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The map label for this gene is ycdT [H]

Identifier: 326424054

GI number: 326424054

Start: 2700042

End: 2701961

Strand: Reverse

Name: ycdT [H]

Synonym: NA

Alternate gene names: 326424054

Gene position: 2701961-2700042 (Counterclockwise)

Preceding gene: 326424055

Following gene: 27365945

Centisome position: 82.33

GC content: 45.36

Gene sequence:

>1920_bases
ATGTCATCAAAAGCTAAACCGTTATTCATATCACTTTTTTCTGCTCTCATTCCAACTGTGGTTTTTGCCGAGCCTCATGC
TTTACCTTGCGATCTCTACTTAGATGAGTTCATCTCCGAGCAAGATCACATTTACCTTCAGTACTACGTGGATTCGCTAC
GCTACCCACAAATCACGGCAAATAAAGCGTTCGTTTATCCAGAAAATGGCAGTGATAACGCCAAAGCCTTGTATTTGTTT
GCTAAAAACAATCTCTTTAAGTACGAAGGAAAAGAAAGCTCCAACTTACCCGAAGTGCTTGAGTTGGCAGAGCAAACGGA
TCAGCAATGGATTAAGGCTGAAGCGAAGTTAACGCAAGGTCGCGGGCTGATGACCCAAAATCAGTTTCGCCAAGCAGAGG
CGTATTTTCTTGAAGCGCAAGAGATGGCAAATGCGATTGGCTACCAGTCTTTAGAAGGGCGCGCGCTAAAATGGCTCGGC
AATGTAGCCTATGAGTTTACCGATTTTACGGTGGCGCTGAATCAGTATCATCGTGCGTTAGAGATTTTTGAACAGAATGA
AGAGCCGCTTCAGGCTTCTATGGTGCTCAATAATATCTCCACTGTGTACATGGATAGCAAAGATTGGCAACGTGCGGAGC
AGTTCAATCGTCAGTCTAATCGACTCTATGACTCCAATCATTTCAAAAACACTTTGTTTGAAGCCATCTTACTCACGAAT
GCCAGTGTGATTGATGGAGCCATGGGACGTAAAGAGTCTGAGCTAAAAAACATCAGATTGGCATATGAAAAGGCCGGCGT
GGCAGGGGCCAGCTATCTAAAAATTAAAACCCTAGTTAACATGACGGAAGTATTGCTGAGGCAAGAGCACACGCAACTCG
CCATGGAAACAGGTTTAGAATGTCTCAAATTTTCACAGCAAAACGGCACTGAGAGTCAAATTGCTCATTGCCAAGACATT
CTTGCGGAAGTGTTCCTTGTCGAAAATGAACTCAAAGCTGCCACCAGCTATATCCAGTTGGCATTGAAGCAGTTTGAAGA
AGATAAAGAGACCATTGCGATCATCGAAGCGCTAAAAACATTGTCGCGAATTCAACAGCAATCGGGTGATCTCAAGTCTG
CACTGCAAACTTATCAAACCGCGTCGCAGTTAGAGCAAAAATACCTAACCGAATCGTTTAACTCTGACGTGGCGATTGTC
AGAGGTCGGCTCGATGAAAAACTCGCTTCGCAAGAGCTGATGTTGCTTGAGGCGCAAAACGCCTTGCAATTGACCCAACT
GGAAGCGCAAAAATTACGTGAATGGGCCTATATGCTGGCCGTAACTCTATTCTCGTTTCTCGCATATCTGCTTTGGCGTA
AGCTCAAAGCAAGCCAACGTTTACAACAAGCATTGACGCAACAAAACGTGCAACTAGAGACGCAATCTATGATTGATCCT
CTCACGCGGGTCAACAATCGCAGCTATCTGGATAAATGGTGGCAAAGCATCAGCAACAAGTCAGACACCATGAACACGCC
GAAATGCTTGGCCGTAGTTGATATCGATCATTTCAAACAGATCAATGATACCTATGGACATCAAGTGGGGGATGATGTGC
TGTCAAAAGTGGCCGACATGCTTAGCTGCTGTACACGCGGTGACGACTTACTGCTTCGATGGGGGGGAGAAGAGTTTGTT
TTGGTATTGAATCTAACTTGCCAACAGGCCCATCAGACATTAACTAGGCTGCAAGAAGCGATTTCGCGCACTGAAATTGC
CGCGTCGGATGAGGTTGTCGCCGTTACGGTTTCGATTGGTGCGGTTTGTGTCTCTTCGGCTCATGAATTGCAGCAAAATT
GGGAACCCTATTTATTTCAAGCCGATGAAGCGTTATATGACATCAAACGCGGTGGCAGAAATGGCGTAAAAGTCATCTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:
NA

Sequences:

>Translated_639_residues
MSSKAKPLFISLFSALIPTVVFAEPHALPCDLYLDEFISEQDHIYLQYYVDSLRYPQITANKAFVYPENGSDNAKALYLF
AKNNLFKYEGKESSNLPEVLELAEQTDQQWIKAEAKLTQGRGLMTQNQFRQAEAYFLEAQEMANAIGYQSLEGRALKWLG
NVAYEFTDFTVALNQYHRALEIFEQNEEPLQASMVLNNISTVYMDSKDWQRAEQFNRQSNRLYDSNHFKNTLFEAILLTN
ASVIDGAMGRKESELKNIRLAYEKAGVAGASYLKIKTLVNMTEVLLRQEHTQLAMETGLECLKFSQQNGTESQIAHCQDI
LAEVFLVENELKAATSYIQLALKQFEEDKETIAIIEALKTLSRIQQQSGDLKSALQTYQTASQLEQKYLTESFNSDVAIV
RGRLDEKLASQELMLLEAQNALQLTQLEAQKLREWAYMLAVTLFSFLAYLLWRKLKASQRLQQALTQQNVQLETQSMIDP
LTRVNNRSYLDKWWQSISNKSDTMNTPKCLAVVDIDHFKQINDTYGHQVGDDVLSKVADMLSCCTRGDDLLLRWGGEEFV
LVLNLTCQQAHQTLTRLQEAISRTEIAASDEVVAVTVSIGAVCVSSAHELQQNWEPYLFQADEALYDIKRGGRNGVKVI
>Mature_638_residues
SSKAKPLFISLFSALIPTVVFAEPHALPCDLYLDEFISEQDHIYLQYYVDSLRYPQITANKAFVYPENGSDNAKALYLFA
KNNLFKYEGKESSNLPEVLELAEQTDQQWIKAEAKLTQGRGLMTQNQFRQAEAYFLEAQEMANAIGYQSLEGRALKWLGN
VAYEFTDFTVALNQYHRALEIFEQNEEPLQASMVLNNISTVYMDSKDWQRAEQFNRQSNRLYDSNHFKNTLFEAILLTNA
SVIDGAMGRKESELKNIRLAYEKAGVAGASYLKIKTLVNMTEVLLRQEHTQLAMETGLECLKFSQQNGTESQIAHCQDIL
AEVFLVENELKAATSYIQLALKQFEEDKETIAIIEALKTLSRIQQQSGDLKSALQTYQTASQLEQKYLTESFNSDVAIVR
GRLDEKLASQELMLLEAQNALQLTQLEAQKLREWAYMLAVTLFSFLAYLLWRKLKASQRLQQALTQQNVQLETQSMIDPL
TRVNNRSYLDKWWQSISNKSDTMNTPKCLAVVDIDHFKQINDTYGHQVGDDVLSKVADMLSCCTRGDDLLLRWGGEEFVL
VLNLTCQQAHQTLTRLQEAISRTEIAASDEVVAVTVSIGAVCVSSAHELQQNWEPYLFQADEALYDIKRGGRNGVKVI

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=40.4907975460123, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=34.3558282208589, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1786584, Length=239, Percent_Identity=27.6150627615063, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=128, Percent_Identity=39.0625, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=205, Percent_Identity=32.6829268292683, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787816, Length=173, Percent_Identity=32.9479768786127, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=30, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=29.4416243654822, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=156, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787802, Length=166, Percent_Identity=31.3253012048193, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=102, Percent_Identity=38.2352941176471, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 72691; Mature: 72560

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKAKPLFISLFSALIPTVVFAEPHALPCDLYLDEFISEQDHIYLQYYVDSLRYPQITA
CCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEEEC
NKAFVYPENGSDNAKALYLFAKNNLFKYEGKESSNLPEVLELAEQTDQQWIKAEAKLTQG
CEEEEECCCCCCCCEEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RGLMTQNQFRQAEAYFLEAQEMANAIGYQSLEGRALKWLGNVAYEFTDFTVALNQYHRAL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIFEQNEEPLQASMVLNNISTVYMDSKDWQRAEQFNRQSNRLYDSNHFKNTLFEAILLTN
HHHHCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
ASVIDGAMGRKESELKNIRLAYEKAGVAGASYLKIKTLVNMTEVLLRQEHTQLAMETGLE
CHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
CLKFSQQNGTESQIAHCQDILAEVFLVENELKAATSYIQLALKQFEEDKETIAIIEALKT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRIQQQSGDLKSALQTYQTASQLEQKYLTESFNSDVAIVRGRLDEKLASQELMLLEAQN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
ALQLTQLEAQKLREWAYMLAVTLFSFLAYLLWRKLKASQRLQQALTQQNVQLETQSMIDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LTRVNNRSYLDKWWQSISNKSDTMNTPKCLAVVDIDHFKQINDTYGHQVGDDVLSKVADM
HHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHH
LSCCTRGDDLLLRWGGEEFVLVLNLTCQQAHQTLTRLQEAISRTEIAASDEVVAVTVSIG
HHHHCCCCCEEEEECCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHH
AVCVSSAHELQQNWEPYLFQADEALYDIKRGGRNGVKVI
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSKAKPLFISLFSALIPTVVFAEPHALPCDLYLDEFISEQDHIYLQYYVDSLRYPQITA
CCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEEEC
NKAFVYPENGSDNAKALYLFAKNNLFKYEGKESSNLPEVLELAEQTDQQWIKAEAKLTQG
CEEEEECCCCCCCCEEEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RGLMTQNQFRQAEAYFLEAQEMANAIGYQSLEGRALKWLGNVAYEFTDFTVALNQYHRAL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIFEQNEEPLQASMVLNNISTVYMDSKDWQRAEQFNRQSNRLYDSNHFKNTLFEAILLTN
HHHHCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
ASVIDGAMGRKESELKNIRLAYEKAGVAGASYLKIKTLVNMTEVLLRQEHTQLAMETGLE
CHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
CLKFSQQNGTESQIAHCQDILAEVFLVENELKAATSYIQLALKQFEEDKETIAIIEALKT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRIQQQSGDLKSALQTYQTASQLEQKYLTESFNSDVAIVRGRLDEKLASQELMLLEAQN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
ALQLTQLEAQKLREWAYMLAVTLFSFLAYLLWRKLKASQRLQQALTQQNVQLETQSMIDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LTRVNNRSYLDKWWQSISNKSDTMNTPKCLAVVDIDHFKQINDTYGHQVGDDVLSKVADM
HHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHH
LSCCTRGDDLLLRWGGEEFVLVLNLTCQQAHQTLTRLQEAISRTEIAASDEVVAVTVSIG
HHHHCCCCCEEEEECCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHH
AVCVSSAHELQQNWEPYLFQADEALYDIKRGGRNGVKVI
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]