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The map label for this gene is thiG

Identifier: 27364399

GI number: 27364399

Start: 965893

End: 966666

Strand: Reverse

Name: thiG

Synonym: VV1_0960

Alternate gene names: 27364399

Gene position: 966666-965893 (Counterclockwise)

Preceding gene: 27364400

Following gene: 27364398

Centisome position: 29.45

GC content: 51.81

Gene sequence:

>774_bases
ATGCTCAAAATCGCAGACAAACAATTTCATTCTCGCTTGTTCACGGGCACCGGTAAATTTGCCAACAGCCAATTGATGTC
ACAAGCCATCGAACAATCAGGTTCACAGCTCGCCACTATGGCGCTGAAAAGGGTGGATATCCACAATCAGCAAGATGACA
TCTTGTCACCACTGTTGTCAGCGGGTGTTAATTTGCTACCTAATACCTCTGGTGCCAAAAACGCCAAAGACGCCATTTTT
GCCGCGCATTTAGCGCGTGAAGCATTGGGCACTAATTGGCTGAAATTGGAAATTCATCCCGATCCGAAATACCTGATGCC
CGATCCGATTGAAACATTGGCGGCAGCAGAGCAGCTAGTCAAAGACGGCTTTGTGGTGTTGCCTTATTGCCATGCAGACC
CGGTGTTGTGTAAGCGATTGGAAGAAGTAGGTTGCGCTGCCGTCATGCCTTTGGGAGCGCCAATTGGCTCAAACAAAGGC
TTGGTTTCGCAAGACTTCTTACAAATCATCATCGACCAAGCGAAGGTTCCTGTTGTGGTGGATGCGGGGATTGGCGCACC
GTCTCATGCTGCCTATGCGATGGAACTGGGTGCTGATGCGGTGCTGGTGAATACCGCGATCGCCGCTGCACGCGATCCCA
TCGCCATGGCCCGTGCATTCAAATTGGCGGTTGAGGCAGGGCGTTTGGCGTATGAGTCGGGGCTTGCAGGCAAAGTGTCG
CATGCGGTGGCATCCAGCCCATTGACCTCTTTTCTCGATGAGTGCCTTTCTTAG

Upstream 100 bases:

>100_bases
ATCAATAATCAAGTTGTCCCACACAGCGAATGGCAAACCAAAACGCTCAGTCGTGGTGATGCTATCTCACTATTTCAGGC
GATTGCTGGAGGATAAGCAC

Downstream 100 bases:

>100_bases
CAAAAGAAAGGGCCGTCTGTTGACCGGAGCAACACCATGAGTTTCGTCGAACATTTTAATCAGCTCAATTGGGACGATAT
TCGATTATCGATCTACTCTA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLKIADKQFHSRLFTGTGKFANSQLMSQAIEQSGSQLATMALKRVDIHNQQDDILSPLLSAGVNLLPNTSGAKNAKDAIF
AAHLAREALGTNWLKLEIHPDPKYLMPDPIETLAAAEQLVKDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKG
LVSQDFLQIIIDQAKVPVVVDAGIGAPSHAAYAMELGADAVLVNTAIAAARDPIAMARAFKLAVEAGRLAYESGLAGKVS
HAVASSPLTSFLDECLS

Sequences:

>Translated_257_residues
MLKIADKQFHSRLFTGTGKFANSQLMSQAIEQSGSQLATMALKRVDIHNQQDDILSPLLSAGVNLLPNTSGAKNAKDAIF
AAHLAREALGTNWLKLEIHPDPKYLMPDPIETLAAAEQLVKDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKG
LVSQDFLQIIIDQAKVPVVVDAGIGAPSHAAYAMELGADAVLVNTAIAAARDPIAMARAFKLAVEAGRLAYESGLAGKVS
HAVASSPLTSFLDECLS
>Mature_257_residues
MLKIADKQFHSRLFTGTGKFANSQLMSQAIEQSGSQLATMALKRVDIHNQQDDILSPLLSAGVNLLPNTSGAKNAKDAIF
AAHLAREALGTNWLKLEIHPDPKYLMPDPIETLAAAEQLVKDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKG
LVSQDFLQIIIDQAKVPVVVDAGIGAPSHAAYAMELGADAVLVNTAIAAARDPIAMARAFKLAVEAGRLAYESGLAGKVS
HAVASSPLTSFLDECLS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=73.5177865612648, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_VIBVU (Q8DDL8)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759927.1
- ProteinModelPortal:   Q8DDL8
- SMR:   Q8DDL8
- GeneID:   1177897
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0960
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   VVUL216895:VV1_0960-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27092; Mature: 27092

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIADKQFHSRLFTGTGKFANSQLMSQAIEQSGSQLATMALKRVDIHNQQDDILSPLLS
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AGVNLLPNTSGAKNAKDAIFAAHLAREALGTNWLKLEIHPDPKYLMPDPIETLAAAEQLV
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
KDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKGLVSQDFLQIIIDQAKVPVVV
HCCCEEEEECCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
DAGIGAPSHAAYAMELGADAVLVNTAIAAARDPIAMARAFKLAVEAGRLAYESGLAGKVS
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HAVASSPLTSFLDECLS
HHHHHCHHHHHHHHHCC
>Mature Secondary Structure
MLKIADKQFHSRLFTGTGKFANSQLMSQAIEQSGSQLATMALKRVDIHNQQDDILSPLLS
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AGVNLLPNTSGAKNAKDAIFAAHLAREALGTNWLKLEIHPDPKYLMPDPIETLAAAEQLV
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
KDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKGLVSQDFLQIIIDQAKVPVVV
HCCCEEEEECCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
DAGIGAPSHAAYAMELGADAVLVNTAIAAARDPIAMARAFKLAVEAGRLAYESGLAGKVS
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HAVASSPLTSFLDECLS
HHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA