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The map label for this gene is menE [H]

Identifier: 27366429

GI number: 27366429

Start: 3241350

End: 3242780

Strand: Reverse

Name: menE [H]

Synonym: VV1_3168

Alternate gene names: 27366429

Gene position: 3242780-3241350 (Counterclockwise)

Preceding gene: 27366430

Following gene: 326424146

Centisome position: 98.81

GC content: 50.31

Gene sequence:

>1431_bases
ATGGGTGAAACGGCATTGGTCAGTGAACAACCATTGTGGAAATATTGGGCTCAGAAGAGCCCATTTTCTATGGCGCTGAA
CTCATCACGTTATCAATTCAACTGGCAACAACTGAACGACGAGATTGAACGTTTAGCGGCGGTTCTTGGTGCGCAAGGGG
TGACTCGTGGAGATGTCATCACTCTGCTGGGTAAAAACCATGTTGATATGGTGCTTATCTTGCTCGCTGCGCATCAACTG
GGGGCTGTGGTGGCGATCATCATGCCGCAATCAATTGCAATGTTCTCAGCAAAGCTTGATACGCTTTACCGTGAGCAGCA
AACGCGCTTTATTTGGCTTGATCCTAACGTTGATGTGCAGACCCGCTCGGCTTGCTCTCACGATCGTCGTAATGTACTGC
TAGAAATCTCACTCGCCTCGGATCATCTTAACTCGATGCCTAGCAACCACGTTTGCGATTATCGCGCCGATCAACTTGCT
ACCATCATTTTTACCTCTGGTTCCACGGGCAATCCCAAAGCGGTGGCTCATACTCATCGGCAGCATTTCGCGTCAGCACA
AGGATTGTTAGAAGAGTTCTGTTTTACTCAGCAGGATACGTGGTTGCTCTCCCTGCCGCTGTATCATGTTTCTGGCATGG
CTATTGTTTATCGCTGGTTGTTGGCCGGCGCATGTTTGAAAGTGGGGCATGGCGCTTTGTCTGAGGACATTCAAGGGGTG
ACACACGCGTCTCTCGTCGCGACTCAGTTGAAGCGCCTGCTTGATGACAACACGCCTTTGCAACTGAGCCATGTTTTGCT
GGGGGGGAGTCATGTGGAGCATGCCTTGGCCCGAGCGGCGCAGCGTAAAGGAATAGAGACCTGGCTAGGCTATGGCATGA
CAGAGGCCGCTTCAACCGTGACCGCCAAGCAGATCGACAGCGTGAGCAACGCAGGGCATCTATTGAAAAATCGAGCACTG
CAACTGATTGGTCAGCGCATTTATATCGGTGGTGAAACCTTGGCAGAAGGCTATTTCTTTCAGGGTAGCCTAACGACGCT
GGTGGATGAGCATGGGTGGTTTGACAGTAAAGATTTAGGCGTCTGGCAAGGTGAGGAACTTAAGATCATCGGCCGTGCGG
ACAATCAGTTTATCTCTGGCGGGGAAAACATCCACTGCGAAGAGATTGAAGCGGTGCTAAACGAGATTGAAGGCGTGGAA
CAGAGTATTGTGGTTCCCATGCCCGATGCAGAATTTGGCTATCGACCCGTTGCTGTCATCCAATCGCCATCGCTAGCAAA
CAGAGCGCAGTATGAGCAACACTTAAAAGAGAAACTGCAAAAGTTTAAGTGGCCAGTCGCCTATTACTTAATGCCTGAGG
CGTTACTGAAGGAAGGGATTAAAGTCTCGCGGCAAGCCGTTAAAGTATGGCTGGAAAATCAACTTAGTTGA

Upstream 100 bases:

>100_bases
ACACCATAAGTTTGTTTCAATCGCAGTTAATGGTGGGATGGCCGAAATGCTCGCTGCCGATCGCCTCGTTAGAAGAACAG
CAATTGGTGTGGCATTCATA

Downstream 100 bases:

>100_bases
TTTCGACATGAATTAGTCAAAAAGAGAATTTCTCAACCTTATAACCCTCAACAATAATTGCGCATCATATGACAAAGAAA
AGGCATAAGGTTGAAGATGG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MGETALVSEQPLWKYWAQKSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVTRGDVITLLGKNHVDMVLILLAAHQL
GAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQLA
TIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIVYRWLLAGACLKVGHGALSEDIQGV
THASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAASTVTAKQIDSVSNAGHLLKNRAL
QLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGVE
QSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPEALLKEGIKVSRQAVKVWLENQLS

Sequences:

>Translated_476_residues
MGETALVSEQPLWKYWAQKSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVTRGDVITLLGKNHVDMVLILLAAHQL
GAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQLA
TIIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIVYRWLLAGACLKVGHGALSEDIQGV
THASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAASTVTAKQIDSVSNAGHLLKNRAL
QLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGVE
QSIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPEALLKEGIKVSRQAVKVWLENQLS
>Mature_475_residues
GETALVSEQPLWKYWAQKSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVTRGDVITLLGKNHVDMVLILLAAHQLG
AVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQTRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQLAT
IIFTSGSTGNPKAVAHTHRQHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIVYRWLLAGACLKVGHGALSEDIQGVT
HASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAASTVTAKQIDSVSNAGHLLKNRALQ
LIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLGVWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGVEQ
SIVVPMPDAEFGYRPVAVIQSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPEALLKEGIKVSRQAVKVWLENQLS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=470, Percent_Identity=37.2340425531915, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI145693145, Length=493, Percent_Identity=23.9350912778905, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=438, Percent_Identity=24.8858447488584, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52919; Mature: 52788

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETALVSEQPLWKYWAQKSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVTRGDVI
CCCCCCCCCCCHHHHHCCCCCHHEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEE
TLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQ
EEECCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
TRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQLATIIFTSGSTGNPKAVAHTHR
CCHHHCCCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
QHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIVYRWLLAGACLKVGHGALSEDIQGV
HHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
THASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAASTV
HHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
TAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCEEECCCHHHHHHCCCCCCCCCCC
VWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGVEQSIVVPMPDAEFGYRPVAVI
CCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEE
QSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPEALLKEGIKVSRQAVKVWLENQLS
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GETALVSEQPLWKYWAQKSPFSMALNSSRYQFNWQQLNDEIERLAAVLGAQGVTRGDVI
CCCCCCCCCCHHHHHCCCCCHHEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEE
TLLGKNHVDMVLILLAAHQLGAVVAIIMPQSIAMFSAKLDTLYREQQTRFIWLDPNVDVQ
EEECCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
TRSACSHDRRNVLLEISLASDHLNSMPSNHVCDYRADQLATIIFTSGSTGNPKAVAHTHR
CCHHHCCCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
QHFASAQGLLEEFCFTQQDTWLLSLPLYHVSGMAIVYRWLLAGACLKVGHGALSEDIQGV
HHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
THASLVATQLKRLLDDNTPLQLSHVLLGGSHVEHALARAAQRKGIETWLGYGMTEAASTV
HHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
TAKQIDSVSNAGHLLKNRALQLIGQRIYIGGETLAEGYFFQGSLTTLVDEHGWFDSKDLG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCEEECCCHHHHHHCCCCCCCCCCC
VWQGEELKIIGRADNQFISGGENIHCEEIEAVLNEIEGVEQSIVVPMPDAEFGYRPVAVI
CCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEE
QSPSLANRAQYEQHLKEKLQKFKWPVAYYLMPEALLKEGIKVSRQAVKVWLENQLS
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]