Click here to switch to the map view.

The map label for this gene is oppA [H]

Identifier: 28572309

GI number: 28572309

Start: 159968

End: 161569

Strand: Direct

Name: oppA [H]

Synonym: TW146

Alternate gene names: 28572309

Gene position: 159968-161569 (Clockwise)

Preceding gene: 28572308

Following gene: 28572312

Centisome position: 17.28

GC content: 44.82

Gene sequence:

>1602_bases
ATGAAATATATGAATTCTAGGTTCAAATTTTTTGCGGCTTTTATCCCGGTCGTGCTGTTCATGGCTGCGTGTTTCGGAGG
CTCTCCTGGCGTTAAGGGTAAAGAGATGCGCTTTGGAAGTTGTGAACCGCAGACCGCTCTTTTACCGGGATCTGTGCGTG
ATGAATGTGGCTCGGGCATTGTTACGAATCTTTTCGTTGGCCTTGTCACGCGCGATCGTGAGGGCAATCTGATAAATGAG
GTTGCAGAATCTATAGAAACTCAAGACAATCTGACCTACGTGATTAAGGTAAAAAAGGACTGGAAATTCTCTAATGGTGA
AAAAGTCACCGCGAAGAGTTTTGTTGACGCCTGGAATTTCACAGCGAAAAAATCCAATGCGCAGAGTAATCGTGACGACT
TGAAATATATTGATGGGTATAGTGCTGATCTGGATGGTGACCTTTCGGGTCTGAAAATTACCGACGAATTCACATTTACC
GTTAAGCTAAATAAGAAGCTGAATGACTTTTCTGAGCGGCTGAGCAATACTACTGCCTTTTTCCCGCTACCATCTGTTGC
ATATCAGGATATTAAAAAGTTCGGACAGAACCCAATAGGCAATGGGCCATACCTTCTGCACGATCGTCGTAAGAATGTTT
CGTGGTCTTATCGCCCTAATCGTGATTATCACGGTTATGCATCTCAATTCCCAATCCCGCCTTCTGTTCGTGTTACGGTC
TATCAGTCTGGATCCGCGAAATATGCTGACTATCTTGCCGGCAATCTTGATCTTGTTGATGTGCCGCAGGAGAATATCGA
AACTTACAAACAAGATTCTGAAGGTCGGCATATAGAGGGCGTTACATCTGTTGTATCGTATATCGGTATTACCGCCAACA
CCCCTGGCTTTGAACTGAATACTGAGGCTGGCAAACTGCGAAGAAGAGCATTGTCTTTGGCAATAGATCGGCAGGAAATG
GGTGATAAGCTCTATCATGGGCTAAGATTCCCAGCCACGGGTTTCTTTTCCTCCGCATTTTTCCCGAATTATGCCGACTT
ACCTGGCTCCGATGTATTGAAGTTCAATCTGGAGAAAGCCAAGGAGTTTTGGGCCGAAGCAGAAAAGCTATCTCCGTATC
CACGGCGCCAGATAGACTTCTTCTACAATGTGGATGGTGGCCATAAGATCTGGATCGATGCAGTTGTTAATCAGCTAAAG
AATGGACTTGGTATAACCAATATTATTCCGAAACCCGTTCCAACCTTTAGGGAATTTCTTGATCTGGTTGACCAAGATGA
TTTCAGTGGCTTCTTTAGGTCCGGCTGGCAAACAGGTTATATTGCTGCCCCCAGTATTGAGACTTTGTTTACTTCGGACG
GATCAAACAACTCTTCCCGTTACCGCAATCCGGAGTTTGATAAGCTTGCAGTGAGGGCGGTGGAAGCCAAAACATCTGAA
TCTGCTTCCCTGTATAAGGAGCTTCTTGGCATCCTTCTTAAGGATTTACCACTTATTCCGCTTTTTGGCTATAAAGATGC
CCTTGTCTATGGTAAGGACGTCAAGGGTATAAAACTGAATAAGCGAGGAATTGTTGACACCTCTGACATGATCAGGGAAT
AG

Upstream 100 bases:

>100_bases
CGCTTTTTTATGACCGCGGTGGTCTTGTATGGTCAAAGAATGTCAGCAATGTTGAAAGCAACTGGGTTGGTGTTCCGGTT
TACTATCTGATAACGAAGGG

Downstream 100 bases:

>100_bases
TCAATCCTCAAATCCGCGTGAAGCGATTGCTGTTGCTATTTCCTGAGCCATATTAAGGCTTAGGACTGTAAATGGCAGGA
AGATTGCGAGGGGCTTTTTT

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKYMNSRFKFFAAFIPVVLFMAACFGGSPGVKGKEMRFGSCEPQTALLPGSVRDECGSGIVTNLFVGLVTRDREGNLINE
VAESIETQDNLTYVIKVKKDWKFSNGEKVTAKSFVDAWNFTAKKSNAQSNRDDLKYIDGYSADLDGDLSGLKITDEFTFT
VKLNKKLNDFSERLSNTTAFFPLPSVAYQDIKKFGQNPIGNGPYLLHDRRKNVSWSYRPNRDYHGYASQFPIPPSVRVTV
YQSGSAKYADYLAGNLDLVDVPQENIETYKQDSEGRHIEGVTSVVSYIGITANTPGFELNTEAGKLRRRALSLAIDRQEM
GDKLYHGLRFPATGFFSSAFFPNYADLPGSDVLKFNLEKAKEFWAEAEKLSPYPRRQIDFFYNVDGGHKIWIDAVVNQLK
NGLGITNIIPKPVPTFREFLDLVDQDDFSGFFRSGWQTGYIAAPSIETLFTSDGSNNSSRYRNPEFDKLAVRAVEAKTSE
SASLYKELLGILLKDLPLIPLFGYKDALVYGKDVKGIKLNKRGIVDTSDMIRE

Sequences:

>Translated_533_residues
MKYMNSRFKFFAAFIPVVLFMAACFGGSPGVKGKEMRFGSCEPQTALLPGSVRDECGSGIVTNLFVGLVTRDREGNLINE
VAESIETQDNLTYVIKVKKDWKFSNGEKVTAKSFVDAWNFTAKKSNAQSNRDDLKYIDGYSADLDGDLSGLKITDEFTFT
VKLNKKLNDFSERLSNTTAFFPLPSVAYQDIKKFGQNPIGNGPYLLHDRRKNVSWSYRPNRDYHGYASQFPIPPSVRVTV
YQSGSAKYADYLAGNLDLVDVPQENIETYKQDSEGRHIEGVTSVVSYIGITANTPGFELNTEAGKLRRRALSLAIDRQEM
GDKLYHGLRFPATGFFSSAFFPNYADLPGSDVLKFNLEKAKEFWAEAEKLSPYPRRQIDFFYNVDGGHKIWIDAVVNQLK
NGLGITNIIPKPVPTFREFLDLVDQDDFSGFFRSGWQTGYIAAPSIETLFTSDGSNNSSRYRNPEFDKLAVRAVEAKTSE
SASLYKELLGILLKDLPLIPLFGYKDALVYGKDVKGIKLNKRGIVDTSDMIRE
>Mature_533_residues
MKYMNSRFKFFAAFIPVVLFMAACFGGSPGVKGKEMRFGSCEPQTALLPGSVRDECGSGIVTNLFVGLVTRDREGNLINE
VAESIETQDNLTYVIKVKKDWKFSNGEKVTAKSFVDAWNFTAKKSNAQSNRDDLKYIDGYSADLDGDLSGLKITDEFTFT
VKLNKKLNDFSERLSNTTAFFPLPSVAYQDIKKFGQNPIGNGPYLLHDRRKNVSWSYRPNRDYHGYASQFPIPPSVRVTV
YQSGSAKYADYLAGNLDLVDVPQENIETYKQDSEGRHIEGVTSVVSYIGITANTPGFELNTEAGKLRRRALSLAIDRQEM
GDKLYHGLRFPATGFFSSAFFPNYADLPGSDVLKFNLEKAKEFWAEAEKLSPYPRRQIDFFYNVDGGHKIWIDAVVNQLK
NGLGITNIIPKPVPTFREFLDLVDQDDFSGFFRSGWQTGYIAAPSIETLFTSDGSNNSSRYRNPEFDKLAVRAVEAKTSE
SASLYKELLGILLKDLPLIPLFGYKDALVYGKDVKGIKLNKRGIVDTSDMIRE

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI87081878, Length=479, Percent_Identity=25.2609603340292, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789397, Length=511, Percent_Identity=26.027397260274, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787495, Length=487, Percent_Identity=25.8726899383984, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787052, Length=530, Percent_Identity=20.7547169811321, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59784; Mature: 59784

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYMNSRFKFFAAFIPVVLFMAACFGGSPGVKGKEMRFGSCEPQTALLPGSVRDECGSGI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHCCCH
VTNLFVGLVTRDREGNLINEVAESIETQDNLTYVIKVKKDWKFSNGEKVTAKSFVDAWNF
HHHHHHHHEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHCC
TAKKSNAQSNRDDLKYIDGYSADLDGDLSGLKITDEFTFTVKLNKKLNDFSERLSNTTAF
CCCCCCCCCCCCCHHEECCCCCCCCCCCCCEEEECEEEEEEEECCHHHHHHHHHCCCEEE
FPLPSVAYQDIKKFGQNPIGNGPYLLHDRRKNVSWSYRPNRDYHGYASQFPIPPSVRVTV
CCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEE
YQSGSAKYADYLAGNLDLVDVPQENIETYKQDSEGRHIEGVTSVVSYIGITANTPGFELN
EECCCCCHHHHHCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEC
TEAGKLRRRALSLAIDRQEMGDKLYHGLRFPATGFFSSAFFPNYADLPGSDVLKFNLEKA
CHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEHHHH
KEFWAEAEKLSPYPRRQIDFFYNVDGGHKIWIDAVVNQLKNGLGITNIIPKPVPTFREFL
HHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
DLVDQDDFSGFFRSGWQTGYIAAPSIETLFTSDGSNNSSRYRNPEFDKLAVRAVEAKTSE
HHHCCCCCCHHHHCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCH
SASLYKELLGILLKDLPLIPLFGYKDALVYGKDVKGIKLNKRGIVDTSDMIRE
HHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCCEEECCCCCCCCHHHHCC
>Mature Secondary Structure
MKYMNSRFKFFAAFIPVVLFMAACFGGSPGVKGKEMRFGSCEPQTALLPGSVRDECGSGI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHCCCH
VTNLFVGLVTRDREGNLINEVAESIETQDNLTYVIKVKKDWKFSNGEKVTAKSFVDAWNF
HHHHHHHHEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHCC
TAKKSNAQSNRDDLKYIDGYSADLDGDLSGLKITDEFTFTVKLNKKLNDFSERLSNTTAF
CCCCCCCCCCCCCHHEECCCCCCCCCCCCCEEEECEEEEEEEECCHHHHHHHHHCCCEEE
FPLPSVAYQDIKKFGQNPIGNGPYLLHDRRKNVSWSYRPNRDYHGYASQFPIPPSVRVTV
CCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEE
YQSGSAKYADYLAGNLDLVDVPQENIETYKQDSEGRHIEGVTSVVSYIGITANTPGFELN
EECCCCCHHHHHCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEC
TEAGKLRRRALSLAIDRQEMGDKLYHGLRFPATGFFSSAFFPNYADLPGSDVLKFNLEKA
CHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEHHHH
KEFWAEAEKLSPYPRRQIDFFYNVDGGHKIWIDAVVNQLKNGLGITNIIPKPVPTFREFL
HHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
DLVDQDDFSGFFRSGWQTGYIAAPSIETLFTSDGSNNSSRYRNPEFDKLAVRAVEAKTSE
HHHCCCCCCHHHHCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCH
SASLYKELLGILLKDLPLIPLFGYKDALVYGKDVKGIKLNKRGIVDTSDMIRE
HHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCCEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]