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The map label for this gene is prpF [H]

Identifier: 27366019

GI number: 27366019

Start: 2788841

End: 2790013

Strand: Reverse

Name: prpF [H]

Synonym: VV1_2729

Alternate gene names: 27366019

Gene position: 2790013-2788841 (Counterclockwise)

Preceding gene: 27366020

Following gene: 27366018

Centisome position: 85.01

GC content: 53.37

Gene sequence:

>1173_bases
ATGAGCCAAATGAAAGTGCCTGCCACGTATATGCGAGGCGGCACCAGCAAGGGGGTGTTTTTCAACCTTGCGGATTTACC
GCCAGAGGCACAAATTGCGGGGGAAAAACGCGACCAACTGCTGCTGCGCGTGATCGGCAGCCCAGATCCTTACGGCAAAC
AGATTGACGGCATGGGCGGGGCGACATCCAGCACCAGCAAAACGGTGATTGTGAGCAAAAGCAGCCGCGCCGATCACGAT
GTTGATTACCTGTTTGGTCAAGTCTCTATCGACAAACCGTTTGTCGATTGGAGCGGTAACTGTGGCAACTTGTCTGCTGC
GGTTGGCCCATTTGCCATCCATGCTGGCTTGATTGACGAAAAACGCATTCCTCAAAATGGCGTGGTGACGGTGAAAGTGT
GGCAAGCCAACATTGGCAAAACCATTGAAGTGCACGTGCCCATTCAACATGGTTTGGTGCAGGAAACGGGCGAGTTTTCT
CTTGATGGCGTGACATTTCCTGCTGCAGAGATCCAAGTGGATTTCGTTGACCCAGCCGATGGCGAGGGGAGCATGTTTCC
CACTGGCAATTTGATGGATGATCTTGTCGTACCGGGTATTGGCACCTTTAACGCCACGCTCATTAATGCGGGCATTCCCA
CCATTTTCCTTGATGCTGAGGCGTTGGGGTATTTGGGGACGGAACTGCAAGAGCAGATCAATAATGATGAGCAAGCGTTA
GCGAAGTTTGAAACCATTCGTGCTTATGGCGCGCTAAAAATGGGTTTGATTACTTCGATTGAAGAAGCAGAAGGTCGCCA
GCACACCCCAAAAGTGGCGTTTGTCGCGAAGCCGAAAAGTTACCAAGCCTCAAGTGGCAAAGTGGTGGAGGCAGGAGAGG
TTGATCTGCTGGTTCGCGCCCTTTCAATGGGCAAGCTGCATCACGCCATGATGGGGACCGCAGCGGTCGCGATTGGCGCA
GCCGCTTGCGTGCCGGGCACTTTAGTGAACTTGGCTGCCGGTGGTGGCGAGAAACAAGCCGTCACCTTTGGCCATCCGTC
TGGGACGCTCAAAGTCGGCGCTCAAGCAAAAGCCACTGAACAAGGTTGGGTGGTAGAAAAAGCCATTATGAGCCGCAGCG
CTCGCATACTGATGGAGGGCTTTGTGCGTGTTCCCTCCGATCTCTTTGAATAA

Upstream 100 bases:

>100_bases
TGCAGCGATTCGCGCAAGATTTTCTCTCCGAGCAAGGAGTGTAACGATGAAACCTTCTACACAATCCTCTACGCGTAGCT
CTGCACGGTGCGATACGCGG

Downstream 100 bases:

>100_bases
ATCTTAGCTTACTTGTTCAAAGAGCAAGGTGAAAAGGACCGTTAAGACATACTGGAGGAAGCACTATGTCTGCTTATCAG
AAAGAATATCAATGGGCGAA

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSSRADHD
VDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANIGKTIEVHVPIQHGLVQETGEFS
LDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLDAEALGYLGTELQEQINNDEQAL
AKFETIRAYGALKMGLITSIEEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLVRALSMGKLHHAMMGTAAVAIGA
AACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILMEGFVRVPSDLFE

Sequences:

>Translated_390_residues
MSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSSRADHD
VDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANIGKTIEVHVPIQHGLVQETGEFS
LDGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLDAEALGYLGTELQEQINNDEQAL
AKFETIRAYGALKMGLITSIEEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLVRALSMGKLHHAMMGTAAVAIGA
AACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILMEGFVRVPSDLFE
>Mature_389_residues
SQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSSRADHDV
DYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDEKRIPQNGVVTVKVWQANIGKTIEVHVPIQHGLVQETGEFSL
DGVTFPAAEIQVDFVDPADGEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLDAEALGYLGTELQEQINNDEQALA
KFETIRAYGALKMGLITSIEEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLVRALSMGKLHHAMMGTAAVAIGAA
ACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATEQGWVVEKAIMSRSARILMEGFVRVPSDLFE

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=32.7272727272727, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41123; Mature: 40992

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGMGG
CCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHCCCCHHEEEEEEECCCCCCCCCCCCCCC
ATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDE
CCCCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCC
KRIPQNGVVTVKVWQANIGKTIEVHVPIQHGLVQETGEFSLDGVTFPAAEIQVDFVDPAD
CCCCCCCEEEEEEEECCCCCEEEEEECHHHCCHHCCCCEEECCEECCCEEEEEEEECCCC
GEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLDAEALGYLGTELQEQINNDEQAL
CCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHCCHHHHH
AKFETIRAYGALKMGLITSIEEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLVRA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHH
LSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEECCCCCCCC
QGWVVEKAIMSRSARILMEGFVRVPSDLFE
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
SQMKVPATYMRGGTSKGVFFNLADLPPEAQIAGEKRDQLLLRVIGSPDPYGKQIDGMGG
CCCCCCHHHHCCCCCCCEEEEECCCCCCCHHCCCCHHEEEEEEECCCCCCCCCCCCCCC
ATSSTSKTVIVSKSSRADHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIHAGLIDE
CCCCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCC
KRIPQNGVVTVKVWQANIGKTIEVHVPIQHGLVQETGEFSLDGVTFPAAEIQVDFVDPAD
CCCCCCCEEEEEEEECCCCCEEEEEECHHHCCHHCCCCEEECCEECCCEEEEEEEECCCC
GEGSMFPTGNLMDDLVVPGIGTFNATLINAGIPTIFLDAEALGYLGTELQEQINNDEQAL
CCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHCCHHHHH
AKFETIRAYGALKMGLITSIEEAEGRQHTPKVAFVAKPKSYQASSGKVVEAGEVDLLVRA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHH
LSMGKLHHAMMGTAAVAIGAAACVPGTLVNLAAGGGEKQAVTFGHPSGTLKVGAQAKATE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEECCCCCCCC
QGWVVEKAIMSRSARILMEGFVRVPSDLFE
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA