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The map label for this gene is prfA [H]

Identifier: 28572487

GI number: 28572487

Start: 365530

End: 366573

Strand: Direct

Name: prfA [H]

Synonym: TW333

Alternate gene names: 28572487

Gene position: 365530-366573 (Clockwise)

Preceding gene: 28572486

Following gene: 28572488

Centisome position: 39.48

GC content: 46.65

Gene sequence:

>1044_bases
GTGTTTGAGACCATAGATACCCTCTTGCGAGAGCATAGCATCATAGAAGACAGGCTCTCGCGCTGTGACGCAAGTCCGTC
TGAAATGAAAGATTTGAGCTTGAGATATTCGAAGCTATCTCGGGTAAAAGCTGCCTATGAGGAGTTTCAGAAAATATCAC
TTGATCTGAGTGCCGCCCGCGAACTTGCGGATGATGATCCCGGATTTCTCGTAGAAATTCCTGTTCTTACGGAAAAGCTT
TCTGACGCTCAGGCCAGACTGCAAAGCCTTTTAACTCGGAGAGATGAAAACGATCATCGTGATGTGATTATGGAGATAAA
GGGTGGCGAGGGCGGTGCAGAGAGCGCGCTGTTTGCCGCTGACCTATTGAGAATGTATGCGTATTTTGCCGATTCAAACG
GGTGGAAAGTTGAGATACTGAGCTACAGTGAAAACACATTGGGTGGCTACAAAGATGTTCAGCTTGCAATAAGAAAAACA
CCATCAAGCACGCATGGGGTTTGGGAGCTTCTCAAATACGAGGGTGGTGTACACAGGGTACAGAGAGTACCAGCTACAGA
ATCACAGGGCAGAATACATACATCGACAACCGGAGTTCTGGTCTTTCCTGAAGTCGATGCCCCGGGGGAAATCGAAATAA
CTAATGATGAAATCAGAATAGATGTCTTTAGATCATCAGGACCCGGAGGACAGTCTGTTAACACAACAGACTCAGCAGTC
AGAATAACCCATCTCCCAACAGGAATTGTAGTCTCTTGCCAAAACGAGAAAAGCCAGATACAAAACCGAGAATCAGCAAT
GCGGATCCTGCGAGCGAGGCTTATTGCTAAACGCCAGGATGAAATTGAAAAAACTACTCATGCAGCGAGAAAAAGCCAGA
TACGGGCCATGGATCGCTCTGAGAGGATTAGAACCTATAACTTCCCAGAGAATCGCGTGGTTGACCATAGAACGGGCTAT
AAAGCGTATAACCTTGACCAAGTGTTGAATGGTGATGTCTCTCAGCTTATTCGGTCATGTATAGAGGCGGACACGGACGT
TTAG

Upstream 100 bases:

>100_bases
TAGAGTGCGCAGCGAGGGCTCGTGATGTTACCTGTACGATCGGCTTCTAAGACTTTTCTATTGCATATTTTGTCCCATCG
CTGCATTCTTTAGGCTGGTA

Downstream 100 bases:

>100_bases
ATCTTGTTTAGAGATCTTCTGTTGTGGGGCACCCGGCGGCTTTCCAACTCGCTTTCGCCCAGGGTTGAATCTGAAATACT
TCTAAGCTTTGCTTGCAATA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MFETIDTLLREHSIIEDRLSRCDASPSEMKDLSLRYSKLSRVKAAYEEFQKISLDLSAARELADDDPGFLVEIPVLTEKL
SDAQARLQSLLTRRDENDHRDVIMEIKGGEGGAESALFAADLLRMYAYFADSNGWKVEILSYSENTLGGYKDVQLAIRKT
PSSTHGVWELLKYEGGVHRVQRVPATESQGRIHTSTTGVLVFPEVDAPGEIEITNDEIRIDVFRSSGPGGQSVNTTDSAV
RITHLPTGIVVSCQNEKSQIQNRESAMRILRARLIAKRQDEIEKTTHAARKSQIRAMDRSERIRTYNFPENRVVDHRTGY
KAYNLDQVLNGDVSQLIRSCIEADTDV

Sequences:

>Translated_347_residues
MFETIDTLLREHSIIEDRLSRCDASPSEMKDLSLRYSKLSRVKAAYEEFQKISLDLSAARELADDDPGFLVEIPVLTEKL
SDAQARLQSLLTRRDENDHRDVIMEIKGGEGGAESALFAADLLRMYAYFADSNGWKVEILSYSENTLGGYKDVQLAIRKT
PSSTHGVWELLKYEGGVHRVQRVPATESQGRIHTSTTGVLVFPEVDAPGEIEITNDEIRIDVFRSSGPGGQSVNTTDSAV
RITHLPTGIVVSCQNEKSQIQNRESAMRILRARLIAKRQDEIEKTTHAARKSQIRAMDRSERIRTYNFPENRVVDHRTGY
KAYNLDQVLNGDVSQLIRSCIEADTDV
>Mature_347_residues
MFETIDTLLREHSIIEDRLSRCDASPSEMKDLSLRYSKLSRVKAAYEEFQKISLDLSAARELADDDPGFLVEIPVLTEKL
SDAQARLQSLLTRRDENDHRDVIMEIKGGEGGAESALFAADLLRMYAYFADSNGWKVEILSYSENTLGGYKDVQLAIRKT
PSSTHGVWELLKYEGGVHRVQRVPATESQGRIHTSTTGVLVFPEVDAPGEIEITNDEIRIDVFRSSGPGGQSVNTTDSAV
RITHLPTGIVVSCQNEKSQIQNRESAMRILRARLIAKRQDEIEKTTHAARKSQIRAMDRSERIRTYNFPENRVVDHRTGY
KAYNLDQVLNGDVSQLIRSCIEADTDV

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=287, Percent_Identity=42.8571428571429, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI34577120, Length=357, Percent_Identity=35.0140056022409, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=42.1052631578947, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1787462, Length=322, Percent_Identity=44.4099378881988, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI2367172, Length=326, Percent_Identity=37.4233128834356, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=318, Percent_Identity=34.5911949685535, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321295, Length=310, Percent_Identity=40.9677419354839, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI19921226, Length=249, Percent_Identity=43.3734939759036, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39030; Mature: 39030

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETIDTLLREHSIIEDRLSRCDASPSEMKDLSLRYSKLSRVKAAYEEFQKISLDLSAAR
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ELADDDPGFLVEIPVLTEKLSDAQARLQSLLTRRDENDHRDVIMEIKGGEGGAESALFAA
HHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHH
DLLRMYAYFADSNGWKVEILSYSENTLGGYKDVQLAIRKTPSSTHGVWELLKYEGGVHRV
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHEEEEEECCCCCHHHHHHHHHHCCCHHHH
QRVPATESQGRIHTSTTGVLVFPEVDAPGEIEITNDEIRIDVFRSSGPGGQSVNTTDSAV
HCCCCCCCCCEEEECCCCEEEEECCCCCCCEEEECCEEEEEEEECCCCCCCCCCCCCCEE
RITHLPTGIVVSCQNEKSQIQNRESAMRILRARLIAKRQDEIEKTTHAARKSQIRAMDRS
EEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIRTYNFPENRVVDHRTGYKAYNLDQVLNGDVSQLIRSCIEADTDV
CCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFETIDTLLREHSIIEDRLSRCDASPSEMKDLSLRYSKLSRVKAAYEEFQKISLDLSAAR
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ELADDDPGFLVEIPVLTEKLSDAQARLQSLLTRRDENDHRDVIMEIKGGEGGAESALFAA
HHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHH
DLLRMYAYFADSNGWKVEILSYSENTLGGYKDVQLAIRKTPSSTHGVWELLKYEGGVHRV
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHEEEEEECCCCCHHHHHHHHHHCCCHHHH
QRVPATESQGRIHTSTTGVLVFPEVDAPGEIEITNDEIRIDVFRSSGPGGQSVNTTDSAV
HCCCCCCCCCEEEECCCCEEEEECCCCCCCEEEECCEEEEEEEECCCCCCCCCCCCCCEE
RITHLPTGIVVSCQNEKSQIQNRESAMRILRARLIAKRQDEIEKTTHAARKSQIRAMDRS
EEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIRTYNFPENRVVDHRTGYKAYNLDQVLNGDVSQLIRSCIEADTDV
CCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA