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The map label for this gene is prpC [H]

Identifier: 27366021

GI number: 27366021

Start: 2792739

End: 2793881

Strand: Reverse

Name: prpC [H]

Synonym: VV1_2731

Alternate gene names: 27366021

Gene position: 2793881-2792739 (Counterclockwise)

Preceding gene: 27366022

Following gene: 27366020

Centisome position: 85.13

GC content: 51.44

Gene sequence:

>1143_bases
ATGCCTAGTTCTTTAACGAAGAAAGGTGAGTTAGGTGGCGCAGGCCTGCGTGGTCAAAGCGCTGGAAGCACCGCTTTATG
TACTGTCGGTAAAACGGGGACAGGGTTAACCTATCGTGGTTATGACATCACTGACTTGGCCAACCATGCGCAATTTGAAG
AAGTGGCGTACTTACTGTTACGCGGTCACTTACCAACGCAGCAAGAGTTGGATGCTTACAAAACTCGCCTGATTGGCCTG
CGTGGCTTACCGGTGGAGTTGAAGCAAGCGCTGGAGCTGATCCCGGCGTCTGCGCATCCGATGGATGTGATGCGCACAGG
CTGCTCTGTGCTGGGGAACTTAGAACAAGAAACCGATTTTTCGCAGCAGCTTGACGCGACAGAGCGCATGTTGGCGCTGT
TCCCTGCGATCATTTGTTACTGGTATCGCTTTAGCCACGATGGCGTGCGCATCGATACGCAAGACCAATCGCAAGATTGC
ATCGGTGGTTACTTCCTCAAAATGCTCACGGGCAAAGAGCCCAGTGAACTGCATAAGCAGGTGATGCATTGTTCGCTGAT
TTTGTATGCAGAGCACGAGTTTAATGCCTCTACCTTTGCCGCTCGTGTGTGCGCCTCAACGCTGTCTGATATTCATTCTT
GTGTAACCGCGGCGATTGGCACTTTGCGTGGTCCTTTGCATGGTGGTGCTAATGAGGCCGCGATGGAGATGATCGAAAAC
TGGCAAACACCAGAAGAGGCGGAAAGCAACATCCTGCGCATGTTGGCCAACAAAGAGAAAATCATGGGCTTTGGTCATGC
GATTTATCGCGAAAGCGACCCACGTAATGCTCTGATCAAACGTTGGTCAAAAGCCTTGTCAGAAGCGGTGGGCGATACCC
ATCTTTACGCCGTCTCAGAACGTGTGGAAGCGGTGATGAAACGCGAGAAAGATCTGTTTTGTAACGCCGATTTCTTCCAT
GCCTCGGCCTATCACTTCATGGACATTCCCACCAAGCTGTTTACGCCGATTTTCGTCATGAGTCGCTTAACAGGCTGGAC
GGCGCATGTGTTTGAACAGCGCGCTAATAACCGAATCATTCGTCCAAGTGCTGACTACATTGGCCCAGATCACCAAGATT
GGGTGCCGATTGAACAGCGCTAG

Upstream 100 bases:

>100_bases
AAGAAGAATACCGTCGGAATTAACTTAATCCAATAACGTGAAACCGTCTGATGATAAACGCTCCACTAGAGAGCAACGTC
AGACAGAAAAGGAGTTCAAA

Downstream 100 bases:

>100_bases
CGCCTAGAGATTGACCTCTCCTCGTCGCGAGGGGAGGTTTTCCGCCCTTTTGCATGGGAAGCAAGTCCATGAGCAATAAA
AACAATACCTTATATCGCAA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAYLLLRGHLPTQQELDAYKTRLIGL
RGLPVELKQALELIPASAHPMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIICYWYRFSHDGVRIDTQDQSQDC
IGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAIGTLRGPLHGGANEAAMEMIEN
WQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVSERVEAVMKREKDLFCNADFFH
ASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQR

Sequences:

>Translated_380_residues
MPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAYLLLRGHLPTQQELDAYKTRLIGL
RGLPVELKQALELIPASAHPMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIICYWYRFSHDGVRIDTQDQSQDC
IGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAIGTLRGPLHGGANEAAMEMIEN
WQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVSERVEAVMKREKDLFCNADFFH
ASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQR
>Mature_379_residues
PSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAYLLLRGHLPTQQELDAYKTRLIGLR
GLPVELKQALELIPASAHPMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIICYWYRFSHDGVRIDTQDQSQDCI
GGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAIGTLRGPLHGGANEAAMEMIENW
QTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVSERVEAVMKREKDLFCNADFFHA
SAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=51.3586956521739, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786939, Length=362, Percent_Identity=31.4917127071823, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=357, Percent_Identity=22.6890756302521, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=390, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324328, Length=245, Percent_Identity=26.1224489795918, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640126, Length=393, Percent_Identity=23.4096692111959, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640124, Length=393, Percent_Identity=23.4096692111959, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356863, Length=362, Percent_Identity=23.2044198895028, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42416; Mature: 42285

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAYLLL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
RGHLPTQQELDAYKTRLIGLRGLPVELKQALELIPASAHPMDVMRTGCSVLGNLEQETDF
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCH
SQQLDATERMLALFPAIICYWYRFSHDGVRIDTQDQSQDCIGGYFLKMLTGKEPSELHKQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
VMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAIGTLRGPLHGGANEAAMEMIEN
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
WQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
RVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRII
HHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEE
RPSADYIGPDHQDWVPIEQR
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAYLLL
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
RGHLPTQQELDAYKTRLIGLRGLPVELKQALELIPASAHPMDVMRTGCSVLGNLEQETDF
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCH
SQQLDATERMLALFPAIICYWYRFSHDGVRIDTQDQSQDCIGGYFLKMLTGKEPSELHKQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
VMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAIGTLRGPLHGGANEAAMEMIEN
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
WQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
RVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRII
HHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEE
RPSADYIGPDHQDWVPIEQR
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]