Click here to switch to the map view.

The map label for this gene is murA

Identifier: 27364136

GI number: 27364136

Start: 701967

End: 703232

Strand: Reverse

Name: murA

Synonym: VV1_0679

Alternate gene names: 27364136

Gene position: 703232-701967 (Counterclockwise)

Preceding gene: 27364137

Following gene: 27364135

Centisome position: 21.43

GC content: 51.03

Gene sequence:

>1266_bases
ATGGAAAAGTTCCGTGTAATTGGGTCGACTCAGCCATTGGTGGGTGAGGTGACTATTTCAGGTGCCAAAAACGCTGCACT
GCCGATTTTATTTGCGTCGATTCTGGCTGAAGAGCCAGTTGAAGTGGCCAATGTGCCGCATCTTCGAGATATTGATACCA
CCATGGAGCTGCTAAAGCGCCTTGGTGCCAAAGTGGAACGCAATGGTTCCGTTCATGTTGACCCTAGCTCTATCGATGAA
TATTGCGCGCCTTATGATTTGGTGAAAACCATGCGTGCATCGATCTGGGCATTAGGGCCGCTTGTTGCTCGTTTCGGTCA
AGGACAAGTGTCTCTGCCGGGGGGTTGTGCGATCGGTGCTCGTCCCGTGGATTTACACATCACTGGCCTTGAGCAACTAG
GTGCCACCATCACATTAGAAGATGGTTACGTTAAAGCGCATGTCGACGGCCGTTTACAAGGCGCACACATTGTCATGGAC
AAAGTGAGCGTAGGTGCCACGATCACCATCATGTGTGCAGCCACACTTGCCGAAGGCACGACGGTTTTAGATAACGCAGC
GCGTGAGCCAGAAATTGTCGATACTGCGAAATTCCTCAACACGCTTGGGGCTAAAATCAGCGGTGCAGGCACTGACACCA
TCACCATTGAGGGCGTTGAACGTCTAGGTGGTGGTAAGCATGCCGTGGTGGCAGACCGCATCGAAACGGGGACTTTCCTT
GTTGCTGCAGCGGTGTCTGGCGGTAAAGTCGTTTGCCACAATACGCAAGCGCATTTACTTGAAGCCGTGTTGGCGAAGTT
GGAAGAAGCGGGCGCCTTGGTTGAAACTGGTGAAGATTGGATTTCTGTCGATATGACAGGTCGAGAGCTCAAAGCGGTCA
ATATCCGCACGGCGCCTCACCCGGGTTTCCCGACCGATATGCAAGCACAGTTTACCTTGCTGAATATGATGGCGAAAGGC
GGTGGTGTGATCACTGAAACCATCTTTGAAAACCGTTTCATGCATGTTCCAGAGCTAAAACGTATGGGTGCAAAAGCGGA
GATTGAAGGTAACACTGTCATCTGTGGTGACGTGGAGCGTTTGAGCGCAGCACAAGTTATGGCGACCGATCTACGAGCGT
CAGCCAGCTTAGTGATTGCAGGTTGTATCGCTAAGGGTGAAACCATCGTTGATCGCATTTACCACATTGACCGTGGCTAC
GACAAAATCGAGAACAAGCTTAATGCACTGGGTGCAAAGATTGAGCGTTTTCGTGAGTCAAACTAA

Upstream 100 bases:

>100_bases
CTACATCCAACGCAATGATATTCATGCCTTGTCGATCAAAGCATACACGCCTGCCGAATGGGCACGTGACAAGAAACTGA
TGTCTCTTTAAGGTTTTATA

Downstream 100 bases:

>100_bases
ACTACGTTGATACCAATATAGCCGAAACACAGGTTTCGGCTTTTTTATATTTTAGAAGCTGTTTTTGTCAGAGAAGTAGA
GCGTGGCTTTCAATATCTTG

Product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Products: NA

Alternate protein names: Enoylpyruvate transferase; UDP-N-acetylglucosamine enolpyruvyl transferase; EPT

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MEKFRVIGSTQPLVGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLKRLGAKVERNGSVHVDPSSIDE
YCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGARPVDLHITGLEQLGATITLEDGYVKAHVDGRLQGAHIVMD
KVSVGATITIMCAATLAEGTTVLDNAAREPEIVDTAKFLNTLGAKISGAGTDTITIEGVERLGGGKHAVVADRIETGTFL
VAAAVSGGKVVCHNTQAHLLEAVLAKLEEAGALVETGEDWISVDMTGRELKAVNIRTAPHPGFPTDMQAQFTLLNMMAKG
GGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVERLSAAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGY
DKIENKLNALGAKIERFRESN

Sequences:

>Translated_421_residues
MEKFRVIGSTQPLVGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLKRLGAKVERNGSVHVDPSSIDE
YCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGARPVDLHITGLEQLGATITLEDGYVKAHVDGRLQGAHIVMD
KVSVGATITIMCAATLAEGTTVLDNAAREPEIVDTAKFLNTLGAKISGAGTDTITIEGVERLGGGKHAVVADRIETGTFL
VAAAVSGGKVVCHNTQAHLLEAVLAKLEEAGALVETGEDWISVDMTGRELKAVNIRTAPHPGFPTDMQAQFTLLNMMAKG
GGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVERLSAAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGY
DKIENKLNALGAKIERFRESN
>Mature_421_residues
MEKFRVIGSTQPLVGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLKRLGAKVERNGSVHVDPSSIDE
YCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGARPVDLHITGLEQLGATITLEDGYVKAHVDGRLQGAHIVMD
KVSVGATITIMCAATLAEGTTVLDNAAREPEIVDTAKFLNTLGAKISGAGTDTITIEGVERLGGGKHAVVADRIETGTFL
VAAAVSGGKVVCHNTQAHLLEAVLAKLEEAGALVETGEDWISVDMTGRELKAVNIRTAPHPGFPTDMQAQFTLLNMMAKG
GGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVERLSAAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGY
DKIENKLNALGAKIERFRESN

Specific function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine

COG id: COG0766

COG function: function code M; UDP-N-acetylglucosamine enolpyruvyl transferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family. MurA subfamily

Homologues:

Organism=Escherichia coli, GI1789580, Length=418, Percent_Identity=78.2296650717703, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURA_VIBVU (Q8DEB6)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759664.1
- ProteinModelPortal:   Q8DEB6
- SMR:   Q8DEB6
- GeneID:   1177627
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0679
- HOGENOM:   HBG482701
- OMA:   MVKTMRA
- ProtClustDB:   PRK09369
- BioCyc:   VVUL216895:VV1_0679-MONOMER
- BRENDA:   2.5.1.7
- GO:   GO:0005737
- HAMAP:   MF_00111
- InterPro:   IPR001986
- InterPro:   IPR013792
- InterPro:   IPR005750
- Gene3D:   G3DSA:3.65.10.10
- PANTHER:   PTHR21090:SF4
- TIGRFAMs:   TIGR01072

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.7

Molecular weight: Translated: 44708; Mature: 44708

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: ACT_SITE 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFRVIGSTQPLVGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLKR
CCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHH
LGAKVERNGSVHVDPSSIDEYCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGA
HCCCEECCCCEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
RPVDLHITGLEQLGATITLEDGYVKAHVDGRLQGAHIVMDKVSVGATITIMCAATLAEGT
EEEEEEEECHHHHCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEHHHHCCC
TVLDNAAREPEIVDTAKFLNTLGAKISGAGTDTITIEGVERLGGGKHAVVADRIETGTFL
HHHHCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEEHHHCCCCCEEEEEECCCCCEEE
VAAAVSGGKVVCHNTQAHLLEAVLAKLEEAGALVETGEDWISVDMTGRELKAVNIRTAPH
EEEECCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCEEEEEEEEECCC
PGFPTDMQAQFTLLNMMAKGGGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVER
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCHHHHCCCCEEECCCEEEECCHHH
LSAAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGYDKIENKLNALGAKIERFRES
HHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
N
C
>Mature Secondary Structure
MEKFRVIGSTQPLVGEVTISGAKNAALPILFASILAEEPVEVANVPHLRDIDTTMELLKR
CCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHH
LGAKVERNGSVHVDPSSIDEYCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCAIGA
HCCCEECCCCEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
RPVDLHITGLEQLGATITLEDGYVKAHVDGRLQGAHIVMDKVSVGATITIMCAATLAEGT
EEEEEEEECHHHHCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEHHHHCCC
TVLDNAAREPEIVDTAKFLNTLGAKISGAGTDTITIEGVERLGGGKHAVVADRIETGTFL
HHHHCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEEHHHCCCCCEEEEEECCCCCEEE
VAAAVSGGKVVCHNTQAHLLEAVLAKLEEAGALVETGEDWISVDMTGRELKAVNIRTAPH
EEEECCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCEEEEEEEEECCC
PGFPTDMQAQFTLLNMMAKGGGVITETIFENRFMHVPELKRMGAKAEIEGNTVICGDVER
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCHHHHCCCCEEECCCEEEECCHHH
LSAAQVMATDLRASASLVIAGCIAKGETIVDRIYHIDRGYDKIENKLNALGAKIERFRES
HHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA