Percent_Identity=33.7950138504155, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI145693187, Length=723, Percent_Identity=41.6320885200553, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI115534063, Length=693, Percent_Identity=31.8903318903319, Blast_Score=290, Evalue=3e-78,
Organism=Drosophila melanogaster, GI281362905, Length=724, Percent_Identity=33.1491712707182, Blast_Score=357, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24651641, Length=724, Percent_Identity=33.1491712707182, Blast_Score=357, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24651643, Length=724, Percent_Identity=33.1491712707182, Blast_Score=357, Evalue=2e-98,
Organism=Drosophila melanogaster, GI161079377, Length=696, Percent_Identity=32.4712643678161, Blast_Score=331, Evalue=1e-90,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79496; Mature: 79496

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSEGVIDNGRFGTRTIRFETGRLARQAQGSVVAYLDGETMLLSSTSVGKQPKEDCDFF
CCCCCCCCCCCCCCCEEEEEECCHHHHHCCCCEEEEECCCEEEEECCCCCCCCCCCCCEE
PLTVDVEERSYAAGKIPGSYFRREGRPSTEAILACRLIDRPLRPSFNAGLRNEVQVIVTV
EEEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LSIAPGEFYEALAINAASASTLVSGLPFSGPIGGVRLALIDGQWVAFPRYEDLSGAVFDL
HHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCEEEE
TVAGRVFVNESGKEDIAIMMVEAEATEPAWDLIHSHGAKKPNEEVIEEGIESAKVFIRTL
EEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
CDIQRDLASKLSLSHTEPDLYPDYSDSVHSFVEGLVKSDLEKVYRGNHDSGYAPNALSAS
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
DEIKQKAYDAFHDAVLSGRFDQDSLSQFPHAYKAVLKDVVRTCVLEGFARMDGRGLSDIR
HHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PLDAEVQVVPRVHGSAVFQRGETQVLGVTTLNMLKMEQQIDSLAPIVSKRYIHHYNFPPY
CCCCCEEEECCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
STGEVGRVGSPKRREIGHGFLAERALVPVLPSREDFPYAIRQVSEALGSNGSTSMGSVCA
CCCCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
STLSLLNAGVPLRAPVAGIAMGLISGRVDGEMRYVTLTDISGSEDALGDMDFKVAGTSDF
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCHHH
ITALQLDTKLDGIPAHVLSEALAHARSARLAILDVLTRVIDSPDQMSEYAPRVVRVKIPV
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECH
QKIGELIGPKGKVINSIQDETGAEISIEDDGTVYIGSSQADSSEKAVAMVNSIVNPVEPC
HHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VGSQFLGTVVKNMPFGSFISLVPGKDGLLHISEIRKMVDGRHLESVDEVLSVGQKVLVEV
HCHHHHHHHHHCCCCCCCEEECCCCCCCEEHHHHHHHHCCCHHHHHHHHHHCCHHHHHHH
SKIDDRGKLCLVAVK
HCCCCCCCEEEEEEC
>Mature Secondary Structure
MQFSEGVIDNGRFGTRTIRFETGRLARQAQGSVVAYLDGETMLLSSTSVGKQPKEDCDFF
CCCCCCCCCCCCCCCEEEEEECCHHHHHCCCCEEEEECCCEEEEECCCCCCCCCCCCCEE
PLTVDVEERSYAAGKIPGSYFRREGRPSTEAILACRLIDRPLRPSFNAGLRNEVQVIVTV
EEEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LSIAPGEFYEALAINAASASTLVSGLPFSGPIGGVRLALIDGQWVAFPRYEDLSGAVFDL
HHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCEEEE
TVAGRVFVNESGKEDIAIMMVEAEATEPAWDLIHSHGAKKPNEEVIEEGIESAKVFIRTL
EEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
CDIQRDLASKLSLSHTEPDLYPDYSDSVHSFVEGLVKSDLEKVYRGNHDSGYAPNALSAS
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
DEIKQKAYDAFHDAVLSGRFDQDSLSQFPHAYKAVLKDVVRTCVLEGFARMDGRGLSDIR
HHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PLDAEVQVVPRVHGSAVFQRGETQVLGVTTLNMLKMEQQIDSLAPIVSKRYIHHYNFPPY
CCCCCEEEECCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
STGEVGRVGSPKRREIGHGFLAERALVPVLPSREDFPYAIRQVSEALGSNGSTSMGSVCA
CCCCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
STLSLLNAGVPLRAPVAGIAMGLISGRVDGEMRYVTLTDISGSEDALGDMDFKVAGTSDF
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCHHH
ITALQLDTKLDGIPAHVLSEALAHARSARLAILDVLTRVIDSPDQMSEYAPRVVRVKIPV
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECH
QKIGELIGPKGKVINSIQDETGAEISIEDDGTVYIGSSQADSSEKAVAMVNSIVNPVEPC
HHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VGSQFLGTVVKNMPFGSFISLVPGKDGLLHISEIRKMVDGRHLESVDEVLSVGQKVLVEV
HCHHHHHHHHHCCCCCCCEEECCCCCCCEEHHHHHHHHCCCHHHHHHHHHHCCHHHHHHH
SKIDDRGKLCLVAVK
HCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12902375 [H]