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The map label for this gene is yhcK [H]

Identifier: 27364103

GI number: 27364103

Start: 664706

End: 666586

Strand: Reverse

Name: yhcK [H]

Synonym: VV1_0646

Alternate gene names: 27364103

Gene position: 666586-664706 (Counterclockwise)

Preceding gene: 229220646

Following gene: 27364100

Centisome position: 20.31

GC content: 45.88

Gene sequence:

>1881_bases
GTGAAAATCAGAAAGTTACTCGGCATTCTCATTTTGACCAGCGCTTTTTTCGCTGCACTCATTGCTATCTACTATCAGGA
TAAATACCAAGATCTGCTCGACGCCAATATTGAAAACACCGCGAAAGAAGCTCTTAATCAGCTCTCCTACACCGAGCGCG
AATACAACAACCTGTATGATCAAGTTGCCTCGGTATTACAACTACTGACTTATAACCAAACCTTGACGGATTTCGTCGTT
GATCCCAACCCTCAATCGGAAAGGGCGTTAATGTCTTTGTGGGAATCTGTGGTGGTCAATCAAAAGTGGTATACCGAAAT
CCGCTTGTTGGACACGGAAGGGATGGAGCAGTTGCGCCTCAATTACGACGTAAGCCGTAATTACTTTTTCCCTGCGGCGA
AGATGCAAAACGATGCAAGCGAAGCCTATTTTGTCTATGCCAAGACACTCGAAAATAGCGAGATCGGGGCATGGGGAATC
ACGTTAGACACGCGAGATGAGCAATTAATTAAACCTTATGTGCCGGTTATTTCTATTTTTACTCCAGTCACGATACGTGG
TGAGCGCGAAGGGTATTTGGCGCTAAATCTGAATGTGGATTACATCGCCGATCGGTTGAATTACTCACCCGTAAAAGATT
TTGACATTGAGATCGTCAATGATCGTGGCTACTTCGTCATCAGTGAGGAAGAAGAAAAGCAGTTTGGTGACTTGCTGCCA
GAGCGCGCGGCCTTTCGTTTCAGTGATCAATATCCGCAAGCTTGGCTGCAGTTTAGACACCAGAAAGCGGGTTACAGCTA
CGATGAGCAGGATTTAGTGGTTCATAACAGTGTTAGCTTTGGCCAAGACAGGGATCTCTACTTGCTGATCGATATGGATC
AGCAGCAGTTAATGGAACGCTCGAAAATTGCCGTGAATGATCTTACGCGTGAAGCACTCTTTGTGTTGTGTTTGATCCTA
GTTGTCGCACTGCCTTCTCTGACGATTGTACTGCACTATCGCCAGCGCAATTTGGAAAGTAAATTGGCCAGGGCGGCTTT
GAATGGCATGACCGCCGTGATGATTTCAGACACTTCTCACCGCATCATTCTTGTGAATCATGAATTCGAAGTGATGTTTG
GTCATCAGGGAGATAAGATTGAAGGGAAAAGTGCTCAACAACTGATCTTTTTGAATGACGAATTAGAAACCGTGATCAAA
GCATGGAGCGAGTTACAAAAGAACGAGGTATGGGAAGGGGAGATCCGTTGCCAAACCAAGCTCAACCACATCATCACAGT
GATCATGCGTATTCAGGCGGTGAAAAACTCTCTCAATCAAGTGAGCTACTACATTACTTCGCTGGTGGATATCAGTGCGC
GCAAAGAACTTGAGGAACGTTTGAGAAACTTAAGTGAGCGTGATGAACTGACACAAATCTGGAACCGACGTAAATTTGAA
CAGCAGCTTGCTCAACATACCAGCTTGATCGAGCGCTATCCAGCGACGCCTATGGCATGTTTAGCCTTAGTCGATATAGA
TTATTTTAAGCGAGTGAATGATGAGCAAGGCCATGATGAAGGTGATCGTGTGATACGCGAAGTGGCTAAGATTCTTCAAA
ATGGCTTGAGAAACACCGATGTCTTAGCGCGCGTTGGTGGTGAAGAGTTTGCCATCATCATGCCACATACCACCGTTTCC
GCCGCGGAAGTGGTGTTAAATCGTCTGCGTGTCGCAGTGTCGATTTCCAAACAAGTCGATGTCACGATTAGCGTGGGCTA
TACCGAACTCACCTCCAACAGCACCCTCAGTTATAAGTGTGCTGATATTGCCTTGTACGACTCTAAAAGCTCTGGTCGCA
ACAAAGTCTCCCTCTGCTACAACGATGAAGATCTCGCTTAA

