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The map label for this gene is prfA [H]

Identifier: 326423672

GI number: 326423672

Start: 246021

End: 247109

Strand: Reverse

Name: prfA [H]

Synonym: NA

Alternate gene names: 326423672

Gene position: 247109-246021 (Counterclockwise)

Preceding gene: 27363735

Following gene: 27363733

Centisome position: 7.53

GC content: 49.4

Gene sequence:

>1089_bases
ATGAAAGCCTCGATTCTAACCAAGCTAGAAATGCTGGTTGAGCGTTATGAAGAAGTGCAGCACCTTCTTGGTGATCCGGG
AGTGATCGGTGACCAAGACAAATTCCGTGCACTGTCAAAAGAGTATTCTCAACTTGAAGAAGTCACTAAGTGCTTTACTG
CCTATAAACAAGCGCAAGAAGATTTAGTTGCTGCGGAAGAGATGGCCAAAGAAGACGACGCAGAGATGCGTGAAATGGCG
CAAGAAGAAATCAAAGCGGCAAAAGTGGCCATTGAAGATTTGGCTGCAGAGTTGCAAATTCTTCTGCTACCGAAAGATCC
GAATGACGATCGCAACTGTTTCCTCGAAATTCGTGCGGGCGCAGGCGGTGATGAAGCCGGTATCTTTGCTGGTGATCTGT
TCCGTATGTACAGCAAGTTTGCAGAAAAACGTGGTTGGCGTATTGAAGTCATGTCCGCGAATGAAGCAGAGCATGGCGGC
TACAAAGAGATGATCGCGAAAGTGAATGGCGATGGCGCTTACGGTTTCTTGAAGTTTGAATCGGGCGGTCACCGTGTTCA
ACGTGTTCCTGCAACCGAATCGCAAGGTCGCGTACACACCTCGGCGTGTACCGTGGCAATCATGCCTGAAATTCCTGAAG
CGGAAATTCCAGAGATCAAAGCAAGCGATCTAAAAATTGATACCTTCCGCTCTTCTGGCGCGGGCGGTCAGCACGTTAAC
ACCACCGACTCGGCAATCCGTATTACTCACTTACCAACCGGTACTGTCGTGGAGTGTCAGGATGAGCGTTCACAGCACAA
AAACAAAGCCAAGGCGATGGCTGTTCTAGCGGCTCGTATTGTTCAAGCTGAAGAGGCTAAGCGTGCAGCTGAAGTGTCTG
ATACGCGTCGTAACCTGCTTGGTTCGGGCGATCGTAGTGACCGTATTCGTACCTACAACTACCCACAAGGCCGTGTGTCA
GATCACCGCATCAACTTGACGATTTACCGTCTTTCTGAAGTGATGGAAGGCGATCTTCAGTCTTTGATCGATCCTGTGAT
TCAGGAACACCAAGCGGACCAATTGGCTGCTTTGGCGGAAAACAACTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:
NA

Sequences:

>Translated_362_residues
MKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQEDLVAAEEMAKEDDAEMREMA
QEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHGG
YKEMIAKVNGDGAYGFLKFESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAENN
>Mature_362_residues
MKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQEDLVAAEEMAKEDDAEMREMA
QEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHGG
YKEMIAKVNGDGAYGFLKFESGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAENN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=43.3774834437086, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=35.7340720221607, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=42.512077294686, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=72.7777777777778, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=38.7573964497041, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=290, Percent_Identity=35.5172413793103, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=332, Percent_Identity=39.7590361445783, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=40, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40139; Mature: 40139

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAEEMAKEDDAEMREMAQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHGGYKEMIAKVNGDGAYGFLKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEC
SGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
CCCCEEEECCCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAE
CCCCCCCCCEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
NN
CC
>Mature Secondary Structure
MKASILTKLEMLVERYEEVQHLLGDPGVIGDQDKFRALSKEYSQLEEVTKCFTAYKQAQE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAEEMAKEDDAEMREMAQEEIKAAKVAIEDLAAELQILLLPKDPNDDRNCFLEIRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGDLFRMYSKFAEKRGWRIEVMSANEAEHGGYKEMIAKVNGDGAYGFLKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEC
SGGHRVQRVPATESQGRVHTSACTVAIMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
CCCCEEEECCCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TTDSAIRITHLPTGTVVECQDERSQHKNKAKAMAVLAARIVQAEEAKRAAEVSDTRRNLL
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTIYRLSEVMEGDLQSLIDPVIQEHQADQLAALAE
CCCCCCCCCEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
NN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA