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The map label for this gene is recF

Identifier: 28572178

GI number: 28572178

Start: 2890

End: 3981

Strand: Direct

Name: recF

Synonym: TW003

Alternate gene names: 28572178

Gene position: 2890-3981 (Clockwise)

Preceding gene: 28572177

Following gene: 28572179

Centisome position: 0.31

GC content: 38.92

Gene sequence:

>1092_bases
TTGCAAAATAATTTGATTTCTCATATAAACCTAAGGAATTTTCGAAATTATGAGTACCAAAGTATTTCATTTACTGATGG
TCTCAACCTTATAAGGGGTGATAATGGCCAGGGAAAGACCAACCTTGCTGAGGCAATCTATTTTCTGTCGGGTTTCGGAT
CTCACAGGACATACAAAAACCAGCCTCTTATAAAATCGGGAGAACAGAAAGCAGAGATATCTGCAAAAATTCAGTCTAAA
TACGGAACCCGAAATGTATACATTGGTATATCTTGCAATTCGAATAACATCCTAAAAGTAGACGGTAAGCCTTCGAAACT
CAGGGACCTTGTTTCTGTCTTTAGCTGTGTAATATTCTCCCCAGAGGATATAGATCTAGTAAAGGGAGATCCCGGACACA
GAAGAAAATATCTGGATGATATTATCTGCAGGGCTCGCCCCATGATGCTGGACATTTATTCTGCATATGACAGAACATTA
AAGCAGAGAAACTCTCTGTTAAAGAGCTTTAGAAAATCATCATGCAAAAGCGACCTCCTGGATATTTGGACACAGAAGTT
GGTGGAGCTTGGCCTGGAAATAGTAAATGCTCGAAAAAGATTACTAAAGATTTTAAATCCAAAAATTAGCGAATTTTATT
CAAAACTTGCGGGGGTGAGTAGCACAGCTGAATTATTCTGCCAATCTTCAGATTGCCTTATAGATACATTCGATCTATTG
AGAGACAGAGAAATAGAAGAAGGTGTAACCCTTGCTGGACCACATAGAGATAACGTTGATATTCTCTTAAATTCTTGTCC
GGCAAGAAGTCAATCAAGCCAGGGAGAATCATGGACCCTTGCGCTGTCTATGAAACTTTCCCTTATAGAGCTTATGAGGG
AAACTAAAAGACTTTACGACCCAGATCCTGTTGTGATACTCGATGATGTCTTTGCTCATCTTGATTCATATCGAAAACAA
AAACTCGCACAGGAAGTGTTTAGCTATGAACAGACGATTGTAACAACAACCGATAATTCCGATAATTACAGAACATCTCA
AACCCTTGTGGTAGAAGAGGGAAAAGTGTTCAGCGAAAACAAAAAGGGGTAA

Upstream 100 bases:

>100_bases
AAGCCAAGCGTTAAAAATAGGCCAGGACCTGTTCTTATAACACCAAAATTATCCAATAAAGAAAAAATAGACTTTAAGTA
CCTTTTACAGCCAAATTTAT

Downstream 100 bases:

>100_bases
CTATCAATTCTAGAATTATAAGAAACTGTGTGAATCAGTACCTTAAAATCTCAAATACGGCAGGTTATTGTAGGCCAATT
CAGGATTTCAGCAGTAACCC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQNNLISHINLRNFRNYEYQSISFTDGLNLIRGDNGQGKTNLAEAIYFLSGFGSHRTYKNQPLIKSGEQKAEISAKIQSK
YGTRNVYIGISCNSNNILKVDGKPSKLRDLVSVFSCVIFSPEDIDLVKGDPGHRRKYLDDIICRARPMMLDIYSAYDRTL
KQRNSLLKSFRKSSCKSDLLDIWTQKLVELGLEIVNARKRLLKILNPKISEFYSKLAGVSSTAELFCQSSDCLIDTFDLL
RDREIEEGVTLAGPHRDNVDILLNSCPARSQSSQGESWTLALSMKLSLIELMRETKRLYDPDPVVILDDVFAHLDSYRKQ
KLAQEVFSYEQTIVTTTDNSDNYRTSQTLVVEEGKVFSENKKG

Sequences:

>Translated_363_residues
MQNNLISHINLRNFRNYEYQSISFTDGLNLIRGDNGQGKTNLAEAIYFLSGFGSHRTYKNQPLIKSGEQKAEISAKIQSK
YGTRNVYIGISCNSNNILKVDGKPSKLRDLVSVFSCVIFSPEDIDLVKGDPGHRRKYLDDIICRARPMMLDIYSAYDRTL
KQRNSLLKSFRKSSCKSDLLDIWTQKLVELGLEIVNARKRLLKILNPKISEFYSKLAGVSSTAELFCQSSDCLIDTFDLL
RDREIEEGVTLAGPHRDNVDILLNSCPARSQSSQGESWTLALSMKLSLIELMRETKRLYDPDPVVILDDVFAHLDSYRKQ
KLAQEVFSYEQTIVTTTDNSDNYRTSQTLVVEEGKVFSENKKG
>Mature_363_residues
MQNNLISHINLRNFRNYEYQSISFTDGLNLIRGDNGQGKTNLAEAIYFLSGFGSHRTYKNQPLIKSGEQKAEISAKIQSK
YGTRNVYIGISCNSNNILKVDGKPSKLRDLVSVFSCVIFSPEDIDLVKGDPGHRRKYLDDIICRARPMMLDIYSAYDRTL
KQRNSLLKSFRKSSCKSDLLDIWTQKLVELGLEIVNARKRLLKILNPKISEFYSKLAGVSSTAELFCQSSDCLIDTFDLL
RDREIEEGVTLAGPHRDNVDILLNSCPARSQSSQGESWTLALSMKLSLIELMRETKRLYDPDPVVILDDVFAHLDSYRKQ
KLAQEVFSYEQTIVTTTDNSDNYRTSQTLVVEEGKVFSENKKG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=373, Percent_Identity=24.6648793565684, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_TROW8 (Q83NZ4)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_788958.1
- ProteinModelPortal:   Q83NZ4
- STRING:   Q83NZ4
- GeneID:   1064354
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW003
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q83NZ4
- ProtClustDB:   CLSK229348
- BioCyc:   TWHI218496:TW0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41254; Mature: 41254

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNNLISHINLRNFRNYEYQSISFTDGLNLIRGDNGQGKTNLAEAIYFLSGFGSHRTYKN
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
QPLIKSGEQKAEISAKIQSKYGTRNVYIGISCNSNNILKVDGKPSKLRDLVSVFSCVIFS
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCC
PEDIDLVKGDPGHRRKYLDDIICRARPMMLDIYSAYDRTLKQRNSLLKSFRKSSCKSDLL
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIWTQKLVELGLEIVNARKRLLKILNPKISEFYSKLAGVSSTAELFCQSSDCLIDTFDLL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHH
RDREIEEGVTLAGPHRDNVDILLNSCPARSQSSQGESWTLALSMKLSLIELMRETKRLYD
HCCHHHCCCEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
PDPVVILDDVFAHLDSYRKQKLAQEVFSYEQTIVTTTDNSDNYRTSQTLVVEEGKVFSEN
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCCCC
KKG
CCC
>Mature Secondary Structure
MQNNLISHINLRNFRNYEYQSISFTDGLNLIRGDNGQGKTNLAEAIYFLSGFGSHRTYKN
CCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
QPLIKSGEQKAEISAKIQSKYGTRNVYIGISCNSNNILKVDGKPSKLRDLVSVFSCVIFS
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCC
PEDIDLVKGDPGHRRKYLDDIICRARPMMLDIYSAYDRTLKQRNSLLKSFRKSSCKSDLL
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIWTQKLVELGLEIVNARKRLLKILNPKISEFYSKLAGVSSTAELFCQSSDCLIDTFDLL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHH
RDREIEEGVTLAGPHRDNVDILLNSCPARSQSSQGESWTLALSMKLSLIELMRETKRLYD
HCCHHHCCCEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
PDPVVILDDVFAHLDSYRKQKLAQEVFSYEQTIVTTTDNSDNYRTSQTLVVEEGKVFSEN
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCCCC
KKG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174