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The map label for this gene is rho [H]

Identifier: 28572486

GI number: 28572486

Start: 364090

End: 365454

Strand: Direct

Name: rho [H]

Synonym: TW332

Alternate gene names: 28572486

Gene position: 364090-365454 (Clockwise)

Preceding gene: 28572485

Following gene: 28572487

Centisome position: 39.32

GC content: 49.74

Gene sequence:

>1365_bases
ATGTCTGATGGTGAGGATATTCGTAACCTCAGTCTTTCTAAATTACGCACTCTGGCGATTCAGAGGGGTATTAAGTCTGC
CTACCGGTACAAGAAGAGCGATCTTTTAGAACGCCTGGCCGCCGCTGATAAGTCGGTTGTGGATGCCGCTGATTCGCCCG
GAGAGAGTCCTATAACACTTGATGATCTGGCGAACGCTATACCGGAAGGTACGCCAGAGAGGCAGCAAACAGGCGATATC
CGGCGTGTTAATGAGCCTGATCGGGTTAGCATAACTCGTGAGCGCCGTGGCAGGAGTCGGCGACGGCGGATCGAAGAAGG
GATTTTAGACTCTTCACAGCTCGTTCCGATTGCAGGGGTTCTTGATGTACTGGATAGTTGTGCCTTTATACGAACTACCG
GGTATTCTCCGGGGGATTCTGACATATATGTCTCACTGAGTCAGGTAAAAAAATATTCGCTCCGTAAAGGTGATGCAGTT
GCCGGCGAGGTTGCCCGGGTAAAAAGTATTGATAGTTACAAACAGAGGCATTTTCCGCTTGTTAGTGTGAACACAGTAAA
TGGTTTAGAATTTCAGCAGGTAACTACTCGTCCGGCCTTTGAATCAGGTATGTGCGTGCCCCCTACGCGTCGCCTTAGAA
TAACACCAAAGACGGCCCCGGTCGAAAAGAGGATAGTTGATCTGGTATCGCCGGTGGCCAAAGGAACACGTGGCCTTGTT
CGTGGACCAAGTAAAAGCGGTCGCACAACTGTTCTGCACGAAATCGCTGCCGGCCTGTGTGAGCAAAATAAAGAACTTCA
TCTGATTACTCTCCTTGTTGGTATAAGGCCCGAAGAGGCAACTCACATGTCCCGTACTTTGAGAGGTGAAGTGGTTGTAT
CCAGCATGGAATGCTCGCCCGAAGAACAAGTTGGCGTTGCAGAATTGGTTCTTGAGCGTTCTAAGCGTCTTGTTGAACAG
GGTAGGGATGTTCTTCTTATTGTCGATTCGATGACCCATCTTGGTCGCGCCTATGCTCTTGTTGGTGCGCCACCAAGTCG
CCTGATTGGATCCGCAGCGGAATTAAGTGCTTTGCGCCCGATCACAAGCTTTTTCCTTTCCGCACGCTGTATTGAAAATA
GCGGCTCGATAACAATGCTTGCCACAGTTCTTAGTGAAAGCGAGAGTGCGTTTGACCGATTGGTTGAATATGATCTTGTT
GCTGTGAGCGGCATGCAGCTTGTTTTGGATAAACTGGCCTCTGAGCGCCGAGATTTTCCAGCCATTAACCCCATACGGAG
TTCTAGTAGGGATGAATTTACTCTCCTGAGTGCTTCTGAACAGAGTGAGGTGAAAAGGCTTAGAGTGCGCAGCGAGGGCT
CGTGA

Upstream 100 bases:

>100_bases
GGGCATGACCCTCTAGGACCCTTCTCTTGAGGCTTCGCCCGTGCGGTGTATTCTTCTGTTTTGATAATATTTTGGACTGT
CTGGTCCTTGAAGGGAAACC

Downstream 100 bases:

>100_bases
TGTTACCTGTACGATCGGCTTCTAAGACTTTTCTATTGCATATTTTGTCCCATCGCTGCATTCTTTAGGCTGGTAGTGTT
TGAGACCATAGATACCCTCT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSDGEDIRNLSLSKLRTLAIQRGIKSAYRYKKSDLLERLAAADKSVVDAADSPGESPITLDDLANAIPEGTPERQQTGDI
RRVNEPDRVSITRERRGRSRRRRIEEGILDSSQLVPIAGVLDVLDSCAFIRTTGYSPGDSDIYVSLSQVKKYSLRKGDAV
AGEVARVKSIDSYKQRHFPLVSVNTVNGLEFQQVTTRPAFESGMCVPPTRRLRITPKTAPVEKRIVDLVSPVAKGTRGLV
RGPSKSGRTTVLHEIAAGLCEQNKELHLITLLVGIRPEEATHMSRTLRGEVVVSSMECSPEEQVGVAELVLERSKRLVEQ
GRDVLLIVDSMTHLGRAYALVGAPPSRLIGSAAELSALRPITSFFLSARCIENSGSITMLATVLSESESAFDRLVEYDLV
AVSGMQLVLDKLASERRDFPAINPIRSSSRDEFTLLSASEQSEVKRLRVRSEGS

Sequences:

>Translated_454_residues
MSDGEDIRNLSLSKLRTLAIQRGIKSAYRYKKSDLLERLAAADKSVVDAADSPGESPITLDDLANAIPEGTPERQQTGDI
RRVNEPDRVSITRERRGRSRRRRIEEGILDSSQLVPIAGVLDVLDSCAFIRTTGYSPGDSDIYVSLSQVKKYSLRKGDAV
AGEVARVKSIDSYKQRHFPLVSVNTVNGLEFQQVTTRPAFESGMCVPPTRRLRITPKTAPVEKRIVDLVSPVAKGTRGLV
RGPSKSGRTTVLHEIAAGLCEQNKELHLITLLVGIRPEEATHMSRTLRGEVVVSSMECSPEEQVGVAELVLERSKRLVEQ
GRDVLLIVDSMTHLGRAYALVGAPPSRLIGSAAELSALRPITSFFLSARCIENSGSITMLATVLSESESAFDRLVEYDLV
AVSGMQLVLDKLASERRDFPAINPIRSSSRDEFTLLSASEQSEVKRLRVRSEGS
>Mature_453_residues
SDGEDIRNLSLSKLRTLAIQRGIKSAYRYKKSDLLERLAAADKSVVDAADSPGESPITLDDLANAIPEGTPERQQTGDIR
RVNEPDRVSITRERRGRSRRRRIEEGILDSSQLVPIAGVLDVLDSCAFIRTTGYSPGDSDIYVSLSQVKKYSLRKGDAVA
GEVARVKSIDSYKQRHFPLVSVNTVNGLEFQQVTTRPAFESGMCVPPTRRLRITPKTAPVEKRIVDLVSPVAKGTRGLVR
GPSKSGRTTVLHEIAAGLCEQNKELHLITLLVGIRPEEATHMSRTLRGEVVVSSMECSPEEQVGVAELVLERSKRLVEQG
RDVLLIVDSMTHLGRAYALVGAPPSRLIGSAAELSALRPITSFFLSARCIENSGSITMLATVLSESESAFDRLVEYDLVA
VSGMQLVLDKLASERRDFPAINPIRSSSRDEFTLLSASEQSEVKRLRVRSEGS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=336, Percent_Identity=35.4166666666667, Blast_Score=204, Evalue=8e-54,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 49791; Mature: 49660

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGEDIRNLSLSKLRTLAIQRGIKSAYRYKKSDLLERLAAADKSVVDAADSPGESPITL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEH
DDLANAIPEGTPERQQTGDIRRVNEPDRVSITRERRGRSRRRRIEEGILDSSQLVPIAGV
HHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEHHHHCHHHHHHHHHCCCCCCCCCHHHHH
LDVLDSCAFIRTTGYSPGDSDIYVSLSQVKKYSLRKGDAVAGEVARVKSIDSYKQRHFPL
HHHHHCCCEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCE
VSVNTVNGLEFQQVTTRPAFESGMCVPPTRRLRITPKTAPVEKRIVDLVSPVAKGTRGLV
EEEECCCCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCC
RGPSKSGRTTVLHEIAAGLCEQNKELHLITLLVGIRPEEATHMSRTLRGEVVVSSMECSP
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHEEEEECCCCC
EEQVGVAELVLERSKRLVEQGRDVLLIVDSMTHLGRAYALVGAPPSRLIGSAAELSALRP
HHHCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHEEEECCCHHHHHCCHHHHHHHHH
ITSFFLSARCIENSGSITMLATVLSESESAFDRLVEYDLVAVSGMQLVLDKLASERRDFP
HHHHHHHHHHHCCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AINPIRSSSRDEFTLLSASEQSEVKRLRVRSEGS
CCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDGEDIRNLSLSKLRTLAIQRGIKSAYRYKKSDLLERLAAADKSVVDAADSPGESPITL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEH
DDLANAIPEGTPERQQTGDIRRVNEPDRVSITRERRGRSRRRRIEEGILDSSQLVPIAGV
HHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEHHHHCHHHHHHHHHCCCCCCCCCHHHHH
LDVLDSCAFIRTTGYSPGDSDIYVSLSQVKKYSLRKGDAVAGEVARVKSIDSYKQRHFPL
HHHHHCCCEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCE
VSVNTVNGLEFQQVTTRPAFESGMCVPPTRRLRITPKTAPVEKRIVDLVSPVAKGTRGLV
EEEECCCCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCC
RGPSKSGRTTVLHEIAAGLCEQNKELHLITLLVGIRPEEATHMSRTLRGEVVVSSMECSP
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHEEEEECCCCC
EEQVGVAELVLERSKRLVEQGRDVLLIVDSMTHLGRAYALVGAPPSRLIGSAAELSALRP
HHHCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHEEEECCCHHHHHCCHHHHHHHHH
ITSFFLSARCIENSGSITMLATVLSESESAFDRLVEYDLVAVSGMQLVLDKLASERRDFP
HHHHHHHHHHHCCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AINPIRSSSRDEFTLLSASEQSEVKRLRVRSEGS
CCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8557681; 8051015 [H]