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The map label for this gene is cls [H]

Identifier: 28572244

GI number: 28572244

Start: 65832

End: 67328

Strand: Direct

Name: cls [H]

Synonym: TW074

Alternate gene names: 28572244

Gene position: 65832-67328 (Clockwise)

Preceding gene: 28572243

Following gene: 28572245

Centisome position: 7.11

GC content: 43.62

Gene sequence:

>1497_bases
GTGAAGCCCTATTTGTTTATTTTGGCGGCCCTGCTATTGGCCGACCTTGTCATCCGTATTGGCTGCCTTTTTGTTGTTCC
TCGCAATAGACGTCCTAATTCTGCCAACGCCTGGCTTCTCACAATATTCTTTCTGCCGTTTATTGGATGGTTTCTTTTTT
TGATAATAGGCAGCTACAAGCTCCCAAAATCAAGAAGAGAAAAACAGGCTCTTATAAACCAGCTTGTTGCAAATGCACTG
TCAGATCATGCAGTATTGGACAATGTAGATCTGCAATGGCTACCATCTCTGGTAAAACTAAACAGAAATCTTGGATCTAT
GCCGATGGTTGCGGGAACTCGTGTACATATTCTTACTGATACAAGTGTGATTATGGAGCAGATGGTATCTGCCATTGATT
CAGCTAAAAGCAGTGTACACGTTGAGTTCTACATCTTTTCGCTGGATGAATTTACGCAGGGCTTTTTCACAAGCCTTGAA
AATGCGACCGCCCGCGGGGTAAAGGTTCGGGTTTTATATGATCACATCGCAACTTTGCGCGTCCCTGGATATCTGAATAT
GAAGTCTCGCCTTAAGCGCAATGGGATTGAATTTTATCCGATGCTTCCGATTCAGCCCTTCAAGTTGAAATATCAGAGGC
CTGACCTGCGCAATCATCGAAAATTGCTTGTAATTGATGATGCAATTGCATTTGTTGGATCGCTAAACATTATTGATCCT
TTTTATAACACGAGGGGTCGCAAGAAAATGTTTTGGCAAGATGCGATGCTGCGCCTTGAAGGACCCGTTATAAGCGGGAT
AAGCGCGGTTTTTGTCTCTGATTGGTACGCAGAAACAGGCCAGCTCCTGAGAGACTACAGAATTGTAAAAACTTCATCCG
GCGATTCCTTCACAGAAAACCGCACACAATCAAACAGTGTTGATAGGAGTTGCCCGAGTACAAATAACGTTTTATGTCAA
GTAGTTCCTTCTGGTCCTGGATTTGAGGGGGAAAATAACCTCCAATTGTTTCTAATGCTCCTTTATGCATCGCAAAAACG
GGTTGCAATTACATCACCGTACTTCGTGCCGGACGAGGCAATGACTTTAGCAATCGTGTCGGCGACTAAAAGGGGGGTTA
GTGTCGAACTGTTTTTATCTGAAAACCATGACCAAAAAATTGTTTACCACGCTCAGTGTTCGTACTACGAACAGCTATTG
AGAAGCGGCGTAAAGATTTGGCTATACCGTGCACCAAATTTCTTACACAGCAAATATGTAACTGTTGATGATGATGTTGC
CGTTTTTGGATCAAGCAATTTTGATATGCGCTCTTTCAATCTGAATATGGAAATGTCACTGTTGCTCTACAGCAAGGAAT
TTGTTGCAAAACTGCAGGCGGTTGAACAGGAAAACCGAGCAAATAGCTCCCTGCTCACCCTCGATGAATGGATGCACCGC
GGGAAAATGGACCGCTTGAAAGATAATCTAGCTAGACTTACATCCGCCCTGCAATAG

Upstream 100 bases:

>100_bases
CCACACCTGAGACATTAAGCCCTGAGACATTAGGCACCGACTCAGCCGATAAGCGATCTCGCGGAGATTCACAACAGGGC
ACCTTGCCACCCGGTGGGTC

Downstream 100 bases:

>100_bases
GCACTTATTGAAGAAAAATTTAGGATTCACTCTCGCTGTTTTCTAGTATCCTTGGGTGAGGATATGTTTGAAAGGTTTAC
AGATAAAGCCCGCCGGGTTA

Product: phospholipase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MKPYLFILAALLLADLVIRIGCLFVVPRNRRPNSANAWLLTIFFLPFIGWFLFLIIGSYKLPKSRREKQALINQLVANAL
SDHAVLDNVDLQWLPSLVKLNRNLGSMPMVAGTRVHILTDTSVIMEQMVSAIDSAKSSVHVEFYIFSLDEFTQGFFTSLE
NATARGVKVRVLYDHIATLRVPGYLNMKSRLKRNGIEFYPMLPIQPFKLKYQRPDLRNHRKLLVIDDAIAFVGSLNIIDP
FYNTRGRKKMFWQDAMLRLEGPVISGISAVFVSDWYAETGQLLRDYRIVKTSSGDSFTENRTQSNSVDRSCPSTNNVLCQ
VVPSGPGFEGENNLQLFLMLLYASQKRVAITSPYFVPDEAMTLAIVSATKRGVSVELFLSENHDQKIVYHAQCSYYEQLL
RSGVKIWLYRAPNFLHSKYVTVDDDVAVFGSSNFDMRSFNLNMEMSLLLYSKEFVAKLQAVEQENRANSSLLTLDEWMHR
GKMDRLKDNLARLTSALQ

Sequences:

>Translated_498_residues
MKPYLFILAALLLADLVIRIGCLFVVPRNRRPNSANAWLLTIFFLPFIGWFLFLIIGSYKLPKSRREKQALINQLVANAL
SDHAVLDNVDLQWLPSLVKLNRNLGSMPMVAGTRVHILTDTSVIMEQMVSAIDSAKSSVHVEFYIFSLDEFTQGFFTSLE
NATARGVKVRVLYDHIATLRVPGYLNMKSRLKRNGIEFYPMLPIQPFKLKYQRPDLRNHRKLLVIDDAIAFVGSLNIIDP
FYNTRGRKKMFWQDAMLRLEGPVISGISAVFVSDWYAETGQLLRDYRIVKTSSGDSFTENRTQSNSVDRSCPSTNNVLCQ
VVPSGPGFEGENNLQLFLMLLYASQKRVAITSPYFVPDEAMTLAIVSATKRGVSVELFLSENHDQKIVYHAQCSYYEQLL
RSGVKIWLYRAPNFLHSKYVTVDDDVAVFGSSNFDMRSFNLNMEMSLLLYSKEFVAKLQAVEQENRANSSLLTLDEWMHR
GKMDRLKDNLARLTSALQ
>Mature_498_residues
MKPYLFILAALLLADLVIRIGCLFVVPRNRRPNSANAWLLTIFFLPFIGWFLFLIIGSYKLPKSRREKQALINQLVANAL
SDHAVLDNVDLQWLPSLVKLNRNLGSMPMVAGTRVHILTDTSVIMEQMVSAIDSAKSSVHVEFYIFSLDEFTQGFFTSLE
NATARGVKVRVLYDHIATLRVPGYLNMKSRLKRNGIEFYPMLPIQPFKLKYQRPDLRNHRKLLVIDDAIAFVGSLNIIDP
FYNTRGRKKMFWQDAMLRLEGPVISGISAVFVSDWYAETGQLLRDYRIVKTSSGDSFTENRTQSNSVDRSCPSTNNVLCQ
VVPSGPGFEGENNLQLFLMLLYASQKRVAITSPYFVPDEAMTLAIVSATKRGVSVELFLSENHDQKIVYHAQCSYYEQLL
RSGVKIWLYRAPNFLHSKYVTVDDDVAVFGSSNFDMRSFNLNMEMSLLLYSKEFVAKLQAVEQENRANSSLLTLDEWMHR
GKMDRLKDNLARLTSALQ

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=488, Percent_Identity=30.1229508196721, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1787007, Length=350, Percent_Identity=26.8571428571429, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87081826, Length=410, Percent_Identity=24.390243902439, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: NA

EC number: 2.7.8.-

Molecular weight: Translated: 56777; Mature: 56777

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYLFILAALLLADLVIRIGCLFVVPRNRRPNSANAWLLTIFFLPFIGWFLFLIIGSYK
CCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LPKSRREKQALINQLVANALSDHAVLDNVDLQWLPSLVKLNRNLGSMPMVAGTRVHILTD
CCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
TSVIMEQMVSAIDSAKSSVHVEFYIFSLDEFTQGFFTSLENATARGVKVRVLYDHIATLR
HHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHEE
VPGYLNMKSRLKRNGIEFYPMLPIQPFKLKYQRPDLRNHRKLLVIDDAIAFVGSLNIIDP
CCCCCCHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCEEEEEEHHHHHHCCCCEECC
FYNTRGRKKMFWQDAMLRLEGPVISGISAVFVSDWYAETGQLLRDYRIVKTSSGDSFTEN
CCCCCCCCHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
RTQSNSVDRSCPSTNNVLCQVVPSGPGFEGENNLQLFLMLLYASQKRVAITSPYFVPDEA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHEEHHCCCCCEEEECCEECCCCH
MTLAIVSATKRGVSVELFLSENHDQKIVYHAQCSYYEQLLRSGVKIWLYRAPNFLHSKYV
HEEEEEECCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHCCCEEEEEECCCCHHCCEE
TVDDDVAVFGSSNFDMRSFNLNMEMSLLLYSKEFVAKLQAVEQENRANSSLLTLDEWMHR
EECCCEEEECCCCCCEEEEECCEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHC
GKMDRLKDNLARLTSALQ
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPYLFILAALLLADLVIRIGCLFVVPRNRRPNSANAWLLTIFFLPFIGWFLFLIIGSYK
CCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LPKSRREKQALINQLVANALSDHAVLDNVDLQWLPSLVKLNRNLGSMPMVAGTRVHILTD
CCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
TSVIMEQMVSAIDSAKSSVHVEFYIFSLDEFTQGFFTSLENATARGVKVRVLYDHIATLR
HHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHEE
VPGYLNMKSRLKRNGIEFYPMLPIQPFKLKYQRPDLRNHRKLLVIDDAIAFVGSLNIIDP
CCCCCCHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCEEEEEEHHHHHHCCCCEECC
FYNTRGRKKMFWQDAMLRLEGPVISGISAVFVSDWYAETGQLLRDYRIVKTSSGDSFTEN
CCCCCCCCHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
RTQSNSVDRSCPSTNNVLCQVVPSGPGFEGENNLQLFLMLLYASQKRVAITSPYFVPDEA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHEEHHCCCCCEEEECCEECCCCH
MTLAIVSATKRGVSVELFLSENHDQKIVYHAQCSYYEQLLRSGVKIWLYRAPNFLHSKYV
HEEEEEECCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHCCCEEEEEECCCCHHCCEE
TVDDDVAVFGSSNFDMRSFNLNMEMSLLLYSKEFVAKLQAVEQENRANSSLLTLDEWMHR
EECCCEEEECCCCCCEEEEECCEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHC
GKMDRLKDNLARLTSALQ
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12219091 [H]