Upstream 100 bases:

>100_bases
GATTCTACAATCAATCTGTAGTGCCTGATGGCGATTAAATTGACTACTATCTATGCTATACTCCCGCGGTATTCTTCTCC
CTGATAGGTAAAATTTACGA

Downstream 100 bases:

>100_bases
TCCGCTACGATGGAATTGTAACGAGATATATACAGTTAGACTAAAGTCTAATATGTGAGCAAAATTGAACTAAAATGGTT
ATTAAGTAAACTGTCACCCC

Product: sensory box/GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKIRKLLGILILTSAFFAALIAIYYQDKYQDLLDANIENTAKEALNQLSYTEREYNNLYDQVASVLQLLTYNQTLTDFVV
DPNPQSERALMSLWESVVVNQKWYTEIRLLDTEGMEQLRLNYDVSRNYFFPAAKMQNDASEAYFVYAKTLENSEIGAWGI
TLDTRDEQLIKPYVPVISIFTPVTIRGEREGYLALNLNVDYIADRLNYSPVKDFDIEIVNDRGYFVISEEEEKQFGDLLP
ERAAFRFSDQYPQAWLQFRHQKAGYSYDEQDLVVHNSVSFGQDRDLYLLIDMDQQQLMERSKIAVNDLTREALFVLCLIL
VVALPSLTIVLHYRQRNLESKLARAALNGMTAVMISDTSHRIILVNHEFEVMFGHQGDKIEGKSAQQLIFLNDELETVIK
AWSELQKNEVWEGEIRCQTKLNHIITVIMRIQAVKNSLNQVSYYITSLVDISARKELEERLRNLSERDELTQIWNRRKFE
QQLAQHTSLIERYPATPMACLALVDIDYFKRVNDEQGHDEGDRVIREVAKILQNGLRNTDVLARVGGEEFAIIMPHTTVS
AAEVVLNRLRVAVSISKQVDVTISVGYTELTSNSTLSYKCADIALYDSKSSGRNKVSLCYNDEDLA

Sequences:

>Translated_626_residues
MKIRKLLGILILTSAFFAALIAIYYQDKYQDLLDANIENTAKEALNQLSYTEREYNNLYDQVASVLQLLTYNQTLTDFVV
DPNPQSERALMSLWESVVVNQKWYTEIRLLDTEGMEQLRLNYDVSRNYFFPAAKMQNDASEAYFVYAKTLENSEIGAWGI
TLDTRDEQLIKPYVPVISIFTPVTIRGEREGYLALNLNVDYIADRLNYSPVKDFDIEIVNDRGYFVISEEEEKQFGDLLP
ERAAFRFSDQYPQAWLQFRHQKAGYSYDEQDLVVHNSVSFGQDRDLYLLIDMDQQQLMERSKIAVNDLTREALFVLCLIL
VVALPSLTIVLHYRQRNLESKLARAALNGMTAVMISDTSHRIILVNHEFEVMFGHQGDKIEGKSAQQLIFLNDELETVIK
AWSELQKNEVWEGEIRCQTKLNHIITVIMRIQAVKNSLNQVSYYITSLVDISARKELEERLRNLSERDELTQIWNRRKFE
QQLAQHTSLIERYPATPMACLALVDIDYFKRVNDEQGHDEGDRVIREVAKILQNGLRNTDVLARVGGEEFAIIMPHTTVS
AAEVVLNRLRVAVSISKQVDVTISVGYTELTSNSTLSYKCADIALYDSKSSGRNKVSLCYNDEDLA
>Mature_626_residues
MKIRKLLGILILTSAFFAALIAIYYQDKYQDLLDANIENTAKEALNQLSYTEREYNNLYDQVASVLQLLTYNQTLTDFVV
DPNPQSERALMSLWESVVVNQKWYTEIRLLDTEGMEQLRLNYDVSRNYFFPAAKMQNDASEAYFVYAKTLENSEIGAWGI
TLDTRDEQLIKPYVPVISIFTPVTIRGEREGYLALNLNVDYIADRLNYSPVKDFDIEIVNDRGYFVISEEEEKQFGDLLP
ERAAFRFSDQYPQAWLQFRHQKAGYSYDEQDLVVHNSVSFGQDRDLYLLIDMDQQQLMERSKIAVNDLTREALFVLCLIL
VVALPSLTIVLHYRQRNLESKLARAALNGMTAVMISDTSHRIILVNHEFEVMFGHQGDKIEGKSAQQLIFLNDELETVIK
AWSELQKNEVWEGEIRCQTKLNHIITVIMRIQAVKNSLNQVSYYITSLVDISARKELEERLRNLSERDELTQIWNRRKFE
QQLAQHTSLIERYPATPMACLALVDIDYFKRVNDEQGHDEGDRVIREVAKILQNGLRNTDVLARVGGEEFAIIMPHTTVS
AAEVVLNRLRVAVSISKQVDVTISVGYTELTSNSTLSYKCADIALYDSKSSGRNKVSLCYNDEDLA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=36.144578313253, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=37.6470588235294, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=35.7954545454545, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=33.5195530726257, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788085, Length=206, Percent_Identity=34.9514563106796, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=31.5508021390374, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787541, Length=181, Percent_Identity=35.3591160220994, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI145693134, Length=199, Percent_Identity=30.1507537688442, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=30.8510638297872, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=29.1390728476821, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 72036; Mature: 72036

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRKLLGILILTSAFFAALIAIYYQDKYQDLLDANIENTAKEALNQLSYTEREYNNLYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QVASVLQLLTYNQTLTDFVVDPNPQSERALMSLWESVVVNQKWYTEIRLLDTEGMEQLRL
HHHHHHHHHHCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHEE
NYDVSRNYFFPAAKMQNDASEAYFVYAKTLENSEIGAWGITLDTRDEQLIKPYVPVISIF
EEECCCCEECCHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
TPVTIRGEREGYLALNLNVDYIADRLNYSPVKDFDIEIVNDRGYFVISEEEEKQFGDLLP
CCEEEECCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHCCC
ERAAFRFSDQYPQAWLQFRHQKAGYSYDEQDLVVHNSVSFGQDRDLYLLIDMDQQQLMER
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHH
SKIAVNDLTREALFVLCLILVVALPSLTIVLHYRQRNLESKLARAALNGMTAVMISDTSH
HHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEHHHHCHHHHHHHHHHCCCEEEEEECCCC
RIILVNHEFEVMFGHQGDKIEGKSAQQLIFLNDELETVIKAWSELQKNEVWEGEIRCQTK
EEEEEECCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEHHHH
LNHIITVIMRIQAVKNSLNQVSYYITSLVDISARKELEERLRNLSERDELTQIWNRRKFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QQLAQHTSLIERYPATPMACLALVDIDYFKRVNDEQGHDEGDRVIREVAKILQNGLRNTD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
VLARVGGEEFAIIMPHTTVSAAEVVLNRLRVAVSISKQVDVTISVGYTELTSNSTLSYKC
HHHEECCCEEEEEECCCCHHHHHHHHHHHHHHEEECCEEEEEEEECEEEECCCCEEEEEE
ADIALYDSKSSGRNKVSLCYNDEDLA
EEEEEECCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKIRKLLGILILTSAFFAALIAIYYQDKYQDLLDANIENTAKEALNQLSYTEREYNNLYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QVASVLQLLTYNQTLTDFVVDPNPQSERALMSLWESVVVNQKWYTEIRLLDTEGMEQLRL
HHHHHHHHHHCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHEE
NYDVSRNYFFPAAKMQNDASEAYFVYAKTLENSEIGAWGITLDTRDEQLIKPYVPVISIF
EEECCCCEECCHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHH
TPVTIRGEREGYLALNLNVDYIADRLNYSPVKDFDIEIVNDRGYFVISEEEEKQFGDLLP
CCEEEECCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHCCC
ERAAFRFSDQYPQAWLQFRHQKAGYSYDEQDLVVHNSVSFGQDRDLYLLIDMDQQQLMER
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHH
SKIAVNDLTREALFVLCLILVVALPSLTIVLHYRQRNLESKLARAALNGMTAVMISDTSH
HHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEHHHHCHHHHHHHHHHCCCEEEEEECCCC
RIILVNHEFEVMFGHQGDKIEGKSAQQLIFLNDELETVIKAWSELQKNEVWEGEIRCQTK
EEEEEECCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEHHHH
LNHIITVIMRIQAVKNSLNQVSYYITSLVDISARKELEERLRNLSERDELTQIWNRRKFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QQLAQHTSLIERYPATPMACLALVDIDYFKRVNDEQGHDEGDRVIREVAKILQNGLRNTD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
VLARVGGEEFAIIMPHTTVSAAEVVLNRLRVAVSISKQVDVTISVGYTELTSNSTLSYKC
HHHEECCCEEEEEECCCCHHHHHHHHHHHHHHEEECCEEEEEEEECEEEECCCCEEEEEE
ADIALYDSKSSGRNKVSLCYNDEDLA
EEEEEECCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]