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The map label for this gene is fgs [H]

Identifier: 28572444

GI number: 28572444

Start: 327361

End: 328722

Strand: Direct

Name: fgs [H]

Synonym: TW288

Alternate gene names: 28572444

Gene position: 327361-328722 (Clockwise)

Preceding gene: 28572443

Following gene: 28572445

Centisome position: 35.35

GC content: 45.01

Gene sequence:

>1362_bases
ATGTCAAGAGCCGGCGAGGCCCTCCCGCAGCCGCGGCTTGATGGCACCAAACTTGCCCTATCGTATCTGTCAAATCCTCA
AGAATGTGCTCCGGTAATTCACATCACCGGAACAAATGGGAAAACAACTGTAAGCAGGATGATAGCCTGCATCCTAACAT
CTCTCGGGCTGCGTGTCGGTTTATTTACTAGCCCTCACATTACCGCTCTCGAGGAGAGAATTCAGATTGACATGAATCCC
GTCCAGGGGCACATCTTAGAGAAACACTGGTTCGATATTCAGTTTCAGTTGCGGCTCGCGGATGCTCATCTTGAGAAAAA
AAACTGCCCACCGCTGACATTTTTTGAGTGTATGACGGTTCTTGCATTTTCATGCTTTGCAGATACACCTGTGGATGTCA
TGGTGCTTGAGGTTGGTATGGGTGGTGCTTGGGATGCAACCAATGTTGTCGATGCCAGGGTGTGTGTTTTTACGCCAATT
GATTTTGATCATACAGGTCGTTTGGGAAGCAGGTTAGAAGAGATTGCACACACAAAAGCTGGGATAATCAAGCCAGGATC
TTTTGTTGTAGCTGCAGAACAACATCAAGTTGTGCAAGAGTGCCTTGAGGATTATTTTGCATCCATAAATATCGGATGCC
AGAGCTATGACACAGAATTACCTGACAAAATAAGGTTCTTTGGGCAAGATTTTGACTGTGATTACAAACCCGTTGCGGGA
GGGCAAATACTCGATTTGCGTGGTCTATACGGTGAGTGCAACGAGGTTTTTCTTCCACTTTTTGGTGGATATCAGGCTAA
AAACGCTGCCCTGTCCTTAGCTGCCGTGGAAAGCTTTTTTGGTAGGGTGGAACATGATATAGTCCTCGAGGCCTTTTCAA
ATATAACGAGCCCAGGGCGGCTTCAGATTATTTCTAAAAATCCAGTTGTTTTACTTGATGCAGCACATAATCCACATGCG
GCAAAACATCTCGCAATTGCTCTGGATGAATTTTTTTCCTTTGGGAAAATTGTGTTTATTTTAGCGTTTTTATCCGATAA
AGACATTGAAGGCATAATTCGTAATCTAATGTCTTTTTTACACGGCTCGCTGCGTGTAACGAGTGACTCCGTAGGTTACA
GTGAATCGGAAAGCACCCGGTTCAGTAACCCTCGTGTAACATTTGTCATAACACAATCAACACACCCGCGAGCGGTTGCC
GCTGATGTAGCGTTTAGCATTGCTAAAAGAGTTGTAGCTAACGAGGGACTTATAGTATGTGAAAGTGCGAATGATGCGTT
TCAGTACAGTACCAGGCTTAATCCGGATGCCATTGTTGTCTCCGGCTCTATCAGCATTCTTAGTAGTTTTATTCCGCGAT
AA

Upstream 100 bases:

>100_bases
ACATCTATTCGGCTCGATAGCCTATTCAGTTTGTCCGCCTGAGTTTCCCTTACTGGATGATTGGAGCCTGTAGGCCGTGT
CAGAGTTTTACAGGCAGATT

Downstream 100 bases:

>100_bases
ATAAATGAATTATCTCAGGACAAAGGTTGCAAGAGTTGTTCTCTCTTTTGAGCTCGTTGCATTTATTCTCGCAGGTGTTT
TTTTGTTTACTATGCCATTT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSRAGEALPQPRLDGTKLALSYLSNPQECAPVIHITGTNGKTTVSRMIACILTSLGLRVGLFTSPHITALEERIQIDMNP
VQGHILEKHWFDIQFQLRLADAHLEKKNCPPLTFFECMTVLAFSCFADTPVDVMVLEVGMGGAWDATNVVDARVCVFTPI
DFDHTGRLGSRLEEIAHTKAGIIKPGSFVVAAEQHQVVQECLEDYFASINIGCQSYDTELPDKIRFFGQDFDCDYKPVAG
GQILDLRGLYGECNEVFLPLFGGYQAKNAALSLAAVESFFGRVEHDIVLEAFSNITSPGRLQIISKNPVVLLDAAHNPHA
AKHLAIALDEFFSFGKIVFILAFLSDKDIEGIIRNLMSFLHGSLRVTSDSVGYSESESTRFSNPRVTFVITQSTHPRAVA
ADVAFSIAKRVVANEGLIVCESANDAFQYSTRLNPDAIVVSGSISILSSFIPR

Sequences:

>Translated_453_residues
MSRAGEALPQPRLDGTKLALSYLSNPQECAPVIHITGTNGKTTVSRMIACILTSLGLRVGLFTSPHITALEERIQIDMNP
VQGHILEKHWFDIQFQLRLADAHLEKKNCPPLTFFECMTVLAFSCFADTPVDVMVLEVGMGGAWDATNVVDARVCVFTPI
DFDHTGRLGSRLEEIAHTKAGIIKPGSFVVAAEQHQVVQECLEDYFASINIGCQSYDTELPDKIRFFGQDFDCDYKPVAG
GQILDLRGLYGECNEVFLPLFGGYQAKNAALSLAAVESFFGRVEHDIVLEAFSNITSPGRLQIISKNPVVLLDAAHNPHA
AKHLAIALDEFFSFGKIVFILAFLSDKDIEGIIRNLMSFLHGSLRVTSDSVGYSESESTRFSNPRVTFVITQSTHPRAVA
ADVAFSIAKRVVANEGLIVCESANDAFQYSTRLNPDAIVVSGSISILSSFIPR
>Mature_452_residues
SRAGEALPQPRLDGTKLALSYLSNPQECAPVIHITGTNGKTTVSRMIACILTSLGLRVGLFTSPHITALEERIQIDMNPV
QGHILEKHWFDIQFQLRLADAHLEKKNCPPLTFFECMTVLAFSCFADTPVDVMVLEVGMGGAWDATNVVDARVCVFTPID
FDHTGRLGSRLEEIAHTKAGIIKPGSFVVAAEQHQVVQECLEDYFASINIGCQSYDTELPDKIRFFGQDFDCDYKPVAGG
QILDLRGLYGECNEVFLPLFGGYQAKNAALSLAAVESFFGRVEHDIVLEAFSNITSPGRLQIISKNPVVLLDAAHNPHAA
KHLAIALDEFFSFGKIVFILAFLSDKDIEGIIRNLMSFLHGSLRVTSDSVGYSESESTRFSNPRVTFVITQSTHPRAVAA
DVAFSIAKRVVANEGLIVCESANDAFQYSTRLNPDAIVVSGSISILSSFIPR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=364, Percent_Identity=28.2967032967033, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI66932990, Length=364, Percent_Identity=28.2967032967033, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788654, Length=346, Percent_Identity=31.7919075144509, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553148, Length=409, Percent_Identity=27.3838630806846, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=409, Percent_Identity=27.3838630806846, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=409, Percent_Identity=27.3838630806846, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=358, Percent_Identity=28.7709497206704, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324815, Length=358, Percent_Identity=26.2569832402235, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24641571, Length=175, Percent_Identity=41.7142857142857, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581568, Length=157, Percent_Identity=33.7579617834395, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49590; Mature: 49459

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAGEALPQPRLDGTKLALSYLSNPQECAPVIHITGTNGKTTVSRMIACILTSLGLRVG
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEE
LFTSPHITALEERIQIDMNPVQGHILEKHWFDIQFQLRLADAHLEKKNCPPLTFFECMTV
EECCCCHHHHHHHHEEECCCCCCCEECCEEEEEEEEEEEECHHHCCCCCCCCHHHHHHHH
LAFSCFADTPVDVMVLEVGMGGAWDATNVVDARVCVFTPIDFDHTGRLGSRLEEIAHTKA
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
GIIKPGSFVVAAEQHQVVQECLEDYFASINIGCQSYDTELPDKIRFFGQDFDCDYKPVAG
CCCCCCCEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
GQILDLRGLYGECNEVFLPLFGGYQAKNAALSLAAVESFFGRVEHDIVLEAFSNITSPGR
CEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LQIISKNPVVLLDAAHNPHAAKHLAIALDEFFSFGKIVFILAFLSDKDIEGIIRNLMSFL
EEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HGSLRVTSDSVGYSESESTRFSNPRVTFVITQSTHPRAVAADVAFSIAKRVVANEGLIVC
CCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEE
ESANDAFQYSTRLNPDAIVVSGSISILSSFIPR
ECCCCCEEEECCCCCCEEEEECHHHHHHHHCCC
>Mature Secondary Structure 
SRAGEALPQPRLDGTKLALSYLSNPQECAPVIHITGTNGKTTVSRMIACILTSLGLRVG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEE
LFTSPHITALEERIQIDMNPVQGHILEKHWFDIQFQLRLADAHLEKKNCPPLTFFECMTV
EECCCCHHHHHHHHEEECCCCCCCEECCEEEEEEEEEEEECHHHCCCCCCCCHHHHHHHH
LAFSCFADTPVDVMVLEVGMGGAWDATNVVDARVCVFTPIDFDHTGRLGSRLEEIAHTKA
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
GIIKPGSFVVAAEQHQVVQECLEDYFASINIGCQSYDTELPDKIRFFGQDFDCDYKPVAG
CCCCCCCEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
GQILDLRGLYGECNEVFLPLFGGYQAKNAALSLAAVESFFGRVEHDIVLEAFSNITSPGR
CEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LQIISKNPVVLLDAAHNPHAAKHLAIALDEFFSFGKIVFILAFLSDKDIEGIIRNLMSFL
EEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HGSLRVTSDSVGYSESESTRFSNPRVTFVITQSTHPRAVAADVAFSIAKRVVANEGLIVC
CCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEE
ESANDAFQYSTRLNPDAIVVSGSISILSSFIPR
ECCCCCEEEECCCCCCEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]