Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 28572177

GI number: 28572177

Start: 1767

End: 2900

Strand: Direct

Name: dnaN [H]

Synonym: TW002

Alternate gene names: 28572177

Gene position: 1767-2900 (Clockwise)

Preceding gene: 28572176

Following gene: 28572178

Centisome position: 0.19

GC content: 37.74

Gene sequence:

>1134_bases
ATGAAGCTGATTGTCGATAGAGGTCTCCTTAGTTCCTCGGTAAATTTCGTAACCCGTATGATTCCTGCAAGACCTCCATC
ACCAATACTGTCTGGGATGTTAATTGAGGCAAAGAATGAGAAAGTTACTCTTTCAACTTTTAATTACGAGACATCAGCAA
AAGTCGAATTCAGCGCAAATGTAATAGAAGGCGGAAAGGTCTTAGTACCGGGAACTCTTATTAGTAGTATTTCGCAGAAA
TTGCCTGATGAACCAGTAGAGATAAGCAAAGAAGATGAAAAAATTTCTCTTACCTGCGGACCTGTAAATTACATACTTAA
TCTAATGCCGATAGCAGAGTATCCGCCACTACCTAACCTTGAGAATGCAGAGCAGTATAGGATACCTTCTGAAGATTTTG
TTAAATCAGTCTCGCAGGTTACAACATCGGCCGCAAAAGATGATATAAGCGCAGTTATAACAGGAATAAACCTAAGACTT
TCTAATGAATCAATTGAACTAACAGGAACAGACCGTTATAGAGTCGCGGTGAAGACCTTAAATAGCGTATCTGGACATCC
ATCTGATTCATCTGTTATTGTGTCGTCTAAAACCCTTTTTGAGGTTTCAAAAACGCTCGGGAATTTGGAAAGTGAAATAT
CAGTTTTTATAAAAAATGACGGAGAAAATAAAGTTATCGGATTTGAATCGGGAAATAAGACAGTAACGTCGCTCTTAATC
TCGGGGAATTATCCGCCAGTTGCAAAGCTATTTACAGAAGAAACAGGTCATTATGCAATCCTGAAAACAAGTGATTTCCT
GGAATCGACAAAAAGGGTCTCTGTCGTAGTTGAGAGAGATGAGCCAATTGAGTTTTCTTTTGTAAAAAATACAGTTACGA
TAAAAGGCTCAGGGATAGCACTTGCAAGTGAAAAAGTTGAGTGTGAACTATTTGGTGACGAAATAAGTATTATGTTGAGG
CCGCAGTTTTTATGCGACGGACTTATTGCTTGTCAAGAGGACTTTATAAAGGTTGCATTCACAAAGCCAAGCGTTAAAAA
TAGGCCAGGACCTGTTCTTATAACACCAAAATTATCCAATAAAGAAAAAATAGACTTTAAGTACCTTTTACAGCCAAATT
TATTTGCAAAATAA

Upstream 100 bases:

>100_bases
TTACGGGAGAATAATGCTTAAGCAGTAACTGAAACCTTAGTGTAAAAAAGCAAATAATTCATTAGATAGAATGAGACTCT
TCTGATCAGATTGGGAAAAT

Downstream 100 bases:

>100_bases
TTTGATTTCTCATATAAACCTAAGGAATTTTCGAAATTATGAGTACCAAAGTATTTCATTTACTGATGGTCTCAACCTTA
TAAGGGGTGATAATGGCCAG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKLIVDRGLLSSSVNFVTRMIPARPPSPILSGMLIEAKNEKVTLSTFNYETSAKVEFSANVIEGGKVLVPGTLISSISQK
LPDEPVEISKEDEKISLTCGPVNYILNLMPIAEYPPLPNLENAEQYRIPSEDFVKSVSQVTTSAAKDDISAVITGINLRL
SNESIELTGTDRYRVAVKTLNSVSGHPSDSSVIVSSKTLFEVSKTLGNLESEISVFIKNDGENKVIGFESGNKTVTSLLI
SGNYPPVAKLFTEETGHYAILKTSDFLESTKRVSVVVERDEPIEFSFVKNTVTIKGSGIALASEKVECELFGDEISIMLR
PQFLCDGLIACQEDFIKVAFTKPSVKNRPGPVLITPKLSNKEKIDFKYLLQPNLFAK

Sequences:

>Translated_377_residues
MKLIVDRGLLSSSVNFVTRMIPARPPSPILSGMLIEAKNEKVTLSTFNYETSAKVEFSANVIEGGKVLVPGTLISSISQK
LPDEPVEISKEDEKISLTCGPVNYILNLMPIAEYPPLPNLENAEQYRIPSEDFVKSVSQVTTSAAKDDISAVITGINLRL
SNESIELTGTDRYRVAVKTLNSVSGHPSDSSVIVSSKTLFEVSKTLGNLESEISVFIKNDGENKVIGFESGNKTVTSLLI
SGNYPPVAKLFTEETGHYAILKTSDFLESTKRVSVVVERDEPIEFSFVKNTVTIKGSGIALASEKVECELFGDEISIMLR
PQFLCDGLIACQEDFIKVAFTKPSVKNRPGPVLITPKLSNKEKIDFKYLLQPNLFAK
>Mature_377_residues
MKLIVDRGLLSSSVNFVTRMIPARPPSPILSGMLIEAKNEKVTLSTFNYETSAKVEFSANVIEGGKVLVPGTLISSISQK
LPDEPVEISKEDEKISLTCGPVNYILNLMPIAEYPPLPNLENAEQYRIPSEDFVKSVSQVTTSAAKDDISAVITGINLRL
SNESIELTGTDRYRVAVKTLNSVSGHPSDSSVIVSSKTLFEVSKTLGNLESEISVFIKNDGENKVIGFESGNKTVTSLLI
SGNYPPVAKLFTEETGHYAILKTSDFLESTKRVSVVVERDEPIEFSFVKNTVTIKGSGIALASEKVECELFGDEISIMLR
PQFLCDGLIACQEDFIKVAFTKPSVKNRPGPVLITPKLSNKEKIDFKYLLQPNLFAK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=257, Percent_Identity=24.124513618677, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41260; Mature: 41260

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVDRGLLSSSVNFVTRMIPARPPSPILSGMLIEAKNEKVTLSTFNYETSAKVEFSAN
CEEEEECCCHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCEEEEEEECCCCCEEEEEEEE
VIEGGKVLVPGTLISSISQKLPDEPVEISKEDEKISLTCGPVNYILNLMPIAEYPPLPNL
EECCCEEEECHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHEECCCCCCCCCC
ENAEQYRIPSEDFVKSVSQVTTSAAKDDISAVITGINLRLSNESIELTGTDRYRVAVKTL
CCCHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCEEEEECCCCEEEEEEEH
NSVSGHPSDSSVIVSSKTLFEVSKTLGNLESEISVFIKNDGENKVIGFESGNKTVTSLLI
HHCCCCCCCCEEEEECHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEECCCCEEEEEEE
SGNYPPVAKLFTEETGHYAILKTSDFLESTKRVSVVVERDEPIEFSFVKNTVTIKGSGIA
ECCCCCHHHHHHCCCCCEEEEECHHHHHCCCEEEEEEECCCCEEEEEEEEEEEEECCCEE
LASEKVECELFGDEISIMLRPQFLCDGLIACQEDFIKVAFTKPSVKNRPGPVLITPKLSN
EECCCEEEEEECCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCC
KEKIDFKYLLQPNLFAK
CCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKLIVDRGLLSSSVNFVTRMIPARPPSPILSGMLIEAKNEKVTLSTFNYETSAKVEFSAN
CEEEEECCCHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCEEEEEEECCCCCEEEEEEEE
VIEGGKVLVPGTLISSISQKLPDEPVEISKEDEKISLTCGPVNYILNLMPIAEYPPLPNL
EECCCEEEECHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHEECCCCCCCCCC
ENAEQYRIPSEDFVKSVSQVTTSAAKDDISAVITGINLRLSNESIELTGTDRYRVAVKTL
CCCHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCEEEEECCCCEEEEEEEH
NSVSGHPSDSSVIVSSKTLFEVSKTLGNLESEISVFIKNDGENKVIGFESGNKTVTSLLI
HHCCCCCCCCEEEEECHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEECCCCEEEEEEE
SGNYPPVAKLFTEETGHYAILKTSDFLESTKRVSVVVERDEPIEFSFVKNTVTIKGSGIA
ECCCCCHHHHHHCCCCCEEEEECHHHHHCCCEEEEEEECCCCEEEEEEEEEEEEECCCEE
LASEKVECELFGDEISIMLRPQFLCDGLIACQEDFIKVAFTKPSVKNRPGPVLITPKLSN
EECCCEEEEEECCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCC
KEKIDFKYLLQPNLFAK
CCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1577691; 12000953 [H]