Click here to switch to the map view.

The map label for this gene is oppA [H]

Identifier: 28572308

GI number: 28572308

Start: 158391

End: 159971

Strand: Direct

Name: oppA [H]

Synonym: TW145

Alternate gene names: 28572308

Gene position: 158391-159971 (Clockwise)

Preceding gene: 28572307

Following gene: 28572309

Centisome position: 17.11

GC content: 47.69

Gene sequence:

>1581_bases
ATGCGTAAGGGATTTTCGAGGGTCTTTACTTTTACGGCAGTCTCAGCCGTGCTGTTTGTGACAGCATGTTTTGGTGGTAA
GCCTACCGATTCGTATGTCAATGTGAATACGACTAAGATCAGCACAGGCCTGGTTCCCGCTAATTCCAATGAGCTGTCGG
CTCACAGGATTCTCAGCATGCTTTTTTCCGGCCTTGTATATATTGACAAAGATGCGCGCATCCAAAACGAGGTTGCAAAA
TCCATAGAGACCGCAGATAATCGCGTGTGGACCATTACCCTGAGGGAGGATTTTAAATTCTCTAATGGTGAAAAAGTCAC
CGCGAAGAGTTTTGTTGACGCCTGGAATTTCGCGGCCAAGATTTCCAATGCAATGGGTAACCGTGATTTTCTGGGTAACA
TAGAGGGTTTCACAGAAAAGGGTGATACCGACCTGTCTGGGTTAAAAATTCTGGATGAATACAAGTTTCGCGTTATTCTA
AAGAGCCCTAACCGGAATTTTGTTGCCAAGTTGTCGTATTCAGTTTTTTGGCCGCTTCCGTCGGATGCGTATAAGGATAT
TCGGGCTTTTGGCTTGAAGCCCATCGGTAATGGCCCATACTCTCTTGTCAGTTTCACGCAGGATGTTGAGGCTGTGCTGA
AAAAAAATCCTGATTACAAGGGGCCAAGGCAGCCTCGTAATTCTGGCCTCAGGTACAAGATATATTCCGACCCCGGGCCT
GCGTATGCTGACGTTTTGTCTGATAGCTCTGATGTCACAGATATAATCCCACCGAATGCCCAGGAAAAGTTCCAGTCCGA
TTTTCCTTCTCGTTGGGTTAGGCGTGAGATCGCGGCAACTATATACATCGCCATTCCTGGATACGTTGCGCATTTTGCTT
TTGACAGGGAGGGAATCCTGCGCAGGAAGGCTGTTTCTATGGCAATTAACCGGCAGGATATAGCTGATAAGATTTACCAC
GGCGCCAGAGTGCCTGCGCGAGACTTTACTTCTCCGTCAGTGCTGGGGTACAAGGCCGGCCTGCCGGGCTCGGATGTTCT
GAAATATGATCCTGAGCGAGCCAAGCAGTTATGGGCACAGGCTGATGCTATATCCCCTTGGGGCTCGGGTGTTTTGTATC
TTAACTTTGCCGCTACCCGCGGCGATAAAGCCCTATTCGAAGCTTTGGCGAATTCCATAAAGAACGTTCTTGGTATAGAG
GTGCAGGCCTATCCCAATACTGATTGGAAGGCAAATCTTCTGCATGGCCAGCAAACGAAGCAGCCTTTTAGAATTGGCTG
GGCCGCTGACTGGCCGGCTATAGACACTTACCTTGGGTCGCTGTTTCATAGTCAAGCTTCTAACAATTATTACGAGTTTC
GTAATGCGGAGTATGACTCACTGCTGGTCAAGGCCGCAGAGGCTTTGACCTTTCCGAAAGCTCAAGATTACTATGACAAA
CTGCAGGAAATTTTATTCCAGAATATGCCTGTTGTACCGCTTTTTTATGACCGCGGTGGTCTTGTATGGTCAAAGAATGT
CAGCAATGTTGAAAGCAACTGGGTTGGTGTTCCGGTTTACTATCTGATAACGAAGGGATGA

