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The map label for this gene is purH [H]

Identifier: 27364630

GI number: 27364630

Start: 1206350

End: 1207942

Strand: Reverse

Name: purH [H]

Synonym: VV1_1227

Alternate gene names: 27364630

Gene position: 1207942-1206350 (Counterclockwise)

Preceding gene: 27364632

Following gene: 27364629

Centisome position: 36.81

GC content: 51.79

Gene sequence:

>1593_bases
ATGAACAACGCTCGTCCTATTCGCCGTGCGCTTATCAGCGTATCAGACAAAACCGGTATTGTTGAGTTTGCGCAAGCTCT
TGCTGAGCGCGGTGTCGATATCTTATCGACTGGCGGTACTGCTCGTTTACTTGCAGAGCAAGGCATCGCAGTCACTGAAG
TCTCTGACTACACTGGTTTCCCAGAAATGATGGACGGTCGCGTTAAAACGCTTCACCCGAAAGTTCACGGTGGTGTGCTC
GGTCGTCGTGGCCAAGACGATGAGGTCATGGCAAAACACGGCATTAACCCGATCGACATGGTGGTGGTTAACCTCTACCC
ATTCGCAGAAACTGTCGCTAAAGAAGGCTGCACACTGGCTGACGCGGTTGAAAACATCGACATCGGTGGCCCAACCATGG
TTCGCTCTGCGGCGAAAAACCATAAAGACGTGACTATCGTTGTCAACGCTTCAGACTACCATCGCGTCATCACAGAAATG
GACGCCAACGACACGTCGCTTACTCTAGAAACGCGTTTCGACCTCGCTATCGCAGCCTTTGAACACACCGCTGCTTACGA
CGGCATGATCGCGAACTACTTCGGCACTATGGTTCCTTCATACGGAGAGAACAAAGAAGGTGATGAAGAGAGCAAATTCC
CGCGCACTTTCAACCAGCAGTTCATCAAAAAACAAGACATGCGCTACGGTGAGAACAGCCACCAAGCAGCCGCTTTCTAT
GTTGAAGCCAACCCACAAGAAGCATCGGTTGCTACCGCTCGCCAAATCCAAGGCAAAGCCCTTTCTTACAACAACATCGC
AGACACGGACGCAGCGCTTGAGTGTGTGAAAGAGTTTAATGAGCCAGCTTGTGTCATTGTTAAGCACGCTAACCCATGTG
GCGTAGCGCTAGGTAAAGACATCCTAGAGGCGTACAACCGCGCTTACCAAACCGATCCGACATCCGCATTTGGCGGCATC
ATCGCCTTCAACCAAGAGCTAGACGCAGAAACGGCCACCGCTATTGTTGAGCGCCAATTTGTTGAAGTCATCATTGCCCC
TTCGGTATCGGCTGAAGCGATGGAAGTGGTGGCAGCGAAGAAAAACGTTCGTCTGCTTGAGTGTGGCGAGTGGACAACGA
AGACAACGGGCTTTGACGTGAAACGCGTGAACGGCGGCCTACTGGTTCAAGATCGCGACCAAGGCATGGTCAGCCTAGAT
GACCTTAAAGTCGTGTCTAAGCGCCAGCCAACGGAAGAAGAACTCAAAGACGCGTTATTCTGCTGGAAAGTGGCGAAGTA
CGTAAAATCGAACGCGATTGTTTACTCTAAAGGCGATATGACTATTGGTGTGGGCGCTGGCCAAATGAGCCGCGTCTACT
CAGCGAAGATTGCTGGCATTAAAGCGGCAGACGAAGGCCTACAAGTTGAGGGTTGCGTCATGGCCTCTGATGCCTTCTTC
CCATTCCGTGACGGTATTGATGCAGCAGCAGAAGCTGGCATTAAATGTGTAATTCAGCCTGGTGGTTCGATGCGTGATGA
CGAAGTGATTGCCGCCGCAGATGAACACGGTATGGCGATGATTTTTACGGGCATGCGCCACTTCCGCCACTAG

Upstream 100 bases:

>100_bases
TTTCCAAAACTGGCCAATATTTTACCCCAAGAGGTAAAAACAAGTTCATTTTTGGCAAATATCTTTAGTTTAACTCCGTG
AATTTGAGGAAATTGGAAGC

Downstream 100 bases:

>100_bases
TTTTTATCAGAGGTGCCTTATCAGGCACCTCTTTTTGCAAGATTTGAGGATGATGAGATGAATGTATTGATAATTGGTTC
CGGCGGTCGTGAACACGCTC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEQGIAVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDEVMAKHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNASDYHRVITEM
DANDTSLTLETRFDLAIAAFEHTAAYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFIKKQDMRYGENSHQAAAFY
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKDILEAYNRAYQTDPTSAFGGI
IAFNQELDAETATAIVERQFVEVIIAPSVSAEAMEVVAAKKNVRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSLD
DLKVVSKRQPTEEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH

Sequences:

>Translated_530_residues
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEQGIAVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDEVMAKHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNASDYHRVITEM
DANDTSLTLETRFDLAIAAFEHTAAYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFIKKQDMRYGENSHQAAAFY
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKDILEAYNRAYQTDPTSAFGGI
IAFNQELDAETATAIVERQFVEVIIAPSVSAEAMEVVAAKKNVRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSLD
DLKVVSKRQPTEEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
>Mature_530_residues
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEQGIAVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDEVMAKHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNASDYHRVITEM
DANDTSLTLETRFDLAIAAFEHTAAYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFIKKQDMRYGENSHQAAAFY
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKDILEAYNRAYQTDPTSAFGGI
IAFNQELDAETATAIVERQFVEVIIAPSVSAEAMEVVAAKKNVRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSLD
DLKVVSKRQPTEEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=487, Percent_Identity=37.782340862423, Blast_Score=281, Evalue=9e-76,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=78.9077212806026, Blast_Score=879, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=512, Percent_Identity=32.8125, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=39.1304347826087, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985574, Length=342, Percent_Identity=25.4385964912281, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=616, Percent_Identity=32.4675324675325, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6323768, Length=487, Percent_Identity=34.9075975359343, Blast_Score=259, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24649832, Length=613, Percent_Identity=35.2365415986949, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57465; Mature: 57465

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEQGIAVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEHHHHCCEEEEEECCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDEVMAKHGINPIDMVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCHHHHHCCCCHHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNASDYHRVITEMDANDTSLTLETRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCEEEEEH
EHTAAYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFIKKQDMRYGENSHQAAAFY
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ILEAYNRAYQTDPTSAFGGIIAFNQELDAETATAIVERQFVEVIIAPSVSAEAMEVVAAK
HHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHH
KNVRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSLDDLKVVSKRQPTEEELKDALF
CCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MNNARPIRRALISVSDKTGIVEFAQALAERGVDILSTGGTARLLAEQGIAVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEHHHHCCEEEEEECCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDEVMAKHGINPIDMVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCHHHHHCCCCHHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNASDYHRVITEMDANDTSLTLETRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCEEEEEH
EHTAAYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFIKKQDMRYGENSHQAAAFY
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVALGKD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ILEAYNRAYQTDPTSAFGGIIAFNQELDAETATAIVERQFVEVIIAPSVSAEAMEVVAAK
HHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHH
KNVRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSLDDLKVVSKRQPTEEELKDALF
CCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA