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The map label for this gene is thiC

Identifier: 27364403

GI number: 27364403

Start: 969024

End: 970967

Strand: Reverse

Name: thiC

Synonym: VV1_0964

Alternate gene names: 27364403

Gene position: 970967-969024 (Counterclockwise)

Preceding gene: 27364404

Following gene: 27364402

Centisome position: 29.59

GC content: 50.21

Gene sequence:

>1944_bases
ATGTCGAATCGCAAGCAAGCGAGACTGGAAGCTAAACAATTCATCGATACACTCTCTGTGCAGCCGTATCCCAATTCCAC
CAAAGTGTATGTCGAGGGCTCTCGCTCAGACATTAGAGTGCCAATGCGCGAAATCTCCTTGGCAGACAGCTTAGTTGGCG
GGACCAAAGAGGCACCGATCTTTCAGCCAAACGAAGCGGTAAGAGTGTATGACACTTCGGGTGTCTACACTGATCCGACC
CATCAAATCGATCTCTATAATGGCCTTCCTAAGTTAAGAGAAGAGTGGATTGCAGAGCGTGCTGATACCGAAGTGCTTGA
TGATGTCAGCTCGGTCTACACGAAAGAGCGCTTAGAAGATGAAACGCTGGACGAGTTGAGATATGGCAATCTGCCTCGCA
TCCGTCGCGCCAAAGCTGGGTATTGTGTCACTCAGTTGCATTATGCGCGTAAGGGAATCATCACCCCGGAGATGGAATAC
ATCGCTTTGCGCGAGAACATGGGGCGCGCCAAATATCAAGATGAAGTTTTGACTCAGCAGCATGCAGGGCAAAGCTTTGG
TGCGAATCTACCTAAGGAAATTACCCCAGAATTTGTGCGTCGTGAAGTGGCGGAAGGTCGCGCGATCATTCCTTCCAACA
TTAATCACCCAGAATCCGAGCCGATGATCATCGGCCGCAACTTCCTTGTGAAAGTTAACGCCAACATTGGTAACTCATCG
GTGACCTCGTCGATTGAAGAAGAAGTGGAGAAGTTGGTGTGGTCGACTCGTTGGGGCGGTGATACGGTGATGGATCTGTC
GACGGGGAGAAACATTCACGAAACTCGCGAATGGATTCTACGCAACAGCCCAGTACCGATTGGAACCGTGCCTATGTATC
AAGCACTAGAGAAGGTCAATGGCGTTGCTGAGAACCTAACGTGGGAAGTGATGCGTGATACGTTGATCGAGCAGGCTGAG
CAGGGGGTGGACTACTTCACGATTCATGCTGGTTTGCTGCTGCGCTATATCCCAATGACGGCGAAACGCGTGACGGGCAT
TGTCTCGCGTGGGGGTTCGATCATCGCTAAGTGGTGTTTGGCACATCATCAAGAGAGTTTCCTCTATACCCATTTCCGCG
AGATCTGTGAGATTTGTGCGAAGTACGATGTAGCGCTCTCATTAGGTGATGGTTTGCGTCCTGGCTCGATTGCCGATGCC
AATGATGAAGCGCAGTTTGCCGAGCTCAGAACATTGGGTGAACTGACCAAAATCGCGTGGGAATACGATGTCCAAGTGAT
TATTGAAGGTCCTGGGCATGTACCGATGCACATGATTAAAGAGAATATGGATGAGCAGCTCAAGCATTGTCATGAGGCAC
CATTCTATACCTTGGGTCCACTGACCACCGACATTGCCCCTGGCTATGACCACATTACTTCTGGGATTGGTGCAGCCATG
ATCGGTTGGTATGGCTGTGCCATGCTTTGCTACGTAACGCCAAAAGAACACTTAGGTCTACCCAATAAAGAAGACGTGAA
AACCGGCTTGATAACCTACAAACTGGCTGCACACGCGGCGGATTTAGCTAAAGGGCATCCGGGTGCACAAGTACGTGACA
ATGCCTTGTCAAAAGCACGATTTGAGTTCCGCTGGCAAGATCAGTTCAATCTCTCTCTTGACCCCGATACGGCGCGTGCA
TTCCACGATGAAACCTTACCTCAGGAATCAGGCAAGGTGGCACACTTTTGTTCGATGTGTGGTCCAAAATTCTGCTCGAT
GAAGATTTCGCAAGAGGTTCGTGAGTACGCTAAAGGGCAACAAGGTGAGGCAGGACAGGCGATAGAAGTGAAGCTGTTGG
ATGACCCATTGGAAGGAATGAAGCAAAAGTCCGCGGAATTCAAAGCGTCGGGTTCGGAGTTGTACCATCCTGCAGTGAGT
CACGAAGAAGTGGCAGAGGGTTAA

Upstream 100 bases:

>100_bases
AAACTCTAAATTGTGTGTTCAACGCCTCTTGTCACAACTTTCCGCCAAGCATTTTTGTCCAAAACCTTTTTATCCAGCAT
AACAAGAAAGGAAAAATGCT

Downstream 100 bases:

>100_bases
GTATGAGACTGCTGATTCCTTCTGCCATTATTGAGCTGACGGGAGAAATTCAGCAGTGTTTGCTCTTGGCAAAGCAGCAA
GGTTTTGCCATTGACCATAT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MSNRKQARLEAKQFIDTLSVQPYPNSTKVYVEGSRSDIRVPMREISLADSLVGGTKEAPIFQPNEAVRVYDTSGVYTDPT
HQIDLYNGLPKLREEWIAERADTEVLDDVSSVYTKERLEDETLDELRYGNLPRIRRAKAGYCVTQLHYARKGIITPEMEY
IALRENMGRAKYQDEVLTQQHAGQSFGANLPKEITPEFVRREVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
VTSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLTWEVMRDTLIEQAE
QGVDYFTIHAGLLLRYIPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADA
NDEAQFAELRTLGELTKIAWEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWQDQFNLSLDPDTARA
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKGQQGEAGQAIEVKLLDDPLEGMKQKSAEFKASGSELYHPAVS
HEEVAEG

Sequences:

>Translated_647_residues
MSNRKQARLEAKQFIDTLSVQPYPNSTKVYVEGSRSDIRVPMREISLADSLVGGTKEAPIFQPNEAVRVYDTSGVYTDPT
HQIDLYNGLPKLREEWIAERADTEVLDDVSSVYTKERLEDETLDELRYGNLPRIRRAKAGYCVTQLHYARKGIITPEMEY
IALRENMGRAKYQDEVLTQQHAGQSFGANLPKEITPEFVRREVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
VTSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLTWEVMRDTLIEQAE
QGVDYFTIHAGLLLRYIPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADA
NDEAQFAELRTLGELTKIAWEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWQDQFNLSLDPDTARA
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKGQQGEAGQAIEVKLLDDPLEGMKQKSAEFKASGSELYHPAVS
HEEVAEG
>Mature_646_residues
SNRKQARLEAKQFIDTLSVQPYPNSTKVYVEGSRSDIRVPMREISLADSLVGGTKEAPIFQPNEAVRVYDTSGVYTDPTH
QIDLYNGLPKLREEWIAERADTEVLDDVSSVYTKERLEDETLDELRYGNLPRIRRAKAGYCVTQLHYARKGIITPEMEYI
ALRENMGRAKYQDEVLTQQHAGQSFGANLPKEITPEFVRREVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSSV
TSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLTWEVMRDTLIEQAEQ
GVDYFTIHAGLLLRYIPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADAN
DEAQFAELRTLGELTKIAWEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMI
GWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWQDQFNLSLDPDTARAF
HDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKGQQGEAGQAIEVKLLDDPLEGMKQKSAEFKASGSELYHPAVSH
EEVAEG

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=633, Percent_Identity=75.8293838862559, Blast_Score=1011, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_VIBVU (Q8DDL4)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759931.1
- GeneID:   1177901
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0964
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   VVUL216895:VV1_0964-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 72428; Mature: 72297

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: BINDING 235-235 BINDING 264-264 BINDING 293-293 BINDING 329-329 BINDING 429-429 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRKQARLEAKQFIDTLSVQPYPNSTKVYVEGSRSDIRVPMREISLADSLVGGTKEAPI
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
FQPNEAVRVYDTSGVYTDPTHQIDLYNGLPKLREEWIAERADTEVLDDVSSVYTKERLED
CCCCCCEEEEECCCCCCCCCCEEEHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCH
ETLDELRYGNLPRIRRAKAGYCVTQLHYARKGIITPEMEYIALRENMGRAKYQDEVLTQQ
HHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
HAGQSFGANLPKEITPEFVRREVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
HCCCCCCCCCCHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCEEEEEEECCCCCHH
VTSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVN
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAENLTWEVMRDTLIEQAEQGVDYFTIHAGLLLRYIPMTAKRVTGIVSRGGSIIAKWCL
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADANDEAQFAELRTLGELTKIAW
HHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHE
EYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
EECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQVRDNALSKAR
HHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHE
FEFRWQDQFNLSLDPDTARAFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKGQ
EEEEECCCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCC
QGEAGQAIEVKLLDDPLEGMKQKSAEFKASGSELYHPAVSHEEVAEG
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHCCCCCCCHHHCCC
>Mature Secondary Structure 
SNRKQARLEAKQFIDTLSVQPYPNSTKVYVEGSRSDIRVPMREISLADSLVGGTKEAPI
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
FQPNEAVRVYDTSGVYTDPTHQIDLYNGLPKLREEWIAERADTEVLDDVSSVYTKERLED
CCCCCCEEEEECCCCCCCCCCEEEHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCH
ETLDELRYGNLPRIRRAKAGYCVTQLHYARKGIITPEMEYIALRENMGRAKYQDEVLTQQ
HHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
HAGQSFGANLPKEITPEFVRREVAEGRAIIPSNINHPESEPMIIGRNFLVKVNANIGNSS
HCCCCCCCCCCHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCEEEEEEECCCCCHH
VTSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVN
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAENLTWEVMRDTLIEQAEQGVDYFTIHAGLLLRYIPMTAKRVTGIVSRGGSIIAKWCL
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AHHQESFLYTHFREICEICAKYDVALSLGDGLRPGSIADANDEAQFAELRTLGELTKIAW
HHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHE
EYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
EECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IGWYGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQVRDNALSKAR
HHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHE
FEFRWQDQFNLSLDPDTARAFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKGQ
EEEEECCCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCC
QGEAGQAIEVKLLDDPLEGMKQKSAEFKASGSELYHPAVSHEEVAEG
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA