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The map label for this gene is plsB

Identifier: 27364581

GI number: 27364581

Start: 1157246

End: 1159675

Strand: Reverse

Name: plsB

Synonym: VV1_1165

Alternate gene names: 27364581

Gene position: 1159675-1157246 (Counterclockwise)

Preceding gene: 27364584

Following gene: 27364578

Centisome position: 35.34

GC content: 50.16

Gene sequence:

>2430_bases
ATGTCTTCAGGACAATCATTTTCACATTCATTACTGAAGCTACCGCTGTCGGCGTTGGTGAAAGGCACTGCGATTCCTTC
TAATCCAATCGACGATCACCACATCGATATTAATAAGCCCATTGTTTATGCCCTGCCTTTTCGCTCGGCCGTGGACCTAT
TAACGCTCCAAAAACACGCACTCGAGTTAGGATTACCCGATCCACTTTCTCCGCTTGAGATCCATGGCAAATCACTCAAA
CGTTATGTGTTTATCGCCTCTCGACCAACGTTGGTTCAAAGCGATAACGATGTACCAAGTGATTCGATTGCCCTTTTCTC
CGATTTACTGGCGCTCCACGCGGAAGACAGCGAACTGGATGTTCAGGTAATTCCTGCCACCGTATTGTGGGGACGTAAGC
CGGGTAAAGAAGGCAACAATAAGCCTTACCTACAAGCGATGAATGGCCTGCAAAAAGCCAAAGCAGTGATCACCGCCGGT
CGTGATTGCCTTGTGCGCTTTAGCCCTGTGGTCTCACTGCGTTACATGGCACAATCACACGGTACAGATAGCTCGATTGC
CCACAAGTTGGCACGCGTAGCTCGCATTCACTTCTCACGTCAAAAACTGGCGGCTTCTGGCCCAGACTTGCCTTCGCGCC
AAGTGCTGTTTGCTCGTTTGATGAAATCGCCAGCCATTGAGCAAGCGATTGAGGAAGAAGCCAAAAACAAAAACATCTCG
ATGGAGAAAGCGCGTAAAGAAGCGCAAGACATCATGGATGAGATTGCAGCAGACTTCTCCTACTCCTTGGTGAAACAAGG
CGATCGCTTGCTGGGATGGCTTTGGAACAAGCTCTATCAGGGGCTTAACATCAACAACGCCGCGACGGTGCGCCGTCTAG
CGCAAGATGGTCATGAGATTGTGTATGTGCCTTGCCATCGCAGCCACATGGACTACCTGCTGCTCTCTTACGTGCTTTAT
CACGAAGGGATGGTGCCACCGCATATTGCCGCAGGCATAAACCTCAACTTCTTCCCTGCGGGGCCGATTTTCCGCCGCGG
CGGGGCATTCTTTATTCGTCGTAGCTTCAAGGGCAATCGTCTTTATTCGACCATTTTCCGCGAATATTTGGCAGAGCTCT
TTGCCAAAGGCTACTCGGTAGAATACTTCTCGGAAGGTGGCCGCTCGCGCACGGGTCGTTTGCTGCCAGCTAAAACCGGC
ATGTTGGCGATGACCATTCAAGCCATGCTGCGTGGGCTAAATCGCCCAGTCACCTTAGTGCCGGTTTACATTGGCTACGA
GCACGTGATGGAAGTAGCAACCTACGCCAAAGAGTTGCGTGGTAAACGTAAAGAGAAAGAAAACGCCGGTCTTGTGCTGC
GCACACTGCGCAAACTGCGTAACTTTGGCTTGGGTTATGTCAACTTTGGTGAGCCTATCCCGCTCAACCAATACCTCAAC
GAGCATGCCCCTGAGTGGACCAAAGACATCGACCCAATGGGCGCGAGCCGACCTCAATGGATCAATCCTGTGGTGAACCA
GTTGGCGAATAAGATGATGACGCACATTAACGACGCCGCAGCAGCGAACGCATTGACCTTGTGTGCCACCGCATTACTGG
CATCACGTCAGCGGGCGCTTTCTAAAGATTCCCTAATCCATCAGATTGAGTGCTACTTACAGCTCTTGAAGAATGTGCCG
TACTCGAAGACCTATACCGTGCCTTCTGAAAGCGCCGAAGCTCTGGTAGAACACGCGATTTCATTGGATAAGTTTGTCAT
CGAAACCGACACCATGGGCGACATCATTTCGCTCGATCGTAATCAGTCGATCTTGATGACCTACTACCGCAACAACATCA
TCCACTTGTTCGCGCTACCATCGCTGATTGCACAGATGATCATTCGTCAGGAAAATCTAACCGTGAGCCAAATCCAGCAG
CAAGTGGCGGAGATTTACCCATTCCTGAAAGCGGAACTGTTCTTGAGCCACAAAGAAGAAGAGCTCGATGAACTGGTTGT
GAAAGTGCTAAACGAGTTGGTATCGCAGGATCTGATCAGCCTGAAAGAAGATAAGGTTGCGAAAAATCAAGCGAATACCC
TCACGCTTGTTTTGCTCGGTCGTACCATCTCTGAAACGCTTCAGCGTTACTCGATTGCCTTCAATCTGCTGGTGTCCAAT
CCTGAATTGGCTAAAGCGGATTTGGAACAAAAGAGCCAAGACATCGCTCAGCGTTTGACTCGCTTACACGGTATCAATGC
GCCGGAGTACTTCGACAAAGGTGTGTTCGCCTCTCTGTTTAGCACGTTAAAACAGCAAGGCTACCTTGATAGTGACGGCA
ACTGCGACGGTGAGAAAACCGCACAATTTGCTACTTTGCTCTACGCCTTGCTCTACCCAGAAGTGAAGCTCACGATTGAA
GAGAGTGTCTTCCAACTCAAATCGGCGTAA

Upstream 100 bases:

>100_bases
GCTTAAAAATTATCGCTCAGATTTATTCCTATTACTGGAATCATTCAGTAACAGAAATAAGTTTGGGTATGTTTGAATTC
TATTGAGGCTGTGAACCTAT

Downstream 100 bases:

>100_bases
GCCAGCATTAAATACAACAAAGGCATCCCGTGGATGCCTTTGTTTTTTTGTTTTGCTGTGCAACCAGCATCAAGCAGACT
CAGTGCGGTCTACCAAAACG

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 809; Mature: 808

Protein sequence:

>809_residues
MSSGQSFSHSLLKLPLSALVKGTAIPSNPIDDHHIDINKPIVYALPFRSAVDLLTLQKHALELGLPDPLSPLEIHGKSLK
RYVFIASRPTLVQSDNDVPSDSIALFSDLLALHAEDSELDVQVIPATVLWGRKPGKEGNNKPYLQAMNGLQKAKAVITAG
RDCLVRFSPVVSLRYMAQSHGTDSSIAHKLARVARIHFSRQKLAASGPDLPSRQVLFARLMKSPAIEQAIEEEAKNKNIS
MEKARKEAQDIMDEIAADFSYSLVKQGDRLLGWLWNKLYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSYVLY
HEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTG
MLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENAGLVLRTLRKLRNFGLGYVNFGEPIPLNQYLN
EHAPEWTKDIDPMGASRPQWINPVVNQLANKMMTHINDAAAANALTLCATALLASRQRALSKDSLIHQIECYLQLLKNVP
YSKTYTVPSESAEALVEHAISLDKFVIETDTMGDIISLDRNQSILMTYYRNNIIHLFALPSLIAQMIIRQENLTVSQIQQ
QVAEIYPFLKAELFLSHKEEELDELVVKVLNELVSQDLISLKEDKVAKNQANTLTLVLLGRTISETLQRYSIAFNLLVSN
PELAKADLEQKSQDIAQRLTRLHGINAPEYFDKGVFASLFSTLKQQGYLDSDGNCDGEKTAQFATLLYALLYPEVKLTIE
ESVFQLKSA

Sequences:

>Translated_809_residues
MSSGQSFSHSLLKLPLSALVKGTAIPSNPIDDHHIDINKPIVYALPFRSAVDLLTLQKHALELGLPDPLSPLEIHGKSLK
RYVFIASRPTLVQSDNDVPSDSIALFSDLLALHAEDSELDVQVIPATVLWGRKPGKEGNNKPYLQAMNGLQKAKAVITAG
RDCLVRFSPVVSLRYMAQSHGTDSSIAHKLARVARIHFSRQKLAASGPDLPSRQVLFARLMKSPAIEQAIEEEAKNKNIS
MEKARKEAQDIMDEIAADFSYSLVKQGDRLLGWLWNKLYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSYVLY
HEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTG
MLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENAGLVLRTLRKLRNFGLGYVNFGEPIPLNQYLN
EHAPEWTKDIDPMGASRPQWINPVVNQLANKMMTHINDAAAANALTLCATALLASRQRALSKDSLIHQIECYLQLLKNVP
YSKTYTVPSESAEALVEHAISLDKFVIETDTMGDIISLDRNQSILMTYYRNNIIHLFALPSLIAQMIIRQENLTVSQIQQ
QVAEIYPFLKAELFLSHKEEELDELVVKVLNELVSQDLISLKEDKVAKNQANTLTLVLLGRTISETLQRYSIAFNLLVSN
PELAKADLEQKSQDIAQRLTRLHGINAPEYFDKGVFASLFSTLKQQGYLDSDGNCDGEKTAQFATLLYALLYPEVKLTIE
ESVFQLKSA
>Mature_808_residues
SSGQSFSHSLLKLPLSALVKGTAIPSNPIDDHHIDINKPIVYALPFRSAVDLLTLQKHALELGLPDPLSPLEIHGKSLKR
YVFIASRPTLVQSDNDVPSDSIALFSDLLALHAEDSELDVQVIPATVLWGRKPGKEGNNKPYLQAMNGLQKAKAVITAGR
DCLVRFSPVVSLRYMAQSHGTDSSIAHKLARVARIHFSRQKLAASGPDLPSRQVLFARLMKSPAIEQAIEEEAKNKNISM
EKARKEAQDIMDEIAADFSYSLVKQGDRLLGWLWNKLYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSYVLYH
EGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGM
LAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENAGLVLRTLRKLRNFGLGYVNFGEPIPLNQYLNE
HAPEWTKDIDPMGASRPQWINPVVNQLANKMMTHINDAAAANALTLCATALLASRQRALSKDSLIHQIECYLQLLKNVPY
SKTYTVPSESAEALVEHAISLDKFVIETDTMGDIISLDRNQSILMTYYRNNIIHLFALPSLIAQMIIRQENLTVSQIQQQ
VAEIYPFLKAELFLSHKEEELDELVVKVLNELVSQDLISLKEDKVAKNQANTLTLVLLGRTISETLQRYSIAFNLLVSNP
ELAKADLEQKSQDIAQRLTRLHGINAPEYFDKGVFASLFSTLKQQGYLDSDGNCDGEKTAQFATLLYALLYPEVKLTIEE
SVFQLKSA

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=427, Percent_Identity=27.4004683840749, Blast_Score=157, Evalue=4e-38,
Organism=Homo sapiens, GI190358539, Length=435, Percent_Identity=24.1379310344828, Blast_Score=123, Evalue=6e-28,
Organism=Escherichia coli, GI87082362, Length=804, Percent_Identity=56.9651741293532, Blast_Score=935, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=265, Percent_Identity=33.9622641509434, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71988723, Length=475, Percent_Identity=26.3157894736842, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71988728, Length=415, Percent_Identity=27.710843373494, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17864692, Length=309, Percent_Identity=31.7152103559871, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21357731, Length=193, Percent_Identity=33.160621761658, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650754, Length=193, Percent_Identity=33.160621761658, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650752, Length=193, Percent_Identity=33.160621761658, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_VIBVU (Q8DD48)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760109.1
- ProteinModelPortal:   Q8DD48
- GeneID:   1178091
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1165
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- BioCyc:   VVUL216895:VV1_1165-MONOMER
- BRENDA:   2.3.1.15
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 90598; Mature: 90466

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGQSFSHSLLKLPLSALVKGTAIPSNPIDDHHIDINKPIVYALPFRSAVDLLTLQKHA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHH
LELGLPDPLSPLEIHGKSLKRYVFIASRPTLVQSDNDVPSDSIALFSDLLALHAEDSELD
HHCCCCCCCCHHHHCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCE
VQVIPATVLWGRKPGKEGNNKPYLQAMNGLQKAKAVITAGRDCLVRFSPVVSLRYMAQSH
EEEECHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GTDSSIAHKLARVARIHFSRQKLAASGPDLPSRQVLFARLMKSPAIEQAIEEEAKNKNIS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
MEKARKEAQDIMDEIAADFSYSLVKQGDRLLGWLWNKLYQGLNINNAATVRRLAQDGHEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
PVYIGYEHVMEVATYAKELRGKRKEKENAGLVLRTLRKLRNFGLGYVNFGEPIPLNQYLN
EEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
EHAPEWTKDIDPMGASRPQWINPVVNQLANKMMTHINDAAAANALTLCATALLASRQRAL
HCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKDSLIHQIECYLQLLKNVPYSKTYTVPSESAEALVEHAISLDKFVIETDTMGDIISLDR
HHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHEEECCCCCCHHCCCC
NQSILMTYYRNNIIHLFALPSLIAQMIIRQENLTVSQIQQQVAEIYPFLKAELFLSHKEE
CCCEEEEEHHCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ELDELVVKVLNELVSQDLISLKEDKVAKNQANTLTLVLLGRTISETLQRYSIAFNLLVSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHEEEECC
PELAKADLEQKSQDIAQRLTRLHGINAPEYFDKGVFASLFSTLKQQGYLDSDGNCDGEKT
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AQFATLLYALLYPEVKLTIEESVFQLKSA
HHHHHHHHHHHCCCCEEEHHHHHHHHCCC
>Mature Secondary Structure 
SSGQSFSHSLLKLPLSALVKGTAIPSNPIDDHHIDINKPIVYALPFRSAVDLLTLQKHA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHH
LELGLPDPLSPLEIHGKSLKRYVFIASRPTLVQSDNDVPSDSIALFSDLLALHAEDSELD
HHCCCCCCCCHHHHCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCE
VQVIPATVLWGRKPGKEGNNKPYLQAMNGLQKAKAVITAGRDCLVRFSPVVSLRYMAQSH
EEEECHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GTDSSIAHKLARVARIHFSRQKLAASGPDLPSRQVLFARLMKSPAIEQAIEEEAKNKNIS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
MEKARKEAQDIMDEIAADFSYSLVKQGDRLLGWLWNKLYQGLNINNAATVRRLAQDGHEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
PVYIGYEHVMEVATYAKELRGKRKEKENAGLVLRTLRKLRNFGLGYVNFGEPIPLNQYLN
EEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
EHAPEWTKDIDPMGASRPQWINPVVNQLANKMMTHINDAAAANALTLCATALLASRQRAL
HCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKDSLIHQIECYLQLLKNVPYSKTYTVPSESAEALVEHAISLDKFVIETDTMGDIISLDR
HHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHEEECCCCCCHHCCCC
NQSILMTYYRNNIIHLFALPSLIAQMIIRQENLTVSQIQQQVAEIYPFLKAELFLSHKEE
CCCEEEEEHHCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ELDELVVKVLNELVSQDLISLKEDKVAKNQANTLTLVLLGRTISETLQRYSIAFNLLVSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHEEEECC
PELAKADLEQKSQDIAQRLTRLHGINAPEYFDKGVFASLFSTLKQQGYLDSDGNCDGEKT
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AQFATLLYALLYPEVKLTIEESVFQLKSA
HHHHHHHHHHHCCCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA