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The map label for this gene is hlyB [H]

Identifier: 27365579

GI number: 27365579

Start: 2270119

End: 2271744

Strand: Reverse

Name: hlyB [H]

Synonym: VV1_2255

Alternate gene names: 27365579

Gene position: 2271744-2270119 (Counterclockwise)

Preceding gene: 326423975

Following gene: 27365578

Centisome position: 69.22

GC content: 49.69

Gene sequence:

>1626_bases
ATGCGCTCAACCATTACGTTCAAATTACTTGTTGCCCTTATCACCGTCTTTACCTTTGTCCTAGCGGTATCGACAGCTTA
TCAATATTATCAACAAAAACAGCTTATTAACTCTGTTTTAAGTGAACAACTGCACGACAAGGCCAGTAACTATTTCGACA
GCCTAAATATGATGATGCTCACGGGCACTATGGCCCAGAAAGAGACATTGCGACAAAAAGCCCTCGCCCAAGATGGTATT
GAGCAAGTCCGTGTATTACGCAGTGAGTTGGTGAGTAAGTTGTATGGTCCAGGCCAAGCGAATCAAGCCCCACAAGATGA
CATCGATCAGAGAGCGTTAAATGGTGAGTTGGTGATCGAACCTGTGGAAGCCGATTGGGGCAAAGGTTTGGTCGTCGCTC
TGCCAATGAAATCCAGCGAGAATTACCGAGGCACCAATTGTGTGGCCTGTCATATGGCTGCAGAAGGCGAAGTCTTGGGT
GCAATTCGTTTGGAATACAACTTAAGCCATGTCAATTCGATGATTAATAAGCAAGCGATGTTTGCCATTGCGATTATGTC
TTCGATTGCTTTTGTGGGCTTTTTGATCACCATGACGCTGATCCGCAAGATCATTGTACGCCCTATTCAAGCCACATCGC
ACTTTATGACCAAAGTCAGTAAAAGCAAGGATCTCGCCCAACGCGTTACTATCGCTCAAAATGACGAGGTAGGTGTGCTG
GCCACATCAATTAACTCCTTTATGGACACGGTCACAGAGAGCCTTAATCAAGTACAATCGACCTCTCACTCGCTCGCTAA
CTCAGCGACCCGCCTAACGCATGTGGCCCAACGCACCGACTCTGCGGCAAACAACCAACAACAAGAAACCAGCGATGTAC
AAAACGCCATCGAAGCGATGCAACATCAGCAGCTCGAAGTCGAACAAGCCACCGTCAATGCAACCGAGTTGGTGAATCAC
ACCGTGGAAGTGGCAACCACCAGCTCCAACCAAGCGCACAATGCCAGTGAAGAGATCAAAAATCTCGTCGGCGATATTGA
TAAAGTACGAGACCGGATTTCTCAGTTGAATGCGCAAACCGACGAGGTTTCGTCAATTTTAAGTGTGATTCACGCCATTG
CCGATCAAACGAACCTGCTGGCATTGAATGCTGCCATTGAAGCCGCTCGCGCCGGAGAACAAGGCCGAGGCTTTGCCGTG
GTTGCCGATGAAGTACGCAGCCTAGCCTCCCGCACTGCCGTCGCCACAGGCGATATCGGCTCGATCATCGCTCAATTCCA
ACAAGGCAGTTCCGACTCTCTGCGCGCGGCGGCGCAAGTCTCCGAGCATGCCCACCAGCGCTCTCTTGAAGTAGAAGCCT
TGTCTGACACCATGCGCAAAGTGGTTGAGGAGATGCATCAAGTCCTCTCGCACACCAAGCACATTCAATCGCAAACGCAA
GCAACCAGCCAATCCAGCCTAGCGATTCAAGCCAAGATTGGCACCATCACCCAACACGCCGATGACACCTCGCATTCCGC
GAGCCATACGCGCGATATCTCTGTCGAGTTAGAACAGCTTTCAGAGCATCTAGAGCAATTAATCAACCAATTCACCCTCA
ACAACCGTTCGTTTCAGTCGAAATGA

Upstream 100 bases:

>100_bases
TATTTTGTCTATGGTCACACTGCAGAAATCTCATGATCGCGACACTTTAAACGTCAGCTTAAAATATTGAGGTCTATTCC
AAAACTCAATTATAAAAACT

Downstream 100 bases:

>100_bases
AAGAATTTCTTGGCTGGAATGAACATTAAAGGTTGAAGCTGGCGAATTGACAGGTAATATGTTCCGATGAATCTGTAAAA
TTCTATCCAACCCAATGTTT

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MRSTITFKLLVALITVFTFVLAVSTAYQYYQQKQLINSVLSEQLHDKASNYFDSLNMMMLTGTMAQKETLRQKALAQDGI
EQVRVLRSELVSKLYGPGQANQAPQDDIDQRALNGELVIEPVEADWGKGLVVALPMKSSENYRGTNCVACHMAAEGEVLG
AIRLEYNLSHVNSMINKQAMFAIAIMSSIAFVGFLITMTLIRKIIVRPIQATSHFMTKVSKSKDLAQRVTIAQNDEVGVL
ATSINSFMDTVTESLNQVQSTSHSLANSATRLTHVAQRTDSAANNQQQETSDVQNAIEAMQHQQLEVEQATVNATELVNH
TVEVATTSSNQAHNASEEIKNLVGDIDKVRDRISQLNAQTDEVSSILSVIHAIADQTNLLALNAAIEAARAGEQGRGFAV
VADEVRSLASRTAVATGDIGSIIAQFQQGSSDSLRAAAQVSEHAHQRSLEVEALSDTMRKVVEEMHQVLSHTKHIQSQTQ
ATSQSSLAIQAKIGTITQHADDTSHSASHTRDISVELEQLSEHLEQLINQFTLNNRSFQSK

Sequences:

>Translated_541_residues
MRSTITFKLLVALITVFTFVLAVSTAYQYYQQKQLINSVLSEQLHDKASNYFDSLNMMMLTGTMAQKETLRQKALAQDGI
EQVRVLRSELVSKLYGPGQANQAPQDDIDQRALNGELVIEPVEADWGKGLVVALPMKSSENYRGTNCVACHMAAEGEVLG
AIRLEYNLSHVNSMINKQAMFAIAIMSSIAFVGFLITMTLIRKIIVRPIQATSHFMTKVSKSKDLAQRVTIAQNDEVGVL
ATSINSFMDTVTESLNQVQSTSHSLANSATRLTHVAQRTDSAANNQQQETSDVQNAIEAMQHQQLEVEQATVNATELVNH
TVEVATTSSNQAHNASEEIKNLVGDIDKVRDRISQLNAQTDEVSSILSVIHAIADQTNLLALNAAIEAARAGEQGRGFAV
VADEVRSLASRTAVATGDIGSIIAQFQQGSSDSLRAAAQVSEHAHQRSLEVEALSDTMRKVVEEMHQVLSHTKHIQSQTQ
ATSQSSLAIQAKIGTITQHADDTSHSASHTRDISVELEQLSEHLEQLINQFTLNNRSFQSK
>Mature_541_residues
MRSTITFKLLVALITVFTFVLAVSTAYQYYQQKQLINSVLSEQLHDKASNYFDSLNMMMLTGTMAQKETLRQKALAQDGI
EQVRVLRSELVSKLYGPGQANQAPQDDIDQRALNGELVIEPVEADWGKGLVVALPMKSSENYRGTNCVACHMAAEGEVLG
AIRLEYNLSHVNSMINKQAMFAIAIMSSIAFVGFLITMTLIRKIIVRPIQATSHFMTKVSKSKDLAQRVTIAQNDEVGVL
ATSINSFMDTVTESLNQVQSTSHSLANSATRLTHVAQRTDSAANNQQQETSDVQNAIEAMQHQQLEVEQATVNATELVNH
TVEVATTSSNQAHNASEEIKNLVGDIDKVRDRISQLNAQTDEVSSILSVIHAIADQTNLLALNAAIEAARAGEQGRGFAV
VADEVRSLASRTAVATGDIGSIIAQFQQGSSDSLRAAAQVSEHAHQRSLEVEALSDTMRKVVEEMHQVLSHTKHIQSQTQ
ATSQSSLAIQAKIGTITQHADDTSHSASHTRDISVELEQLSEHLEQLINQFTLNNRSFQSK

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1789453, Length=293, Percent_Identity=32.4232081911263, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787690, Length=363, Percent_Identity=29.2011019283747, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788195, Length=303, Percent_Identity=29.7029702970297, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI2367378, Length=331, Percent_Identity=29.0030211480363, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788194, Length=357, Percent_Identity=28.2913165266106, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 59174; Mature: 59174

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTITFKLLVALITVFTFVLAVSTAYQYYQQKQLINSVLSEQLHDKASNYFDSLNMMML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGTMAQKETLRQKALAQDGIEQVRVLRSELVSKLYGPGQANQAPQDDIDQRALNGELVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEE
PVEADWGKGLVVALPMKSSENYRGTNCVACHMAAEGEVLGAIRLEYNLSHVNSMINKQAM
ECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEEEECHHHHHHHHHHHHH
FAIAIMSSIAFVGFLITMTLIRKIIVRPIQATSHFMTKVSKSKDLAQRVTIAQNDEVGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ATSINSFMDTVTESLNQVQSTSHSLANSATRLTHVAQRTDSAANNQQQETSDVQNAIEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QHQQLEVEQATVNATELVNHTVEVATTSSNQAHNASEEIKNLVGDIDKVRDRISQLNAQT
HHHHHHHHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
DEVSSILSVIHAIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLASRTAVATGDIG
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCHHH
SIIAQFQQGSSDSLRAAAQVSEHAHQRSLEVEALSDTMRKVVEEMHQVLSHTKHIQSQTQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATSQSSLAIQAKIGTITQHADDTSHSASHTRDISVELEQLSEHLEQLINQFTLNNRSFQS
HCCCCCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
K
C
>Mature Secondary Structure
MRSTITFKLLVALITVFTFVLAVSTAYQYYQQKQLINSVLSEQLHDKASNYFDSLNMMML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGTMAQKETLRQKALAQDGIEQVRVLRSELVSKLYGPGQANQAPQDDIDQRALNGELVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEE
PVEADWGKGLVVALPMKSSENYRGTNCVACHMAAEGEVLGAIRLEYNLSHVNSMINKQAM
ECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEEEECHHHHHHHHHHHHH
FAIAIMSSIAFVGFLITMTLIRKIIVRPIQATSHFMTKVSKSKDLAQRVTIAQNDEVGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ATSINSFMDTVTESLNQVQSTSHSLANSATRLTHVAQRTDSAANNQQQETSDVQNAIEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QHQQLEVEQATVNATELVNHTVEVATTSSNQAHNASEEIKNLVGDIDKVRDRISQLNAQT
HHHHHHHHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
DEVSSILSVIHAIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLASRTAVATGDIG
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCHHH
SIIAQFQQGSSDSLRAAAQVSEHAHQRSLEVEALSDTMRKVVEEMHQVLSHTKHIQSQTQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATSQSSLAIQAKIGTITQHADDTSHSASHTRDISVELEQLSEHLEQLINQFTLNNRSFQS
HCCCCCEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]