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The map label for this gene is thiH [H]

Identifier: 27364398

GI number: 27364398

Start: 964732

End: 965856

Strand: Reverse

Name: thiH [H]

Synonym: VV1_0959

Alternate gene names: 27364398

Gene position: 965856-964732 (Counterclockwise)

Preceding gene: 27364399

Following gene: 27364395

Centisome position: 29.43

GC content: 48.89

Gene sequence:

>1125_bases
ATGAGTTTCGTCGAACATTTTAATCAGCTCAATTGGGACGATATTCGATTATCGATCTACTCTAAAACCGCGCTCGATGT
CGAGCGAGCGCTGAGTAAAAACAAACTCGATTTAGAAGACTTTAAAGCGCTGATTTCGCCAGCCGCGGAGCACTATCTAG
AAGCGATGGCGCAGCGTTCCTATGCGTTAACCCGTAAACGTTTTGGTAATACGTTATCGCTCTATATACCACTGTATCTT
TCCAATCTCTGTGCCAATGCGTGTACGTATTGTGGCTTCTCGATGGAGAATCGCCTAAAGCGCCGCACACTCACGGTGCA
AGAAATCTATGCAGAAATGGCGGCGATTAAGGCAATGAAGTTTGACAGCGTTTTGCTGGTGACGGGTGAGCATGAAGGCA
AGGTTGGTATGAATTATTTCCGCCAAGTGCTGCCGCAAATCAAACGCGCTTTTAACTATCTAGCGATGGAAGTGCAGCCG
CTTAGCCAAGAGCATTATCAAGAGTTGAAGCTTTTGGGTTTGGACGCTGTGATGGTTTATCAGGAAACGTATCACCCTGC
GACTTACGCACAACATCACTTGCGTGGCAACAAAACCAATTTCAACTATCGTCTCGAAACGCCTGACCGTTTGGCGCGAG
CGGGGATCGATAAAATTGGCATTGGTGCACTGATCGGCTTGGAGGAGTGGCGTACGGACTGCTTCTACGTTGCTGCGCAT
CTGGACTATTTGGAACGTACCTATTGGCAGACACGCTATTCAATTTCATTTCCTCGCTTGCGTCCTTGCGCAGGGGCGTT
GCAGCCTAAATCGGTAATGAGTGATAAGCAGCTGGTTCAGCTGATTTGTGCCTATCGTTTGTTTAATCCAGAAGTCGAGC
TATCGTTGTCTACACGCGAATCGGCACTGTTTCGCGATAACGTACTGCCACTGGGTATTACGTCGATGTCAGCGGCATCG
AAAACTCAGCCGGGCGGTTATGCGATGCCTGATGTTGAACTCGAGCAGTTTGAGATCAGTGATGAAAGGAGTGCGGCGGA
AGTGGAGCAGATGATTCGTAGTAAAGGGTTCGATCCCGTATGGCGCGATTGGCATGCCGCCTATTCTGGGGCTCGCTTCT
CTTAA

Upstream 100 bases:

>100_bases
CAAAGTGTCGCATGCGGTGGCATCCAGCCCATTGACCTCTTTTCTCGATGAGTGCCTTTCTTAGCAAAAGAAAGGGCCGT
CTGTTGACCGGAGCAACACC

Downstream 100 bases:

>100_bases
CCAAAAAAGCTAATCTTATCTTAGGCAATACAGTCGTTATCGAGCGAGTCTGAGCGCGGTTCGATAATGTCAGCAAAGAG
CGAAAGTGGCATGTGGATAT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSFVEHFNQLNWDDIRLSIYSKTALDVERALSKNKLDLEDFKALISPAAEHYLEAMAQRSYALTRKRFGNTLSLYIPLYL
SNLCANACTYCGFSMENRLKRRTLTVQEIYAEMAAIKAMKFDSVLLVTGEHEGKVGMNYFRQVLPQIKRAFNYLAMEVQP
LSQEHYQELKLLGLDAVMVYQETYHPATYAQHHLRGNKTNFNYRLETPDRLARAGIDKIGIGALIGLEEWRTDCFYVAAH
LDYLERTYWQTRYSISFPRLRPCAGALQPKSVMSDKQLVQLICAYRLFNPEVELSLSTRESALFRDNVLPLGITSMSAAS
KTQPGGYAMPDVELEQFEISDERSAAEVEQMIRSKGFDPVWRDWHAAYSGARFS

Sequences:

>Translated_374_residues
MSFVEHFNQLNWDDIRLSIYSKTALDVERALSKNKLDLEDFKALISPAAEHYLEAMAQRSYALTRKRFGNTLSLYIPLYL
SNLCANACTYCGFSMENRLKRRTLTVQEIYAEMAAIKAMKFDSVLLVTGEHEGKVGMNYFRQVLPQIKRAFNYLAMEVQP
LSQEHYQELKLLGLDAVMVYQETYHPATYAQHHLRGNKTNFNYRLETPDRLARAGIDKIGIGALIGLEEWRTDCFYVAAH
LDYLERTYWQTRYSISFPRLRPCAGALQPKSVMSDKQLVQLICAYRLFNPEVELSLSTRESALFRDNVLPLGITSMSAAS
KTQPGGYAMPDVELEQFEISDERSAAEVEQMIRSKGFDPVWRDWHAAYSGARFS
>Mature_373_residues
SFVEHFNQLNWDDIRLSIYSKTALDVERALSKNKLDLEDFKALISPAAEHYLEAMAQRSYALTRKRFGNTLSLYIPLYLS
NLCANACTYCGFSMENRLKRRTLTVQEIYAEMAAIKAMKFDSVLLVTGEHEGKVGMNYFRQVLPQIKRAFNYLAMEVQPL
SQEHYQELKLLGLDAVMVYQETYHPATYAQHHLRGNKTNFNYRLETPDRLARAGIDKIGIGALIGLEEWRTDCFYVAAHL
DYLERTYWQTRYSISFPRLRPCAGALQPKSVMSDKQLVQLICAYRLFNPEVELSLSTRESALFRDNVLPLGITSMSAASK
TQPGGYAMPDVELEQFEISDERSAAEVEQMIRSKGFDPVWRDWHAAYSGARFS

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=61.4754098360656, Blast_Score=480, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42949; Mature: 42817

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVEHFNQLNWDDIRLSIYSKTALDVERALSKNKLDLEDFKALISPAAEHYLEAMAQRS
CCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YALTRKRFGNTLSLYIPLYLSNLCANACTYCGFSMENRLKRRTLTVQEIYAEMAAIKAMK
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FDSVLLVTGEHEGKVGMNYFRQVLPQIKRAFNYLAMEVQPLSQEHYQELKLLGLDAVMVY
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCHHHEEE
QETYHPATYAQHHLRGNKTNFNYRLETPDRLARAGIDKIGIGALIGLEEWRTDCFYVAAH
HHHCCCHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCCHHHHCCHHHHCCHHHHHHHH
LDYLERTYWQTRYSISFPRLRPCAGALQPKSVMSDKQLVQLICAYRLFNPEVELSLSTRE
HHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHCCCCEEEEECCHH
SALFRDNVLPLGITSMSAASKTQPGGYAMPDVELEQFEISDERSAAEVEQMIRSKGFDPV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHCCCCHH
WRDWHAAYSGARFS
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SFVEHFNQLNWDDIRLSIYSKTALDVERALSKNKLDLEDFKALISPAAEHYLEAMAQRS
CHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YALTRKRFGNTLSLYIPLYLSNLCANACTYCGFSMENRLKRRTLTVQEIYAEMAAIKAMK
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FDSVLLVTGEHEGKVGMNYFRQVLPQIKRAFNYLAMEVQPLSQEHYQELKLLGLDAVMVY
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCHHHEEE
QETYHPATYAQHHLRGNKTNFNYRLETPDRLARAGIDKIGIGALIGLEEWRTDCFYVAAH
HHHCCCHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCCHHHHCCHHHHCCHHHHHHHH
LDYLERTYWQTRYSISFPRLRPCAGALQPKSVMSDKQLVQLICAYRLFNPEVELSLSTRE
HHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHCCCCEEEEECCHH
SALFRDNVLPLGITSMSAASKTQPGGYAMPDVELEQFEISDERSAAEVEQMIRSKGFDPV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHCCCCHH
WRDWHAAYSGARFS
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]