Upstream 100 bases:

>100_bases
TGTAAATCGCCTAAGCTAGTGGGACATCTGCAAAGCAAAATTTGTCTAAATGAAAAGTTTTGTGTAGGATCATGCACAAG
ATATTCGTTGGGGTAGGGGT

Downstream 100 bases:

>100_bases
AATATATGAATTCTAGGTTCAAATTTTTTGCGGCTTTTATCCCGGTCGTGCTGTTCATGGCTGCGTGTTTCGGAGGCTCT
CCTGGCGTTAAGGGTAAAGA

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MRKGFSRVFTFTAVSAVLFVTACFGGKPTDSYVNVNTTKISTGLVPANSNELSAHRILSMLFSGLVYIDKDARIQNEVAK
SIETADNRVWTITLREDFKFSNGEKVTAKSFVDAWNFAAKISNAMGNRDFLGNIEGFTEKGDTDLSGLKILDEYKFRVIL
KSPNRNFVAKLSYSVFWPLPSDAYKDIRAFGLKPIGNGPYSLVSFTQDVEAVLKKNPDYKGPRQPRNSGLRYKIYSDPGP
AYADVLSDSSDVTDIIPPNAQEKFQSDFPSRWVRREIAATIYIAIPGYVAHFAFDREGILRRKAVSMAINRQDIADKIYH
GARVPARDFTSPSVLGYKAGLPGSDVLKYDPERAKQLWAQADAISPWGSGVLYLNFAATRGDKALFEALANSIKNVLGIE
VQAYPNTDWKANLLHGQQTKQPFRIGWAADWPAIDTYLGSLFHSQASNNYYEFRNAEYDSLLVKAAEALTFPKAQDYYDK
LQEILFQNMPVVPLFYDRGGLVWSKNVSNVESNWVGVPVYYLITKG

Sequences:

>Translated_526_residues
MRKGFSRVFTFTAVSAVLFVTACFGGKPTDSYVNVNTTKISTGLVPANSNELSAHRILSMLFSGLVYIDKDARIQNEVAK
SIETADNRVWTITLREDFKFSNGEKVTAKSFVDAWNFAAKISNAMGNRDFLGNIEGFTEKGDTDLSGLKILDEYKFRVIL
KSPNRNFVAKLSYSVFWPLPSDAYKDIRAFGLKPIGNGPYSLVSFTQDVEAVLKKNPDYKGPRQPRNSGLRYKIYSDPGP
AYADVLSDSSDVTDIIPPNAQEKFQSDFPSRWVRREIAATIYIAIPGYVAHFAFDREGILRRKAVSMAINRQDIADKIYH
GARVPARDFTSPSVLGYKAGLPGSDVLKYDPERAKQLWAQADAISPWGSGVLYLNFAATRGDKALFEALANSIKNVLGIE
VQAYPNTDWKANLLHGQQTKQPFRIGWAADWPAIDTYLGSLFHSQASNNYYEFRNAEYDSLLVKAAEALTFPKAQDYYDK
LQEILFQNMPVVPLFYDRGGLVWSKNVSNVESNWVGVPVYYLITKG
>Mature_526_residues
MRKGFSRVFTFTAVSAVLFVTACFGGKPTDSYVNVNTTKISTGLVPANSNELSAHRILSMLFSGLVYIDKDARIQNEVAK
SIETADNRVWTITLREDFKFSNGEKVTAKSFVDAWNFAAKISNAMGNRDFLGNIEGFTEKGDTDLSGLKILDEYKFRVIL
KSPNRNFVAKLSYSVFWPLPSDAYKDIRAFGLKPIGNGPYSLVSFTQDVEAVLKKNPDYKGPRQPRNSGLRYKIYSDPGP
AYADVLSDSSDVTDIIPPNAQEKFQSDFPSRWVRREIAATIYIAIPGYVAHFAFDREGILRRKAVSMAINRQDIADKIYH
GARVPARDFTSPSVLGYKAGLPGSDVLKYDPERAKQLWAQADAISPWGSGVLYLNFAATRGDKALFEALANSIKNVLGIE
VQAYPNTDWKANLLHGQQTKQPFRIGWAADWPAIDTYLGSLFHSQASNNYYEFRNAEYDSLLVKAAEALTFPKAQDYYDK
LQEILFQNMPVVPLFYDRGGLVWSKNVSNVESNWVGVPVYYLITKG

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=469, Percent_Identity=26.4392324093817, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081878, Length=486, Percent_Identity=25.5144032921811, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789397, Length=482, Percent_Identity=24.4813278008299, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787052, Length=507, Percent_Identity=22.4852071005917, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789966, Length=386, Percent_Identity=23.8341968911917, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58850; Mature: 58850

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGFSRVFTFTAVSAVLFVTACFGGKPTDSYVNVNTTKISTGLVPANSNELSAHRILSM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHH
LFSGLVYIDKDARIQNEVAKSIETADNRVWTITLREDFKFSNGEKVTAKSFVDAWNFAAK
HHCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHH
ISNAMGNRDFLGNIEGFTEKGDTDLSGLKILDEYKFRVILKSPNRNFVAKLSYSVFWPLP
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHEEECCEEEEEEECCCCCEEEEEEEEEEECCC
SDAYKDIRAFGLKPIGNGPYSLVSFTQDVEAVLKKNPDYKGPRQPRNSGLRYKIYSDPGP
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
AYADVLSDSSDVTDIIPPNAQEKFQSDFPSRWVRREIAATIYIAIPGYVAHFAFDREGIL
HHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHEEEEEEECCHHHHHHCCCHHHH
RRKAVSMAINRQDIADKIYHGARVPARDFTSPSVLGYKAGLPGSDVLKYDPERAKQLWAQ
HHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
ADAISPWGSGVLYLNFAATRGDKALFEALANSIKNVLGIEVQAYPNTDWKANLLHGQQTK
HCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCEEEECCCCCCC
QPFRIGWAADWPAIDTYLGSLFHSQASNNYYEFRNAEYDSLLVKAAEALTFPKAQDYYDK
CCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHH
LQEILFQNMPVVPLFYDRGGLVWSKNVSNVESNWVGVPVYYLITKG
HHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRKGFSRVFTFTAVSAVLFVTACFGGKPTDSYVNVNTTKISTGLVPANSNELSAHRILSM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHH
LFSGLVYIDKDARIQNEVAKSIETADNRVWTITLREDFKFSNGEKVTAKSFVDAWNFAAK
HHCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHH
ISNAMGNRDFLGNIEGFTEKGDTDLSGLKILDEYKFRVILKSPNRNFVAKLSYSVFWPLP
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHEEECCEEEEEEECCCCCEEEEEEEEEEECCC
SDAYKDIRAFGLKPIGNGPYSLVSFTQDVEAVLKKNPDYKGPRQPRNSGLRYKIYSDPGP
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
AYADVLSDSSDVTDIIPPNAQEKFQSDFPSRWVRREIAATIYIAIPGYVAHFAFDREGIL
HHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHEEEEEEECCHHHHHHCCCHHHH
RRKAVSMAINRQDIADKIYHGARVPARDFTSPSVLGYKAGLPGSDVLKYDPERAKQLWAQ
HHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
ADAISPWGSGVLYLNFAATRGDKALFEALANSIKNVLGIEVQAYPNTDWKANLLHGQQTK
HCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCEEEECCCCCCC
QPFRIGWAADWPAIDTYLGSLFHSQASNNYYEFRNAEYDSLLVKAAEALTFPKAQDYYDK
CCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHH
LQEILFQNMPVVPLFYDRGGLVWSKNVSNVESNWVGVPVYYLITKG
HHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]