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The map label for this gene is rpfG [H]

Identifier: 27365573

GI number: 27365573

Start: 2264131

End: 2265585

Strand: Reverse

Name: rpfG [H]

Synonym: VV1_2249

Alternate gene names: 27365573

Gene position: 2265585-2264131 (Counterclockwise)

Preceding gene: 326423973

Following gene: 27365572

Centisome position: 69.03

GC content: 47.01

Gene sequence:

>1455_bases
ATGTCTCATTACAACGCGCCATTAACTTTCAAACTCTATGCGCTTGCCGCCATCGTTTTTGGTACTTATGGCGGTCGCGT
TTGTCCTATGCTGGCAACGCTGTCACCGCTAGAAGTGCTGGTGCACGTTTCTATTACTTTTCTTGCTGTCTTTGCTGTAC
GACACTTTTTCTTTTCTCAACATCGATGGGTCCGCGAGCAAAAACACGCGCAGCTCGATACCGTGTTGTTTTTTATCGGC
AGCTTACCTCTTGCCTACTACTACAACAGCCAATATGACTTCACCATTGATAGCAACTTAAAAGTGCTGTTTGGTATGAC
GTTGTTTGGCTTTTTTACTGGATTGATCCTGCAACTCAAAGCCAAATTACTTCGCTTTGAGCTTCTTGAGGAAAATGCCA
CCCGCCAAGATACACTGAGTGGCGAGCGACAATCGATTATCAAACAGCTCATTTTGATGATGACGCTGCTTATCATCGCG
TTGACCACCATGCTGACCATGGTGGCGGTGAAAGACATTTTTTGGCTGGAGCACAACCCAGAGAGATTACTCGATGGCAG
TGGCAAGATCAGCATCATTAAAGAGTTCATCTATTTAGGCGTAGTACTAGGTGGGTATGTCCTAGCCATTCTCCTCTTGT
GGTCGAAACTCATGAAAAAGATTTTGTTGCGACAAGAACATTCCCTACAAGAAGTGACCAATGGTGCAATAGGATGCCGT
CTGGCTGTTTTCGAGCATGATGAATTGGGTTCAATGGCGGCGCTCACCAACAAAATGTTGGATAGCCTGCAAGCATCGCA
AGATGAGGTAAAAACCACTCGAGATGTGGCCATCGTCAGTCTCGCTGCGCTCGCCGAATCGCGCGACAACGAAACGGGTG
CCCACATTATTCGCACGCAGGAATACGTTAAGGCATTGGCAGAGCATTTAGCCCAAGACCCACAACACGTTTCTCTGCTG
ACGCCAACTTACATCGATTTACTCTATAAATCCGCCCCCCTCCACGATGTGGGAAAAGTCGGTATCCCCGACAGTGTGCT
GCTCAAGCCAGGCAAATTAACTGACGAAGAATTTGACATCATGAAAGGTCACCCACAAATTGGCGCTGATGCCCTCTCGA
TTGCTGAAAAACGCATGGGCTCTAGTTCTTTTTTACGAGTGGCTAAAGAGATATCCCTCTCTCACCATGAAAAATGGGAC
GGTTCTGGTTATCCCAATCAACTGGCTGGCGAAGCGATTCCATTATCTGGGCGATTAATGGCGCTCGCCGACGTCTACGA
TGCGCTGATTTCTAAGCGTGTATACAAACCGGCGTTTAGTCATGATAAAGCCAAAGAGATCATTCTTGATGGCAAAGGAA
AGCATTTTGACCCTCAAGTGGTCGACGCCTTTTTAGCGGTAGAAGCACAGTTTTGTCGTATTGCAGCACAATATCAAGAC
TCCGCAACTCATTAG

Upstream 100 bases:

>100_bases
TTCTTTTCTTTGAACAATCAGCGTAATGCTGAAGGTTCAATCAAGCAGAGAACTGGCCAAGTCGCATTTGCTGCGAATGA
TAATAACAAGGGCTTACACA

Downstream 100 bases:

>100_bases
ACCTCTAACATCATGTTAACACCCCAATTTCCCCCGCGGTTTTGGGGTGTTTTATTAGCAAAACTCGCAATTCATTAGCA
CTTCTGTTTCAAATCAAATT

Product: Response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSHYNAPLTFKLYALAAIVFGTYGGRVCPMLATLSPLEVLVHVSITFLAVFAVRHFFFSQHRWVREQKHAQLDTVLFFIG
SLPLAYYYNSQYDFTIDSNLKVLFGMTLFGFFTGLILQLKAKLLRFELLEENATRQDTLSGERQSIIKQLILMMTLLIIA
LTTMLTMVAVKDIFWLEHNPERLLDGSGKISIIKEFIYLGVVLGGYVLAILLLWSKLMKKILLRQEHSLQEVTNGAIGCR
LAVFEHDELGSMAALTNKMLDSLQASQDEVKTTRDVAIVSLAALAESRDNETGAHIIRTQEYVKALAEHLAQDPQHVSLL
TPTYIDLLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFDIMKGHPQIGADALSIAEKRMGSSSFLRVAKEISLSHHEKWD
GSGYPNQLAGEAIPLSGRLMALADVYDALISKRVYKPAFSHDKAKEIILDGKGKHFDPQVVDAFLAVEAQFCRIAAQYQD
SATH

Sequences:

>Translated_484_residues
MSHYNAPLTFKLYALAAIVFGTYGGRVCPMLATLSPLEVLVHVSITFLAVFAVRHFFFSQHRWVREQKHAQLDTVLFFIG
SLPLAYYYNSQYDFTIDSNLKVLFGMTLFGFFTGLILQLKAKLLRFELLEENATRQDTLSGERQSIIKQLILMMTLLIIA
LTTMLTMVAVKDIFWLEHNPERLLDGSGKISIIKEFIYLGVVLGGYVLAILLLWSKLMKKILLRQEHSLQEVTNGAIGCR
LAVFEHDELGSMAALTNKMLDSLQASQDEVKTTRDVAIVSLAALAESRDNETGAHIIRTQEYVKALAEHLAQDPQHVSLL
TPTYIDLLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFDIMKGHPQIGADALSIAEKRMGSSSFLRVAKEISLSHHEKWD
GSGYPNQLAGEAIPLSGRLMALADVYDALISKRVYKPAFSHDKAKEIILDGKGKHFDPQVVDAFLAVEAQFCRIAAQYQD
SATH
>Mature_483_residues
SHYNAPLTFKLYALAAIVFGTYGGRVCPMLATLSPLEVLVHVSITFLAVFAVRHFFFSQHRWVREQKHAQLDTVLFFIGS
LPLAYYYNSQYDFTIDSNLKVLFGMTLFGFFTGLILQLKAKLLRFELLEENATRQDTLSGERQSIIKQLILMMTLLIIAL
TTMLTMVAVKDIFWLEHNPERLLDGSGKISIIKEFIYLGVVLGGYVLAILLLWSKLMKKILLRQEHSLQEVTNGAIGCRL
AVFEHDELGSMAALTNKMLDSLQASQDEVKTTRDVAIVSLAALAESRDNETGAHIIRTQEYVKALAEHLAQDPQHVSLLT
PTYIDLLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFDIMKGHPQIGADALSIAEKRMGSSSFLRVAKEISLSHHEKWDG
SGYPNQLAGEAIPLSGRLMALADVYDALISKRVYKPAFSHDKAKEIILDGKGKHFDPQVVDAFLAVEAQFCRIAAQYQDS
ATH

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 54011; Mature: 53879

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50885 HAMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYNAPLTFKLYALAAIVFGTYGGRVCPMLATLSPLEVLVHVSITFLAVFAVRHFFFSQ
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
HRWVREQKHAQLDTVLFFIGSLPLAYYYNSQYDFTIDSNLKVLFGMTLFGFFTGLILQLK
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AKLLRFELLEENATRQDTLSGERQSIIKQLILMMTLLIIALTTMLTMVAVKDIFWLEHNP
HHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ERLLDGSGKISIIKEFIYLGVVLGGYVLAILLLWSKLMKKILLRQEHSLQEVTNGAIGCR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LAVFEHDELGSMAALTNKMLDSLQASQDEVKTTRDVAIVSLAALAESRDNETGAHIIRTQ
EEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EYVKALAEHLAQDPQHVSLLTPTYIDLLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFDI
HHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCHHHH
MKGHPQIGADALSIAEKRMGSSSFLRVAKEISLSHHEKWDGSGYPNQLAGEAIPLSGRLM
HCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHH
ALADVYDALISKRVYKPAFSHDKAKEIILDGKGKHFDPQVVDAFLAVEAQFCRIAAQYQD
HHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
SATH
CCCC
>Mature Secondary Structure 
SHYNAPLTFKLYALAAIVFGTYGGRVCPMLATLSPLEVLVHVSITFLAVFAVRHFFFSQ
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
HRWVREQKHAQLDTVLFFIGSLPLAYYYNSQYDFTIDSNLKVLFGMTLFGFFTGLILQLK
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AKLLRFELLEENATRQDTLSGERQSIIKQLILMMTLLIIALTTMLTMVAVKDIFWLEHNP
HHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ERLLDGSGKISIIKEFIYLGVVLGGYVLAILLLWSKLMKKILLRQEHSLQEVTNGAIGCR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LAVFEHDELGSMAALTNKMLDSLQASQDEVKTTRDVAIVSLAALAESRDNETGAHIIRTQ
EEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EYVKALAEHLAQDPQHVSLLTPTYIDLLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFDI
HHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCHHHH
MKGHPQIGADALSIAEKRMGSSSFLRVAKEISLSHHEKWDGSGYPNQLAGEAIPLSGRLM
HCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHH
ALADVYDALISKRVYKPAFSHDKAKEIILDGKGKHFDPQVVDAFLAVEAQFCRIAAQYQD
HHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
SATH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]