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The map label for this gene is thiF [H]

Identifier: 27364401

GI number: 27364401

Start: 966861

End: 967697

Strand: Reverse

Name: thiF [H]

Synonym: VV1_0962

Alternate gene names: 27364401

Gene position: 967697-966861 (Counterclockwise)

Preceding gene: 27364402

Following gene: 27364400

Centisome position: 29.49

GC content: 48.98

Gene sequence:

>837_bases
ATGTTGGTTGATCGGCTCTTTACTCGTTATCAAAGACAAATTGCATTAACGGAATTTGGTGAGTCAGGGCAAGCCAACTT
ACTTCAAGCACATGTGCTGATCATCGGTTGCGGTGGTCTTGGTAACGCAGCGGCGCTTTATTTGGCTGCTGCGGGCGTTG
GCCATTTAGTGTTGGTGGATGATGATGTGGTTGAAGAGAGTAACTTGCAGCGTCAAATTGCGTTTCGTCAGCAACATCTC
GCCAGCCCCAAAGTGGATGCACTCGCAGAGCAATTGAAGCAGCTCAATGCAGAATTGCGCGTTCGCACCATTAGCCACCG
TTTGGATGAAGAACGTCTCAACTTGGAAGTGATGTTGGCGGACATTGTGCTCGATTGTACGGACAACTTTCCATCGCGCC
AACTGATCAACCAAGCCTGCCTGAATCAACGTACCCCATTAGTGTCGGCCTCGGCAATTGGCTGGAAAGGGCAATTCATT
GCGTTTGATTTTAGCTCATACCACTCAATCCAATCCCAGCAAGATACCTCGTCGGCCGCGAGTTCAGTAAACCCTCCGTG
CTACCACTGCCTATTTCCATTTGCAGAAAATGCCATGCAGACCAAGTGCAGTGATGCTGGGGTGATTGGCCCTGTCGTGG
GGCTAATGGGAAATTATCAGGCAATCGCAACGATTCAAAAGCTGGCAACGGGCGCGTTTCAAACCACTTGCCATCAGTTG
CATCTGTTTGATGGGCTCAAGTTAAGTTGGCAGCATTTGGCAGTAGTGAAAGATCCAAGTTGCCGCGTTTGTCAGATTCA
GAAACAGGAATCAAGCAATGAACACCATAGCAATTAG

Upstream 100 bases:

>100_bases
GTAATAGAGTACTTCAAGCAAGTGATGGCACCTAACACAACACTGCTGCGTTTAGACTCAGTATCCACACATAGTCTTGA
GCCAACAAGTGAGGCCAATC

Downstream 100 bases:

>100_bases
TATCAACGGCGAAAGCTACGAGGTGCCAACCAATGCGACTTTGGCATCCATTATTGAGCATCTTTCACTACCTAACTTGG
GTTGTGTGTTTGCAATCAAT

Product: sulfur carrier protein adenylyltransferase ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MLVDRLFTRYQRQIALTEFGESGQANLLQAHVLIIGCGGLGNAAALYLAAAGVGHLVLVDDDVVEESNLQRQIAFRQQHL
ASPKVDALAEQLKQLNAELRVRTISHRLDEERLNLEVMLADIVLDCTDNFPSRQLINQACLNQRTPLVSASAIGWKGQFI
AFDFSSYHSIQSQQDTSSAASSVNPPCYHCLFPFAENAMQTKCSDAGVIGPVVGLMGNYQAIATIQKLATGAFQTTCHQL
HLFDGLKLSWQHLAVVKDPSCRVCQIQKQESSNEHHSN

Sequences:

>Translated_278_residues
MLVDRLFTRYQRQIALTEFGESGQANLLQAHVLIIGCGGLGNAAALYLAAAGVGHLVLVDDDVVEESNLQRQIAFRQQHL
ASPKVDALAEQLKQLNAELRVRTISHRLDEERLNLEVMLADIVLDCTDNFPSRQLINQACLNQRTPLVSASAIGWKGQFI
AFDFSSYHSIQSQQDTSSAASSVNPPCYHCLFPFAENAMQTKCSDAGVIGPVVGLMGNYQAIATIQKLATGAFQTTCHQL
HLFDGLKLSWQHLAVVKDPSCRVCQIQKQESSNEHHSN
>Mature_278_residues
MLVDRLFTRYQRQIALTEFGESGQANLLQAHVLIIGCGGLGNAAALYLAAAGVGHLVLVDDDVVEESNLQRQIAFRQQHL
ASPKVDALAEQLKQLNAELRVRTISHRLDEERLNLEVMLADIVLDCTDNFPSRQLINQACLNQRTPLVSASAIGWKGQFI
AFDFSSYHSIQSQQDTSSAASSVNPPCYHCLFPFAENAMQTKCSDAGVIGPVVGLMGNYQAIATIQKLATGAFQTTCHQL
HLFDGLKLSWQHLAVVKDPSCRVCQIQKQESSNEHHSN

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=240, Percent_Identity=40, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI23510338, Length=181, Percent_Identity=29.8342541436464, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI23510340, Length=181, Percent_Identity=29.8342541436464, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI38045948, Length=164, Percent_Identity=31.7073170731707, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=137, Percent_Identity=27.007299270073, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI87082356, Length=265, Percent_Identity=43.7735849056604, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787048, Length=264, Percent_Identity=36.7424242424242, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789177, Length=137, Percent_Identity=27.7372262773723, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=258, Percent_Identity=38.3720930232558, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI193203301, Length=134, Percent_Identity=31.3432835820896, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI86565433, Length=188, Percent_Identity=26.063829787234, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=189, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=230, Percent_Identity=34.7826086956522, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322639, Length=191, Percent_Identity=29.3193717277487, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582879, Length=263, Percent_Identity=36.8821292775665, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28573937, Length=182, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24660640, Length=141, Percent_Identity=26.241134751773, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 30556; Mature: 30556

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVDRLFTRYQRQIALTEFGESGQANLLQAHVLIIGCGGLGNAAALYLAAAGVGHLVLVD
CCHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEC
DDVVEESNLQRQIAFRQQHLASPKVDALAEQLKQLNAELRVRTISHRLDEERLNLEVMLA
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEEEE
DIVLDCTDNFPSRQLINQACLNQRTPLVSASAIGWKGQFIAFDFSSYHSIQSQQDTSSAA
HHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECHHHHHHHCHHHHHHHH
SSVNPPCYHCLFPFAENAMQTKCSDAGVIGPVVGLMGNYQAIATIQKLATGAFQTTCHQL
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
HLFDGLKLSWQHLAVVKDPSCRVCQIQKQESSNEHHSN
HHCCCCEECEEEEEEEECCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLVDRLFTRYQRQIALTEFGESGQANLLQAHVLIIGCGGLGNAAALYLAAAGVGHLVLVD
CCHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEC
DDVVEESNLQRQIAFRQQHLASPKVDALAEQLKQLNAELRVRTISHRLDEERLNLEVMLA
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEEEE
DIVLDCTDNFPSRQLINQACLNQRTPLVSASAIGWKGQFIAFDFSSYHSIQSQQDTSSAA
HHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECHHHHHHHCHHHHHHHH
SSVNPPCYHCLFPFAENAMQTKCSDAGVIGPVVGLMGNYQAIATIQKLATGAFQTTCHQL
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
HLFDGLKLSWQHLAVVKDPSCRVCQIQKQESSNEHHSN
HHCCCCEECEEEEEEEECCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]