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The map label for this gene is ligA [H]

Identifier: 326424055

GI number: 326424055

Start: 2702213

End: 2703055

Strand: Reverse

Name: ligA [H]

Synonym: NA

Alternate gene names: 326424055

Gene position: 2703055-2702213 (Counterclockwise)

Preceding gene: 27365968

Following gene: 326424054

Centisome position: 82.36

GC content: 48.4

Gene sequence:

>843_bases
ATGCGTTTGACTATATTATCGATGGCGATTCTCGTCGCCATCCCTGTGATGGCTGAGGAAGAGCCATTGCCAATACCAGA
GGTGATGCTTGCACAAACTTATGGGCATGGAGTGAAGATCAACGATTATTGGTATAGCGAAAAATTGGATGGTATTCGGG
CGTATTGGACTGGCACTCAATTGCTTACTCGCAATGGCAATCGAATTTATGCTCCGACGTGGTTTATTGAAGCATTACCA
CCGATGGCGCTCGATGGTGAGTTATGGGCCGGACGAGGTAATTTTCACATTGTGCAGCAGACAGTGCTGGACGCCACGCC
CGATGAGCATGCTTGGAAGCAGATTGACTTTATGGTTTTTGACCTGCCAGAGTCAGCGGGTGATTACCAAAAGCGCTACT
ATACCCTGATCGATTTAGTGAAAAACATCGACGCGAGGCACATCAAATTTGTTGAGCACAAACCGATCGTCAGCGAAGCA
CAGCTTTTTGAAAAACTGGATGCCATTACGGGCATCAGTGGTGAAGGGGTGATGCTTAGGAATATCACCAGCCGCTACCA
AGCGGGGCGGGGCAATGATCTACTGAAACTGAAGAAGTACAGTGATGCAGAAGCGACGGTCATTGGTTATAAGCCAGGGA
GTGGCCGTTTAATGGGTATGCTAGGCGCATTGTTGGTGCGGCTCGATGATGGTACCGAGTTCTATATCGGTAGTGGTTTC
ACCGACGAACAACGCAAAAATCCCCCTGCTATTGGTGCGACTGTTACTTATCGACACAATGGTTTCACTAACAGCGGCAA
ACCCAAATTTGCCCGATATCTGCGAGAGCGTCTTCCAGAATAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:
NA

Sequences:

>Translated_280_residues
MRLTILSMAILVAIPVMAEEEPLPIPEVMLAQTYGHGVKINDYWYSEKLDGIRAYWTGTQLLTRNGNRIYAPTWFIEALP
PMALDGELWAGRGNFHIVQQTVLDATPDEHAWKQIDFMVFDLPESAGDYQKRYYTLIDLVKNIDARHIKFVEHKPIVSEA
QLFEKLDAITGISGEGVMLRNITSRYQAGRGNDLLKLKKYSDAEATVIGYKPGSGRLMGMLGALLVRLDDGTEFYIGSGF
TDEQRKNPPAIGATVTYRHNGFTNSGKPKFARYLRERLPE
>Mature_280_residues
MRLTILSMAILVAIPVMAEEEPLPIPEVMLAQTYGHGVKINDYWYSEKLDGIRAYWTGTQLLTRNGNRIYAPTWFIEALP
PMALDGELWAGRGNFHIVQQTVLDATPDEHAWKQIDFMVFDLPESAGDYQKRYYTLIDLVKNIDARHIKFVEHKPIVSEA
QLFEKLDAITGISGEGVMLRNITSRYQAGRGNDLLKLKKYSDAEATVIGYKPGSGRLMGMLGALLVRLDDGTEFYIGSGF
TDEQRKNPPAIGATVTYRHNGFTNSGKPKFARYLRERLPE

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 31603; Mature: 31603

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00333 DNA_LIGASE_A2 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTILSMAILVAIPVMAEEEPLPIPEVMLAQTYGHGVKINDYWYSEKLDGIRAYWTGTQ
CEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEECEECCCCCCCEEEEEECCE
LLTRNGNRIYAPTWFIEALPPMALDGELWAGRGNFHIVQQTVLDATPDEHAWKQIDFMVF
EEECCCCEEECCEEHECCCCCCEECCCEEECCCCEEEEEEHHHCCCCCHHHHHEEEEEEE
DLPESAGDYQKRYYTLIDLVKNIDARHIKFVEHKPIVSEAQLFEKLDAITGISGEGVMLR
ECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
NITSRYQAGRGNDLLKLKKYSDAEATVIGYKPGSGRLMGMLGALLVRLDDGTEFYIGSGF
CHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCEEEEECCC
TDEQRKNPPAIGATVTYRHNGFTNSGKPKFARYLRERLPE
CCHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRLTILSMAILVAIPVMAEEEPLPIPEVMLAQTYGHGVKINDYWYSEKLDGIRAYWTGTQ
CEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEECEECCCCCCCEEEEEECCE
LLTRNGNRIYAPTWFIEALPPMALDGELWAGRGNFHIVQQTVLDATPDEHAWKQIDFMVF
EEECCCCEEECCEEHECCCCCCEECCCEEECCCCEEEEEEHHHCCCCCHHHHHEEEEEEE
DLPESAGDYQKRYYTLIDLVKNIDARHIKFVEHKPIVSEAQLFEKLDAITGISGEGVMLR
ECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
NITSRYQAGRGNDLLKLKKYSDAEATVIGYKPGSGRLMGMLGALLVRLDDGTEFYIGSGF
CHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCEEEEECCC
TDEQRKNPPAIGATVTYRHNGFTNSGKPKFARYLRERLPE
CCHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]