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The map label for this gene is folE [H]

Identifier: 28572336

GI number: 28572336

Start: 216002

End: 216547

Strand: Direct

Name: folE [H]

Synonym: TW174

Alternate gene names: 28572336

Gene position: 216002-216547 (Clockwise)

Preceding gene: 28572335

Following gene: 28572337

Centisome position: 23.33

GC content: 49.82

Gene sequence:

>546_bases
GTGACGAAGTTGTGTAGATTTGAGAGTGGTTCGGCGCAATCCGCGGTGGCGAATTTGCTGTCCGCGCTTGGGCAGGACGT
AGGCAGGCTAAAGGAGACTCCTTCTATTGCATCCTCTGGCCTTGAGTCTCTTATCTCTGGTAGTGAAACGAATCCAGCAG
ATTGCCTTGTTGACCTAATTCCCAACACACAAAAGGGGATTGTGATTTTGCGCGATATACCATTCAGATCGATCTGTGAA
CATCACTTATTACCATTTGTGGGCACGGCGAATCTGCTGTATGAACCAGGGAAGTTTCTTGCAGGCCTAGGTAATCTCAC
TTCCCTGGTGCGATGTGCTGCGCGGCGCCTTAGCATTCAGGAGCGCGTAACAGATCAAATAGCTGATGCGATTTATACGG
GCCTTTGTGCGCGCCGCGTTCTTGTGTCACTTGCTGCCCGGCATACCTGCATTTTGGATGAAGCTGGAAATACAGAAGTT
ACGACCTTCGCGCATCGGGGTATAGGAGATGCCGATATCATCTCTTTTATGACAATAATCCGGTAG

Upstream 100 bases:

>100_bases
ACCGCAAAATCGCCAAAGAGAACTTCCAACAGACGTAGGTTGCCTTCAGCAAGGCGCCCGAGGAAGGATTCGGAGACTCA
GGAATAAGTTCAGCCATTCG

Downstream 100 bases:

>100_bases
CGTGTACCTGCTATTTCGCTTGGGTTAACGTGTATTGTGATGAACATGAGCTATACTCCCGACCCATTTCCACCTCCTAA
TGTAATGGGCATTCTGAATA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MTKLCRFESGSAQSAVANLLSALGQDVGRLKETPSIASSGLESLISGSETNPADCLVDLIPNTQKGIVILRDIPFRSICE
HHLLPFVGTANLLYEPGKFLAGLGNLTSLVRCAARRLSIQERVTDQIADAIYTGLCARRVLVSLAARHTCILDEAGNTEV
TTFAHRGIGDADIISFMTIIR

Sequences:

>Translated_181_residues
MTKLCRFESGSAQSAVANLLSALGQDVGRLKETPSIASSGLESLISGSETNPADCLVDLIPNTQKGIVILRDIPFRSICE
HHLLPFVGTANLLYEPGKFLAGLGNLTSLVRCAARRLSIQERVTDQIADAIYTGLCARRVLVSLAARHTCILDEAGNTEV
TTFAHRGIGDADIISFMTIIR
>Mature_180_residues
TKLCRFESGSAQSAVANLLSALGQDVGRLKETPSIASSGLESLISGSETNPADCLVDLIPNTQKGIVILRDIPFRSICEH
HLLPFVGTANLLYEPGKFLAGLGNLTSLVRCAARRLSIQERVTDQIADAIYTGLCARRVLVSLAARHTCILDEAGNTEVT
TFAHRGIGDADIISFMTIIR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=177, Percent_Identity=35.5932203389831, Blast_Score=94, Evalue=6e-20,
Organism=Homo sapiens, GI4503949, Length=177, Percent_Identity=35.5932203389831, Blast_Score=94, Evalue=6e-20,
Organism=Homo sapiens, GI66932970, Length=125, Percent_Identity=40, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI66932972, Length=125, Percent_Identity=40, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788476, Length=127, Percent_Identity=36.2204724409449, Blast_Score=66, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560486, Length=142, Percent_Identity=32.3943661971831, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321706, Length=159, Percent_Identity=37.1069182389937, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24656782, Length=137, Percent_Identity=32.8467153284672, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24656796, Length=137, Percent_Identity=32.8467153284672, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656791, Length=137, Percent_Identity=32.8467153284672, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 19410; Mature: 19278

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLCRFESGSAQSAVANLLSALGQDVGRLKETPSIASSGLESLISGSETNPADCLVDLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHC
PNTQKGIVILRDIPFRSICEHHLLPFVGTANLLYEPGKFLAGLGNLTSLVRCAARRLSIQ
CCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERVTDQIADAIYTGLCARRVLVSLAARHTCILDEAGNTEVTTFAHRGIGDADIISFMTII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHHHHHHHHHH
R
C
>Mature Secondary Structure 
TKLCRFESGSAQSAVANLLSALGQDVGRLKETPSIASSGLESLISGSETNPADCLVDLI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHC
PNTQKGIVILRDIPFRSICEHHLLPFVGTANLLYEPGKFLAGLGNLTSLVRCAARRLSIQ
CCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERVTDQIADAIYTGLCARRVLVSLAARHTCILDEAGNTEVTTFAHRGIGDADIISFMTII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHHHHHHHHHH
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA