Click here to switch to the map view.

The map label for this gene is epsK [H]

Identifier: 27364318

GI number: 27364318

Start: 878515

End: 879537

Strand: Reverse

Name: epsK [H]

Synonym: VV1_0870

Alternate gene names: 27364318

Gene position: 879537-878515 (Counterclockwise)

Preceding gene: 27364319

Following gene: 326423780

Centisome position: 26.8

GC content: 50.34

Gene sequence:

>1023_bases
ATGGCTAAATCGAACCGCTCGCGCCAATCTGGAGTGGCCCTGATCATCATTTTGCTGTTGCTGGCCATTATGGCCACCAT
CGCCGGCAGTATGTCTGAGCGTTTGTTTGCACAGTTTAAGCGTGGCACTAACCAGATCAGCTATCAGCAAGCGTACTGGT
ACAGCATTGGCGTGGAAGCGTTGGCGAAGGTCGGTATTGAGCAAAGTTACAAAGACAGTGACACCATTAACTTAAACCAG
CCTTGGGCCTTGGAAGAGAAAACCTATCCCTTGGACTATGGCCAAGCGAAAGGACGCATTTTTGATAAGCAAGCGTGCTT
TAATCTCAATGCCCTCTCTCCCGTGGTGATGCAAGACGCAGCGGTAGAGCGTCCTTATTTGGTGAAAGTCTTTCGTGCTT
TGCTCGAAGAGTCAGGCGTTGAAAGTTATGAATCCGAAGTGATTGCCGACTCCATGTGGGAGTTTGTCGATCCCAATGAC
GCGGTGCGCTCTTCTTCTGGGGTGGAAGACTCGACTTATGAAGGGATGAAACCCGCCTATTTGGCGGCGAATGGGTACAT
TGCTGATAGCAGTGAACTGCGCGCGGTTTACCAAGTGTCGGGTGAAATGATGGCAACGCTGCAGCCTTTGGTCTGTGCTT
TACCGACCGATGAGTGGCGCTTGAACGTCAATACCATCAGCGCTGAGCAAGCCGCTTTGCTGGTGGCGCTGTTTTCGCCT
GCGTTGAGTGAATCTGATGCCAAGCAACTGATAGAAAAACGCCCCTTTGATGGCTGGGCGAACATTGATGCTTTTCTTTC
AGAGTCTGAAATGGCGGGCATCAGCGAAGAAATCAAAAAACTAGCAAAAGCGTATCTTGGCGTTGATAGCCGATACTTTG
AATTGGATGCACAGGTGTTAGTCGAGGACTCTCGGGTGAGAATTCGCAGCCTTATGTACAGTAAAAACAGAGAAACCGTG
ACCGTTGTGCGTCGCCGTTTTGGAGGAATCAGTGAGCGAGTTTCTGACCGTTCGGCTGAGTAG

Upstream 100 bases:

>100_bases
AAGATCGCGCGAACCTACCTCACTCCAGAGGGGAATTTGCAAAAACAAGCGTCAGGTAGCGAAGACTCCGGGCAAAACAA
TAGTGGTGGAGCAGGCAACA

Downstream 100 bases:

>100_bases
CGAACAATATAGCCCCATTCCTTGGTTGGTTTGGTCATCAAGCCAACAAGAAGTGATCGCCAGTGGGGAGTTGTCGGATT
GGCAACAACTGGACGATCTC

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEALAKVGIEQSYKDSDTINLNQ
PWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPND
AVRSSSGVEDSTYEGMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALLVALFSP
ALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVLVEDSRVRIRSLMYSKNRETV
TVVRRRFGGISERVSDRSAE

Sequences:

>Translated_340_residues
MAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEALAKVGIEQSYKDSDTINLNQ
PWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPND
AVRSSSGVEDSTYEGMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALLVALFSP
ALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVLVEDSRVRIRSLMYSKNRETV
TVVRRRFGGISERVSDRSAE
>Mature_339_residues
AKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEALAKVGIEQSYKDSDTINLNQP
WALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDAAVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPNDA
VRSSSGVEDSTYEGMKPAYLAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALLVALFSPA
LSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVLVEDSRVRIRSLMYSKNRETVT
VVRRRFGGISERVSDRSAE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=300, Percent_Identity=30, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 37893; Mature: 37762

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LAKVGIEQSYKDSDTINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDA
HHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCCHHHHHHH
AVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPNDAVRSSSGVEDSTYEGMKPAY
HCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEE
LAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALLVALFSP
EEECCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHH
ALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVL
HCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECHHHE
VEDSRVRIRSLMYSKNRETVTVVRRRFGGISERVSDRSAE
ECHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure 
AKSNRSRQSGVALIIILLLLAIMATIAGSMSERLFAQFKRGTNQISYQQAYWYSIGVEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LAKVGIEQSYKDSDTINLNQPWALEEKTYPLDYGQAKGRIFDKQACFNLNALSPVVMQDA
HHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCCHHHHHHH
AVERPYLVKVFRALLEESGVESYESEVIADSMWEFVDPNDAVRSSSGVEDSTYEGMKPAY
HCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEE
LAANGYIADSSELRAVYQVSGEMMATLQPLVCALPTDEWRLNVNTISAEQAALLVALFSP
EEECCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHH
ALSESDAKQLIEKRPFDGWANIDAFLSESEMAGISEEIKKLAKAYLGVDSRYFELDAQVL
HCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECHHHE
VEDSRVRIRSLMYSKNRETVTVVRRRFGGISERVSDRSAE
ECHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]