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The map label for this gene is rlmF

Identifier: 27366061

GI number: 27366061

Start: 2838138

End: 2839232

Strand: Reverse

Name: rlmF

Synonym: VV1_2774

Alternate gene names: 27366061

Gene position: 2839232-2838138 (Counterclockwise)

Preceding gene: 326424072

Following gene: 326424071

Centisome position: 86.51

GC content: 47.76

Gene sequence:

>1095_bases
ATGACGAACAAACGAAAATCCGCCAAGCCATTAGAGCCTGCGAAACGAGCCCCAAAACCGCGCACGAAAAAGAGTCGTGA
TCTCAGTGCGTCTGAATCGAATTGTGATTTTGTCAAAGTGACGCGTGCTGGGCTGCATTCGCGCAACAAACATCAAGGGC
GTTACGATTTTGCTAAGTTAACGCAGGCGCTGCCATCGCTTGCTCCTTTTGTGATCAAAAATCCTAAAGGTGAGGCGAGT
ATTTCTTTTTCAGATTCAACGGCAGTAAAAATGCTCAATAAAGCGCTGCTCAGTGCTTACTACCAAGTAGCAAATTGGGA
CATCCCAGCAGGCTATTTGTGCCCGCCAATCCCCGGGCGTGCGGATTATATCCATCGTCTAGCGGAGCTCTTGGAAGGAG
AGGTGAAAGGTAAATTTCCTCATGAGAAAGTTCAGGCGTTGGACATCGGTGTCGGTGCAAACGCCATCTATCCCATTATT
GCGATTTGTGACTATCGTTGGCGCTACACGGGTAGTGACGTTGACCCTAAATCGATTGAGTCAGCGCAGCGCATTGCTGA
CTCTAACCCTGTGCTGCAGGGGCAGCTTGAGCTCAAATTGCAAGACCAGTCACAGCATATTTTCCAAGGCATTATTGGCC
CCACAGACTACTTTCATGTGACGACATGCAACCCGCCATTTCACGCTTCGGCACAGGAAGCGGCGTTTGGCACGCAACGA
AAATTAGACAACCTCGCCGCCAACCGTTTGAAAAAAGGTGTAACAGCAAAAGCTGGTTCACAAAAAATCTCGAAAAACAA
ACCTATACTTAACTTCGGAGGTCAAAATTCTGAGTTGTGGTGTCAAGGTGGAGAATCCTCCTTCTTGAAGCGCATGGCGA
ACGAAAGTGAGCGTTTTGCCCATCAGGTTTTGTGGTTCAGTACGCTCGTTTCGAAGAAAGACAACGTAAGACCTCTACGT
AAACAGTTGGAAAAATTAGGTGTACGCAGTATTCGTGTCGTCGAAATGAGCCAAGGACAAAAGGTCAGTCGCTTTGTTGC
TTGGTCATTTATGGACAAACAACAAAGAGGAGAGTGGATCAAACTGCGAGGGTGA

Upstream 100 bases:

>100_bases
CTCTTTGCTGCTACATGGAGCCGGATTATCACCAACTGGAGCTGGATGCTCGTCTGTTTATGATTTACCTCGATTTAAAA
GCTCAGGAAGCAAAAAAATA

Downstream 100 bases:

>100_bases
TGGAGACAATCATCCCGAGTTAAAAACGAATAGGTTGCGTAATAGGAGGGCATGCGATGGATTTTGAAATTTATCTACCC
TGTCAGCCAGATTGGCTGTG

Product: 23S rRNA mA1618 methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTNKRKSAKPLEPAKRAPKPRTKKSRDLSASESNCDFVKVTRAGLHSRNKHQGRYDFAKLTQALPSLAPFVIKNPKGEAS
ISFSDSTAVKMLNKALLSAYYQVANWDIPAGYLCPPIPGRADYIHRLAELLEGEVKGKFPHEKVQALDIGVGANAIYPII
AICDYRWRYTGSDVDPKSIESAQRIADSNPVLQGQLELKLQDQSQHIFQGIIGPTDYFHVTTCNPPFHASAQEAAFGTQR
KLDNLAANRLKKGVTAKAGSQKISKNKPILNFGGQNSELWCQGGESSFLKRMANESERFAHQVLWFSTLVSKKDNVRPLR
KQLEKLGVRSIRVVEMSQGQKVSRFVAWSFMDKQQRGEWIKLRG

Sequences:

>Translated_364_residues
MTNKRKSAKPLEPAKRAPKPRTKKSRDLSASESNCDFVKVTRAGLHSRNKHQGRYDFAKLTQALPSLAPFVIKNPKGEAS
ISFSDSTAVKMLNKALLSAYYQVANWDIPAGYLCPPIPGRADYIHRLAELLEGEVKGKFPHEKVQALDIGVGANAIYPII
AICDYRWRYTGSDVDPKSIESAQRIADSNPVLQGQLELKLQDQSQHIFQGIIGPTDYFHVTTCNPPFHASAQEAAFGTQR
KLDNLAANRLKKGVTAKAGSQKISKNKPILNFGGQNSELWCQGGESSFLKRMANESERFAHQVLWFSTLVSKKDNVRPLR
KQLEKLGVRSIRVVEMSQGQKVSRFVAWSFMDKQQRGEWIKLRG
>Mature_363_residues
TNKRKSAKPLEPAKRAPKPRTKKSRDLSASESNCDFVKVTRAGLHSRNKHQGRYDFAKLTQALPSLAPFVIKNPKGEASI
SFSDSTAVKMLNKALLSAYYQVANWDIPAGYLCPPIPGRADYIHRLAELLEGEVKGKFPHEKVQALDIGVGANAIYPIIA
ICDYRWRYTGSDVDPKSIESAQRIADSNPVLQGQLELKLQDQSQHIFQGIIGPTDYFHVTTCNPPFHASAQEAAFGTQRK
LDNLAANRLKKGVTAKAGSQKISKNKPILNFGGQNSELWCQGGESSFLKRMANESERFAHQVLWFSTLVSKKDNVRPLRK
QLEKLGVRSIRVVEMSQGQKVSRFVAWSFMDKQQRGEWIKLRG

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=310, Percent_Identity=27.4193548387097, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI87081786, Length=319, Percent_Identity=48.2758620689655, Blast_Score=315, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32564931, Length=310, Percent_Identity=22.258064516129, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32564933, Length=310, Percent_Identity=20.3225806451613, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281363415, Length=317, Percent_Identity=25.8675078864353, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922302, Length=317, Percent_Identity=25.8675078864353, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_VIBVU (Q8D935)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761589.1
- ProteinModelPortal:   Q8D935
- SMR:   Q8D935
- GeneID:   1179619
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2774
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   VVUL216895:VV1_2774-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 40722; Mature: 40591

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKRKSAKPLEPAKRAPKPRTKKSRDLSASESNCDFVKVTRAGLHSRNKHQGRYDFAKL
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCHHHHHH
TQALPSLAPFVIKNPKGEASISFSDSTAVKMLNKALLSAYYQVANWDIPAGYLCPPIPGR
HHHHHHHCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
ADYIHRLAELLEGEVKGKFPHEKVQALDIGVGANAIYPIIAICDYRWRYTGSDVDPKSIE
HHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHCCCEEECCCCCCHHHHH
SAQRIADSNPVLQGQLELKLQDQSQHIFQGIIGPTDYFHVTTCNPPFHASAQEAAFGTQR
HHHHHHCCCCEEEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
KLDNLAANRLKKGVTAKAGSQKISKNKPILNFGGQNSELWCQGGESSFLKRMANESERFA
HHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHH
HQVLWFSTLVSKKDNVRPLRKQLEKLGVRSIRVVEMSQGQKVSRFVAWSFMDKQQRGEWI
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEE
KLRG
EECC
>Mature Secondary Structure 
TNKRKSAKPLEPAKRAPKPRTKKSRDLSASESNCDFVKVTRAGLHSRNKHQGRYDFAKL
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCHHHHHH
TQALPSLAPFVIKNPKGEASISFSDSTAVKMLNKALLSAYYQVANWDIPAGYLCPPIPGR
HHHHHHHCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
ADYIHRLAELLEGEVKGKFPHEKVQALDIGVGANAIYPIIAICDYRWRYTGSDVDPKSIE
HHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHCCCEEECCCCCCHHHHH
SAQRIADSNPVLQGQLELKLQDQSQHIFQGIIGPTDYFHVTTCNPPFHASAQEAAFGTQR
HHHHHHCCCCEEEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
KLDNLAANRLKKGVTAKAGSQKISKNKPILNFGGQNSELWCQGGESSFLKRMANESERFA
HHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHH
HQVLWFSTLVSKKDNVRPLRKQLEKLGVRSIRVVEMSQGQKVSRFVAWSFMDKQQRGEWI
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEE
KLRG
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA