Click here to switch to the map view.

The map label for this gene is dapE

Identifier: 27365262

GI number: 27365262

Start: 1908998

End: 1910131

Strand: Reverse

Name: dapE

Synonym: VV1_1916

Alternate gene names: 27365262

Gene position: 1910131-1908998 (Counterclockwise)

Preceding gene: 326423907

Following gene: 27365261

Centisome position: 58.2

GC content: 51.59

Gene sequence:

>1134_bases
ATGACAGACAGCCCAGTACTGGCTCTAACCAAAGATCTCATCAGTCGCCAGTCGGTAACCCCCGAAGACGCCGGTTGCCA
AGATGTCATGATAGCGCGCCTAGAGGCTCTTGGTTTTACCATTGAGCGCATGGTGTTTGAAGATACCACCAACTTTTGGG
CTCGCCGTGGCACACAAGCGCCACTCTTCGCCTTTGCAGGCCACACTGACGTGGTGCCCGCTGGAAAATTAGAGCATTGG
CACACACCGCCTTTTGAACCGACCGAAAAAGAAGGCTATTTGTATGGCCGTGGGGCTGCGGATATGAAAGGCTCATTGGC
AGCAATGATCGTCGCGACGGAGCGTTTTATCGCAGAACATCCCGATCATCAGGGTTCTATCGGTTTCCTGATCACCTCAG
ATGAGGAAGGCCCGTTTATCAATGGCACGGTTCGCGTTGTGGAAACGTTGATGGCGCGTGATGAAAACATCGACATGTGT
ATTGTCGGCGAGCCATCAAGCACCGAAGTCGTTGGTGATGTAGTAAAAAATGGTCGTCGTGGTTCGATCACTGGCGACTT
GACCGTCAAAGGGACTCAAGGCCATGTGGCTTACCCTCATCTTGCGGACAACCCAGTGCACAAATCGCTTTTGGCAATTC
ACGCCCTAGCGACCACTGAGTGGGACAAGGGTAATGAATACTTCCCGCCAACCAGCTTCCAGATCCCGAACGTACAAGCG
GGTACGGGTGCGTCCAATGTTATCCCAGGTGAGTTCCACGTTCAGTTCAATTTGCGCTTTAGTACCGAGCTTTGCAACGA
GCGAATTGTGGAACGAGTGACTCAAACACTCGACCAACATGACCTCAATTACGATCTCAAATGGACATACAACGGCGACC
CATTCCTAACTGATACGGGAGCACTTCTGGACGCGGTGGTGGATGCTGTCGATAGCGTCAACCAAACCAAACCCGCGTTA
CTGACCACTGGCGGTACTTCAGACGGACGCTTTATCGCACGAATGGGTGGACAAGTAGTTGAGCTAGGACCGGTGAACGC
CACCATCCATAAAGTGAACGAGTGCGTGAAGATCGATGACTTAGAAAAGCTCACCGATATGTACCAGAAAACATTGGAAA
ACTTATTGGCATGA

Upstream 100 bases:

>100_bases
ATGATCAAACGCCCAATTTTGCGAGTCAATGACCAGCTACACGTTGGCTTTAAAGCAGAACAGTATTCAAAAATTTTCAA
TTAATGAAGGATTATCAAGG

Downstream 100 bases:

>100_bases
ACACCATTGAGCAGCTGACTGGCAGCGAAACGTCTCATTTAGAACACGTTTTAGTGGGGGACAAGTGGTTTTGGCTCCAC
CCTGAGGTGAAAAATGATCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTDSPVLALTKDLISRQSVTPEDAGCQDVMIARLEALGFTIERMVFEDTTNFWARRGTQAPLFAFAGHTDVVPAGKLEHW
HTPPFEPTEKEGYLYGRGAADMKGSLAAMIVATERFIAEHPDHQGSIGFLITSDEEGPFINGTVRVVETLMARDENIDMC
IVGEPSSTEVVGDVVKNGRRGSITGDLTVKGTQGHVAYPHLADNPVHKSLLAIHALATTEWDKGNEYFPPTSFQIPNVQA
GTGASNVIPGEFHVQFNLRFSTELCNERIVERVTQTLDQHDLNYDLKWTYNGDPFLTDTGALLDAVVDAVDSVNQTKPAL
LTTGGTSDGRFIARMGGQVVELGPVNATIHKVNECVKIDDLEKLTDMYQKTLENLLA

Sequences:

>Translated_377_residues
MTDSPVLALTKDLISRQSVTPEDAGCQDVMIARLEALGFTIERMVFEDTTNFWARRGTQAPLFAFAGHTDVVPAGKLEHW
HTPPFEPTEKEGYLYGRGAADMKGSLAAMIVATERFIAEHPDHQGSIGFLITSDEEGPFINGTVRVVETLMARDENIDMC
IVGEPSSTEVVGDVVKNGRRGSITGDLTVKGTQGHVAYPHLADNPVHKSLLAIHALATTEWDKGNEYFPPTSFQIPNVQA
GTGASNVIPGEFHVQFNLRFSTELCNERIVERVTQTLDQHDLNYDLKWTYNGDPFLTDTGALLDAVVDAVDSVNQTKPAL
LTTGGTSDGRFIARMGGQVVELGPVNATIHKVNECVKIDDLEKLTDMYQKTLENLLA
>Mature_376_residues
TDSPVLALTKDLISRQSVTPEDAGCQDVMIARLEALGFTIERMVFEDTTNFWARRGTQAPLFAFAGHTDVVPAGKLEHWH
TPPFEPTEKEGYLYGRGAADMKGSLAAMIVATERFIAEHPDHQGSIGFLITSDEEGPFINGTVRVVETLMARDENIDMCI
VGEPSSTEVVGDVVKNGRRGSITGDLTVKGTQGHVAYPHLADNPVHKSLLAIHALATTEWDKGNEYFPPTSFQIPNVQAG
TGASNVIPGEFHVQFNLRFSTELCNERIVERVTQTLDQHDLNYDLKWTYNGDPFLTDTGALLDAVVDAVDSVNQTKPALL
TTGGTSDGRFIARMGGQVVELGPVNATIHKVNECVKIDDLEKLTDMYQKTLENLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=374, Percent_Identity=64.7058823529412, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1790395, Length=205, Percent_Identity=29.2682926829268, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17551016, Length=217, Percent_Identity=28.110599078341, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_VIBVU (Q8DBA6)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760790.1
- ProteinModelPortal:   Q8DBA6
- SMR:   Q8DBA6
- MEROPS:   M20.010
- GeneID:   1178816
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1916
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   VVUL216895:VV1_1916-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41227; Mature: 41096

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSPVLALTKDLISRQSVTPEDAGCQDVMIARLEALGFTIERMVFEDTTNFWARRGTQA
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLFAFAGHTDVVPAGKLEHWHTPPFEPTEKEGYLYGRGAADMKGSLAAMIVATERFIAEH
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHC
PDHQGSIGFLITSDEEGPFINGTVRVVETLMARDENIDMCIVGEPSSTEVVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCC
GSITGDLTVKGTQGHVAYPHLADNPVHKSLLAIHALATTEWDKGNEYFPPTSFQIPNVQA
CCEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCC
GTGASNVIPGEFHVQFNLRFSTELCNERIVERVTQTLDQHDLNYDLKWTYNGDPFLTDTG
CCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECHH
ALLDAVVDAVDSVNQTKPALLTTGGTSDGRFIARMGGQVVELGPVNATIHKVNECVKIDD
HHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHCCCEEEEECCCCHHHHHHHHHHCCCH
LEKLTDMYQKTLENLLA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSPVLALTKDLISRQSVTPEDAGCQDVMIARLEALGFTIERMVFEDTTNFWARRGTQA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLFAFAGHTDVVPAGKLEHWHTPPFEPTEKEGYLYGRGAADMKGSLAAMIVATERFIAEH
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHC
PDHQGSIGFLITSDEEGPFINGTVRVVETLMARDENIDMCIVGEPSSTEVVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCC
GSITGDLTVKGTQGHVAYPHLADNPVHKSLLAIHALATTEWDKGNEYFPPTSFQIPNVQA
CCEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCC
GTGASNVIPGEFHVQFNLRFSTELCNERIVERVTQTLDQHDLNYDLKWTYNGDPFLTDTG
CCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECHH
ALLDAVVDAVDSVNQTKPALLTTGGTSDGRFIARMGGQVVELGPVNATIHKVNECVKIDD
HHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHCCCEEEEECCCCHHHHHHHHHHCCCH
LEKLTDMYQKTLENLLA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA