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The map label for this gene is mdtL [H]

Identifier: 27364395

GI number: 27364395

Start: 960363

End: 961523

Strand: Reverse

Name: mdtL [H]

Synonym: VV1_0956

Alternate gene names: 27364395

Gene position: 961523-960363 (Counterclockwise)

Preceding gene: 27364398

Following gene: 27364394

Centisome position: 29.3

GC content: 51.77

Gene sequence:

>1161_bases
ATGGCGTTGTTAACCTTGCTTGTGTTGTTCAGCCCGTTGGCAATTGATATTTATCTTCCCGCGTTGCCACTTATTTCCAA
TACCTTTTCGGTTGAACACGCATTAGCACAAGACACGATTACTTGGTTCCTATTTGCGATGGGCGTCGGTCAACTGTTTG
CCGGTCCGTTAGCAGATAAACTCGGACGTCGAACTGTTGCATTGGGCGGTATCACCATCTATGCACTGAGCGCTTTATTG
GCGTGGAGCGCGCAGAATATTGAATGGTTGTTGGTATCCCGTCTGCTACAAGGCTTAGGTGCGTGTGCAACATCGGTAGC
AGCCTTTGCGACAGTTCGCGATATTTTCGGCCCAGAGAAAAGTGGCAAAATGATCAGCTATCTTAACGGGGCCATTTGTT
TCATTCCGGCGCTTGCCCCCATTCTGGGTAGCTGGCTAACCCAGCAGTTTGGCTGGCGCGCTAACTTCAGTTTTATGGCG
GGCTTTGCCGTTGTCGTTGGCACGCTGATGCTGTTTAACATGCGCGAGAGCAACCCGGCGACGGAAAAACAAGCGGTGTT
TAAACTCAGCCGCTACTGGGCCGTGTTGAGTACCCCTTCTTTTGTTTTCCATGCCACGCTGTGTTTGCTGGCGATGGCGG
TGATCCTCGCTTACGTAACCTCAGCACCTGTGGTGTTGATGGAAGGGCTTGGCCTAACCATGAACCAATTTACTTTCTGG
TTTGGTGTCAACGCGGCGATCAATATCGCGGCTTGTATGCTGGCACCTAAGTTCATGGATAAATGGGGTACTCACAATAC
ACTGGTGGTGGGCGTGGTGACTCTGGCGATCGCTGGCGTAGTCATGTTGGTGATGGCAGAAACCGCGACGCCATTTGCCT
TTATGTTGCCTATCTTCCTGTCATCCGTCGGCTTTGCTTGGATTCTTGGTGCCGCCGCAGGTAAAGCGTTAGAGCCATTT
GGCGATAAAGCCGGCACGGCTGCCGCACTGCTGGGCTTATTCCAAATGAGTGGTGCGGGTCTGTTAGTGGGCACCATGCA
GCGTTTTGCGTTAGAACCTCAACTGATGATTGCGATTCAAATGTGGCTGCTGGTTCCGGCATTAGGGATTCTGTTTTCAA
AATTGGGCCGCCAGTGGCACAGCAAAGGCGTGGTGGCTTAA

Upstream 100 bases:

>100_bases
TCATTTCCGTTCATATCACGAGGCGGTTAAACTCTCATCAAAGTTCTTGTTCAATTTGGAGTGTTTTGTGTCAGGTCAGA
GCCAAACCAGTAAATTACAA

Downstream 100 bases:

>100_bases
CCACGCAACGCCAATTTAGAGTTTTACTGTATGGCGAAGCCGTGTATATTTGTCGCTCACTCCACCATTCGACACTTAAC
GGAATTGATAGGTAATGTCG

Product: putative multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MALLTLLVLFSPLAIDIYLPALPLISNTFSVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTVALGGITIYALSALL
AWSAQNIEWLLVSRLLQGLGACATSVAAFATVRDIFGPEKSGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMA
GFAVVVGTLMLFNMRESNPATEKQAVFKLSRYWAVLSTPSFVFHATLCLLAMAVILAYVTSAPVVLMEGLGLTMNQFTFW
FGVNAAINIAACMLAPKFMDKWGTHNTLVVGVVTLAIAGVVMLVMAETATPFAFMLPIFLSSVGFAWILGAAAGKALEPF
GDKAGTAAALLGLFQMSGAGLLVGTMQRFALEPQLMIAIQMWLLVPALGILFSKLGRQWHSKGVVA

Sequences:

>Translated_386_residues
MALLTLLVLFSPLAIDIYLPALPLISNTFSVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTVALGGITIYALSALL
AWSAQNIEWLLVSRLLQGLGACATSVAAFATVRDIFGPEKSGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMA
GFAVVVGTLMLFNMRESNPATEKQAVFKLSRYWAVLSTPSFVFHATLCLLAMAVILAYVTSAPVVLMEGLGLTMNQFTFW
FGVNAAINIAACMLAPKFMDKWGTHNTLVVGVVTLAIAGVVMLVMAETATPFAFMLPIFLSSVGFAWILGAAAGKALEPF
GDKAGTAAALLGLFQMSGAGLLVGTMQRFALEPQLMIAIQMWLLVPALGILFSKLGRQWHSKGVVA
>Mature_385_residues
ALLTLLVLFSPLAIDIYLPALPLISNTFSVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTVALGGITIYALSALLA
WSAQNIEWLLVSRLLQGLGACATSVAAFATVRDIFGPEKSGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMAG
FAVVVGTLMLFNMRESNPATEKQAVFKLSRYWAVLSTPSFVFHATLCLLAMAVILAYVTSAPVVLMEGLGLTMNQFTFWF
GVNAAINIAACMLAPKFMDKWGTHNTLVVGVVTLAIAGVVMLVMAETATPFAFMLPIFLSSVGFAWILGAAAGKALEPFG
DKAGTAAALLGLFQMSGAGLLVGTMQRFALEPQLMIAIQMWLLVPALGILFSKLGRQWHSKGVVA

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=335, Percent_Identity=30.7462686567164, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788509, Length=357, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI48994889, Length=343, Percent_Identity=27.6967930029155, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082312, Length=334, Percent_Identity=26.9461077844311, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790794, Length=283, Percent_Identity=24.3816254416961, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787065, Length=193, Percent_Identity=26.4248704663212, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=160, Percent_Identity=26.875, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=165, Percent_Identity=32.1212121212121, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41286; Mature: 41155

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLTLLVLFSPLAIDIYLPALPLISNTFSVEHALAQDTITWFLFAMGVGQLFAGPLADK
CHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRRTVALGGITIYALSALLAWSAQNIEWLLVSRLLQGLGACATSVAAFATVRDIFGPEK
HCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMAGFAVVVGTLMLFNMRESNPA
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TEKQAVFKLSRYWAVLSTPSFVFHATLCLLAMAVILAYVTSAPVVLMEGLGLTMNQFTFW
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHE
FGVNAAINIAACMLAPKFMDKWGTHNTLVVGVVTLAIAGVVMLVMAETATPFAFMLPIFL
EECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSVGFAWILGAAAGKALEPFGDKAGTAAALLGLFQMSGAGLLVGTMQRFALEPQLMIAIQ
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHH
MWLLVPALGILFSKLGRQWHSKGVVA
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALLTLLVLFSPLAIDIYLPALPLISNTFSVEHALAQDTITWFLFAMGVGQLFAGPLADK
HHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRRTVALGGITIYALSALLAWSAQNIEWLLVSRLLQGLGACATSVAAFATVRDIFGPEK
HCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGKMISYLNGAICFIPALAPILGSWLTQQFGWRANFSFMAGFAVVVGTLMLFNMRESNPA
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TEKQAVFKLSRYWAVLSTPSFVFHATLCLLAMAVILAYVTSAPVVLMEGLGLTMNQFTFW
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHE
FGVNAAINIAACMLAPKFMDKWGTHNTLVVGVVTLAIAGVVMLVMAETATPFAFMLPIFL
EECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSVGFAWILGAAAGKALEPFGDKAGTAAALLGLFQMSGAGLLVGTMQRFALEPQLMIAIQ
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHH
MWLLVPALGILFSKLGRQWHSKGVVA
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA