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The map label for this gene is pflA [H]

Identifier: 27365428

GI number: 27365428

Start: 2092282

End: 2093022

Strand: Reverse

Name: pflA [H]

Synonym: VV1_2095

Alternate gene names: 27365428

Gene position: 2093022-2092282 (Counterclockwise)

Preceding gene: 326423939

Following gene: 27365427

Centisome position: 63.78

GC content: 46.29

Gene sequence:

>741_bases
ATGACAACAACGGGTCGTATTCACTCTTTTGAATCCTGCGGAACGGTTGACGGACCAGGTATTCGTTTTATTGTTTTTAT
GCAGGGCTGTCTGATGCGTTGTATGTATTGCCACAACCGCGATACATGGGACACGCACGACGGTAAAGAGGTCACCGTAG
AAGAGATCATCAAGGAAGCGAAATCTTATCGTCATTTTATGAACGCCTCTGGTGGTGGTATCACTTGTTCCGGTGGCGAA
GCGATGCTGCAACCTGAATTCGTGCGCGATCTATTCCGCGCGGCGCATGCTGAAGGTATTCACACTTGTTTGGATACCAA
CGGTTATATCCGTAAGCACACTGACGTTGTTGACGAAGTATTAGAAGCAACCGATTTGGTGATGCTCGATATCAAACACA
TGAAAGATGAAATCCATCATGATTTCATCGGCGTTTCCAACCGTCGTACTCTCGATTTTGCTCGCTACTTGCATAAGATT
GGGCAAACAACGTGGATTCGCTACGTTGTGGTACCGGGTTACACGGATGATCCTGAAGCGGCTCATATGCTTGGCGAGTT
CATCAAAGACATGGACAACATCGAGAAGGTCGAATTGCTTCCTTACCATAAGCTTGGTGCTCACAAATGGGAAGCGTTGG
GTCTAGAGTATCCACTGGAAGGGGTGAACCCACCACCAAAAGAGACGATGGATAATATTGTTTCTATCCTTTCGCAATAC
CATAGCAATGTGAAATACTAA

Upstream 100 bases:

>100_bases
TTCTTGCTCAGACGCTGATTCCCGTGTTTTGTATACTGTTTTAACCCCAGTTTTTGTAATAAAATAACAGCCGAAATTTG
ATAAGAATTGAGAGCTAGAC

Downstream 100 bases:

>100_bases
GCACCGTATTCGTTATTCAATACCCACTTTAAGTGGGTATTGTTTTATCTACAGGTATGAATGACATGGATGTATTTGCC
TTTCTTTGCTTAGGCGTTAT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTTTGRIHSFESCGTVDGPGIRFIVFMQGCLMRCMYCHNRDTWDTHDGKEVTVEEIIKEAKSYRHFMNASGGGITCSGGE
AMLQPEFVRDLFRAAHAEGIHTCLDTNGYIRKHTDVVDEVLEATDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKI
GQTTWIRYVVVPGYTDDPEAAHMLGEFIKDMDNIEKVELLPYHKLGAHKWEALGLEYPLEGVNPPPKETMDNIVSILSQY
HSNVKY

Sequences:

>Translated_246_residues
MTTTGRIHSFESCGTVDGPGIRFIVFMQGCLMRCMYCHNRDTWDTHDGKEVTVEEIIKEAKSYRHFMNASGGGITCSGGE
AMLQPEFVRDLFRAAHAEGIHTCLDTNGYIRKHTDVVDEVLEATDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKI
GQTTWIRYVVVPGYTDDPEAAHMLGEFIKDMDNIEKVELLPYHKLGAHKWEALGLEYPLEGVNPPPKETMDNIVSILSQY
HSNVKY
>Mature_245_residues
TTTGRIHSFESCGTVDGPGIRFIVFMQGCLMRCMYCHNRDTWDTHDGKEVTVEEIIKEAKSYRHFMNASGGGITCSGGEA
MLQPEFVRDLFRAAHAEGIHTCLDTNGYIRKHTDVVDEVLEATDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIG
QTTWIRYVVVPGYTDDPEAAHMLGEFIKDMDNIEKVELLPYHKLGAHKWEALGLEYPLEGVNPPPKETMDNIVSILSQYH
SNVKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=68.2926829268293, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1790389, Length=252, Percent_Identity=24.6031746031746, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510931, Length=170, Percent_Identity=31.7647058823529, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28046; Mature: 27915

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTGRIHSFESCGTVDGPGIRFIVFMQGCLMRCMYCHNRDTWDTHDGKEVTVEEIIKEA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
KSYRHFMNASGGGITCSGGEAMLQPEFVRDLFRAAHAEGIHTCLDTNGYIRKHTDVVDEV
HHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEATDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIGQTTWIRYVVVPGYTDDPEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHH
AHMLGEFIKDMDNIEKVELLPYHKLGAHKWEALGLEYPLEGVNPPPKETMDNIVSILSQY
HHHHHHHHHHHHCCCHHHCCCCHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HSNVKY
HHCCCC
>Mature Secondary Structure 
TTTGRIHSFESCGTVDGPGIRFIVFMQGCLMRCMYCHNRDTWDTHDGKEVTVEEIIKEA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
KSYRHFMNASGGGITCSGGEAMLQPEFVRDLFRAAHAEGIHTCLDTNGYIRKHTDVVDEV
HHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEATDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIGQTTWIRYVVVPGYTDDPEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHH
AHMLGEFIKDMDNIEKVELLPYHKLGAHKWEALGLEYPLEGVNPPPKETMDNIVSILSQY
HHHHHHHHHHHHCCCHHHCCCCHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HSNVKY
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]