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The map label for this gene is tpiA [H]

Identifier: 27364742

GI number: 27364742

Start: 1327339

End: 1328109

Strand: Reverse

Name: tpiA [H]

Synonym: VV1_1343

Alternate gene names: 27364742

Gene position: 1328109-1327339 (Counterclockwise)

Preceding gene: 27364745

Following gene: 27364741

Centisome position: 40.47

GC content: 48.64

Gene sequence:

>771_bases
ATGCGTCGTCCTGTAGTGATGGGTAACTGGAAACTTAACGGCAGCAAAACTATGGTTGCTGAACTGCTAACTGGTCTAAA
CGCTGAACTAGAAGGCGTTGAAGGTGTAGACGTAGCGGTAGCGCCGCCAGCACTTTACATCGACCTTGCAGAGCGCCTAA
TCGCTGAAGGTGGCAACAAGATCATTCTTGGTGCACAAAACACTGACATCAACAACAGCGGTGCATTCACTGGCGACATG
TCTCCTGCAATGCTAAAAGACTTTGGTGCTACTCACATCATTATTGGTCACTCTGAGCGTCGTGAATACCACAATGAATC
AGATGAGTTCGTAGCGAAGAAGTTTGCATTCCTGAAAGAAAATGGTCTAAAACCAGTTTTCTGTATCGGTGAAACTGAAG
CGCAAAACGAAGCAGGCGAAACTGAAGCCGTTTGTGCACGTCAAATCAACGCAGTGATCGACGCATACGGTGTTGAAGCG
CTAAACGGTGCCATCATCGCTTACGAACCAATCTGGGCGATCGGTACAGGTAAAGCTGCAACAGCAGAAGATGCACAGCG
TATCCACGCCTCTATCCGTGCGATGATCGCAGCAAAAGATGCTGCAGTAGCAGAGCAAGTGATCATCCAATACGGTGGTT
CTGTTAAGCCAGAAAACGCGGAAGCTTACTTCGCACAACCAGACATCGATGGTGCTCTTGTTGGTGGCGCATCTCTAGAT
GCTAAGAGCTTCGCAGCGATCGCGAAAGCAGCAGCGAAAATGAAAGCGTAA

Upstream 100 bases:

>100_bases
ACATATTTCTGTTATATTCCTACGTAGATTTTTTACATAAATTGGCTTCATTGGTTCGCCTGTGAAGACTTCGATTCAAT
AAAGATATGGAGATATTCCT

Downstream 100 bases:

>100_bases
TTCAACGCCATCCTATCTTTGTATTTAGGGCGTATTTCTTCGGGAATACGCCCTTTATTTTAGCCTCAACATTTCTCGAA
TTTGGTTTTGCCCTATTCTG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRRPVVMGNWKLNGSKTMVAELLTGLNAELEGVEGVDVAVAPPALYIDLAERLIAEGGNKIILGAQNTDINNSGAFTGDM
SPAMLKDFGATHIIIGHSERREYHNESDEFVAKKFAFLKENGLKPVFCIGETEAQNEAGETEAVCARQINAVIDAYGVEA
LNGAIIAYEPIWAIGTGKAATAEDAQRIHASIRAMIAAKDAAVAEQVIIQYGGSVKPENAEAYFAQPDIDGALVGGASLD
AKSFAAIAKAAAKMKA

Sequences:

>Translated_256_residues
MRRPVVMGNWKLNGSKTMVAELLTGLNAELEGVEGVDVAVAPPALYIDLAERLIAEGGNKIILGAQNTDINNSGAFTGDM
SPAMLKDFGATHIIIGHSERREYHNESDEFVAKKFAFLKENGLKPVFCIGETEAQNEAGETEAVCARQINAVIDAYGVEA
LNGAIIAYEPIWAIGTGKAATAEDAQRIHASIRAMIAAKDAAVAEQVIIQYGGSVKPENAEAYFAQPDIDGALVGGASLD
AKSFAAIAKAAAKMKA
>Mature_256_residues
MRRPVVMGNWKLNGSKTMVAELLTGLNAELEGVEGVDVAVAPPALYIDLAERLIAEGGNKIILGAQNTDINNSGAFTGDM
SPAMLKDFGATHIIIGHSERREYHNESDEFVAKKFAFLKENGLKPVFCIGETEAQNEAGETEAVCARQINAVIDAYGVEA
LNGAIIAYEPIWAIGTGKAATAEDAQRIHASIRAMIAAKDAAVAEQVIIQYGGSVKPENAEAYFAQPDIDGALVGGASLD
AKSFAAIAKAAAKMKA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=239, Percent_Identity=43.9330543933054, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4507645, Length=239, Percent_Identity=43.9330543933054, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=67.7290836653386, Blast_Score=337, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17536593, Length=239, Percent_Identity=48.5355648535565, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=240, Percent_Identity=46.6666666666667, Blast_Score=196, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28572008, Length=240, Percent_Identity=43.75, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572006, Length=240, Percent_Identity=43.75, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572004, Length=240, Percent_Identity=43.75, Blast_Score=177, Evalue=6e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26948; Mature: 26948

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPVVMGNWKLNGSKTMVAELLTGLNAELEGVEGVDVAVAPPALYIDLAERLIAEGGNK
CCCCEEEECEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCE
IILGAQNTDINNSGAFTGDMSPAMLKDFGATHIIIGHSERREYHNESDEFVAKKFAFLKE
EEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHCCCHHHHHHHHHHHHHH
NGLKPVFCIGETEAQNEAGETEAVCARQINAVIDAYGVEALNGAIIAYEPIWAIGTGKAA
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCEEEEECCCCC
TAEDAQRIHASIRAMIAAKDAAVAEQVIIQYGGSVKPENAEAYFAQPDIDGALVGGASLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEECCCCCC
AKSFAAIAKAAAKMKA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRPVVMGNWKLNGSKTMVAELLTGLNAELEGVEGVDVAVAPPALYIDLAERLIAEGGNK
CCCCEEEECEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCE
IILGAQNTDINNSGAFTGDMSPAMLKDFGATHIIIGHSERREYHNESDEFVAKKFAFLKE
EEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHCCCHHHHHHHHHHHHHH
NGLKPVFCIGETEAQNEAGETEAVCARQINAVIDAYGVEALNGAIIAYEPIWAIGTGKAA
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCEEEEECCCCC
TAEDAQRIHASIRAMIAAKDAAVAEQVIIQYGGSVKPENAEAYFAQPDIDGALVGGASLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEECCCCCC
AKSFAAIAKAAAKMKA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]