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The map label for this gene is braE [H]

Identifier: 28572362

GI number: 28572362

Start: 240175

End: 241269

Strand: Direct

Name: braE [H]

Synonym: TW202

Alternate gene names: 28572362

Gene position: 240175-241269 (Clockwise)

Preceding gene: 28572361

Following gene: 28572363

Centisome position: 25.94

GC content: 46.39

Gene sequence:

>1095_bases
GTGATGGGCTTTCTATCGTCTGTTATGCGGTGGTGGAAGGGTATGTCTCGCCCCCAGCAGTGGTTTTTTCTTCTTCCGTT
TGTGGTCCTTGTTTATCTTTTGCCAATTATTAATCCTCCGGTTATCACAACCGAACCGGGCAATAATTTTCCAATCTCAC
TGTTTACAATGGCAATTTATGCCCTCGCCGCGGTCGGTCTTAATGTAGTTGTTGGTTATGCAGGACTTCTTGATTTGGGT
TATATTGCATTTTTTGCGGTTGGTGCTTATGTATCTGCTGTATTTACGAGTCCGGATTCTCCGTATGTCAAAATTCCTTA
TCTTTGGACCATTCCTGTTGCGATTTTTACTGCCATGGTTTTTGGCGCAGCCCTGGGTGTACCTACCCTCCGTCTTCGGG
GAGACTATCTGGCGATTGTTACTCTTGGGTTTGGAGAGATAGTCAGAATTATGGCGACTGTTATCCCTGCCCTCCGTGGT
AACCTCGGGTTTTCCAATGTTGGCCACCCGCCGGGCGACTATCCTTCTGGCCAGCCGATTTTCACGCCTGATAATGGAGT
AGCTTGGTATTGGGTCGCTATTACAGTTGTTATATTCGTTCTTGTAATCGTTGGGAATCTCGAGAGGAGTATTGTCGGCA
GAAACTGGTTTGCTATCTGCCAGGATGAAGATGCTGCAGAGGTTATGGGTGTGAATACCTTTTCCTTCAAGGTATGGGCT
TTTGCAATTGGTGCTGCAATTGGTGGCCTCTCCGGTTCCCTGCAGGCTGCACAGACAGGGTTTGTAAATAATCAGCGTTT
TGATGTTGCGACCTCCGTTTTATTCCTTGTTGCAGTCGTACTTGGCGGTTCTGGTAACAAATTTGGTGCTGCAATTGGTG
GTGCACTTGTGGCGTATATTCCACTGCGTTTTACGGAGATTGCACAATACAAGTATTTGATCTTCGGTGTATGTCTTATA
CTTCTGATGCTTTTTAGACATGAAGGCCTGTTTCCTATGAAGGCAAATCTTTTTTCAAGACAGGTTTTGCTGCGGAAATT
GTCATTGCGCCATTCACCCGATAAACGTGCGTCATCTTCGCCCTCGGGGTCTTGA

Upstream 100 bases:

>100_bases
AGTGGGGTGATGTTTCTGTCTTTGTTCTTCTGTTACTAGTTTTACTTTTCAGGCCATCTGGTATCCTCGGCAGATCTGGC
CTTTCGGCTAAAAGCAGGAT

Downstream 100 bases:

>100_bases
TATGCAAGACGCGCTGTTACAGTCTGAGCATCTTCTTGATGTGCGTGATCTCGTTGTTCGCTTTGGGGGTATTGTCGCGC
TCGACGGCGTCTCGTTCAGT

Product: ABC transporter branched chain amino acid transport permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MMGFLSSVMRWWKGMSRPQQWFFLLPFVVLVYLLPIINPPVITTEPGNNFPISLFTMAIYALAAVGLNVVVGYAGLLDLG
YIAFFAVGAYVSAVFTSPDSPYVKIPYLWTIPVAIFTAMVFGAALGVPTLRLRGDYLAIVTLGFGEIVRIMATVIPALRG
NLGFSNVGHPPGDYPSGQPIFTPDNGVAWYWVAITVVIFVLVIVGNLERSIVGRNWFAICQDEDAAEVMGVNTFSFKVWA
FAIGAAIGGLSGSLQAAQTGFVNNQRFDVATSVLFLVAVVLGGSGNKFGAAIGGALVAYIPLRFTEIAQYKYLIFGVCLI
LLMLFRHEGLFPMKANLFSRQVLLRKLSLRHSPDKRASSSPSGS

Sequences:

>Translated_364_residues
MMGFLSSVMRWWKGMSRPQQWFFLLPFVVLVYLLPIINPPVITTEPGNNFPISLFTMAIYALAAVGLNVVVGYAGLLDLG
YIAFFAVGAYVSAVFTSPDSPYVKIPYLWTIPVAIFTAMVFGAALGVPTLRLRGDYLAIVTLGFGEIVRIMATVIPALRG
NLGFSNVGHPPGDYPSGQPIFTPDNGVAWYWVAITVVIFVLVIVGNLERSIVGRNWFAICQDEDAAEVMGVNTFSFKVWA
FAIGAAIGGLSGSLQAAQTGFVNNQRFDVATSVLFLVAVVLGGSGNKFGAAIGGALVAYIPLRFTEIAQYKYLIFGVCLI
LLMLFRHEGLFPMKANLFSRQVLLRKLSLRHSPDKRASSSPSGS
>Mature_364_residues
MMGFLSSVMRWWKGMSRPQQWFFLLPFVVLVYLLPIINPPVITTEPGNNFPISLFTMAIYALAAVGLNVVVGYAGLLDLG
YIAFFAVGAYVSAVFTSPDSPYVKIPYLWTIPVAIFTAMVFGAALGVPTLRLRGDYLAIVTLGFGEIVRIMATVIPALRG
NLGFSNVGHPPGDYPSGQPIFTPDNGVAWYWVAITVVIFVLVIVGNLERSIVGRNWFAICQDEDAAEVMGVNTFSFKVWA
FAIGAAIGGLSGSLQAAQTGFVNNQRFDVATSVLFLVAVVLGGSGNKFGAAIGGALVAYIPLRFTEIAQYKYLIFGVCLI
LLMLFRHEGLFPMKANLFSRQVLLRKLSLRHSPDKRASSSPSGS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=300, Percent_Identity=37.6666666666667, Blast_Score=145, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39441; Mature: 39441

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGFLSSVMRWWKGMSRPQQWFFLLPFVVLVYLLPIINPPVITTEPGNNFPISLFTMAIY
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
ALAAVGLNVVVGYAGLLDLGYIAFFAVGAYVSAVFTSPDSPYVKIPYLWTIPVAIFTAMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
FGAALGVPTLRLRGDYLAIVTLGFGEIVRIMATVIPALRGNLGFSNVGHPPGDYPSGQPI
HHHHHCCCEEEECCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
FTPDNGVAWYWVAITVVIFVLVIVGNLERSIVGRNWFAICQDEDAAEVMGVNTFSFKVWA
ECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEECCCCHHHHHCCCHHHHHHHH
FAIGAAIGGLSGSLQAAQTGFVNNQRFDVATSVLFLVAVVLGGSGNKFGAAIGGALVAYI
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
PLRFTEIAQYKYLIFGVCLILLMLFRHEGLFPMKANLFSRQVLLRKLSLRHSPDKRASSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PSGS
CCCC
>Mature Secondary Structure
MMGFLSSVMRWWKGMSRPQQWFFLLPFVVLVYLLPIINPPVITTEPGNNFPISLFTMAIY
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
ALAAVGLNVVVGYAGLLDLGYIAFFAVGAYVSAVFTSPDSPYVKIPYLWTIPVAIFTAMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
FGAALGVPTLRLRGDYLAIVTLGFGEIVRIMATVIPALRGNLGFSNVGHPPGDYPSGQPI
HHHHHCCCEEEECCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
FTPDNGVAWYWVAITVVIFVLVIVGNLERSIVGRNWFAICQDEDAAEVMGVNTFSFKVWA
ECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEECCCCHHHHHCCCHHHHHHHH
FAIGAAIGGLSGSLQAAQTGFVNNQRFDVATSVLFLVAVVLGGSGNKFGAAIGGALVAYI
HHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
PLRFTEIAQYKYLIFGVCLILLMLFRHEGLFPMKANLFSRQVLLRKLSLRHSPDKRASSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PSGS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]