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The map label for this gene is rmlA [H]

Identifier: 28572209

GI number: 28572209

Start: 32571

End: 33449

Strand: Direct

Name: rmlA [H]

Synonym: TW036

Alternate gene names: 28572209

Gene position: 32571-33449 (Clockwise)

Preceding gene: 28572208

Following gene: 28572213

Centisome position: 3.52

GC content: 45.05

Gene sequence:

>879_bases
TTGAAGGGTATTATCCTAGCTGGTGGAACCGGATCAAGGCTCTGGCCCATAACAAAATGCATCTCGAAACAATTGATGCC
TGTTTACGATAAGCCGATGATCTATTATCCCCTTTCCACTCTTATGATGGCCGATATTCGGGAAATCCTGGTTATAACAA
CCACGCAGGATTATGACCAATTTCGCAGACTTTTAGGTGATGGCGGGCAGTGGGGGATCAAGTTGTCGTACGCACAACAG
TTGTCACCCGGTGGATTGGCCCAGGCATTTCTTATTGGCGAGTCTTTTATCGCCAGCGAGAGTATTGCGCTTGTACTTGG
TGACAATATATTCCACGGAAGCGGTCTTGGTACATCGCTTCGTCAGCATCGAAGCATTCAGGGTGCCCTCATTTTTGCTT
ATCACGTCAGTAATCCAAAGGATTACGGAGTTGTTACCTTTGATGCAGATGGTAAAGCGGTATCAATTGAAGAAAAGCCA
GAAAGTCCAAAAAGTAATTTTGCAGTTCCGGGACTTTATTTCTATGACAACCAGGTAGTTGATATAGCAAAACAGATTCC
TTTGAGCGCTCGCGGCGAGCTGGAAATAAGTTCAGTGAATAACTTTTATTTAGAGAAAGATCAGCTTAGAGTTTATCCAT
TGGCCCGTGGAACCTCTTGGCTCGATACCGGCACATTTGATTCTCTTGTACAGGCAAGCGAGTATGTCCGCGTTATAGAG
CAAAGACAGGGATTTAAAGTTGGTTGTGTCGAGGAAATAGCCTGGAGGGCTGGATGGATAGATGATGATCAGCTGGCCAA
ACTTGCAAATGGGCTTATGAAAAGTGGTTATGGGAAGTATTTGCACTACCTCCTGAGCGGTAAGCACGGGTTTTCATAA

Upstream 100 bases:

>100_bases
GATGAATGCCGGGCAACACCCGAGACACACGGAAACATTCGATAAATACCTTGGGCCATTCTAGCCAAGGCCGGGCCAAG
TATGTTTTTAAGGAGATAGC

Downstream 100 bases:

>100_bases
AAACTCACTACTTCAGTTTTCTTGCCTGTAGACGGTACCTGAGTTTCAGTCCAAGTTTCAGAAGGAATGTAACGGGTTTC
TGCCATTTTTTTGAGTATTT

Product: nucleotidyl transferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKGIILAGGTGSRLWPITKCISKQLMPVYDKPMIYYPLSTLMMADIREILVITTTQDYDQFRRLLGDGGQWGIKLSYAQQ
LSPGGLAQAFLIGESFIASESIALVLGDNIFHGSGLGTSLRQHRSIQGALIFAYHVSNPKDYGVVTFDADGKAVSIEEKP
ESPKSNFAVPGLYFYDNQVVDIAKQIPLSARGELEISSVNNFYLEKDQLRVYPLARGTSWLDTGTFDSLVQASEYVRVIE
QRQGFKVGCVEEIAWRAGWIDDDQLAKLANGLMKSGYGKYLHYLLSGKHGFS

Sequences:

>Translated_292_residues
MKGIILAGGTGSRLWPITKCISKQLMPVYDKPMIYYPLSTLMMADIREILVITTTQDYDQFRRLLGDGGQWGIKLSYAQQ
LSPGGLAQAFLIGESFIASESIALVLGDNIFHGSGLGTSLRQHRSIQGALIFAYHVSNPKDYGVVTFDADGKAVSIEEKP
ESPKSNFAVPGLYFYDNQVVDIAKQIPLSARGELEISSVNNFYLEKDQLRVYPLARGTSWLDTGTFDSLVQASEYVRVIE
QRQGFKVGCVEEIAWRAGWIDDDQLAKLANGLMKSGYGKYLHYLLSGKHGFS
>Mature_292_residues
MKGIILAGGTGSRLWPITKCISKQLMPVYDKPMIYYPLSTLMMADIREILVITTTQDYDQFRRLLGDGGQWGIKLSYAQQ
LSPGGLAQAFLIGESFIASESIALVLGDNIFHGSGLGTSLRQHRSIQGALIFAYHVSNPKDYGVVTFDADGKAVSIEEKP
ESPKSNFAVPGLYFYDNQVVDIAKQIPLSARGELEISSVNNFYLEKDQLRVYPLARGTSWLDTGTFDSLVQASEYVRVIE
QRQGFKVGCVEEIAWRAGWIDDDQLAKLANGLMKSGYGKYLHYLLSGKHGFS

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide,

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=289, Percent_Identity=59.1695501730104, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788351, Length=284, Percent_Identity=58.8028169014084, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI133931050, Length=207, Percent_Identity=25.1207729468599, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=262, Percent_Identity=24.4274809160305, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21355443, Length=249, Percent_Identity=24.4979919678715, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644084, Length=249, Percent_Identity=24.4979919678715, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32337; Mature: 32337

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGTGSRLWPITKCISKQLMPVYDKPMIYYPLSTLMMADIREILVITTTQDYDQ
CCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHEEEEEEECCCHHH
FRRLLGDGGQWGIKLSYAQQLSPGGLAQAFLIGESFIASESIALVLGDNIFHGSGLGTSL
HHHHHCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCHHH
RQHRSIQGALIFAYHVSNPKDYGVVTFDADGKAVSIEEKPESPKSNFAVPGLYFYDNQVV
HHHCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCHHH
DIAKQIPLSARGELEISSVNNFYLEKDQLRVYPLARGTSWLDTGTFDSLVQASEYVRVIE
HHHHHCCCCCCCCEEEEECCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
QRQGFKVGCVEEIAWRAGWIDDDQLAKLANGLMKSGYGKYLHYLLSGKHGFS
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGIILAGGTGSRLWPITKCISKQLMPVYDKPMIYYPLSTLMMADIREILVITTTQDYDQ
CCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHEEEEEEECCCHHH
FRRLLGDGGQWGIKLSYAQQLSPGGLAQAFLIGESFIASESIALVLGDNIFHGSGLGTSL
HHHHHCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCHHH
RQHRSIQGALIFAYHVSNPKDYGVVTFDADGKAVSIEEKPESPKSNFAVPGLYFYDNQVV
HHHCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCHHH
DIAKQIPLSARGELEISSVNNFYLEKDQLRVYPLARGTSWLDTGTFDSLVQASEYVRVIE
HHHHHCCCCCCCCEEEEECCCEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
QRQGFKVGCVEEIAWRAGWIDDDQLAKLANGLMKSGYGKYLHYLLSGKHGFS
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084178 [H]