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The map label for this gene is mlc [H]

Identifier: 27366265

GI number: 27366265

Start: 3058447

End: 3059664

Strand: Reverse

Name: mlc [H]

Synonym: VV1_2991

Alternate gene names: 27366265

Gene position: 3059664-3058447 (Counterclockwise)

Preceding gene: 27366267

Following gene: 27366264

Centisome position: 93.23

GC content: 46.8

Gene sequence:

>1218_bases
ATGTACATGGCTCAACCGGGCCATATTGATCATATCAAACAGGTCAATGCTGGTCGTGTATACAAACTAATTGACCTTAA
GGGTCCAATCTCTCGAATCGATTTGTCAAAAGAAAGCGAGCTGGCACCGGCGAGTATTACGAAAATTACCCGTGAGTTGA
TAGAAGCACATCTTGTGCACGAAACTACCGTGCAAGAAGCGATTAGCCGAGGAAGACCAGCGGTCGGCCTTCAAGTGAAT
AATGCTGGATGGCAATTTCTTTCGATGCGACTTGGACGAGGTTATCTCACCATTGCACTCCATGAATTGGGTGGTGAAGT
CCTGATCGATACAAAAATCGATATCCACGAAAGGGATCAAGATGACGTTCTTGCTCGACTCCTACACGAAATCGAAGAAT
TTTTCCAAACCTATGCCGATCAACTGGATCGTGTAACCAGTATCGCGATTACGTTGCCTGGCTTAGTCAATTCAGAACAA
GGCATTGTGCTGCAAATGCCGCATTACAATGTTGAAAACCTCGCTCTCGGTCCAGAGATTTACAAAGCGACTGGGCTACC
GGTTTTCATCGCCAATGACACACGAGCTTGGGCTTTGGCGGAAAAGCTATTTGGTCACTCTCAAGGTAACGAAAACTCGG
TTCTTATTTCGATCCACCATGGTTTAGGTGCGGGTATCATACTTGATGGACGGGTATTGCAGGGCCGTCACGGGAACATT
GGCGAATTAGGCCATATCCAGATCGATCCACAAGGCAAACTTTGCCACTGTGGCAACAGAGGGTGCCTTGAAACGGTCGC
AAGTTCTAAAGCCATTCGTGAAGAGGTAATGGAACGCATTGCTAATGGAGAGTCGTCCATTCTCGAAGAGCTGGAAGATG
TCACGATTGAAAGCATTTGTGAGGCCGCAGCGAATGGAGATGCATTAGCTGTGGATGTCATTGAGAAACTCGGCCGTTAT
CTTGGCTCGGCGATTGCGATTGTCATCAACTTATTTAACCCAGAAAAAGTGCTGATCGGCGGTGTAATCAACCAAGCGAA
GGAAGTTCTCTATCCGGCTATTCGACGCTGCATTGAAGAGCAAAGCTTGCCTGTTTATCACCAAGATCTTGAACTGGTGG
AATCGCGTTTCTATAAACAGGCAACCATGCCAGGGGCTGCATTGATTAAGCAGGCTCTTTACGATGGCTTGCTATTAATG
AAAGTGGTCGAAGGTTAA

Upstream 100 bases:

>100_bases
ATGTTTATACGTGATGCTGGTCACGGGGATTTATCAATTCACTTCACAATTACTTCGCAAAGTAAAAAAATTGCTCCGTT
ATAGAATGTTGGAGTGCAAG

Downstream 100 bases:

>100_bases
ATCCCAACTCGCGGCAAACCTGGATCTTCTCCAGGTTTTGTTTTATTCGGCCTTGAAATCAGTGACCTGCCTATCTCAAT
TGCCCCTCATCAATTGATCT

Product: transcriptional repressor of MalT

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKESELAPASITKITRELIEAHLVHETTVQEAISRGRPAVGLQVN
NAGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHERDQDDVLARLLHEIEEFFQTYADQLDRVTSIAITLPGLVNSEQ
GIVLQMPHYNVENLALGPEIYKATGLPVFIANDTRAWALAEKLFGHSQGNENSVLISIHHGLGAGIILDGRVLQGRHGNI
GELGHIQIDPQGKLCHCGNRGCLETVASSKAIREEVMERIANGESSILEELEDVTIESICEAAANGDALAVDVIEKLGRY
LGSAIAIVINLFNPEKVLIGGVINQAKEVLYPAIRRCIEEQSLPVYHQDLELVESRFYKQATMPGAALIKQALYDGLLLM
KVVEG

Sequences:

>Translated_405_residues
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKESELAPASITKITRELIEAHLVHETTVQEAISRGRPAVGLQVN
NAGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHERDQDDVLARLLHEIEEFFQTYADQLDRVTSIAITLPGLVNSEQ
GIVLQMPHYNVENLALGPEIYKATGLPVFIANDTRAWALAEKLFGHSQGNENSVLISIHHGLGAGIILDGRVLQGRHGNI
GELGHIQIDPQGKLCHCGNRGCLETVASSKAIREEVMERIANGESSILEELEDVTIESICEAAANGDALAVDVIEKLGRY
LGSAIAIVINLFNPEKVLIGGVINQAKEVLYPAIRRCIEEQSLPVYHQDLELVESRFYKQATMPGAALIKQALYDGLLLM
KVVEG
>Mature_405_residues
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKESELAPASITKITRELIEAHLVHETTVQEAISRGRPAVGLQVN
NAGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHERDQDDVLARLLHEIEEFFQTYADQLDRVTSIAITLPGLVNSEQ
GIVLQMPHYNVENLALGPEIYKATGLPVFIANDTRAWALAEKLFGHSQGNENSVLISIHHGLGAGIILDGRVLQGRHGNI
GELGHIQIDPQGKLCHCGNRGCLETVASSKAIREEVMERIANGESSILEELEDVTIESICEAAANGDALAVDVIEKLGRY
LGSAIAIVINLFNPEKVLIGGVINQAKEVLYPAIRRCIEEQSLPVYHQDLELVESRFYKQATMPGAALIKQALYDGLLLM
KVVEG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=206, Percent_Identity=31.0679611650485, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4885285, Length=206, Percent_Identity=31.0679611650485, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI298566315, Length=206, Percent_Identity=31.0679611650485, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI190014632, Length=207, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787878, Length=402, Percent_Identity=46.5174129353234, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786891, Length=401, Percent_Identity=43.8902743142145, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI87082127, Length=217, Percent_Identity=24.8847926267281, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790522, Length=205, Percent_Identity=26.3414634146341, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082230, Length=227, Percent_Identity=27.7533039647577, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 44470; Mature: 44470

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKESELAPASITKITRELIEAHLVH
CCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ETTVQEAISRGRPAVGLQVNNAGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHERDQ
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCH
DDVLARLLHEIEEFFQTYADQLDRVTSIAITLPGLVNSEQGIVLQMPHYNVENLALGPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEECCCCCCCCEECCCHH
YKATGLPVFIANDTRAWALAEKLFGHSQGNENSVLISIHHGLGAGIILDGRVLQGRHGNI
HHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCEEECCCCCCC
GELGHIQIDPQGKLCHCGNRGCLETVASSKAIREEVMERIANGESSILEELEDVTIESIC
CCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EAAANGDALAVDVIEKLGRYLGSAIAIVINLFNPEKVLIGGVINQAKEVLYPAIRRCIEE
HHHCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
QSLPVYHQDLELVESRFYKQATMPGAALIKQALYDGLLLMKVVEG
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKESELAPASITKITRELIEAHLVH
CCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ETTVQEAISRGRPAVGLQVNNAGWQFLSMRLGRGYLTIALHELGGEVLIDTKIDIHERDQ
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCH
DDVLARLLHEIEEFFQTYADQLDRVTSIAITLPGLVNSEQGIVLQMPHYNVENLALGPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEECCCCCCCCEECCCHH
YKATGLPVFIANDTRAWALAEKLFGHSQGNENSVLISIHHGLGAGIILDGRVLQGRHGNI
HHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCEEECCCCCCC
GELGHIQIDPQGKLCHCGNRGCLETVASSKAIREEVMERIANGESSILEELEDVTIESIC
CCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EAAANGDALAVDVIEKLGRYLGSAIAIVINLFNPEKVLIGGVINQAKEVLYPAIRRCIEE
HHHCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
QSLPVYHQDLELVESRFYKQATMPGAALIKQALYDGLLLMKVVEG
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]