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The map label for this gene is hsdS [H]

Identifier: 27365369

GI number: 27365369

Start: 2020815

End: 2022497

Strand: Reverse

Name: hsdS [H]

Synonym: VV1_2030

Alternate gene names: 27365369

Gene position: 2022497-2020815 (Counterclockwise)

Preceding gene: 27365370

Following gene: 27365367

Centisome position: 61.63

GC content: 42.78

Gene sequence:

>1683_bases
ATGGCAGTCGAAAACCTGATTACCGAGCATATTGATATCTGGACTTCGGCAGTAAAGACCAAATCGACCTCTGGTCGCGG
TAGCAGCAAGAAACTAGAGCTTTACGGTGTTAAGAAGCTGCGTGAGCTAATTTTAGAACTAGCAGTTCGCGGCAAACTCG
TTCCTCAAAACCCAAACGATGAACCTGCATCTTTTTTACTTCAACGGATTTCAGAGGAAAAAACGCAGTTAATTAAAGAA
AAAAAAATTACAAAGCCCAAAAAGCTCCCTGCTATTAAAAAACAAGATATAACGTTTGCTTTACCAGAAGGTTGGCAAGC
TTGTTATTTTGGTGATATTTATAGCTTGGTTTATGGGGATAATCTCCCAAAAGCAAAACGTACTGAAAGTGGTGAATTTT
TAGTCTATGGCTCTAATGGTTCTGTAGGGACTCACAATCTTTTTTCTGTTGGTAGCCCTTGTCTCGTTATCGGTCGTAAA
GGATCTGCGGGTGCAATTAATTTAAGTGACCAACCTTGCTGGGTTACAGATGTAGCCTACAGCTTAATACCACCTGTAGG
TATAAGCCTTAAGTATTGTTTTCTACATTTACAAACTTTAGGGTTAGACTCACTCGGAAAGGGTATAAAACCAGGCTTAA
ATAGAAATGAAGCTAACGCGCTAGTAGTTTGTATCCCCCCTTCCGACGAGCAACACCGCATCGTCGCCAAAGTCGATGAG
CTAATGGCGCTCTGCGACCAACTAGAGCAGCAAACCGAAGCCAGTATTGAAGCGCATCAATTGCTGGTAACAACACTGTT
GGACACACTCACCAACTCTGCCGATGCTGACGAGTTAATGCAAAACTGGGCGCGAATCAGTGAGCATTTCGATACCTTAT
TCAGCACTGAAGAGAGTATCGACCAGCTTAAACAAACCATCTTACAACTGGCGGTAATGGGTAAGTTGGTTCCACAAGAT
CCAAGCGATGAGCCTGCTGCTGAGTTACTGAAAAGAATTGCGGATGAAAAAGCGCAGTTAGTCAAAGATAAGAAAATCAA
GAAGCAAAAAGCGCTGCCGCCGATTGCTGAAGATGAAAAGCCGTTTGAACTGCCGAGTGGCTGGGAGTGGTGTCGAATTC
AAGATGTAGCTCTATTTACTACATCGGGTTCTCGAGATTGGGCTAAATATTACTCTGACTCTGGTGCTCTATTTGTCACC
ATGGGCAATTTATCCCGAGGCAGCTACGAGCTGCGTTTAGACAATTTAAGGTTTGTACGCCCACCTAAAGGGGGCGAAGG
CTCTCGAACTAAATTGGAGGCGCGAGATCTATTAATTTCCATAACAGGTGATGTCGGTAACTTGGGATTAATTCCTGAAG
AGTTTGGTGAAGCATACATAAACCAACATACGTGCTTATTACGATTTATGCCAGAATGCCAAGGAAAATACTTCCCTGAT
TTTATGAGGTCTCCACTAGCTAAATATCAGTTTGATGCTCCACAACGAGGTATTAAAAATAGCTTTAGGTTGAGTGATGT
TGGTGAAATGCACTTACCGCTTCCTCCTCTAAATGAACAAGTCCGTATCACAGAAAAAGTTAGTGACTTGCTCAGTATAT
GTGAGCGCTTAAAAGTCAGACTTCGAGAGTCTCAAATCACCCAATTACACCTCACCGATGCAATTGTTGAGCGAGCAGTA
TAA

Upstream 100 bases:

>100_bases
AACAACAAGACATCAAAGCACTGCGTGACCAACTGAAAAGCATTCTCGGTGATGCTCTTAGCTCTACCATTCAAGGTAAA
AAGCAAGGTGGAGAACAATA

Downstream 100 bases:

>100_bases
CTATGAGTCTCTACAATATCGCCAACAAAAACCTCTCAAGCTAACATCGACAACTCTTGCAGCCCTCGGCTGCAAGAGAC
TTCTATCTAGCAACAATACC

Product: Restriction endonuclease S subunit

Products: NA

Alternate protein names: S.EcoEI; Type I restriction enzyme EcoEI specificity protein; S protein [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MAVENLITEHIDIWTSAVKTKSTSGRGSSKKLELYGVKKLRELILELAVRGKLVPQNPNDEPASFLLQRISEEKTQLIKE
KKITKPKKLPAIKKQDITFALPEGWQACYFGDIYSLVYGDNLPKAKRTESGEFLVYGSNGSVGTHNLFSVGSPCLVIGRK
GSAGAINLSDQPCWVTDVAYSLIPPVGISLKYCFLHLQTLGLDSLGKGIKPGLNRNEANALVVCIPPSDEQHRIVAKVDE
LMALCDQLEQQTEASIEAHQLLVTTLLDTLTNSADADELMQNWARISEHFDTLFSTEESIDQLKQTILQLAVMGKLVPQD
PSDEPAAELLKRIADEKAQLVKDKKIKKQKALPPIAEDEKPFELPSGWEWCRIQDVALFTTSGSRDWAKYYSDSGALFVT
MGNLSRGSYELRLDNLRFVRPPKGGEGSRTKLEARDLLISITGDVGNLGLIPEEFGEAYINQHTCLLRFMPECQGKYFPD
FMRSPLAKYQFDAPQRGIKNSFRLSDVGEMHLPLPPLNEQVRITEKVSDLLSICERLKVRLRESQITQLHLTDAIVERAV

Sequences:

>Translated_560_residues
MAVENLITEHIDIWTSAVKTKSTSGRGSSKKLELYGVKKLRELILELAVRGKLVPQNPNDEPASFLLQRISEEKTQLIKE
KKITKPKKLPAIKKQDITFALPEGWQACYFGDIYSLVYGDNLPKAKRTESGEFLVYGSNGSVGTHNLFSVGSPCLVIGRK
GSAGAINLSDQPCWVTDVAYSLIPPVGISLKYCFLHLQTLGLDSLGKGIKPGLNRNEANALVVCIPPSDEQHRIVAKVDE
LMALCDQLEQQTEASIEAHQLLVTTLLDTLTNSADADELMQNWARISEHFDTLFSTEESIDQLKQTILQLAVMGKLVPQD
PSDEPAAELLKRIADEKAQLVKDKKIKKQKALPPIAEDEKPFELPSGWEWCRIQDVALFTTSGSRDWAKYYSDSGALFVT
MGNLSRGSYELRLDNLRFVRPPKGGEGSRTKLEARDLLISITGDVGNLGLIPEEFGEAYINQHTCLLRFMPECQGKYFPD
FMRSPLAKYQFDAPQRGIKNSFRLSDVGEMHLPLPPLNEQVRITEKVSDLLSICERLKVRLRESQITQLHLTDAIVERAV
>Mature_559_residues
AVENLITEHIDIWTSAVKTKSTSGRGSSKKLELYGVKKLRELILELAVRGKLVPQNPNDEPASFLLQRISEEKTQLIKEK
KITKPKKLPAIKKQDITFALPEGWQACYFGDIYSLVYGDNLPKAKRTESGEFLVYGSNGSVGTHNLFSVGSPCLVIGRKG
SAGAINLSDQPCWVTDVAYSLIPPVGISLKYCFLHLQTLGLDSLGKGIKPGLNRNEANALVVCIPPSDEQHRIVAKVDEL
MALCDQLEQQTEASIEAHQLLVTTLLDTLTNSADADELMQNWARISEHFDTLFSTEESIDQLKQTILQLAVMGKLVPQDP
SDEPAAELLKRIADEKAQLVKDKKIKKQKALPPIAEDEKPFELPSGWEWCRIQDVALFTTSGSRDWAKYYSDSGALFVTM
GNLSRGSYELRLDNLRFVRPPKGGEGSRTKLEARDLLISITGDVGNLGLIPEEFGEAYINQHTCLLRFMPECQGKYFPDF
MRSPLAKYQFDAPQRGIKNSFRLSDVGEMHLPLPPLNEQVRITEKVSDLLSICERLKVRLRESQITQLHLTDAIVERAV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 62520; Mature: 62388

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVENLITEHIDIWTSAVKTKSTSGRGSSKKLELYGVKKLRELILELAVRGKLVPQNPND
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
EPASFLLQRISEEKTQLIKEKKITKPKKLPAIKKQDITFALPEGWQACYFGDIYSLVYGD
CHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCC
NLPKAKRTESGEFLVYGSNGSVGTHNLFSVGSPCLVIGRKGSAGAINLSDQPCWVTDVAY
CCCCCCCCCCCCEEEECCCCCCCCCCHHHCCCCEEEEECCCCCCEEECCCCCCCHHHHHH
SLIPPVGISLKYCFLHLQTLGLDSLGKGIKPGLNRNEANALVVCIPPSDEQHRIVAKVDE
HHCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
LMALCDQLEQQTEASIEAHQLLVTTLLDTLTNSADADELMQNWARISEHFDTLFSTEESI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
DQLKQTILQLAVMGKLVPQDPSDEPAAELLKRIADEKAQLVKDKKIKKQKALPPIAEDEK
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PFELPSGWEWCRIQDVALFTTSGSRDWAKYYSDSGALFVTMGNLSRGSYELRLDNLRFVR
CCCCCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCEEEEEECCEEEEC
PPKGGEGSRTKLEARDLLISITGDVGNLGLIPEEFGEAYINQHTCLLRFMPECQGKYFPD
CCCCCCCCCCEEEECEEEEEEECCCCCCCCCHHHHHHHHHCCCHHEEHHCHHHCCCCCCH
FMRSPLAKYQFDAPQRGIKNSFRLSDVGEMHLPLPPLNEQVRITEKVSDLLSICERLKVR
HHHCCHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LRESQITQLHLTDAIVERAV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVENLITEHIDIWTSAVKTKSTSGRGSSKKLELYGVKKLRELILELAVRGKLVPQNPND
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
EPASFLLQRISEEKTQLIKEKKITKPKKLPAIKKQDITFALPEGWQACYFGDIYSLVYGD
CHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCC
NLPKAKRTESGEFLVYGSNGSVGTHNLFSVGSPCLVIGRKGSAGAINLSDQPCWVTDVAY
CCCCCCCCCCCCEEEECCCCCCCCCCHHHCCCCEEEEECCCCCCEEECCCCCCCHHHHHH
SLIPPVGISLKYCFLHLQTLGLDSLGKGIKPGLNRNEANALVVCIPPSDEQHRIVAKVDE
HHCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
LMALCDQLEQQTEASIEAHQLLVTTLLDTLTNSADADELMQNWARISEHFDTLFSTEESI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
DQLKQTILQLAVMGKLVPQDPSDEPAAELLKRIADEKAQLVKDKKIKKQKALPPIAEDEK
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PFELPSGWEWCRIQDVALFTTSGSRDWAKYYSDSGALFVTMGNLSRGSYELRLDNLRFVR
CCCCCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCEEEEEECCEEEEC
PPKGGEGSRTKLEARDLLISITGDVGNLGLIPEEFGEAYINQHTCLLRFMPECQGKYFPD
CCCCCCCCCCEEEECEEEEEEECCCCCCCCCHHHHHHHHHCCCHHEEHHCHHHCCCCCCH
FMRSPLAKYQFDAPQRGIKNSFRLSDVGEMHLPLPPLNEQVRITEKVSDLLSICERLKVR
HHHCCHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LRESQITQLHLTDAIVERAV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]