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The map label for this gene is truA

Identifier: 28572394

GI number: 28572394

Start: 258575

End: 259378

Strand: Direct

Name: truA

Synonym: TW234

Alternate gene names: 28572394

Gene position: 258575-259378 (Clockwise)

Preceding gene: 28572393

Following gene: 28572399

Centisome position: 27.93

GC content: 46.39

Gene sequence:

>804_bases
GTGAATAAGACCCGATATCGTCTTGATGTTGCATATGATGGAGCGAATTTTTGTGGTTGGGCACCGCAGCCCGGGCTTAG
GACCGTCGGTGGTCTCATTCTTGATGCTTTACGCCTTATCTGCAGCGAACCGCCCGACATTGTCATAGCGGCAAGAACAG
ATGCTGGTGTTCACGCCCTACAACAAGTGTGTCATGTTGACCTTATTTCATCACCAGACCCTGTTTGGCTTTTGCACAGA
CTCCGCTCTCTACTGAAATCCGAGACCGACCTTCATATCCTTTCTGCTGTCAAGGCACCGGTGAATTTTCACGCTCGTTT
CTCAGCAATAGGCAGAAGATATGTTTACCGTGTCATTGACAAGCGCTCTTCGTGGTATCCCCAGAATAGATATTTTGTTT
ACAGAGTAAATGCTTTTTTGCAAGATTATCGGATGCGCAGGGCAGCTTCTGGCCTTATTGGGCTAAAGGATTTTGGTGCT
TTTTGCAAGCCGCGTCGCATGGGAAGTACAGTACGTCATCTAAGGCAATTTGAAGTTATTAGGCAGCCAGATGGACAGAT
ACATTTCTTTCTCGAATCAGATGCTTTTTGTCACTCTATGGTCAGAAACTTAGTGGGCAGCTTGATAGAAGTGGGTCGTG
GGGCACTTACATTACAAGATCTGTTTTGCTATACCAAAATTGCGAAGAGAACGCCAAAAATTCCTACCCTGCCGCCCCAT
GCACTCACTTTGATTGGCATTGACTATCCGCAGGAACATCTTTTTGAGTGTCAAAACAGAAAAACCCGACAGAAGCGCAC
TTGA

Upstream 100 bases:

>100_bases
GGTGATTTGCCTGATGCCGATACTGCACCCGCGGACCCTGGCGAATCTTCTTCTAACCAGAGAGTCATTCGGTAATCCGC
GTAAGTGCCGACTTATTCTA

Downstream 100 bases:

>100_bases
AGTTATCAAGACGACTACTACGGGGCAAGTTTGTTTTTCCGCAAGGAATGAATACACACTGATCGGTTTATCTGCAGTTT
TCAAACCTGTCGAATACTTG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNKTRYRLDVAYDGANFCGWAPQPGLRTVGGLILDALRLICSEPPDIVIAARTDAGVHALQQVCHVDLISSPDPVWLLHR
LRSLLKSETDLHILSAVKAPVNFHARFSAIGRRYVYRVIDKRSSWYPQNRYFVYRVNAFLQDYRMRRAASGLIGLKDFGA
FCKPRRMGSTVRHLRQFEVIRQPDGQIHFFLESDAFCHSMVRNLVGSLIEVGRGALTLQDLFCYTKIAKRTPKIPTLPPH
ALTLIGIDYPQEHLFECQNRKTRQKRT

Sequences:

>Translated_267_residues
MNKTRYRLDVAYDGANFCGWAPQPGLRTVGGLILDALRLICSEPPDIVIAARTDAGVHALQQVCHVDLISSPDPVWLLHR
LRSLLKSETDLHILSAVKAPVNFHARFSAIGRRYVYRVIDKRSSWYPQNRYFVYRVNAFLQDYRMRRAASGLIGLKDFGA
FCKPRRMGSTVRHLRQFEVIRQPDGQIHFFLESDAFCHSMVRNLVGSLIEVGRGALTLQDLFCYTKIAKRTPKIPTLPPH
ALTLIGIDYPQEHLFECQNRKTRQKRT
>Mature_267_residues
MNKTRYRLDVAYDGANFCGWAPQPGLRTVGGLILDALRLICSEPPDIVIAARTDAGVHALQQVCHVDLISSPDPVWLLHR
LRSLLKSETDLHILSAVKAPVNFHARFSAIGRRYVYRVIDKRSSWYPQNRYFVYRVNAFLQDYRMRRAASGLIGLKDFGA
FCKPRRMGSTVRHLRQFEVIRQPDGQIHFFLESDAFCHSMVRNLVGSLIEVGRGALTLQDLFCYTKIAKRTPKIPTLPPH
ALTLIGIDYPQEHLFECQNRKTRQKRT

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=239, Percent_Identity=30.5439330543933, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=31.4741035856574, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_TROW8 (Q820Z2)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789174.1
- ProteinModelPortal:   Q820Z2
- SMR:   Q820Z2
- STRING:   Q820Z2
- GeneID:   1064526
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW234
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q820Z2
- ProtClustDB:   PRK00021
- BioCyc:   TWHI218496:TW0219-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30590; Mature: 30590

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTRYRLDVAYDGANFCGWAPQPGLRTVGGLILDALRLICSEPPDIVIAARTDAGVHAL
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
QQVCHVDLISSPDPVWLLHRLRSLLKSETDLHILSAVKAPVNFHARFSAIGRRYVYRVID
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KRSSWYPQNRYFVYRVNAFLQDYRMRRAASGLIGLKDFGAFCKPRRMGSTVRHLRQFEVI
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
RQPDGQIHFFLESDAFCHSMVRNLVGSLIEVGRGALTLQDLFCYTKIAKRTPKIPTLPPH
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
ALTLIGIDYPQEHLFECQNRKTRQKRT
EEEEEECCCCHHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MNKTRYRLDVAYDGANFCGWAPQPGLRTVGGLILDALRLICSEPPDIVIAARTDAGVHAL
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
QQVCHVDLISSPDPVWLLHRLRSLLKSETDLHILSAVKAPVNFHARFSAIGRRYVYRVID
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KRSSWYPQNRYFVYRVNAFLQDYRMRRAASGLIGLKDFGAFCKPRRMGSTVRHLRQFEVI
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
RQPDGQIHFFLESDAFCHSMVRNLVGSLIEVGRGALTLQDLFCYTKIAKRTPKIPTLPPH
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
ALTLIGIDYPQEHLFECQNRKTRQKRT
EEEEEECCCCHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174