Click here to switch to the map view.

The map label for this gene is hsdM [H]

Identifier: 27365370

GI number: 27365370

Start: 2022497

End: 2023987

Strand: Reverse

Name: hsdM [H]

Synonym: VV1_2031

Alternate gene names: 27365370

Gene position: 2023987-2022497 (Counterclockwise)

Preceding gene: 326423924

Following gene: 27365369

Centisome position: 61.67

GC content: 46.55

Gene sequence:

>1491_bases
ATGTCAATCAGTTCAGTTATCAAGTCTATTCAAGACATCATGCGTAAAGATGCGGGTGTTGATGGTGACGCGCAGCGTCT
AGGCCAAATGTCGTGGCTTTTATTCCTTAAAGTGTTTGATGCCCAAGAGGAAGAGCTAGAGTTCGAGTTAGATGATTACC
GCGAACCCATCCCAGCCAAGTACCTTTGGCGTAACTGGGCGGCAGATAACCAAGGCATTACAGGTGACGAGCTGCTGGAA
TTTATCAATGATGACCTATTCCCGACGTTAAAAAACCTAACCGCACCGAAAGACACCAACCCACGCGGTTTTGTGGTGAA
AGAAGCTTTTAGTGATGCCTTCAACTACATGAAAAATGGCACACTGCTTCGTCAAGTGATTAACAAGCTCAACGAAATCG
ACTTTACTGACTCTAAAGAACGCCACCTGTTTGGTGATATCTATGAACAAATCCTACGCGACCTACAAAGTGCAGGTAAT
GCGGGTGAGTTCTATACCCCTCGCGCGGTGACTCGTTTTATCGTCAACAGACTGGATCCAAAGCTAGGTGAGCAAATTAT
GGATCCCGCTTGCGGCACGGGCGGTTTCTTAGCTTGCTCGTTCGATCACGTCAAAGAGAACTACGTGACAAGCGCAGCCG
ACCACCAAACACTGCAAAAGCAAATTCATGGTGTAGAGAAGAAACAACTGCCACATTTGCTGTGTATCACCAATATGATG
CTCCACGGCATCGAAGTCCCTGTGCAGATTAAGCATGGCAACACGCTCAATAAGCCGCTTTCAAGTTGGGATAGCAATAT
CAACGTGATTGCGACTAACCCACCGTTTGGCGGTACAGAAGAGGACGGGATTGAAAAGAACTTCCCTGCCGAAATGCAAA
CTCGTGAAACGGCCGATTTGTTCTTGCAACTTATTGTCGAAGTATTGGATAAAGAGGGTCGCGCAGGTGTAGTGTTGCCT
GATGGCACCTTGTTTGGTGAAGGTGTAAAAACCAAAATCAAAAAGATGCTGACGGAAGAGTGTAACCTGCACACGATTGT
TCGCTTACCTAATGGCGTGTTCAACCCATACACAGGTATTAAGACCAACATTCTGTTCTTTACCAAAGGCCAACCGACCA
AAGAAGTGTGGTTCTATGAGCACCCTTACCCAGAAGGCGTGAAGAACTACAGCAAAACCAAACCGATGAAGTTTGAGGAG
TTCCAGCAAGAGATCGATTGGTGGGGCAACGAGGCAGATGGATTCGCTAGCCGCATCGAGACCAAGCAAGCTTGGAAAGT
GTCGATTGAAGATATTATCGAGCGCAACTTTAACCTTGATATTAAAAACCCGTATCAGGACGAAGTCGTCAGCCATGACC
CTGAAGAGTTGCTGGCGAACTATCAGCAGCAACAACAAGACATCAAAGCACTGCGTGACCAACTGAAAAGCATTCTCGGT
GATGCTCTTAGCTCTACCATTCAAGGTAAAAAGCAAGGTGGAGAACAATAA

Upstream 100 bases:

>100_bases
ACAATTACAAACATAGTTTCATAGCGATAAGTTCATTTTCTTCGACCCATTTCTGCTATAATCAGCCCCAATTTTTTCAG
TCAATTAGCAGTAGAACTCC

Downstream 100 bases:

>100_bases
TGGCAGTCGAAAACCTGATTACCGAGCATATTGATATCTGGACTTCGGCAGTAAAGACCAAATCGACCTCTGGTCGCGGT
AGCAGCAAGAAACTAGAGCT

Product: type I restriction-modification system, DNA-methyltransferase subunit M

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSISSVIKSIQDIMRKDAGVDGDAQRLGQMSWLLFLKVFDAQEEELEFELDDYREPIPAKYLWRNWAADNQGITGDELLE
FINDDLFPTLKNLTAPKDTNPRGFVVKEAFSDAFNYMKNGTLLRQVINKLNEIDFTDSKERHLFGDIYEQILRDLQSAGN
AGEFYTPRAVTRFIVNRLDPKLGEQIMDPACGTGGFLACSFDHVKENYVTSAADHQTLQKQIHGVEKKQLPHLLCITNMM
LHGIEVPVQIKHGNTLNKPLSSWDSNINVIATNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLDKEGRAGVVLP
DGTLFGEGVKTKIKKMLTEECNLHTIVRLPNGVFNPYTGIKTNILFFTKGQPTKEVWFYEHPYPEGVKNYSKTKPMKFEE
FQQEIDWWGNEADGFASRIETKQAWKVSIEDIIERNFNLDIKNPYQDEVVSHDPEELLANYQQQQQDIKALRDQLKSILG
DALSSTIQGKKQGGEQ

Sequences:

>Translated_496_residues
MSISSVIKSIQDIMRKDAGVDGDAQRLGQMSWLLFLKVFDAQEEELEFELDDYREPIPAKYLWRNWAADNQGITGDELLE
FINDDLFPTLKNLTAPKDTNPRGFVVKEAFSDAFNYMKNGTLLRQVINKLNEIDFTDSKERHLFGDIYEQILRDLQSAGN
AGEFYTPRAVTRFIVNRLDPKLGEQIMDPACGTGGFLACSFDHVKENYVTSAADHQTLQKQIHGVEKKQLPHLLCITNMM
LHGIEVPVQIKHGNTLNKPLSSWDSNINVIATNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLDKEGRAGVVLP
DGTLFGEGVKTKIKKMLTEECNLHTIVRLPNGVFNPYTGIKTNILFFTKGQPTKEVWFYEHPYPEGVKNYSKTKPMKFEE
FQQEIDWWGNEADGFASRIETKQAWKVSIEDIIERNFNLDIKNPYQDEVVSHDPEELLANYQQQQQDIKALRDQLKSILG
DALSSTIQGKKQGGEQ
>Mature_495_residues
SISSVIKSIQDIMRKDAGVDGDAQRLGQMSWLLFLKVFDAQEEELEFELDDYREPIPAKYLWRNWAADNQGITGDELLEF
INDDLFPTLKNLTAPKDTNPRGFVVKEAFSDAFNYMKNGTLLRQVINKLNEIDFTDSKERHLFGDIYEQILRDLQSAGNA
GEFYTPRAVTRFIVNRLDPKLGEQIMDPACGTGGFLACSFDHVKENYVTSAADHQTLQKQIHGVEKKQLPHLLCITNMML
HGIEVPVQIKHGNTLNKPLSSWDSNINVIATNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLDKEGRAGVVLPD
GTLFGEGVKTKIKKMLTEECNLHTIVRLPNGVFNPYTGIKTNILFFTKGQPTKEVWFYEHPYPEGVKNYSKTKPMKFEEF
QQEIDWWGNEADGFASRIETKQAWKVSIEDIIERNFNLDIKNPYQDEVVSHDPEELLANYQQQQQDIKALRDQLKSILGD
ALSSTIQGKKQGGEQ

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=481, Percent_Identity=28.0665280665281, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56429; Mature: 56298

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISSVIKSIQDIMRKDAGVDGDAQRLGQMSWLLFLKVFDAQEEELEFELDDYREPIPAK
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHCCCCCHH
YLWRNWAADNQGITGDELLEFINDDLFPTLKNLTAPKDTNPRGFVVKEAFSDAFNYMKNG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHCC
TLLRQVINKLNEIDFTDSKERHLFGDIYEQILRDLQSAGNAGEFYTPRAVTRFIVNRLDP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCH
KLGEQIMDPACGTGGFLACSFDHVKENYVTSAADHQTLQKQIHGVEKKQLPHLLCITNMM
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
LHGIEVPVQIKHGNTLNKPLSSWDSNINVIATNPPFGGTEEDGIEKNFPAEMQTRETADL
HHCCCCEEEEECCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FLQLIVEVLDKEGRAGVVLPDGTLFGEGVKTKIKKMLTEECNLHTIVRLPNGVFNPYTGI
HHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
KTNILFFTKGQPTKEVWFYEHPYPEGVKNYSKTKPMKFEEFQQEIDWWGNEADGFASRIE
EEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
TKQAWKVSIEDIIERNFNLDIKNPYQDEVVSHDPEELLANYQQQQQDIKALRDQLKSILG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALSSTIQGKKQGGEQ
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SISSVIKSIQDIMRKDAGVDGDAQRLGQMSWLLFLKVFDAQEEELEFELDDYREPIPAK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHCCCCCHH
YLWRNWAADNQGITGDELLEFINDDLFPTLKNLTAPKDTNPRGFVVKEAFSDAFNYMKNG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHCC
TLLRQVINKLNEIDFTDSKERHLFGDIYEQILRDLQSAGNAGEFYTPRAVTRFIVNRLDP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCH
KLGEQIMDPACGTGGFLACSFDHVKENYVTSAADHQTLQKQIHGVEKKQLPHLLCITNMM
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
LHGIEVPVQIKHGNTLNKPLSSWDSNINVIATNPPFGGTEEDGIEKNFPAEMQTRETADL
HHCCCCEEEEECCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FLQLIVEVLDKEGRAGVVLPDGTLFGEGVKTKIKKMLTEECNLHTIVRLPNGVFNPYTGI
HHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
KTNILFFTKGQPTKEVWFYEHPYPEGVKNYSKTKPMKFEEFQQEIDWWGNEADGFASRIE
EEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
TKQAWKVSIEDIIERNFNLDIKNPYQDEVVSHDPEELLANYQQQQQDIKALRDQLKSILG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALSSTIQGKKQGGEQ
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]