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The map label for this gene is oppA [H]

Identifier: 27366351

GI number: 27366351

Start: 3150984

End: 3152615

Strand: Reverse

Name: oppA [H]

Synonym: VV1_3082

Alternate gene names: 27366351

Gene position: 3152615-3150984 (Counterclockwise)

Preceding gene: 27366352

Following gene: 27366350

Centisome position: 96.06

GC content: 46.2

Gene sequence:

>1632_bases
ATGTATAAGAACAAAATCACCCAAGCACTTCTTCTTGGTGCTGGTCTAACACTCGCAACTACCTCTCTTTCTGCTCTTGC
CGCAGACGTACCAGCTGGAACTAAATTAGCAGCAAAACAAGAACTCGTTCGTGGTAACGGTACTGAGGTTGCTACTATCG
ACCCGCACAAATCTCAAGGCGTGCCAGAGTCGCATGTAATTCGTGACCTATTGGAAGGTCTAGTAAACCAAGATGCAGAC
GGCAACACCATTCCTGGTGTCGCAACGAGCTGGGAAACGACGGACAACAAGACCTTTACTTTCCACCTACGTAAAGATGC
AAAATGGTCTAACGGCGACCCTGTTACCGCGCAAGATTTCGTATACAGCTGGCAACGTGCTGTCGATCCAGCAACAGCAT
CGCCATACTCTTGGTACATGGAATACACCAAGATGAAAAACGCGAAAGACATCGTTGCAGGGAAGAAAGACAAGTCTGAG
CTTGGTGTAAAAGCGGCTGACGATTACACGCTAGTGATTGAGCTTGAAACGGCGGTTCCTTACTTTGTGATGATGACTGG
CCACACCACCATGAAACCAGTACACAAAGCAACGGTAGAGAAGTTTGGTGATCAATGGACTAAGCCAGAAAACTTCGTCG
GTAACGGTGCGTTTGTTGTAGACAAATGGGTTGTTAACGAACGTTTAGAAATGGTTCGTAACACCAAGTACTGGGATGAC
TCTAAGACAGTGATCAACAAAGTCACTTTCCTACCAATCGAAAACGCGGTTGCAGAAATGAACCGTTTCCTTTCGGGTGA
AATTGACTTTACATCAACTCTACCAACTGAACACTTCAAGCGTCTGAAAAAAGAACACCCAGAATCGGTTTCTGTTGAAG
GCAGCCTATGTACTTACTACTACTCTTTCAACACGAAGAAAAAGCCGTTTGATGACGTACGTGTACGTAAAGCGATCTCT
TACGCGATTGACCGTGACATCGTAGCAAACGCGATCTTAGGTCAGGGTCAGAAGCCTGCGTATTTCCTAACGCCAGAGAT
CACTGCAAACTTCAACCCAGAACTTCCTGAGTACGGTAAAATGTCTCAGAAAGAGCGTAACGCAGAAGCGGCTCGCCTAC
TTGAAGAAGCGGGTTACGGCAAAGGCAACCCTCTGAAGTTTACTTTGCTTTACAACACCAACGAAAACCACAAGAAGATC
GCCGTTGCGCTCGGTTCTATGTGGAAGAAAACACTTGGTCTAGATGTTAATCTTGAAAACCAAGAGTGGAAAACTTACCT
ATCTACTAAAGATCAAGGTGATTTCGAAGTGGCACGCGCCGGTTGGTGTGGCGACTACAACGAAGCATCTTCGTTCCTAA
CTCTGATGGTGAGCGCGAATACAACGGGTGGTCAGCACTGGGGTAATGCAGAGTATGATGCTCTGATCGACAAAGCGCTG
AAGTCAACATCTGAAGCAGAGCGTACTGCGCTGTACTTGGATGCTGAAAATCTGATGGCAAAAGATATGCCTATCGCACC
AATCTACCAATATGTACGTTCGCGTCTACTAAATCCACGTGTTGGTGGTTTCCCATCAAACAATGCGGAAGAGAAGATCT
ACTCAAAAGATCTCTACATCATCGCGAAGTAA

Upstream 100 bases:

>100_bases
TTTTGTTTTCTAAAACGCAGCTAAAAAGTTCTAAAACACTGCCGTTCTGGTGTTTTGGCAAAGAAGTCATGGATGCAAAT
ATAAAACTTGGAGTTTATGC

Downstream 100 bases:

>100_bases
TCACCTAAGCTGATAACTATAAAAAATAGAGACACTGCATGTGGTATAGCCGCATGCAGCGTCTAATTTCATTTAAACTG
TCACAGACTGAAAGAGTGAG

Product: oligopeptide ABC transporter periplasmic oligopeptide-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MYKNKITQALLLGAGLTLATTSLSALAADVPAGTKLAAKQELVRGNGTEVATIDPHKSQGVPESHVIRDLLEGLVNQDAD
GNTIPGVATSWETTDNKTFTFHLRKDAKWSNGDPVTAQDFVYSWQRAVDPATASPYSWYMEYTKMKNAKDIVAGKKDKSE
LGVKAADDYTLVIELETAVPYFVMMTGHTTMKPVHKATVEKFGDQWTKPENFVGNGAFVVDKWVVNERLEMVRNTKYWDD
SKTVINKVTFLPIENAVAEMNRFLSGEIDFTSTLPTEHFKRLKKEHPESVSVEGSLCTYYYSFNTKKKPFDDVRVRKAIS
YAIDRDIVANAILGQGQKPAYFLTPEITANFNPELPEYGKMSQKERNAEAARLLEEAGYGKGNPLKFTLLYNTNENHKKI
AVALGSMWKKTLGLDVNLENQEWKTYLSTKDQGDFEVARAGWCGDYNEASSFLTLMVSANTTGGQHWGNAEYDALIDKAL
KSTSEAERTALYLDAENLMAKDMPIAPIYQYVRSRLLNPRVGGFPSNNAEEKIYSKDLYIIAK

Sequences:

>Translated_543_residues
MYKNKITQALLLGAGLTLATTSLSALAADVPAGTKLAAKQELVRGNGTEVATIDPHKSQGVPESHVIRDLLEGLVNQDAD
GNTIPGVATSWETTDNKTFTFHLRKDAKWSNGDPVTAQDFVYSWQRAVDPATASPYSWYMEYTKMKNAKDIVAGKKDKSE
LGVKAADDYTLVIELETAVPYFVMMTGHTTMKPVHKATVEKFGDQWTKPENFVGNGAFVVDKWVVNERLEMVRNTKYWDD
SKTVINKVTFLPIENAVAEMNRFLSGEIDFTSTLPTEHFKRLKKEHPESVSVEGSLCTYYYSFNTKKKPFDDVRVRKAIS
YAIDRDIVANAILGQGQKPAYFLTPEITANFNPELPEYGKMSQKERNAEAARLLEEAGYGKGNPLKFTLLYNTNENHKKI
AVALGSMWKKTLGLDVNLENQEWKTYLSTKDQGDFEVARAGWCGDYNEASSFLTLMVSANTTGGQHWGNAEYDALIDKAL
KSTSEAERTALYLDAENLMAKDMPIAPIYQYVRSRLLNPRVGGFPSNNAEEKIYSKDLYIIAK
>Mature_543_residues
MYKNKITQALLLGAGLTLATTSLSALAADVPAGTKLAAKQELVRGNGTEVATIDPHKSQGVPESHVIRDLLEGLVNQDAD
GNTIPGVATSWETTDNKTFTFHLRKDAKWSNGDPVTAQDFVYSWQRAVDPATASPYSWYMEYTKMKNAKDIVAGKKDKSE
LGVKAADDYTLVIELETAVPYFVMMTGHTTMKPVHKATVEKFGDQWTKPENFVGNGAFVVDKWVVNERLEMVRNTKYWDD
SKTVINKVTFLPIENAVAEMNRFLSGEIDFTSTLPTEHFKRLKKEHPESVSVEGSLCTYYYSFNTKKKPFDDVRVRKAIS
YAIDRDIVANAILGQGQKPAYFLTPEITANFNPELPEYGKMSQKERNAEAARLLEEAGYGKGNPLKFTLLYNTNENHKKI
AVALGSMWKKTLGLDVNLENQEWKTYLSTKDQGDFEVARAGWCGDYNEASSFLTLMVSANTTGGQHWGNAEYDALIDKAL
KSTSEAERTALYLDAENLMAKDMPIAPIYQYVRSRLLNPRVGGFPSNNAEEKIYSKDLYIIAK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=538, Percent_Identity=51.8587360594795, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI87081878, Length=528, Percent_Identity=50.3787878787879, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1789397, Length=517, Percent_Identity=43.9071566731141, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787762, Length=528, Percent_Identity=24.4318181818182, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789966, Length=467, Percent_Identity=24.4111349036403, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789887, Length=542, Percent_Identity=25.4612546125461, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787052, Length=497, Percent_Identity=24.1448692152917, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1787551, Length=518, Percent_Identity=22.5868725868726, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60674; Mature: 60674

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKNKITQALLLGAGLTLATTSLSALAADVPAGTKLAAKQELVRGNGTEVATIDPHKSQG
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCC
VPESHVIRDLLEGLVNQDADGNTIPGVATSWETTDNKTFTFHLRKDAKWSNGDPVTAQDF
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHH
VYSWQRAVDPATASPYSWYMEYTKMKNAKDIVAGKKDKSELGVKAADDYTLVIELETAVP
HHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHCCCEECCCEEEEEEEECCCC
YFVMMTGHTTMKPVHKATVEKFGDQWTKPENFVGNGAFVVDKWVVNERLEMVRNTKYWDD
EEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCC
SKTVINKVTFLPIENAVAEMNRFLSGEIDFTSTLPTEHFKRLKKEHPESVSVEGSLCTYY
CHHHHHEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEE
YSFNTKKKPFDDVRVRKAISYAIDRDIVANAILGQGQKPAYFLTPEITANFNPELPEYGK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCCCCC
MSQKERNAEAARLLEEAGYGKGNPLKFTLLYNTNENHKKIAVALGSMWKKTLGLDVNLEN
CHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCEEEECC
QEWKTYLSTKDQGDFEVARAGWCGDYNEASSFLTLMVSANTTGGQHWGNAEYDALIDKAL
HHHHHHHCCCCCCCEEEEECCCCCCHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
KSTSEAERTALYLDAENLMAKDMPIAPIYQYVRSRLLNPRVGGFPSNNAEEKIYSKDLYI
HCCCHHHHEEEEEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEE
IAK
EEC
>Mature Secondary Structure
MYKNKITQALLLGAGLTLATTSLSALAADVPAGTKLAAKQELVRGNGTEVATIDPHKSQG
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCC
VPESHVIRDLLEGLVNQDADGNTIPGVATSWETTDNKTFTFHLRKDAKWSNGDPVTAQDF
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHH
VYSWQRAVDPATASPYSWYMEYTKMKNAKDIVAGKKDKSELGVKAADDYTLVIELETAVP
HHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHCCCEECCCEEEEEEEECCCC
YFVMMTGHTTMKPVHKATVEKFGDQWTKPENFVGNGAFVVDKWVVNERLEMVRNTKYWDD
EEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCC
SKTVINKVTFLPIENAVAEMNRFLSGEIDFTSTLPTEHFKRLKKEHPESVSVEGSLCTYY
CHHHHHEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEE
YSFNTKKKPFDDVRVRKAISYAIDRDIVANAILGQGQKPAYFLTPEITANFNPELPEYGK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCCCCC
MSQKERNAEAARLLEEAGYGKGNPLKFTLLYNTNENHKKIAVALGSMWKKTLGLDVNLEN
CHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCEEEECC
QEWKTYLSTKDQGDFEVARAGWCGDYNEASSFLTLMVSANTTGGQHWGNAEYDALIDKAL
HHHHHHHCCCCCCCEEEEECCCCCCHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
KSTSEAERTALYLDAENLMAKDMPIAPIYQYVRSRLLNPRVGGFPSNNAEEKIYSKDLYI
HCCCHHHHEEEEEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEE
IAK
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]