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The map label for this gene is rluB

Identifier: 27366329

GI number: 27366329

Start: 3131143

End: 3132105

Strand: Reverse

Name: rluB

Synonym: VV1_3060

Alternate gene names: 27366329

Gene position: 3132105-3131143 (Counterclockwise)

Preceding gene: 27366331

Following gene: 27366326

Centisome position: 95.44

GC content: 49.12

Gene sequence:

>963_bases
ATGAACGAAAAATTACAGAAAGTTTTGGCACGAGCTGGTCATGGCTCACGTCGTGAATTGGAAGCATTAATCAAATCAGG
ACGTGTGAGTGTGAACGGTATCGTCGCGAAACTGGGCGAACGACTAGAAGATGAGCAAGCAGTAGTGCGCATCGATGGTC
ACGTTGTGTCCGTGAAAGCTCAGGAAGAGGTGATTTGCCGAGTGTTGGCATACTACAAACCTGAAGGTGAGCTCTGTACG
CGTCACGACCCAGAAGGTCGCCGTACTGTTTTTGATCGTCTACCAAAAATCCGTGGCTCTCGCTGGATCTCTGTTGGTCG
TCTTGATGCAAACACCTCTGGTTTGTTGCTTTTTACCACTGATGGTGAATTGGCAAATCGCCTAATGCACCCAAGTCGTC
AAGTTGAACGTGAATACCTAGTTCGCGTGTTTGGCGAAGTGAATGAGCACAAGGTACGTAACCTCGTTAACGGCGTTGAG
CTCGATGATGGTTTAGCTCGCTTTGAAGACGTGGTTTATACTGGCGGTGAAGGTATGAACCATACGTTTTACGTGGTGAT
CAATGAAGGTCGTAACCGTGAGGTTCGTCGTCTTTGGGAATCTCAGGATTGCACAGTAAGCCGCTTAAAACGTGTACGTT
ATGGTGACATTTATCTTGATAAGAAACTGCCACGTGGCGGTTGGATGGAGCTTGAGCTGAAAGACGTGAACTACCTACGC
GAGTTGGTAGAGCTTCGTCCAGAGAAAGAGACCATGATTGAGTTGAATAACTCATCACGTAAGCGTGACCGTGCCCGTAG
TCAGAAGATTCGCCGCGCGGTAAAACGTCATGAAGAGCGCGTGAGTACGCCAAAAGGTCGCAGCAACAATGCGGCACGAC
GCAAGCCAAGTAAGGCCGATGGTCAAACAAACAAAACCAATAGCCGCAACCAAGCGGGTAGCCGCTCTAAGCCACAGCGC
TAA

Upstream 100 bases:

>100_bases
TATAATCGGCGCCTCGATTCCGAGCATCGGGTAGACATTGTAAACGGGTCTAGCTTGGTAAGACTAAGACGTCTGAGAAG
ACGACAATTTAGGTAGATAA

Downstream 100 bases:

>100_bases
GAGAACGAGCAGAAATGAAAAGGCTTGCCATTGGCAAGCCTTTTTTGCTTCTTAATCTCGTTTCCAGAGAATGTGACAGA
ACTTTTGGTCTGGATCGCGG

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVE
LDDGLARFEDVVYTGGEGMNHTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKADGQTNKTNSRNQAGSRSKPQR

Sequences:

>Translated_320_residues
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVE
LDDGLARFEDVVYTGGEGMNHTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKADGQTNKTNSRNQAGSRSKPQR
>Mature_320_residues
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVE
LDDGLARFEDVVYTGGEGMNHTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKADGQTNKTNSRNQAGSRSKPQR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=295, Percent_Identity=64.4067796610169, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=28.6307053941909, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=29.5652173913043, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_VIBVU (Q8D8C0)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761857.1
- ProteinModelPortal:   Q8D8C0
- SMR:   Q8D8C0
- GeneID:   1179891
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_3060
- HOGENOM:   HBG754478
- OMA:   ELCTRSD
- ProtClustDB:   CLSK874246
- BioCyc:   VVUL216895:VV1_3060-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKA
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEC
QEEVICRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVELDDGLARFEDVVYTGGEGMN
CHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCC
HTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
EEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHHHH
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAD
HHHHHCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
GQTNKTNSRNQAGSRSKPQR
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKA
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEC
QEEVICRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVELDDGLARFEDVVYTGGEGMN
CHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCC
HTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
EEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHHHH
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAD
HHHHHCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
GQTNKTNSRNQAGSRSKPQR
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA