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The map label for this gene is ebgR [H]

Identifier: 27365125

GI number: 27365125

Start: 1759978

End: 1760976

Strand: Reverse

Name: ebgR [H]

Synonym: VV1_1769

Alternate gene names: 27365125

Gene position: 1760976-1759978 (Counterclockwise)

Preceding gene: 27365131

Following gene: 27365124

Centisome position: 53.66

GC content: 49.75

Gene sequence:

>999_bases
ATGGCTACGTTAAAAGATATTGCCACGGAAGCGGGCGTTTCCCTGGCCACGGTGTCGCGAGTGCTCAATGAAGACCCGAC
TCTGAGTGTCAAAGAAGAGACCAAACGCCGCATCTTAGAAATTGCGGAGAAATTGGAATACCGAACCAGCAGCGCGAAAA
AGAACAGCGTTGCCGCCGTGCCGGCCCATCATTTCCTGGCATTGTACAACTACAAGCCAGAAGCTGAGATTAACGATCCT
TACTATCTCTCTATTCGCCATGGCATTGAAACCCAGTGTGAAAAACTCGGCATCGATTTAACCAACTGCTACGACAGCAA
AATCGACCCCGACGTGAAAAAGATCACCGGTGTCCTTATGGTTGGCAAAACCACGCCTGCCGCGATGTTGCGCGCCAGCA
AGATGACCGACAACATCTGCTATCTCGATTTTAGCGATGACCATGGCGACTTCGACAGCGTGGATATTGATCTGGTAAAA
ATCAGTAAAGAAGTAACGGATTTCTTTATCAGCCAAGGCTTTGACCGCATCGGTTTTATTGGTGGTCAAGATGAACCGGG
CCAAGCCGACATTCGCGAAATGGCCTTTGCTGAATATGGTCAGTTGAAAGGCGTGGTCAATGAAGCGGACATTTATCGTG
GCGATTTCTCCAGTTCATCTGGCTACAAGTTGGCAAAAAGCATGTTAGAACGCAGTGATTATCCGAAGGCGCTGTTTATC
GCTTCCGACTCCATCGCGATTGGCGTGCTGCGCGCTTTGCTCGAACATGGGCTCAACATTCCTCGCGACGTTTCACTGAT
CAGTGTCAACGACATTCCCAATGCCAGATTCACTTTCCCTCCTCTCTCGACGGTGAGAATTCACTCAGAAATGATGGGCA
TTCAAGGCGTTAACTTATTGGTGGAAAAAGCGCGTGATGGCCGAGCACTGCCGCTTAAAGTGTATGTGCCAAGCAAGCTC
AAGCTAAGAGGAACGACGCAGGAGAGGAGCCAAGCATGA

Upstream 100 bases:

>100_bases
GATTTTTACTAAAAAAACGGATGCATCACGCAGCCTCTTGATTACAATAGAGAACAGATGAATTCACTCCGCACAGTGCG
CTGTGCCAACAGGAAGAAGA

Downstream 100 bases:

>100_bases
ACCACGAGCAACTGCTTAAAGAGCTGGAAGAAAACGCCTGCCTTGATCTGCATAAACTCTATTACTCCAACCCAGATAAG
CGCTTTTATGCTCTGCTGGC

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKRRILEIAEKLEYRTSSAKKNSVAAVPAHHFLALYNYKPEAEINDP
YYLSIRHGIETQCEKLGIDLTNCYDSKIDPDVKKITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVK
ISKEVTDFFISQGFDRIGFIGGQDEPGQADIREMAFAEYGQLKGVVNEADIYRGDFSSSSGYKLAKSMLERSDYPKALFI
ASDSIAIGVLRALLEHGLNIPRDVSLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLLVEKARDGRALPLKVYVPSKL
KLRGTTQERSQA

Sequences:

>Translated_332_residues
MATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKRRILEIAEKLEYRTSSAKKNSVAAVPAHHFLALYNYKPEAEINDP
YYLSIRHGIETQCEKLGIDLTNCYDSKIDPDVKKITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVK
ISKEVTDFFISQGFDRIGFIGGQDEPGQADIREMAFAEYGQLKGVVNEADIYRGDFSSSSGYKLAKSMLERSDYPKALFI
ASDSIAIGVLRALLEHGLNIPRDVSLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLLVEKARDGRALPLKVYVPSKL
KLRGTTQERSQA
>Mature_331_residues
ATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKRRILEIAEKLEYRTSSAKKNSVAAVPAHHFLALYNYKPEAEINDPY
YLSIRHGIETQCEKLGIDLTNCYDSKIDPDVKKITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVKI
SKEVTDFFISQGFDRIGFIGGQDEPGQADIREMAFAEYGQLKGVVNEADIYRGDFSSSSGYKLAKSMLERSDYPKALFIA
SDSIAIGVLRALLEHGLNIPRDVSLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLLVEKARDGRALPLKVYVPSKLK
LRGTTQERSQA

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=75.2293577981651, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=28.3582089552239, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790194, Length=316, Percent_Identity=26.5822784810127, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=27.1386430678466, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=23.8372093023256, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788474, Length=319, Percent_Identity=25.0783699059561, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789202, Length=353, Percent_Identity=24.929178470255, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=27.9365079365079, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=23.8938053097345, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36732; Mature: 36601

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKRRILEIAEKLEYRTSSAKKNSVAAV
CCCHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PAHHFLALYNYKPEAEINDPYYLSIRHGIETQCEKLGIDLTNCYDSKIDPDVKKITGVLM
CHHHEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHCCCCCCCCCHHHHEEEEE
VGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVKISKEVTDFFISQGFDRIGFI
ECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHEEC
GGQDEPGQADIREMAFAEYGQLKGVVNEADIYRGDFSSSSGYKLAKSMLERSDYPKALFI
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRALLEHGLNIPRDVSLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLL
ECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCHHHH
VEKARDGRALPLKVYVPSKLKLRGTTQERSQA
HHHCCCCCEEEEEEECCCCEEEECCCHHHCCC
>Mature Secondary Structure 
ATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKRRILEIAEKLEYRTSSAKKNSVAAV
CCHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PAHHFLALYNYKPEAEINDPYYLSIRHGIETQCEKLGIDLTNCYDSKIDPDVKKITGVLM
CHHHEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHCCCCCCCCCHHHHEEEEE
VGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVKISKEVTDFFISQGFDRIGFI
ECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHEEC
GGQDEPGQADIREMAFAEYGQLKGVVNEADIYRGDFSSSSGYKLAKSMLERSDYPKALFI
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRALLEHGLNIPRDVSLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLL
ECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCHHHH
VEKARDGRALPLKVYVPSKLKLRGTTQERSQA
HHHCCCCCEEEEEEECCCCEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]