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The map label for this gene is gshB [H]

Identifier: 27364901

GI number: 27364901

Start: 1502071

End: 1503024

Strand: Reverse

Name: gshB [H]

Synonym: VV1_1530

Alternate gene names: 27364901

Gene position: 1503024-1502071 (Counterclockwise)

Preceding gene: 27364902

Following gene: 27364900

Centisome position: 45.8

GC content: 45.91

Gene sequence:

>954_bases
ATGATCAAACTCGGCATCGTAATGGATCCAATTTCGTCCATTAACATTAAAAAAGATTCTAGCTTCGCCATGATGCTAGA
AGCTCAACGTCGCGGTTGGGAAATCCATTATATGGAGATGAATGATCTACACTTAGATCAAGGCATTGCTATTGCTGACA
CCAAAGTGGTGCAACTGAAAGAAGATCCTAATGGTTGGTATGAGTTCACATCAGAACAAACCATCGAGTTGGCGGAACTG
GATGCGGTCTTGATGCGTAAGGATCCTCCATTTGATACGGAGTACATCTACGCGACCTACATTTTAGAGCGTGCGGAAGA
GCAAGGCACCTTGATCGTCAACAAGCCTCAAAGCTTGCGCGATTGCAATGAAAAACTCTTTACCGCTTGGTTCCCAGAAC
TAACGCCAACCACGATCGTGACGCGCAAAGCAGAGAAAATTAAAGCGTTTCGCCAAGAGCATGGCGACATCATTTTAAAA
CCGCTGGATGGTATGGGTGGTGCTTCGATTTTCCGTGTGAAAGAGAATGATCCTAACGTTTCAGTGATCATCGAAACACT
GACCAACCATGGCCAAAATTACGCAATGGCGCAAACTTTCGTGCCTGATATCAGCAACGGTGACAAACGGATTCTGGTGG
TGGATGGCGAACCGATGCCCTATTGCTTAGCACGTATTCCTGCTAAAGGGGAAACTCGTGGTAACTTGGCTGCTGGCGGC
AGCGGTGAACCTCGACCACTGAGCGAAACCGACCTAAAAATTGCAAACGCAGTGGCTCCAACATTAAAAGAAAAAGGGCT
TATTTTTGTTGGCTTGGATGTGATAGGCGATAAACTGACGGAAATTAACGTCACCAGTCCAACCTGTATTCGCGAAATTG
AAGCTGCCTTCGATATCTCGATTACCGGTAAACTAATGGACGCGATTGAACGTCGTTTACAAGCTCAAGCTTAA

Upstream 100 bases:

>100_bases
TGCTAGGACCACGTGTATTGCGCACAGAAACGGCAGCGTTAACGGCAATTACTGCCTTACAGGTTCGTTTCGGCGATCTG
GGATAACACGGAGAACAGTG

Downstream 100 bases:

>100_bases
ACATGAGTGTTGCAGAGCCAGCGATGGCTCTGCTGTAACAGATTGGAGTCCTGTATGAACCTGACAAATCATTTTCTGGT
TGCGATGCCGGGTATGAAAG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGWEIHYMEMNDLHLDQGIAIADTKVVQLKEDPNGWYEFTSEQTIELAEL
DAVLMRKDPPFDTEYIYATYILERAEEQGTLIVNKPQSLRDCNEKLFTAWFPELTPTTIVTRKAEKIKAFRQEHGDIILK
PLDGMGGASIFRVKENDPNVSVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
SGEPRPLSETDLKIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDISITGKLMDAIERRLQAQA

Sequences:

>Translated_317_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGWEIHYMEMNDLHLDQGIAIADTKVVQLKEDPNGWYEFTSEQTIELAEL
DAVLMRKDPPFDTEYIYATYILERAEEQGTLIVNKPQSLRDCNEKLFTAWFPELTPTTIVTRKAEKIKAFRQEHGDIILK
PLDGMGGASIFRVKENDPNVSVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
SGEPRPLSETDLKIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDISITGKLMDAIERRLQAQA
>Mature_317_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGWEIHYMEMNDLHLDQGIAIADTKVVQLKEDPNGWYEFTSEQTIELAEL
DAVLMRKDPPFDTEYIYATYILERAEEQGTLIVNKPQSLRDCNEKLFTAWFPELTPTTIVTRKAEKIKAFRQEHGDIILK
PLDGMGGASIFRVKENDPNVSVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
SGEPRPLSETDLKIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDISITGKLMDAIERRLQAQA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=316, Percent_Identity=74.3670886075949, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35296; Mature: 35296

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGWEIHYMEMNDLHLDQGIAIADTKVVQLK
CEEEEEEECCCCCCEEECCCCEEEEEEHHHCCCEEEEEEECCEECCCCEEEECEEEEEEE
EDPNGWYEFTSEQTIELAELDAVLMRKDPPFDTEYIYATYILERAEEQGTLIVNKPQSLR
ECCCCCEEECCCCEEEHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
DCNEKLFTAWFPELTPTTIVTRKAEKIKAFRQEHGDIILKPLDGMGGASIFRVKENDPNV
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCE
SVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEECCC
SGEPRPLSETDLKIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDIS
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHEEEE
ITGKLMDAIERRLQAQA
EHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGWEIHYMEMNDLHLDQGIAIADTKVVQLK
CEEEEEEECCCCCCEEECCCCEEEEEEHHHCCCEEEEEEECCEECCCCEEEECEEEEEEE
EDPNGWYEFTSEQTIELAELDAVLMRKDPPFDTEYIYATYILERAEEQGTLIVNKPQSLR
ECCCCCEEECCCCEEEHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
DCNEKLFTAWFPELTPTTIVTRKAEKIKAFRQEHGDIILKPLDGMGGASIFRVKENDPNV
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCE
SVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEECCC
SGEPRPLSETDLKIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDIS
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHEEEE
ITGKLMDAIERRLQAQA
EHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]